Labshake search
Citations for Eppendorf :
301 - 350 of 632 citations for 8 CYANO IMIDAZO 1 2 A PYRIDINE 2 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... a whole eye from wild type and mutant adult zebrafish or 100 zebrafish mutant larvae were transferred to 2 ml Eppendorf microcentrifuge tubes (Eppendorf, Hamburg, Germany). 200 μl of 2 M hydroxylamine (pH 8 ...
-
bioRxiv - Microbiology 2019Quote: ... were arranged on double-sided sticky tape using a paintbrush and microinjected at the posterior pole of each egg with wAlbB (2×108 bacteria/ml) using a FemtoJet microinjector system (Eppendorf, NSW, Australia). The microinjected eggs were then placed on tissue paper on the surface of artificial manure pats to hatch ...
-
bioRxiv - Immunology 2022Quote: ... Purified IgG was digested into F(ab’)2 with 200 μg of IdeS per 10 mg of IgG for 6 hr on a thermal mixer (Eppendorf ThermoMixer C) at 37°C ...
-
bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf, Hamburg, DE), to constitute 1 million cells per flow sample.
-
ST6GAL1-mediated heterogeneity in sialic acid levels potentiates invasion of breast cancer epitheliabioRxiv - Cancer Biology 2020Quote: 15,000 cells were seeded in 8-well chamber cover glass (Eppendorf, 0030742036). After 24 h ...
-
bioRxiv - Bioengineering 2020Quote: ... Single colonies were grown overnight in 1 mL of SC or SC-ura medium supplemented with 2% glucose in individual wells of the 24-well microtiter plates under constant blue light in an orbital shaker (Eppendorf, New Brunswick, USA) at 30°C and at 200 rpm agitation ...
-
bioRxiv - Systems Biology 2021Quote: ... Cells were resuspended and 50 μl of each pre-culture was used to inoculate 1.5 mL of fresh YPD media (corresponding to an optical density of 0.5 at 600 nm) in 96-deep-well plates (LoBind®, 2 mL, cat no. 0030504305, Eppendorf AG, Hamburg, Germany). Plates were covered with an air permeable membrane and incubated while shaking at 300 rpm and 30 °C for 6 hours ...
-
bioRxiv - Developmental Biology 2019Quote: ... The embryos were deyolked in ice cold ringer’s solution using micropipette tip and spun at 100g for 2 minutes at cooled table top centrifuge at 40C (Eppendorf® centrifuge 5418R). The supernatant was discarded and the embryo bodies were trypsinized using TrypLE Express (Thermoscientific® ...
-
bioRxiv - Biophysics 2020Quote: ... Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
-
bioRxiv - Cell Biology 2021Quote: ... of the same volume as the RIPA buffer at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany). The digested sample was labelled as the RIPA-insoluble fraction (Ins) ...
-
bioRxiv - Microbiology 2023Quote: ... algal cells with the attached bacteria in the treatment with the species consortium were also collected by a pipette from the formaldehyde-fixed suspended sample and transferred into 2 ml safe-lock tubes (Eppendorf, Wesseling-Berzdorf, Germany). To avoid an excessive fixation ...
-
bioRxiv - Systems Biology 2024Quote: ... placed in a 2mL Protein LoBind® Eppendorf tube and centrifuged at 1000g for 2 minutes at RT (Microcentrifuge 5415, Eppendorf, Hamburg, Germany).
-
bioRxiv - Developmental Biology 2024Quote: ... One- or 2-cell stage embryos were injected with approximately 20 nl of the injection mixture (manually or using an Eppendorf FemtoJet 4x microinjector) and maintained at 20°C until the 64-cell stage ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... 8 animals were kept at each concentration in a 48-well plate (Eppendorf) and imaged in brightfield at 4x with an Invitrogen EVOS Fl Auto 2 (Thermo-Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... and overlayed with Matrigel including medium in 8 well chamber coverglass (0030742036; Eppendorf) as previously described (Debnath et al. ...
-
bioRxiv - Microbiology 2021Quote: ... were used in combination with an 8-channel 50-µL volume adaptor (Eppendorf). Each deep-well culture was used to inoculate 2 96-well flat-bottom plates as replicates for the growth curve measurements ...
