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Citations for Eppendorf :
351 - 399 of 399 citations for Human Gap Junction Alpha 8 Protein CX50 GJA8 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... We flash froze adult spiders in liquid N2 and crushed them with a pellet pestle (Fisher, 12-141-364) in a Protein LoBind tube (Eppendorf, 022431081) containing 220 uL of Buffer ATL ...
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bioRxiv - Neuroscience 2021Quote: ... CSF samples were collected by pre-pulled glass capillary tube from the cisterna magna and then transferred to 0.5mL Protein LoBind Eppendorf tubes (Eppendorf Cat #022431064) for centrifugation at 12,700 rpm for 7 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in 400 μl 1XHB buffer and transferred to a 2 ml Eppendorf Protein Lo-Bind tube (Eppendorf, UK). 400 μl of 50% Iodixanol solution (Opti-Prep Density Gradient Medium ...
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bioRxiv - Genomics 2022Quote: ... the PCR products for apoLp and green fluorescent protein (GFP) were amplified from cDNA in a Mastercycler Nexus instrument (Eppendorf, Hamburg) with a 2-min initial denaturation at 94 °C followed by 30 cycles of 15 sec at 94 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated (gentle agitation, 20 minutes, 4°C) in EDTA solution (20 mM EDTA-PBS, Ca/Mg-free) in LoBind Protein tubes (Eppendorf 0030122216). The specimen was shaken ...
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bioRxiv - Microbiology 2024Quote: ... The traps were washed three times with 150 µL S-Trap buffer and placed in clean 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind). Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade ...
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bioRxiv - Bioengineering 2024Quote: ... BMP-2 solution was added to FN coated polymers and after 2 h incubation solution was aspirated and collected in Protein LoBind Tubes (Eppendorf™). Enzyme-linked immunosorbent assays (ELISA ...
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bioRxiv - Pathology 2024Quote: ... BBB organoids from several wells of GRI3D 96-well plate (MSPPGRI3D-96P-S) were collected in a Protein LoBind® tube (0030108116, Eppendorf) by flushing them out with 0.1% Triton X-100 (X100PC ...
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bioRxiv - Systems Biology 2024Quote: 20 mL of culture from each of 3 experimental replicates (n = 3) was collected in Protein LoBind tubes (Eppendorf, Hamburg, Germany) by centrifugation at 1650 xg for 10 min ...
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bioRxiv - Biophysics 2021Quote: ... Experiments on the seconds to minutes time scale were performed at room temperature in 96-well Protein LoBind plates (Eppendorf, Hamburg, Germany).
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bioRxiv - Genomics 2020Quote: ... 3X in Ribohyb buffer and resuspended in a final volume of 10 ul Ribohyb buffer in a non-stick tube (Eppendorf Protein LoBind). The 10 µl hybridization reactions were added to 10 µl of resuspended beads for a binding reaction volume of 20 µl ...
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bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
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bioRxiv - Neuroscience 2020Quote: Aggregate enrichment was performed by centrifugation of a total of 150 μg protein at 20’800 g in a tabletop centrifuge (Eppendorf 5417r, Hamburg, Germany) for 1 hour at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples of N-NTD and N-NTD-SR at 20 µM were prepared in low-binding microtubes (Eppendorf® LoBind) containing 20 mM Tris-HCl buffer (pH 7.5 ...
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bioRxiv - Systems Biology 2022Quote: ... each SCN was cut into 3 pieces with a scalpel on a glass slide and transferred to a protein LoBind tube (Eppendorf, Hamburg, Germany) prefilled with 250 µL lysis buffer (100 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... in 25 mM AB) containing 50 µg of lysate protein and shaken overnight in a thermomixer (Eppendorf; 1400 rpm at 37 °C).
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bioRxiv - Cell Biology 2022Quote: ... The crude extract was centrifuged for 15 min at 13,000 rpm at 4 °C to remove any tissue debris and the clear supernatant was transferred to a fresh Protein Low-Bind tube (Eppendorf, Hamburg, Germany). The total protein content of the samples was estimated using Pierce BCA protein assay kit from Thermo Scientific (Rockford ...
