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Citations for Eppendorf :
301 - 350 of 465 citations for 7α 12α Dihydroxycholest 4 en 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The rest of the solution was incubated at 4°C for 1 hour on a thermomixer (Eppendorf, Hamburg, Germany) set to 1000 rpm ...
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bioRxiv - Genomics 2019Quote: ... Cells were thawed and pelleted in a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Cell pellets were resuspended in 250 μl nuclei permeabilization buffer (10 mM Tris-HCl pH 7.4 (Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant from 300 × g was transferred to new 15mL tube and then centrifuged at 2,000 × g for 10 min at 4°C (# 5720R Eppendorf). The supernatant from 2,000 × g was transferred to 30mL conical tube and then centrifuged at 10,000 × g for 10 min at 4°C (# Avanti J-E JA25-50 Beckman Coulter) ...
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bioRxiv - Bioengineering 2021Quote: ... The tube was centrifuged for 10 min at 1,000x rpm at 4°C with minimum acceleration and deceleration (5810R Centrifuge, Eppendorf). Any fluid expelled was collected into 1.5 mL tubes ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a 30-mL conical tube and centrifuged at 10,000 × g for 10 min at 4°C (#5424R Eppendorf). The supernatant filtered through a 0.22-μm polyethersulfone membrane filter (# SLGP033RS EMD Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred to a new 15-mL polypropylene tube and centrifuged at 2,000 × g for 10 min at 4°C (# 5720R Eppendorf). The supernatant was transferred to a 30-mL conical tube and centrifuged at 10,000 × g for 10 min at 4°C (#5424R Eppendorf) ...
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bioRxiv - Cell Biology 2020Quote: ... The homogenate was further incubated on ice for 45 min before centrifugation at 21,000 g and 4°C for 2 x 10 min in an Eppendorf 5424 R benchtop centrifuge (Eppendorf). Protein content of the lysate was determined by BCA assay (Thermo Fisher) ...
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bioRxiv - Genomics 2019Quote: ... Isolated brain nuclei were pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA ...
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bioRxiv - Neuroscience 2020Quote: The homogenates were pelleted at 400xg for 6 minutes at 4°C in a swing-bucket rotor centrifuge (Eppendorf). The supernatants were removed and 1 ml ice-cold DPBS (pH 7.3-7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were put on ice for 30 min and precipitates were spun down for 5 min at 20,000 g at 4°C in a tabletop centrifuge (Eppendorf). After one wash with ice-cold acetone ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The sample was subsequently centrifuged for 1 h at 11,000 x g at 4°C in an Eppendorf tabletop centrifuge 5810R (Eppendorf). The phage pellet was resuspended in 400 μl DNAseI buffer (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then centrifuged at 13,000×g for 20 min at 4 °C using an F2402 rotor in a GS15R table-top centrifuge (Eppendorf). Pellets were air dried ...
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bioRxiv - Cell Biology 2022Quote: ... protease and phosphatase inhibitors and PMSF (10 minutes on ice) and centrifuged at 13,300 rpm for 5 min at 4OC (Centrifuge 5810 R; rotor A-4-81; Eppendorf). The supernatant (whole cell lysates ...
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bioRxiv - Cell Biology 2022Quote: ... 4 mM MgCl2) was carefully injected into an individual cardiomoycyte by a glass pipette via the CellTram injector (Eppendorf). After 5 minutes dye spreading to neighboring cardiomyocytes was visualized by Zeiss Axio Examiner A1 using a 40× objective and images were taken using TILL Photonics LA software (version 2.3.0.18 ...
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bioRxiv - Plant Biology 2023Quote: ... samples (stored for 4-12 weeks) were thawed at room temperature for 15 mins and centrifuged (Eppendorf Centrifuge 5417C) at 11,000 rpm for 14 mins to extract bulk leaf sap ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were centrifuged at 4°C at 15,000 RPM for 15 minutes in a table top centrifuge (Eppendorf 5424R). The supernatant’s protein concentration was measured using Bradford’s assay (Bio-Rad #5000202) ...