-
bioRxiv - Developmental Biology 2021Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using the FemtoJet 4i (Eppendorf, Hamburg, Germany; Cat. No. 5252000013) micro-injector ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were spun down (5 min, 800 g•, 2 °C, minimal acceleration and break, Eppendorf 5810 R with swing-×bucket rotor A-4-44). The medium was discarded and the cells were dissociated in 15 mL of ice cold ACK solution (0.15 M NH4Cl ...
-
bioRxiv - Cell Biology 2020Quote: ... This solution was centrifuged at 3000g for 2 min and the supernatant was collected and concentrated in a vacuum concentrator in vacuum-alcoholic (V-AL) mode (Eppendorf plus, Eppendorf, Hamburg, Germany). The concentrated crystals were resuspended in 100μl of 1 X PBS and Nuclease Free Water (NFW) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were loaded into a microinjection needle and 50 nL of the ZMEL cell suspension was transplanted into the hindbrain ventricle of anesthetized 2 dpf zebrafish larvae by using an oil-controlled microinjection rig (Eppendorf CellTram 4r Oil, #5196000030) with a Narishige arm ...
-
bioRxiv - Bioengineering 2023Quote: ... Transwells® were then incubated at 37 °C and 25 RPM for 2 hrs in a New Brunswich™ Innova® 40 shaker (Eppendorf, Enfield, CT). Samples were collected every hour from the basolateral side ...
-
bioRxiv - Genomics 2024Quote: ... 2 µL sheared DNA was used to perform overnight pulse-field gel electrophoresis while the remaining sheared DNA was stored in a 2 mL DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C overnight ...
-
bioRxiv - Microbiology 2023Quote: ... Each flask was aeriated with 2% v/v CO2 enriched air which was controlled by a DASGIP® MX module (Eppendorf AG, Hamburg, Germany). The microalgae starting with an OD750 of 0.1 were cultivated in modified Johnson medium at a pH of 7.5 [49] with 1 M NaCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... complete amino acid supplementation) overnight in 96-well flat bottom plates (Eppendorf), diluted into fresh medium with a dilution factor of 200 ...
-
bioRxiv - Biochemistry 2020Quote: ... polar phase were transferred into a new 2 mL reaction tube and dried in a vacuum concentrator (Eppendorf, Concentrator plus, mode: V-AQ, 30 °C) for approximately 4.5 h ...
-
bioRxiv - Plant Biology 2023Quote: ... Total RNA samples were collected in 2.0 ml nucleic acid LoBind tubes (Eppendorf) and stored at −80 °C ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μl of the lipid-containing lower organic phase was spotted on a MALDI target plate (Prespotted AnchorChip 96 Set for Proteomics II; Bruker Daltonics/Eppendorf; washed peptide-free with 2-propanol) pre-spotted with 1 µl of 9-aminoacridine (10 mg/ml dissolved in acetone:water 9:1 (v/v) ...
-
bioRxiv - Biophysics 2020Quote: ... with three ssDNA overhang strands on a bottom partially complementary to the sequence on the magnetic beads (mag2, Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
-
bioRxiv - Microbiology 2022Quote: ... 10 mM HEPES and seeded on confluent HFF monolayers in 8-well chamber slides (Eppendorf) at a density of 5×105 tachyzoites/well ...
-
bioRxiv - Biochemistry 2022Quote: ... and 8% CO2 in a New Brunswick S41i CO2 Incubator (New Brunswick Eppendorf, Hamburg, Germany) for 4 days and passaged twice to 0.5×106 cell/mL into a 250 mL shake flask and then into a 500 mL shake flask ...
-
bioRxiv - Biophysics 2021Quote: ... in particular to adjust reactions volumes to the 8- or 12-channel pipettes (Eppendorf, Hamburg, Germany), repetitive pipette HandyStep (Brand ...
-
bioRxiv - Molecular Biology 2019Quote: ... Phenol-chloroform (50:50) was added and cells were vortexed for 8 min (Eppendorf mixer 5432). The DNA was ethanol precipitated and resuspended in 40-50 µL TE (10 mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... placed on an orbital shaker platform rotating at 125 rpm at 37 °C with 8 % CO2 (Eppendorf). On the day of transfection ...