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bioRxiv - Genomics 2023Quote: ... approximately 1.0-2.0 x 105 GFP+ neurons per pup were collected by FACS into a 1.5 mL Protein LoBind Tube (Eppendorf, cat. no. #Z666505).
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bioRxiv - Neuroscience 2024Quote: ... The top phase was carefully removed and then the bottom phase was carefully transferred to a new tube without removing protein from the intermediate layer and dried without heat in a vacuum centrifuge (Vacufuge Plus, Eppendorf, Enfield, CT) and stored at -80°C until MS analysis.
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bioRxiv - Plant Biology 2024Quote: ... homogenized by vortexing and incubated at room temperature with Protein Assay Dye Reagent for 5 minutes before absorbance reading at 595 nm (Eppendorf Biophotometer D30).
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bioRxiv - Genomics 2024Quote: ... and Cas9 protein (100 ng/μl) were injected into individual eggs using a FemtoJet and InjectMan NI 2 microinjection system (Eppendorf, Hamburg, Germany). The microinjection process was completed within 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Digestion was stopped using 1% trifluoroacetic acid (TFA) and the resulting peptides were collected using a magnetic rack and transferred into protein Lo-Bind Eppendorf tubes (Eppendorf, Hamburg, Germany). The peptides were stored at - 80°C and were dried using a vacuum concentrator (CentriVap® Concentrator ...
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bioRxiv - Biochemistry 2024Quote: ... four times (1600 μL) ice-cold 80% acetone was added to 400 μL of urine in 2 ml protein Lo-Bind tubes (Eppendorf, Hamburg, Germany). After incubating at −20 °C for 1hr ...
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bioRxiv - Genetics 2024Quote: ... and Cas9 protein (200 ng/μl) were injected into individual eggs using a FemtoJet and InjectMan NI 2 microinjection system (Eppendorf, Hamburg, Germany). The whole process was completed in two hours ...
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bioRxiv - Systems Biology 2024Quote: ... placed in a 2mL Protein LoBind® Eppendorf tube and centrifuged at 1000g for 2 minutes at RT (Microcentrifuge 5415, Eppendorf, Hamburg, Germany).
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bioRxiv - Neuroscience 2024Quote: ... Binding of the proteins to the beads was induced with 120 µl of absolute ethanol (cat #459836) and mixed using a Thermomixer (Eppendorf, Macquarie Park, Australia) at 1600 rpm for 8 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... The molecular weight cut-off columns were rinsed by addition of equal volumes of Milli-Q water and the proteins were dried with a speed-vac (Eppendorf, Vacufuge Concentrator, Stevenage, UK).
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bioRxiv - Biochemistry 2023Quote: ... Polypropylene standard safe-lock microcentrifuge vials and corresponding low protein binding vials of 1.5 mL size from three manufacturers were used: Manufacturer A (Eppendorf Safe-Lock Tubes no. 0030120086 and Protein LoBind® Tubes no. 0030108116, Eppendorf SE, Hamburg, Germany), Manufacturer B (Pierce™ Microcentrifuge Tubes no ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was removed and cells were resuspended in cold PBS at 20 million cells/mL and then distributed into 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind tubes (Z666505-100EA) at 1 mL each ...
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bioRxiv - Neuroscience 2024Quote: ... Serial dilutions for a given standard were all performed using the same pipette tip and were conducted in Protein LoBind® Tubes (Eppendorf, Hamburg, Germany; #0030108434) to minimize loss of α-synuclein between dilutions through adherence to pipette tips and tube walls ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue punches were checked to ensure they did not contain noticeable quantities of white matter and then inserted into DNAase and RNAase-free 1.5 mm LoBind (Eppendorf Protein LoBind Tube, Cat #22431102) microcentrifuge tubes that were inserted into pulverized dry ice ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were pelleted by centrifugation at 15.000 x g (10 min, 4°C in cold cabinet, Eppendorf centrifuge #5418, rotor FA-45-18-11). After allowing the pellet to air dry ...