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bioRxiv - Microbiology 2023Quote: ... OD660 of 0.3-0.5 mid-log) were harvested by centrifugation (5,000 x g, 5 min at 4°C) (Eppendorf centrifuge 5430R). The supernatant was immediately discarded ...
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bioRxiv - Microbiology 2023Quote: ... harvested by centrifugation (3,500 × g for 20 min at 4°C, Centrifuge 5702 R, Eppendorf North America, Framingham, MA), and suspended in an equivalent amount of 1 × PBS ...
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bioRxiv - Microbiology 2023Quote: ... The precipitated gDNA was pelleted by centrifugation (21,000 x g, 10 min at 4°C) in a tabletop centrifuge (Model 5424, Eppendorf). The supernatant was discarded by decantation ...
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bioRxiv - Neuroscience 2023Quote: ... and tubes centrifuged at 4 °C for 2 min at 12000 x g in a refrigerated microfuge (Eppendorf 5415R). The upper phase was discarded ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were centrifuged at 10,000 rpm at 4°C for 10 min and supernatants were dried under vacuum centrifugation (Eppendorf) overnight ...
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bioRxiv - Bioengineering 2023Quote: All centrifugation experiments were conducted in an Eppendorf 5702R centrifuge with a swing bucket rotor (Eppendorf, A-4-38) at 20°C ...
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bioRxiv - Microbiology 2023Quote: ... The parasite solution was pelleted at 1,200 g at 4°C for 10 min using a chilled large tabletop centrifuge (Eppendorf). The resulting pellet underwent 3 washes with intracellular buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The incubated sample was centrifuged at 20,800 g for 15 min at 4 °C (Rotor F45-30-11, Eppendorf), and the resulting supernatant was analyzed by immunoblotting using various antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... fractions numbered from 9 to 22 were processed by centrifugation (20,800 g for 30 min at 4 °C, Rotor F45-30-11, Eppendorf), and the resulting pellet was further resuspended in 160 μL of alkaline carbonate buffer (100 mM Na2CO3 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed in 20 mL of chilled phosphate-buffered saline (1X PBS, pH 7.4), and centrifuged (1500 ×g, 4 °C, 15 min) using the Centrifuge 5810R (Eppendorf). The cell pellet was subsequently resuspended in 30 mL of ice-cold RSB hypo buffer (10 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... the homogenate was centrifuged at 2500 rpm for 10 minutes at 4°C using a benchtop centrifuge (Eppendorf, USA), and the supernatant was collected.
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bioRxiv - Biochemistry 2021Quote: ... Cells were maintained in a humidified atmosphere of 5% CO2 and 37 °C and were passaged every 2-3 days into 10 cm polystyrene coated plates (Eppendorf; EP0030700112-300EA) upon reaching high density ...
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bioRxiv - Biochemistry 2022Quote: ... and samples were vortexed at 1200 rpm for 2-3 hours at room temperature (MixMate ®, Eppendorf South Pacific, Sydney, NSW Australia). Following this ...
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bioRxiv - Systems Biology 2022Quote: ... each SCN was cut into 3 pieces with a scalpel on a glass slide and transferred to a protein LoBind tube (Eppendorf, Hamburg, Germany) prefilled with 250 µL lysis buffer (100 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... were also added to each tube and incubated at 37°C for 3 h in a thermomixer (Eppendorf Thermomixer C, Hamburg, Germany). After 3 h incubation ...
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bioRxiv - Biophysics 2023Quote: ... and the device was left running for a minimum of 3 h while droplets were being collected in a 1.5 mL microcentrifuge tube (Eppendorf®, Hauppauge, NY).
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bioRxiv - Developmental Biology 2023Quote: ... the gRNA:Cas9 ribonucleoprotein complex solution was incubated at 37°C for 5 min and then backfilled into 3 microinjection needles using an Eppendorf GELoader tip (Eppendorf, Cat# 022351656). After loading embryos into the embryo holder which covered with 12.5 ppt of salinity water with 0.0001% of methylene blue ...
