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Citations for Eppendorf :
201 - 250 of 314 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Reverse transcriptase-polymerase chain reaction (RT-PCR) was carried out on an Eppendorf Mastercycler thermocycler (Eppendorf, Hamburg, Germany) in a single step using the Qiagen OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Systems Biology 2021Quote: ... 19% ddH2O into PCR tubes and finally dried at 60 °C in a SpeedVac centrifuge (Eppendorf, Concentrator plus). Peptides were resuspended in 0.1 % TFA ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μL of the resulting droplet emulsion was transferred to a semi-skirted 96 well PCR plate (Eppendorf), sealed with foil and amplified on a thermal cycler using the following amplification conditions ...
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bioRxiv - Microbiology 2020Quote: The DNA sequence (308 bp) was amplified by PCR in a Mastercycler Pro 6321 (Eppendorf AG, Hamburg, Germany). The components of the PCR reaction mix (50 μl per reaction ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... Antibody binding was conducted in the volume of 50 µl on thermomixer for 96-well PCR plates (Eppendorf) in 4 °C cold room for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the following steps were performed: After having collected single cells (see 2.4) in single nuclease-free 0.2 mL PCR-tubes (Eppendorf AG ...
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bioRxiv - Microbiology 2020Quote: ... the polymerase chain reaction (PCR) was performed on a Mastercycler ep Gradient S thermal cycler (Eppendorf, Hamburg, Germany) using a slow ramp in temperature (0.1 °C s−1 ...
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bioRxiv - Physiology 2019Quote: ... and mitochondrial transcription factor A (TFAM) were quantified by quantitative real-time PCR (Mastercycler® RealPlex2, Eppendorf, Germany), using SYBR Green chemistry (iTaqTM Universal SYBR® Green Supermix ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions were conducted using the following thermocycling parameters in a Mastercycler Pro 6321 (Eppendorf AG, Hamburg, Germany): 94 °C for 3 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Immunocomplexes were eluted in 1% SDS/TE buffer and transferred to Lobind DNA tubes (Eppendorf #13-698-790) at 65°C for 30 minutes and crosslinks were reversed overnight by incubating samples at 65°C ...
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bioRxiv - Systems Biology 2021Quote: ... In brief: crude cell lysates were transferred from source plates to 384 well v-bottom PCR Plates (Eppendorf #951020702) assay plates ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Real-time quantitative PCR was performed using TransStart® Tip Green qPCR SuperMix (AQ141-01) on Realplex2 Mastercycler (Eppendorf).
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bioRxiv - Molecular Biology 2019Quote: ... according to the manufacturer’s instructions and the emulsions (approximately 40 µl) were transferred to a 96-well PCR plate (twin-tec, Eppendorf) and sealed with foil (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... One µl of this cocktail was added to the PCR mixture and placed in a thermocycler (Eppendorf MasterCycler Pro). Thermocycling settings were as follows ...
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bioRxiv - Immunology 2020Quote: ... and RNAse free water for a final volume of 13 µl in 0.2 ml PCR Tube Strips (732-0098, Eppendorf). The reaction tubes were transferred into a C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... PCR amplifications were carried out on an Eppendorf® Mastercycler® Nexus X2 Thermal Cycler (Eppendorf Co., Ltd. Germany) using PrimeSTAR HS DNA polymerase (Takara Biotechnology Co. ...
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bioRxiv - Genomics 2021Quote: ... Positive droplets were gated and sorted out into 15 µL of PCR grade water in a 1.5 ml DNA LoBind tube (Eppendorf).
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription of GEMs was performed in a Eppendorf Mastercycler X50s thermal cycler using 0.2 ml PCR tube strips (E0030124286, Eppendorf): 53 °C for 45 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... using 50 ng of Geneblock as template and 0.5 μM of forward and reverse primer in a PCR Mastercycler (Eppendorf), with initial heating at 98°C for 30s ...
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bioRxiv - Developmental Biology 2020Quote: ... 300-400 female G10011 pupae (at 70-80% pupal development) were injected with dsRNA (2μg /μl) or injection buffer only (control) using a FemtoJet Express (Eppendorf). Injected pupae were placed on fine wheat flour (type 405 ...
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bioRxiv - Genomics 2019Quote: ... Each sample was eluted into 50 ul of Elution Buffer in duplicate into individual DNA LoBind 1.5 ml microcentrifuge tubes (Eppendorf) for storage at 80 °C until use in sequencing.
