Labshake search
Citations for Eppendorf :
301 - 350 of 727 citations for 2 4 Dimethyl 4' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Biomass samples were collected in mid-exponential phase by centrifuging (3214 x g; Eppendorf 5810 R, S-4-104 rotor) 50 mL of culture for 15 minutes at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... was added without disturbing the pellet and nuclei were pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). The supernatant was removed without disturbing the pellet and 7-10 µl of 1x Nuclei Buffer (10x Genomics ...
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bioRxiv - Genomics 2024Quote: ... and incubated on ice for 1 minute before pelleting with a swinging-bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). The supernatant was gently removed and ∼50 µl were left behind to increase nuclei recovery ...
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bioRxiv - Microbiology 2024Quote: ... which were centrifuged at 3220 x g for 30 min at room temperature (Eppendorf A-4-62 Swing Bucket Rotor). The interface layer containing microbial cells was recovered ...
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bioRxiv - Cell Biology 2024Quote: ... the cell lysate was collected and agitated for 30 min at 800 rpm at 4 °C in a Thermomixer (Eppendorf). Afterwards ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were transferred to SMART Digest Trypsin digest resin in PCR strips and incubated 4 h at 70°C and 1400 rpm mixing (Thermomixer C with ThermoTop, Eppendorf). Cysteines were 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... After The upper lipophilic phases and lower hydrophilic phases were separated by high-speed centrifugation (12,700 rpm, 5 min at 4 °C, Centrifuge 5430R, Eppendorf, Germany). Next ...
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bioRxiv - Cancer Biology 2024Quote: ... extracts were centrifuged for 20 minutes at 18,000 x g (4°C) using a Eppendorf™ Centrifuge 5430 R (Eppendorf), to pellet out cellular debris ...
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: The DNA scaffold was self-assembled by incubating 5 DNA oligonucleotides following a temperature gradient from 95 °C to 4 °C for 6 h in a thermocycler (Mastercycler® nexus X2, Eppendorf) in Tris-EDTA buffer (TE buffer ...
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bioRxiv - Microbiology 2021Quote: ... an aliquot of the culture was removed for CFU determination by plate counting and the remainder was centrifuged at 4°C and 3,100 rpm (Eppendorf Centrifuge 5415R) for 10 min to generate cell-free supernatants ...
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bioRxiv - Neuroscience 2020Quote: ... 200 mM solution in water) was then added to the samples and incubated for 4 hours at 21°C in a ThermoMixer (Eppendorf AG). 4 µL of 1M IAA (iodoacetamide in water ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were incubated on ice for 30 min before the demembraned cilia were centrifuged at 16,000g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415D). The supernatant was discarded ...
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bioRxiv - Microbiology 2021Quote: ... Serum was separated from blood by centrifugation at 8,000 RPM for 30 minutes at 4 °C in an Eppendorf 5424R centrifuge (Eppendorf, Enfield, CT). Serum was heat inactivated by incubating at 56 °C for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: 96 well plates were coated with immunized vaccine antigen and incubated for two hours at 25 °C (4 μg/mL, in 1xPBS, 50 μL/well) under constant shaking (300 rpm) on a MixMate thermomixer (Eppendorf, USA). ACE2-hFc protein coating was used as a control for antigen immobilization ...
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bioRxiv - Biophysics 2020Quote: 96 well plates were coated with vaccine antigen and incubated overnight at 25 °C (4 μg/mL in 1x PBS, 50 μl/well) under constant shaking (300 rpm) on a MixMate thermomixer (Eppendorf, USA). Ovalbumin (4 μg/mL in 1x PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... Solutions were then spun down at 13,000 RPM for 30 minutes at 4°C in a benchtop micro-centrifuge (Eppendorf Centrifuge 5424) to remove cells and other solid debris ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were centrifuged at 14k rpm for 15 minutes at 4°C and the aqueous phase was collected into clean 1.5 mL Eppendorf tubes (Eppendorf, Hauppauge, NY). 500 µL of ice-cold isopropanol was added to each tube ...
