Labshake search
Citations for Eppendorf :
251 - 300 of 301 citations for Rat Heat Shock Protein HSP 90 Beta HSP90AB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... 1 mM β-glycerol phosphate and 2.5 mM Na2H2P2O7).) and cells scraped and transferred into Protein Lo-bind tubes (Eppendorf). Samples were sonicated for 10 cycles (30s on & 40s off ...
-
bioRxiv - Molecular Biology 2021Quote: ... The beads were pelleted (600 x g, 2 min) and the supernatant was transferred to a fresh low protein binding tube (Eppendorf). The beads were washed with 2 × 300 μL HPLC grade water and the washes combined with the original supernatant ...
-
bioRxiv - Synthetic Biology 2022Quote: Excitation and emission spectra of the fluorescent proteins were conducted using a BMG Clariostar Plus microplate reader in sealed plates (Corning, 3370; Eppendorf Masterclear real-time PCR film adhesive ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissues were fixed in 2% paraformaldehyde for 55 minutes at room temperature in 2 mL Protein LoBind tubes (Eppendorf 022431064). Fixative was removed and tissues were washed 4x 10 minutes with 1.5 mL PBS with 0.5% Triton X-100 (PBT) ...
-
bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 3000 x g for 6 min at 4 °C and the conditioned media was transferred to a Protein LoBind tube (Eppendorf) (media fraction) ...
-
bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein concentration in the supernatant was determined according to the Bradford Ultra method (Bradford, 1976) on a Bio-Spectrophotometer (Eppendorf) at 595 nm ...
-
bioRxiv - Cell Biology 2023Quote: ... After a 5-minute incubation the protein concentration was determined through measurement of the OD595 with a photometer (BioPhotometer plus, Eppendorf). Finally ...
-
bioRxiv - Cell Biology 2023Quote: ... The flow rate was 0.2 ml/min and 48 × 100 μl fractions were collected in a low-protein binding 96-deep-well plate (Eppendorf) at 4°C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Samples were then centrifuged at 14,000xg for 10 minutes to pellet insoluble debris and the resultant clarified lysate was transferred to a fresh protein LoBind tube (Eppendorf).
-
bioRxiv - Microbiology 2023Quote: ... The beads were pelleted (600 x g, 3 min) and the supernatant transferred to a fresh low protein binding tube (Eppendorf). The beads were washed with 1 x 200 µL HPLC-grade water and the washes combined with the original supernatant ...
-
bioRxiv - Microbiology 2024Quote: ... A volume of 1 nL containing 500 ng/μL mRNA coding for each of these proteins was injected into one-cell stage embryos using the FemtoJet 4i microinjector (Eppendorf). H2O was used as reference control.
-
bioRxiv - Molecular Biology 2024Quote: ... though the maximum volume of beads + gDNA + binding buffer should not exceed 1.4 ml for a single 1.5 ml Protein LoBind tube (Eppendorf, Cat. 30108442). Multiple tubes can be used and pooled at the restriction enzyme digest step ...
-
bioRxiv - Bioengineering 2024Quote: ... the scaffolds were immersed in 1.5 mL BMP-2 (100 µg/mL) diluted in PBS in a low protein-binding falcon tube (Eppendorf, UK). The bottom of the tube was gently tapped on a surface to remove any bubbles between the pores of the scaffold to allow the solution to completely contact the scaffold ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein solution was then kept on ice whilst the absorbance at 280nm was measured using a BioPhotometer® D30 (Eppendorf) spectrophotometer ...
-
bioRxiv - Neuroscience 2020Quote: ... For bulk PFF production approximately 500μL of 5mg/ml monomeric protein was added to a 2-ml Eppendorf tube and placed on a thermomixer-C (Eppendorf, USA) shaker and incubated at 37°C and 1000RPM for up to 144 hours ...
-
bioRxiv - Genomics 2022Quote: ... they froze and crushed a single, adult female spider with a pellet pestle (Fisher, #12-141-364) in a Protein LoBind tube (Eppendorf, #022431081) containing 200 uL of Buffer CT ...
