Labshake search
Citations for Eppendorf :
401 - 450 of 977 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... at a final ratio of 2.5 nmol DSS per 1 µg protein for 30 min at 37 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). After quenching by addition of ammonium bicarbonate to a final concentration of 50 mM and incubation for 10 min at 37 °C ...
-
bioRxiv - Biophysics 2021Quote: The gp15-gp16 core complex was obtained in vitro after incubation of the purified proteins for 30 min at room temperature (RT) in a thermo block with shaking (Eppendorf). The proteins were incubated using 180 pmol each at a 1:1 ratio ...
-
bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The fractions with highest concentrations (200–400 μg mL−1) were stored as 5–10 μL aliquots in Protein LoBind tubes (Eppendorf) at –80 °C.
-
bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
-
bioRxiv - Microbiology 2022Quote: ... the digested protein fraction was forced through the filter and collected in a 500 μl LoBind plate (Eppendorf, Hamburg, Germany). The filter was further washed with 100 μl 50 mM ABC followed by 100 μl H2O ...
-
bioRxiv - Genomics 2022Quote: ... and the pellet was resuspended in 500 ul Buffer CT and the mixture was transferred to a 2.0 mL Protein LoBind tube (Eppendorf # 022431102). The suspension was spun again at 16,000 x g at 4 C for 2 min and the supernatant discarded ...
-
bioRxiv - Genomics 2022Quote: ... The supernatant (∼200 ul) was transferred to a 70uM filter (Fisher # NC1444112) set in a new 1.5 mL Protein LoBind tube (Eppendorf # 022431081). The tube with the 70 uM filter was spun on a mini-centrifuge (Ohaus # FC5306 ...
-
bioRxiv - Immunology 2020Quote: ... plates were placed on magnetic plates and digestion reaction containing trypsinized peptides was transferred to a protein LoBind Eppendorf tube (Eppendorf, Cat ...
-
bioRxiv - Neuroscience 2022Quote: At time of collection asteroids were transferred to a 1.5 mL Protein LoBind Eppendorf (Eppendorf cat#022-43-108-1) and allowed to settle ...
-
bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 mL of 200 µM monomeric αSyn was incubated in fibrillation buffer (Supplementary Table S2) in a 1.5 mL Protein LoBind Eppendorf tube (Eppendorf, Germany) on an orbital shaker (PCMT Thermoshaker Grant-bio ...
-
bioRxiv - Synthetic Biology 2022Quote: Excitation and emission spectra of the fluorescent proteins were conducted using a BMG Clariostar Plus microplate reader in sealed plates (Corning, 3370; Eppendorf Masterclear real-time PCR film adhesive ...
-
bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 3000 x g for 6 min at 4 °C and the conditioned media was transferred to a Protein LoBind tube (Eppendorf) (media fraction) ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein concentration in the supernatant was determined according to the Bradford Ultra method (Bradford, 1976) on a Bio-Spectrophotometer (Eppendorf) at 595 nm ...
-
bioRxiv - Cell Biology 2023Quote: ... After a 5-minute incubation the protein concentration was determined through measurement of the OD595 with a photometer (BioPhotometer plus, Eppendorf). Finally ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Samples were then centrifuged at 14,000xg for 10 minutes to pellet insoluble debris and the resultant clarified lysate was transferred to a fresh protein LoBind tube (Eppendorf).
-
bioRxiv - Microbiology 2023Quote: ... The beads were pelleted (600 x g, 3 min) and the supernatant transferred to a fresh low protein binding tube (Eppendorf). The beads were washed with 1 x 200 µL HPLC-grade water and the washes combined with the original supernatant ...
-
bioRxiv - Molecular Biology 2024Quote: ... though the maximum volume of beads + gDNA + binding buffer should not exceed 1.4 ml for a single 1.5 ml Protein LoBind tube (Eppendorf, Cat. 30108442). Multiple tubes can be used and pooled at the restriction enzyme digest step ...
-
bioRxiv - Immunology 2022Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ∼ 0.8 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... These reactions were incubated for 2 h at 37°C with 220 rpm shaking (Eppendorf, Thermo Mixer C). AcTEV protease AL-PHA assays ...
-
bioRxiv - Microbiology 2019Quote: ... cultures were centrifuged for 2 min at 15,000 rcf using a table top Eppendorf 5424 centrifuge (Eppendorf, Germany) to disrupt chains (confirmed by bright field microscopy) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... These reactions were incubated for 2 h at 30°C with 500 rpm shaking (Eppendorf, Thermo Mixer C). MMP14 AL-PHA assays ...
-
bioRxiv - Bioengineering 2021Quote: ... After finishing every experiment RBCs were pelleted down by centrifuging at 1000 rpm for 2 min (Eppendorf, 5415C) and the supernatant absorbance was assessed and normalized using the maximum hemolysis value.
-
bioRxiv - Physiology 2021Quote: ... These were then incubated at 70°C at 1400 rpm for 2 hours on a heat shaker (Eppendorf) and spun at 2500 x g for 5 min ...
-
bioRxiv - Systems Biology 2020Quote: ... Samples were thawed shortly before mass spectrometric analysis and shaken for 2 minutes at 2000rpm (thermomixer C, Eppendorf). Peptide concentrations were measured optically at 280nm (Nanodrop 2000 ...
-
bioRxiv - Immunology 2020Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ~0.8 ...
