Labshake search
Citations for Eppendorf :
251 - 300 of 394 citations for 6 chloro 4 methoxyquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... Lysates were centrifuged at 4°C at 15,000 RPM for 15 minutes in a table top centrifuge (Eppendorf 5424R). The supernatant’s protein concentration was measured using Bradford’s assay (Bio-Rad #5000202) ...
-
bioRxiv - Microbiology 2023Quote: ... OD660 of 0.3-0.5 mid-log) were harvested by centrifugation (5,000 x g, 5 min at 4°C) (Eppendorf centrifuge 5430R). The supernatant was immediately discarded ...
-
bioRxiv - Microbiology 2023Quote: ... harvested by centrifugation (3,500 × g for 20 min at 4°C, Centrifuge 5702 R, Eppendorf North America, Framingham, MA), and suspended in an equivalent amount of 1 × PBS ...
-
bioRxiv - Microbiology 2023Quote: ... The precipitated gDNA was pelleted by centrifugation (21,000 x g, 10 min at 4°C) in a tabletop centrifuge (Model 5424, Eppendorf). The supernatant was discarded by decantation ...
-
bioRxiv - Neuroscience 2023Quote: ... and tubes centrifuged at 4 °C for 2 min at 12000 x g in a refrigerated microfuge (Eppendorf 5415R). The upper phase was discarded ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were centrifuged at 10,000 rpm at 4°C for 10 min and supernatants were dried under vacuum centrifugation (Eppendorf) overnight ...
-
bioRxiv - Bioengineering 2023Quote: All centrifugation experiments were conducted in an Eppendorf 5702R centrifuge with a swing bucket rotor (Eppendorf, A-4-38) at 20°C ...
-
bioRxiv - Microbiology 2023Quote: ... The parasite solution was pelleted at 1,200 g at 4°C for 10 min using a chilled large tabletop centrifuge (Eppendorf). The resulting pellet underwent 3 washes with intracellular buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... The incubated sample was centrifuged at 20,800 g for 15 min at 4 °C (Rotor F45-30-11, Eppendorf), and the resulting supernatant was analyzed by immunoblotting using various antibodies ...
-
bioRxiv - Cell Biology 2023Quote: ... fractions numbered from 9 to 22 were processed by centrifugation (20,800 g for 30 min at 4 °C, Rotor F45-30-11, Eppendorf), and the resulting pellet was further resuspended in 160 μL of alkaline carbonate buffer (100 mM Na2CO3 ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were washed in 20 mL of chilled phosphate-buffered saline (1X PBS, pH 7.4), and centrifuged (1500 ×g, 4 °C, 15 min) using the Centrifuge 5810R (Eppendorf). The cell pellet was subsequently resuspended in 30 mL of ice-cold RSB hypo buffer (10 mM NaCl ...
-
bioRxiv - Microbiology 2024Quote: ... the homogenate was centrifuged at 2500 rpm for 10 minutes at 4°C using a benchtop centrifuge (Eppendorf, USA), and the supernatant was collected.
-
bioRxiv - Cell Biology 2020Quote: ... we centrifuged the crude lysate at 500 g for 5 min at 4°C in a microfuge (Eppendorf, model 5424R) to remove cell debris ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 mM label was subsequently added and samples were incubated over night at 4°C and 350 rpm in a Thermomixer (Eppendorf). Excess label was removed by using a Sephadex G25 column (GE Healthcare Bio-Science AB ...
-
bioRxiv - Bioengineering 2022Quote: ... The lysates were clarified by centrifugation at 18,500g at 4 °C for 30 minutes using tabletop centrifuge (Eppendorf Centrifuge 5810R). Soluble protein was separated from cell debris and the supernatant was passed through 5 mL HisTrap columns (Cytiva ...
-
bioRxiv - Neuroscience 2021Quote: ... Single EGFP+ cells were sorted on a BD Influx equipped with a 140 µm nozzle and a cooling unit with a sample temperature of 4°C and collected into a DNA LoBind tube (Eppendorf) containing aCSF with 0.5% BSA ...