-
bioRxiv - Biochemistry 2022Quote: Wholemeal flour samples were weighed (~8 mg) and transferred into a deep well plate (96/1000 μL, Eppendorf), each well containing 20 μL of DMSO and a 3 mm glass ball to improve mixing ...
-
bioRxiv - Biochemistry 2023Quote: ... 380 μL samples of 8 μM aSyn solutions were incubated in Protein LoBind polypropylene tubes (Eppendorf, ref.0030108.116) at 37 °C under quiescent conditions over 10 days in the absence and presence of a cylindrical 10 mm x 3mm Teflon stirring bar (Merck ...
-
bioRxiv - Cell Biology 2022Quote: ... Eluted lysates in 60% acetonitrile/0.1% formic acid were dried by vacuum centrifugation (Eppendorf; Concentrator Plus) at 45°C.
-
bioRxiv - Biochemistry 2023Quote: ... in 0.3% acetic acid was added to a 96-well plate (CAT# 0030128664, Eppendorf, Hamburg, Germany), sealed with plate seal (CAT# 5010-21951 ...
-
bioRxiv - Immunology 2021Quote: ... Resulting cDNA was diluted 1:8 and subjected to endpoint RT-PCR using Taq DNA Polymerase (GeneDirex Inc., Taoyuan, Taiwan) in a Mastercycler Nexus (Eppendorf) or Bio-Rad T100 thermocycler ...
-
bioRxiv - Microbiology 2019Quote: ... Two hundred microliters of supernatant were transferred to tubes containing 8 µL neutralization solution (15% NH4HCO3) and dried in a Vacufuge concentrator (Eppendorf) for 30 to 45 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... The citrated blood was mixed by gentle inversion and 8 mL was transferred to a 15 mL LoBind conical tube (Eppendorf), then placed in a heated water bath set to 37 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... with 200 μL of 3.2 mm round beads at for 10 min at setting 8 in 1.8 mL microcentrifuge vials (Eppendorf Safe-Lock). Homogenates were transferred to a new microcentrifuge vial and briefly placed on ice for phase separation before centrifugation at 14,000 × g for 15 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... one of the aggregated cultures was transferred to a reagent reservoir (VistaLab Technologies) and mixed 50 times with an 8-channel micropipette (Eppendorf Xplorer ...
-
bioRxiv - Neuroscience 2024Quote: ... samples were layered on top of the 105 µl cushion and spun at 10,000 g for 20 min at 4°C in a swing out rotor (A-8-11 swing bucket rotor, Eppendorf) to isolate CpxII bound to trans SNARE complexes (SNAREpins ...
-
bioRxiv - Biochemistry 2022Quote: ... After 5 min incubation at room temperature (RT) the sample was centrifuged (8 min, RT, 180 x g, Eppendorf centrifuge 5810R) to separate proteoliposomes from the non-incorporated protein and detergent ...
-
bioRxiv - Cancer Biology 2019Quote: ... Pellet of clusters was resuspended in 50 μL of neutralized collagen and seeded in 8-well chambered cover glass (Eppendorf 0030742036) and supplemented with defined medium ...
-
bioRxiv - Systems Biology 2019Quote: ... and the bacterial cells were harvested by centrifugation (swing-out rotor A-4-44, Eppendorf; 3220 g, 8 min, 30°C). The cell pellet was resuspended in the mineral medium and centrifuged again ...
-
bioRxiv - Microbiology 2022Quote: ... 10 μL was administered to each treatment group (of 8 larvae) via oral inoculation between the mandibles using long gel loading tips (Eppendorf, UK). At each desired timepoint ...
-
bioRxiv - Cell Biology 2019Quote: ... Digested peptides were extracted from gels using 50% ACN solution with 2.5% formic acid (FA) and concentrated in speedVac concentrator (Eppendorf). The aliquot (1/10 ...
-
bioRxiv - Cell Biology 2019Quote: ... Digested peptides were extracted from gels using 50% ACN solution with 2.5% formic acid (FA) and concentrated in speedVac concentrator (Eppendorf). The aliquot (1/10 ...
-
bioRxiv - Systems Biology 2020Quote: ... Samples were eluted with 60 μL of buffer B (80% ACN, 0.1% formic acid in H20) and reduced in a Vacufuge plus (Eppendorf) to a final volume of 3 μL ...