-
bioRxiv - Genomics 2022Quote: ... We flash froze adult spiders in liquid N2 and crushed them with a pellet pestle (Fisher, 12-141-364) in a Protein LoBind tube (Eppendorf, 022431081) containing 220 uL of Buffer ATL ...
-
bioRxiv - Neuroscience 2021Quote: ... CSF samples were collected by pre-pulled glass capillary tube from the cisterna magna and then transferred to 0.5mL Protein LoBind Eppendorf tubes (Eppendorf Cat #022431064) for centrifugation at 12,700 rpm for 7 minutes at 4°C ...
-
bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in 400 μl 1XHB buffer and transferred to a 2 ml Eppendorf Protein Lo-Bind tube (Eppendorf, UK). 400 μl of 50% Iodixanol solution (Opti-Prep Density Gradient Medium ...
-
bioRxiv - Genomics 2022Quote: ... the PCR products for apoLp and green fluorescent protein (GFP) were amplified from cDNA in a Mastercycler Nexus instrument (Eppendorf, Hamburg) with a 2-min initial denaturation at 94 °C followed by 30 cycles of 15 sec at 94 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... and incubated (gentle agitation, 20 minutes, 4°C) in EDTA solution (20 mM EDTA-PBS, Ca/Mg-free) in LoBind Protein tubes (Eppendorf 0030122216). The specimen was shaken ...
-
bioRxiv - Bioengineering 2024Quote: ... BMP-2 solution was added to FN coated polymers and after 2 h incubation solution was aspirated and collected in Protein LoBind Tubes (Eppendorf™). Enzyme-linked immunosorbent assays (ELISA ...
-
bioRxiv - Microbiology 2024Quote: ... The traps were washed three times with 150 µL S-Trap buffer and placed in clean 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind). Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade ...
-
bioRxiv - Biophysics 2021Quote: ... Experiments on the seconds to minutes time scale were performed at room temperature in 96-well Protein LoBind plates (Eppendorf, Hamburg, Germany).
-
bioRxiv - Genomics 2020Quote: ... 3X in Ribohyb buffer and resuspended in a final volume of 10 ul Ribohyb buffer in a non-stick tube (Eppendorf Protein LoBind). The 10 µl hybridization reactions were added to 10 µl of resuspended beads for a binding reaction volume of 20 µl ...
-
bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
-
bioRxiv - Neuroscience 2020Quote: Aggregate enrichment was performed by centrifugation of a total of 150 μg protein at 20’800 g in a tabletop centrifuge (Eppendorf 5417r, Hamburg, Germany) for 1 hour at 4°C ...
-
bioRxiv - Biophysics 2021Quote: ... The protein samples of N-NTD and N-NTD-SR at 20 µM were prepared in low-binding microtubes (Eppendorf® LoBind) containing 20 mM Tris-HCl buffer (pH 7.5 ...
-
bioRxiv - Systems Biology 2022Quote: ... each SCN was cut into 3 pieces with a scalpel on a glass slide and transferred to a protein LoBind tube (Eppendorf, Hamburg, Germany) prefilled with 250 µL lysis buffer (100 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... in 25 mM AB) containing 50 µg of lysate protein and shaken overnight in a thermomixer (Eppendorf; 1400 rpm at 37 °C).
-
bioRxiv - Cell Biology 2022Quote: ... The crude extract was centrifuged for 15 min at 13,000 rpm at 4 °C to remove any tissue debris and the clear supernatant was transferred to a fresh Protein Low-Bind tube (Eppendorf, Hamburg, Germany). The total protein content of the samples was estimated using Pierce BCA protein assay kit from Thermo Scientific (Rockford ...
-
bioRxiv - Genomics 2023Quote: ... approximately 1.0-2.0 x 105 GFP+ neurons per pup were collected by FACS into a 1.5 mL Protein LoBind Tube (Eppendorf, cat. no. #Z666505).