-
bioRxiv - Cell Biology 2022Quote: ... Absorbance was measured at 600 nm after every 12 h intervals till 4 to 5 days using a UV-visible spectrophotometer (Eppendorf).
-
bioRxiv - Microbiology 2019Quote: ... the vials were removed from the incubator and beads were spun down at 1200 rpm using a swinging-bucket A-4-81 rotor (Eppendorf). An aliquot of 10 µl of bead suspension was carefully sampled from the bottom of the vials ...
-
bioRxiv - Genomics 2021Quote: ... Nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
-
bioRxiv - Genetics 2020Quote: ... The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042, Eppendorf, Inc.) and the resultant supernatant fractions were stored at −80°C ...
-
bioRxiv - Genomics 2021Quote: ... nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
-
bioRxiv - Neuroscience 2020Quote: ... 180 μL aliquots of solubilized GlyT1 cell membrane were dispensed in a 4°C-cooled 96-well PCR plate (Eppendorf) in triplicates ...
-
bioRxiv - Microbiology 2021Quote: ... Cell harvest was performed outside the anaerobic chamber at 3700 rpm for 12 min (Centrifuge 5920R, S-4×1000, Eppendorf) at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were transferred into the anaerobic chamber and harvested for 12 min at 25°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf) outside of the anaerobic chamber ...
-
bioRxiv - Neuroscience 2019Quote: ... The lysate was cleared from the insoluble fraction by centrifugation for 15 min at 10,000 rpm (11,292 x g) at 4 °C (Eppendorf 5427 R). The supernatant was retrieved and the procedure repeated until no pellet was visible after centrifugation ...
-
bioRxiv - Biochemistry 2021Quote: ... The reactions were stopped by the addition of phosphoric acid and 4 µl of each reaction were spotted on P81 phosphocellulose papers (Whatman) using the epMotion 5070 (Eppendorf) workstation ...
-
bioRxiv - Cell Biology 2021Quote: ... was harvested by centrifugation at 300 × g for 5 min in a swing out 5804 S-4-72 rotor (Eppendorf). Baculovirus infected insect cell (BIIC ...
-
bioRxiv - Microbiology 2019Quote: ... PMNs were sedimented for 10 min with 1000 × g at 4°C on 45° fixed-angle rotor FA-45-30-11 (Eppendorf). The supernatant was filtered by gravity through sterile PVDF 5.0 µm Millex syringe filters (Merck-Millipore) ...
-
bioRxiv - Cell Biology 2021Quote: ... were vortexed 3 min at room temperature followed by centrifugation at 4,500 x g for 5 min at 4°C (Eppendorf #5010R). The supernatant fluid was poured into a chilled 2 ml screw-capped tube ...
-
bioRxiv - Cell Biology 2021Quote: ... The resuspended cilia sample was centrifuged at 16,000g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415D). The supernatant was discarded ...
-
bioRxiv - Synthetic Biology 2022Quote: The concentration and quality of the plasmid solutions were determined by diluting 1 ul of plasmid solution in 4 ul of Tris EDTA buffer and loading 3 ul of the diluted solution on a μCuvette G1 (Eppendorf). Optical densities were measured at 260 nm and 280 nm using a BioSpectrophotometer and Fluorimeter (Eppendorf) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasma was defrosted and then centrifuged at 2000 rpm (805 × g) for 4 min in a 5810 R centrifuge (Eppendorf) to remove possible particulate matter ...
-
bioRxiv - Immunology 2020Quote: ... The cultures were placed on ice for 10 minutes to slow growth then centrifuged at 3,000 × g for 10 minutes at 4°C (Eppendorf 5810R). The supernatants were discarded and the pellets were resuspended in fresh RPMI 10mM glucose until a McFarland of 5.0 (∼109 CFU/mL ...