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bioRxiv - Molecular Biology 2021Quote: ... Flowrate was adjusted at 2 ml/min to collect peptides in 1 ml fractions in low-protein binding Eppendorf tubes (Eppendorf LoBind tubes, cat. no. EPPE0030108.116). The bound complexes were separated from β2 microglobulin and heavy chain using increasing concentration of buffer B ...
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bioRxiv - Neuroscience 2021Quote: ... were incubated with ten-fold molar excess S-XL6 for 4 hours at 37 °C in protein low bind Eppendorf tubes using Eppendorf Thermomixer at 350 rpm (Eppendorf North America, Enfield, CT, USA). After incubation ...
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bioRxiv - Neuroscience 2024Quote: ... The adjacent 3-5 sections of 100 micron tissue sections were collected in a pre-chilled 2mL microcentrifuge tube (Eppendorf Protein LoBind Tube, Cat #22431102) to total a volume of approximately 40 mg for each donor ...
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bioRxiv - Neuroscience 2024Quote: ... 100 µm sections of the BLA were collected in pre-chilled 2 mL DNAase and RNAase-free microcentrifuge tubes (Eppendorf Protein LoBind Tube, Cat #22431102) for a total of 70-100 mg of tissue and stored at -80°C until nuclear isolation.
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bioRxiv - Biochemistry 2024Quote: ... clean oocytes collected from the above steps were transferred into 3 μL of master mix at the bottom of 384-well plates (Eppendorf, Protein LoBind, order No. 0030624300) or autosampler vials (Waters ...
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bioRxiv - Microbiology 2021Quote: ... following the standard protocol in this kit and liquid handling in Eppendorf epMotion 5075 (Eppendorf North America). The extracted RNA was transcribed into cDNA according to a standard protocol using iScript™ cDNA Synthesis Kit (BIO-RAD ...
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bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
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bioRxiv - Genomics 2020Quote: ... samples were processed according to the Qiagen PowerMag Microbiome DNA/RNA Isolation Kit protocols on an epMotion 5073 liquid handling robot (Eppendorf). Extracted DNA was quantified using the QIAxpert A260 dsDNA system (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... 10 µl of the samples or blanks were pipetted on the 96 well-plate based kit containing calibrators and internal standards using an automated liquid handling station (epMotion 5075, Eppendorf) and subsequently dried under a nitrogen stream using a positive pressure manifold (Waters) ...
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bioRxiv - Immunology 2023Quote: ... The reagents were mixed as per the QuikChange II XL Site-Directed Mutagenesis Kit instructions and loaded into a Mastercycler pro S (Eppendorf) with the settings recommended by the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 µl of the samples or blanks were pipetted on the 96 well-plate-based kit containing calibrators and internal standards using an automated liquid handling station (epMotion 5075, Eppendorf) and subsequently dried under a nitrogen stream using a positive pressure manifold (Waters) ...
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bioRxiv - Microbiology 2024Quote: Sample were retrotranscribed in cDNA using 1 µL of RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
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bioRxiv - Microbiology 2024Quote: Samples were retrotranscribed in cDNA using 1 µL of extracted RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from this homogenate using the NucleoMag® Plant kit (Macherey Nagel, Düren, Germany) on an epMotion 5075 robot according to the manufacturer’s instructions (Eppendorf, Hamburg, Germany). The quality ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GoScript Reverse Transcriptase) provided by the kit was added to start the reverse transcription reaction in the thermocycler (Eppendorf Flexide Mastercycler Nexus) for 5 min at 25°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The homogenate was used to isolate DNA using the NucleoMag® Plant kit (Macherey Nagel, Düren, Germany) on an epMotion 5075 robot according to the manufacturers’ instructions (Eppendorf, Hamburg, Germany). DNA quality ...
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bioRxiv - Molecular Biology 2023Quote: ... we favoured magnetic beads to perform DNA extraction and lysates were processed for DNA extraction using the Macherey-Nagel™ NucleoMag® Tissue kit on an epMotion® 5075vt (Eppendorf, Hamburg – Germany). Volumes on the first binding step were adjusted to the starting volume of lysis buffer accordingly ...