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bioRxiv - Microbiology 2020Quote: ... The cultures were grown in BHI broth for 24 h and were centrifuged at 3200 × g for 20 min at 4°C (Eppendorf, 5424R). The cell pellets were transported on ice for proteome analysis at The Mass Spectrometry Centre ...
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bioRxiv - Bioengineering 2020Quote: ... Cotton swabs containing simulated forensic samples were placed in 500 μL of 1X PBS and mixed at 4°C in a Thermomixer (Eppendorf, Germany) set at 1,000 rpm for approximately an hour ...
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bioRxiv - Bioengineering 2020Quote: ... and microbubbles were isolated by 4 centrifugation wash cycles at 40 relative centrifugation force for 1 min (Eppendorf 5804, Hamburg, Germany), where the infranatant was discarded and the microbubble concentrate was saved and resuspended.
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was used to fractionate the cell lysate (at 4 °C for 30 min at 4,416 g, Eppendorf, Centrifuge 5804 R) and at 4 °C for 1 hour for ultracentrifugation (70,658 g ...
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bioRxiv - Neuroscience 2023Quote: ... The cell culture supernatant was collected and pre-cleared from cells and larger particles by centrifugation at 400 × g at 4 °C for 10 min (Eppendorf 5810R) and 10,000 × g at 4 °C for 30 min (Eppendorf 5910R) ...
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bioRxiv - Biophysics 2023Quote: ... Lysate and cell fragments were separated by centrifugation at 4 °C for 20 min at 12,000 rpm (Centrifuge 5418 R, Eppendorf, Hamburg, Germany). Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: The filled needle is positioned on a micromanipulator (Narishige MMO-4) and connected to a positive pressure pump (Eppendorf FemtoJet 4i). Control embryos were injected with Cas9 protein and Lap2b-GFP mRNA only.
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bioRxiv - Neuroscience 2022Quote: ... Blood samples were collected on ice and centrifuged within 1h at 4°C for 10min at 3000 RPM (Eppendorf, Centrifuge 5804R). Separated plasma was stored at -80°C until the protein assay was initiated.
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bioRxiv - Neuroscience 2022Quote: ... and incubated (gentle agitation, 20 minutes, 4°C) in EDTA solution (20 mM EDTA-PBS, Ca/Mg-free) in LoBind Protein tubes (Eppendorf 0030122216). The specimen was shaken ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were washed with cold 70% ethanol and centrifuged at 19,000 × g for 5 min at 4°C and air dried for 15 min in the Centrifugal Vacuum Concentrator 5301 (Eppendorf, USA). The quality of RNA was assessed using a NanoDrop (ND-1000 ...
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bioRxiv - Microbiology 2024Quote: ... Cells grown under photoautotrophic and dark heterotrophic conditions were collected using centrifugation (6,000 rpm, 10 min, 4°C; R10A2 rotor, Himac CR21F, Eppendorf Himac Technologies) and washed with a small amount of PBS ...
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bioRxiv - Microbiology 2024Quote: ... The stationary pre-cultures were then pooled and washed by centrifugation (5 min, 3,220 g and 4 °C) using a 5810 R swing-out centrifuge (Eppendorf, Hamburg, Germany). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... The crude EV fractions were prepared by ultracentrifugation (45,000 rpm, 1 h, 4°C; S50A rotor, Himac Ultracentrifuge CS100GXII; Eppendorf Himac Technologies) of the thus prepared supernatants.
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bioRxiv - Cancer Biology 2022Quote: ... Samples were vigorously vortex-mixed for 3 min and centrifuged at 14,000 rpm for 10 min at 4 °C (Eppendorf 5804 R). The clear supernatant was then transferred to a separate vial ...