-
bioRxiv - Biochemistry 2023Quote: ... Samples were thawed and Urea was added to a final concentration of 8 M urea and the protein lysate was transferred to 2mL snap-cap centrifuge tubes (Eppendorf, Hamburg, Germany) with 0.1 mm zirconia beads and bead beat in a Bullet Blender (Next Advance ...
-
bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
-
bioRxiv - Systems Biology 2024Quote: ... placed in a 2mL Protein LoBind® Eppendorf tube and centrifuged at 1000g for 2 minutes at RT (Microcentrifuge 5415, Eppendorf, Hamburg, Germany).
-
bioRxiv - Biochemistry 2021Quote: ... The molecular weight cut-off columns were rinsed by addition of equal volumes of Milli-Q water and the proteins were dried with a speed-vac (Eppendorf, Vacufuge Concentrator, Stevenage, UK).
-
bioRxiv - Biochemistry 2022Quote: ... the supernatant was removed and cells were resuspended in cold PBS at 20 million cells/mL and then distributed into 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind tubes (Z666505-100EA) at 1 mL each ...
-
bioRxiv - Biochemistry 2023Quote: ... Polypropylene standard safe-lock microcentrifuge vials and corresponding low protein binding vials of 1.5 mL size from three manufacturers were used: Manufacturer A (Eppendorf Safe-Lock Tubes no. 0030120086 and Protein LoBind® Tubes no. 0030108116, Eppendorf SE, Hamburg, Germany), Manufacturer B (Pierce™ Microcentrifuge Tubes no ...
-
bioRxiv - Molecular Biology 2021Quote: ... Flowrate was adjusted at 2 ml/min to collect peptides in 1 ml fractions in low-protein binding Eppendorf tubes (Eppendorf LoBind tubes, cat. no. EPPE0030108.116). The bound complexes were separated from β2 microglobulin and heavy chain using increasing concentration of buffer B ...
-
bioRxiv - Neuroscience 2021Quote: ... were incubated with ten-fold molar excess S-XL6 for 4 hours at 37 °C in protein low bind Eppendorf tubes using Eppendorf Thermomixer at 350 rpm (Eppendorf North America, Enfield, CT, USA). After incubation ...
-
bioRxiv - Microbiology 2021Quote: ... following the standard protocol in this kit and liquid handling in Eppendorf epMotion 5075 (Eppendorf North America). The extracted RNA was transcribed into cDNA according to a standard protocol using iScript™ cDNA Synthesis Kit (BIO-RAD ...
-
bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
-
bioRxiv - Genomics 2020Quote: ... samples were processed according to the Qiagen PowerMag Microbiome DNA/RNA Isolation Kit protocols on an epMotion 5073 liquid handling robot (Eppendorf). Extracted DNA was quantified using the QIAxpert A260 dsDNA system (Qiagen ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 µl of the samples or blanks were pipetted on the 96 well-plate based kit containing calibrators and internal standards using an automated liquid handling station (epMotion 5075, Eppendorf) and subsequently dried under a nitrogen stream using a positive pressure manifold (Waters) ...
-
bioRxiv - Immunology 2023Quote: ... The reagents were mixed as per the QuikChange II XL Site-Directed Mutagenesis Kit instructions and loaded into a Mastercycler pro S (Eppendorf) with the settings recommended by the manual ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10 µl of the samples or blanks were pipetted on the 96 well-plate-based kit containing calibrators and internal standards using an automated liquid handling station (epMotion 5075, Eppendorf) and subsequently dried under a nitrogen stream using a positive pressure manifold (Waters) ...
-
bioRxiv - Microbiology 2024Quote: Sample were retrotranscribed in cDNA using 1 µL of RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
-
bioRxiv - Developmental Biology 2019Quote: ... Plasmids used in initial identification screen for cis-regulatory elements used miniprep scale DNA purification (Zymo Research, D4020 or 5 prime/Eppendorf, FastPlasmid kit) and all subsequent experiments used midiprep scale DNA purification (Qiagen ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... GoScript Reverse Transcriptase) provided by the kit was added to start the reverse transcription reaction in the thermocycler (Eppendorf Flexide Mastercycler Nexus) for 5 min at 25°C ...