-
bioRxiv - Systems Biology 2020Quote: ... A fixed volume of 100 μL cells was used to inoculate 50 mL preculture M9 +40 mM MOPS media with 4 g/L glucose which was grown overnight in a shake flask at 200 rpm in a 37°C (Eppendorf) incubator ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Homogenates were centrifuged at 1000 rpm for 5 min at 4°C in a table centrifuge (5424 R, Eppendorf, Germany). The supernatant was transferred to a new tube and again centrifuged at 4°C for 5 min at 1400 rpm ...
-
bioRxiv - Neuroscience 2019Quote: ... Excised gel slices were incubated with 50% acetonitrile/50mM ammonium bicarbonate for 2 h at room temperature or 4°C overnight with gentle shaking at 600 rpm in a Thermomixer (Eppendorf), before being incubated in 100% acetonitrile for 15 min at room temperature ...
-
bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
-
bioRxiv - Bioengineering 2019Quote: ... the crude extracts were transferred into a fresh tube and centrifuged for 30 min at 15000g and 4 °C (Centrifuge 5427R, Eppendorf). The soluble supernatant (blue color ...
-
bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were then centrifuged at 400xg for 10 min (Eppendorf Centrifuge 5810R, rotor A-4-62) at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... Peptides were eluted in 20 μL of 80% acetonitrile in 0.1% TFA and were concentrated to 4 μL (Concentrator 5301, Eppendorf AG). The peptides sample was then diluted to 5 μL by 0.1% TFA for LC-MS/MS analysis.
-
bioRxiv - Cell Biology 2022Quote: ... Sorted nuclei were then centrifuged at 1000 g for 15 min (Eppendorf, 5920R; 4°C, ramp speed of 3/3), and supernatant was removed ...
-
bioRxiv - Microbiology 2022Quote: ... The cell suspension was then transferred into 2 mL tubes and centrifuged at 16 000g and 4°C (Eppendorf, Germany) to pellet the cells ...
-
bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged at 16,100 g for 20 min at 4 °C with an Eppendorf 5415R centrifuge (Eppendorf, Hamburg, Germany), sonicated for 10 s and protein amounts normalized following a bicinchoninic acid protein assay (ThermoFisher) ...
-
bioRxiv - Bioengineering 2023Quote: ... The resulting injection micropipette was backfilled with 5-10 μL of 500 μg/mL fluorescein solution and was mounted on the microinjection manipulator (InjectMan 4, Eppendorf) connected to a pneumatic microinjection pump (FemtoJet 4i ...
-
bioRxiv - Systems Biology 2023Quote: ... Homogenized islets were filtered with 30 µm filter (CellTrics, Sysmex) and pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Nuclei were resuspended in 400 µL of sort buffer (1% fatty acid-free BSA ...
-
bioRxiv - Systems Biology 2023Quote: ... were added without disturbing the pellet and nuclei were pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Supernatant was gently removed without disturbing the pellet and leaving ∼2-3 µl behind ...
-
bioRxiv - Systems Biology 2023Quote: ... and incubated on ice for 1 min before pelleting with a swinging-bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Supernatant was gently removed and ∼50 µl were left behind to increase nuclei recovery ...
-
bioRxiv - Genomics 2022Quote: ... The beads were then incubated 1 h with the anti-Myc antibody (9E10) at 4°C (Eppendorf ThermoMixer, 1,300 rpm), washed three times in PBS/0.1 % BSA and two times in lysis buffer ...
-
bioRxiv - Microbiology 2023Quote: ... The sample was vortexed for 30 seconds before being centrifuged for 15 mins at 4°C at 1739 x g (3000 rpm, Eppendorf 5810R benchtop centrifuge ...
-
bioRxiv - Biochemistry 2023Quote: ... The mixture was then incubated at 37 °C for 4 hrs under gentle orbital agitation (300 rpm on Eppendorf Thermomixer) and centrifuged at 15 000 × g for 5 min prior to SEC analysis ...