-
bioRxiv - Cell Biology 2022Quote: ... the pellet was hydrolyzed in 3N HCl for 2 hours at 95 °C on a shaking Thermomixer (Eppendorf). The reaction was quenched with 100 % methanol containing 40 μM L-norvaline (as an internal control) ...
-
bioRxiv - Microbiology 2020Quote: ... mouth rinse-treated SARS-CoV-2 viruses were concentrated by centrifugation at 14,000 rpm in a centrifuge (Eppendorf) at 4°C for 2 h as described previously (Holmes et al ...
-
bioRxiv - Paleontology 2020Quote: ... 5 mg of bone powder was transferred to a 2 mL Eppendorf® tube (Eppendorf; Westbury, NY, USA) and 1 mL of demineralizing solution (5% trifluoroacetic acid (TFA ...
-
bioRxiv - Neuroscience 2022Quote: ... 1-2 fecal pellets were collected from each mouse by voluntary defecation into a sterile microtube (Eppendorf, Germany) and fecal samples were immediately placed on dry ice ...
-
bioRxiv - Cancer Biology 2023Quote: ... and then gently shaken at 300 RPM for 2 hr at 37°C using a Thermomixer C (Eppendorf). Sample pH was then adjusted to 4 with 15 μL of 20% formic acid before loading onto conditioned SPE Bond-Elute pH columns (Agilent ...
-
bioRxiv - Microbiology 2023Quote: ... two milliliters of the fermentation broth containing the suspended mucin beads were sampled to 2-ml tubes (Eppendorf). The serum bottles were opened and closed carefully each time to avoid contamination in the anaerobic workstation ...
-
bioRxiv - Microbiology 2023Quote: ... 2 μM final test concentration) were mixed (reaction mix) and transferred into a white 96 well plate (Eppendorf Twin-Tec ...
-
bioRxiv - Cell Biology 2024Quote: ... 2 dpf zebrafish larvae close to the heart by using an electronically regulated air-pressure microinjector (FemtoJet, Eppendorf). After injection ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were plated on 24-well cell imaging plates (black plate with treated glass bottom, Eppendorf) and treated with siRNAs and 100nM nocodazole accordingly ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were plated on 24-well cell imaging plates (black plate with treated glass bottom, Eppendorf) and treated with drugs and/or siRNAs accordingly ...
-
bioRxiv - Developmental Biology 2021Quote: ... cell imaging dish Catalog #0030740009 (Eppendorf) and submerged in imaging solution (80% egg white and 20% 1.2% UltraPure LMP Agarose Catalog #16500100 (Invitrogen ...
-
bioRxiv - Microbiology 2019Quote: ... cells were harvested by centrifugation (Eppendorf) at 4°C for 10 min at 7000 g and then washed twice with and re-suspended in the relevant suspending liquid (NaCl 150mM or 1.5 mM) ...
-
bioRxiv - Microbiology 2023Quote: ... on Cell Imaging Plates (Eppendorf, Germany). Immediately before the infection or 1 ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein solution was then kept on ice whilst the absorbance at 280nm was measured using a BioPhotometer® D30 (Eppendorf) spectrophotometer ...
-
bioRxiv - Genomics 2022Quote: ... they froze and crushed a single, adult female spider with a pellet pestle (Fisher, #12-141-364) in a Protein LoBind tube (Eppendorf, #022431081) containing 200 uL of Buffer CT ...
-
bioRxiv - Genomics 2022Quote: ... We flash froze adult spiders in liquid N2 and crushed them with a pellet pestle (Fisher, 12-141-364) in a Protein LoBind tube (Eppendorf, 022431081) containing 220 uL of Buffer ATL ...
-
bioRxiv - Neuroscience 2021Quote: ... CSF samples were collected by pre-pulled glass capillary tube from the cisterna magna and then transferred to 0.5mL Protein LoBind Eppendorf tubes (Eppendorf Cat #022431064) for centrifugation at 12,700 rpm for 7 minutes at 4°C ...
-
bioRxiv - Genomics 2022Quote: ... the PCR products for apoLp and green fluorescent protein (GFP) were amplified from cDNA in a Mastercycler Nexus instrument (Eppendorf, Hamburg) with a 2-min initial denaturation at 94 °C followed by 30 cycles of 15 sec at 94 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... and incubated (gentle agitation, 20 minutes, 4°C) in EDTA solution (20 mM EDTA-PBS, Ca/Mg-free) in LoBind Protein tubes (Eppendorf 0030122216). The specimen was shaken ...
-
bioRxiv - Microbiology 2024Quote: ... The traps were washed three times with 150 µL S-Trap buffer and placed in clean 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind). Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade ...
-
bioRxiv - Neuroscience 2021Quote: ... The extraction step incorporated a 2-fold concentration of saliva samples using a vacuum concentrator (Concentrator plus, Eppendorf, Germany) resulting in a detection sensitivity of 3pg/ml ...
-
bioRxiv - Genomics 2020Quote: ... cultures were diluted to OD ∼ 0.02 in 1.4 mL of LB and were further grown for 2 h at 37°C on a Thermomixer (Eppendorf) with shaking (700 rpm) ...
-
bioRxiv - Biochemistry 2021Quote: ... β-chitin aliquots from the culture flasks were transferred to 2 mL Safe-Lock Eppendorf tubes (Eppendorf, Hamburg, Germany) and boiled directly for 5 min in 30 µL NuPAGE LDS sample buffer and NuPAGE sample reducing agent (Invitrogen™ ...