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bioRxiv - Neuroscience 2022Quote: ... 3 times with 10” ON and 30” OFF intervals on 20% power on ice and cleared by centrifugation at 21,000 rpm for 20min at 4°C (Eppendorf 5424R Centrifuge). 50µg of supernatant was mixed with NuPAGE LDS Sample Buffer (Thermo Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... annealing at 51 °C for 30 s and extension 72 °C for 30 s for 25 cycles by final extension 72 °C for 3 min and reaction hold at 4 °C in a thermocycler (Gradient thermocycler® Eppendorf). Amplified gene products were sequenced using the Sanger sequencing method ...
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bioRxiv - Biochemistry 2023Quote: ... in 5% glucose solution v/v prior to adding 2X solubilization buffer and incubation at 37 °C for 4 hrs under gentle orbital agitation (500 rpm on Eppendorf Thermomixer). The product was then analyzed by SEC as described earlier.
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bioRxiv - Developmental Biology 2022Quote: ... The filled needle is positioned on a micromanipulator (Narishige MMO-4) and connected to a positive pressure pump (Eppendorf FemtoJet 4i). Embryos are placed in FHM drops covered with mineral oil under Leica TL Led microscope ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 1 hour at a temperature of 4°C with shaking at 600 rpm in a Thermomixer comfort (Eppendorf, Germany). Stained cells were washed with ice cold FACS buffer twice before resuspension in FACS buffer for analysis ...
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bioRxiv - Cell Biology 2024Quote: ... the resuspended cilia were centrifuged at 16000 × g for 10 minutes at 4°C in a microfuge (Eppendorf, Centrifuge 5415 D), and the supernatant was removed ...
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bioRxiv - Cell Biology 2024Quote: ... The protoplasts were collected by centrifugation at 1700 rpm for 1 minute using a swing-bucket rotor (Eppendorf S-4-72), washed with 15 mL of ice-cold solution 3 (26.4 g of ammonium sulfate ...
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bioRxiv - Neuroscience 2024Quote: ... They were then resuspended to a concentration of ∼800-3000 nuclei per uL across 3-4 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512) in NSB ...
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bioRxiv - Microbiology 2024Quote: ... An overnight culture of 1 ml was pelleted down by centrifugation ((10,000 rpm at 4°C for 10 min; Eppendorf centrifuge 5810R) and washed thrice with M9 minimal (M9M ...
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bioRxiv - Physiology 2024Quote: ... plasma samples were thawed from storage at -80°C and centrifuged (5,000 x g, 10 minutes, 4 °C; Eppendorf 5427R with rotor FA-45-48-11; Eppendorf, Enfield, CT). Supernatants were transferred to 96-well plates and stored at -80°C until analysis ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 30 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Immunology 2022Quote: ... 20 μL of mAb solution was added to the cells in the filter wells and the cells were incubated for 1 h at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). After the incubation ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 45 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was spun down at 13600 rpm for 10 minutes at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysate was spun down at 13600 rpm for 10 min at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Immunology 2021Quote: ... The 80 µL aliquot was lyophilised for 4 h at 30 °C with the aid of a vacuum concentrator (Eppendorf Concentrator 5301) attached to a refrigerated condensation trap (Savant) ...
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bioRxiv - Systems Biology 2021Quote: ... Total volume of 20 mL from each culture was transferred to a 50 mL Falcon® filled with ice and was immediately centrifuged at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). Next the supernatant was discarded and the cell pellet was snap frozen into liquid nitrogen and stored at -80 °C ...
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bioRxiv - Systems Biology 2021Quote: For the extraction of total proteome 10 mL of each culture were transferred into ice-cold 15 mL Falcon® tubes which were centrifuged immediately at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). The supernatant from the centrifugation was discarded and the cell pellets were washed once with 1 mL of cold PBS buffer ...
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bioRxiv - Biophysics 2020Quote: ... 200 ml of M9 minimal media were added to 4 mL of pre-inoculum and the cell growth was monitored using Biophotometer D30 (Eppendorf, Hamburg, Germany) until the growth rate was 0.8 (OD at 600 nm) ...