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bioRxiv - Biophysics 2023Quote: ... Cell growth was quantified at intervals by measuring their optical density at regular intervals (as mentioned in Figure 3) using a Biophotometer® D30 (Eppendorf, Germany). The morphology of cells at different growth stages was determined by a variety of microscopic techniques (as described in the below sections).
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bioRxiv - Plant Biology 2024Quote: ... SDS at a 1:3 (w/v) ratio and extracted by shaking 1000 RPM 95 °C for using a tabletop shaker (Eppendorf ThermoMixer F2.0). Samples were then centrifuged at 20,000 xg for 10 min at room temperature and the supernatant retained in new tubes ...
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bioRxiv - Cell Biology 2020Quote: ... we centrifuged the crude lysate at 500 g for 5 min at 4°C in a microfuge (Eppendorf, model 5424R) to remove cell debris ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM label was subsequently added and samples were incubated over night at 4°C and 350 rpm in a Thermomixer (Eppendorf). Excess label was removed by using a Sephadex G25 column (GE Healthcare Bio-Science AB ...
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bioRxiv - Bioengineering 2022Quote: ... The lysates were clarified by centrifugation at 18,500g at 4 °C for 30 minutes using tabletop centrifuge (Eppendorf Centrifuge 5810R). Soluble protein was separated from cell debris and the supernatant was passed through 5 mL HisTrap columns (Cytiva ...
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bioRxiv - Neuroscience 2021Quote: ... Single EGFP+ cells were sorted on a BD Influx equipped with a 140 µm nozzle and a cooling unit with a sample temperature of 4°C and collected into a DNA LoBind tube (Eppendorf) containing aCSF with 0.5% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... Absorbance was measured at 600 nm after every 12 h intervals till 4 to 5 days using a UV-visible spectrophotometer (Eppendorf).
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bioRxiv - Biophysics 2020Quote: ... The needle was then glued to a 6×4×2 mm Neodyn magnet (QM-06×04×02-N, Magnets4you) and attached to a motorized micromanipulator (PatchMan, Eppendorf). The magnetic needle was lowered until it touched the bottom of a dummy sample dish and raised again so that it was placed above the bottom of the sample with the very tip in focus around 100 μm above the focus of the glass surface.
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bioRxiv - Microbiology 2019Quote: ... the vials were removed from the incubator and beads were spun down at 1200 rpm using a swinging-bucket A-4-81 rotor (Eppendorf). An aliquot of 10 µl of bead suspension was carefully sampled from the bottom of the vials ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
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bioRxiv - Genetics 2020Quote: ... The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042, Eppendorf, Inc.) and the resultant supernatant fractions were stored at −80°C ...
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bioRxiv - Genomics 2021Quote: ... nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
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bioRxiv - Immunology 2021Quote: Xela DS2 (passages 120 – 130) and Xela VS2 (passages 125 – 135) (n = 4 independent trials) were seeded in a 6-well plate (Eppendorf) at a cell density of 625,000 cells/well in 1 mL of Xela complete media and allowed to adhere overnight at 26 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 180 μL aliquots of solubilized GlyT1 cell membrane were dispensed in a 4°C-cooled 96-well PCR plate (Eppendorf) in triplicates ...
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bioRxiv - Microbiology 2021Quote: ... Cell harvest was performed outside the anaerobic chamber at 3700 rpm for 12 min (Centrifuge 5920R, S-4×1000, Eppendorf) at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transferred into the anaerobic chamber and harvested for 12 min at 25°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf) outside of the anaerobic chamber ...
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bioRxiv - Neuroscience 2019Quote: ... The lysate was cleared from the insoluble fraction by centrifugation for 15 min at 10,000 rpm (11,292 x g) at 4 °C (Eppendorf 5427 R). The supernatant was retrieved and the procedure repeated until no pellet was visible after centrifugation ...