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bioRxiv - Molecular Biology 2021Quote: ... and re-dissolved in lysis buffer supplemented with 1% v/v SDS at RT in a Thermomixer R (Eppendorf) at 1200 rpm for 1-2 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Dynabeads were separated magnetically and elution buffer containing biotinylated proteins was transferred to a new 1.5 ml LoBind protein tube (Eppendorf). 70 µl of sample was electrophoresed on a NuPAGETM 4-12% Bis-Tris protein gel (Invitrogen) ...
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bioRxiv - Genomics 2019Quote: ... Beads were then resuspended in 200 uL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and incubated at 65°C in a Thermomixer (Eppendorf) with interval mixing to dissociate antibodies from chromatin ...
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bioRxiv - Plant Biology 2019Quote: ... elution was performed with 30 μl of 1M Glycine (pH 2.0) buffer for 2 min in a Thermomixer (Eppendorf) at 1200 rpm ...
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bioRxiv - Genetics 2020Quote: ... buffer (10mM Tris-HCl pH 8.0, 0.5mM EDTA, 1M NaCl) in a 1.5ml Eppendorf Lobind tube (Eppendorf, 0030 108.051). DNA binding was performed in a thermo mixer (Eppendorf ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were eluted with 60 μL of buffer B (80% ACN, 0.1% formic acid in H20) and reduced in a Vacufuge plus (Eppendorf) to a final volume of 3 μL ...
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bioRxiv - Microbiology 2020Quote: ... the microbial pellet was resuspended in 500 μL lysis buffer (4% SDS (w/v) in 50 mM Tris-HCl buffer pH 8.0) followed by incubation in a Thermomixer Comfort (Eppendorf) at 95 °C for 10 min with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... we further purified the extracts by treatment with 1% sodium dodecyl sulfate in NaP-EDTA buffer and centrifugation at 13,000 rpm for 10 min (rotor FA-24×2, Eppendorf). This purification process was repeated two times ...
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bioRxiv - Molecular Biology 2022Quote: ... Elution buffer incubation time was extended to ~20 minutes and DNA was eluted into LoBind tubes (Eppendorf, Hamburg, Germany).
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bioRxiv - Genomics 2023Quote: ... Beads were then resuspended in 200 μL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and incubated at 65 °C in a Thermomixer (Eppendorf) with interval mixing to dissociate antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were washed with minimal washing buffer (50 mM Tris, pH 7.5; 150 mM NaCl) for 15 min and transferred to Protein LoBind tubes (Eppendorf). The washing procedure was repeated twice and beads were again transferred to new Protein LoBind tubes ...
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bioRxiv - Microbiology 2024Quote: ... coli cultures and mixed with resuspended in a 1X lysis buffer / Hot Acid Phenol solution in a 1.5 mL microcentrifuge tube on a thermomixer (Eppendorf) set to 65°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The bones of the hind limbs were cut transversally in the middle of the diaphysis and the bone marrow was harvested by centrifuging for 30 sec on 5500 g (after placing the bones with the opened sides down in PCR tubes placed in 1.5 ml Eppendorf tubes). The bone marrow was resuspended in culture medium ...
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bioRxiv - Immunology 2021Quote: ... Resulting cDNA was diluted 1:8 and subjected to endpoint RT-PCR using Taq DNA Polymerase (GeneDirex Inc., Taoyuan, Taiwan) in a Mastercycler Nexus (Eppendorf) or Bio-Rad T100 thermocycler ...
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bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
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bioRxiv - Neuroscience 2020Quote: ... 180 μL aliquots of solubilized GlyT1 cell membrane were dispensed in a 4°C-cooled 96-well PCR plate (Eppendorf) in triplicates ...
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bioRxiv - Microbiology 2020Quote: ... 190 μl of the lysate was combined with 10 μl of 20 mg/ml proteinase K in a PCR plate and incubated in a MasterCycler ProS (Eppendorf) for 60 min at 60°C for digestion ...
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bioRxiv - Developmental Biology 2020Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using FemtoJet or FemtoJet 4i (Eppendorf; 5252000013) micro-injectors ...
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bioRxiv - Neuroscience 2022Quote: ... Cells from each brain region were sorted in a separate A1 well of a 96 well PCR plate (Eppendorf, 951020401) containing 100 µl of PBS buffer with 0.3% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... 1mM PMSF(sigma), orange G dye (adopted form (von der Haar, 2007)) and moved to 384 wells PCR plate (Eppendorf) with reusable lid (4tetude) ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 96-well plate was sealed with a sterile and optically transparent sealing film (PCR-SP-S, AxySeal Scientific) and covered with a polystyrene plate lid (Eppendorf) that had silicone grease lining the edges ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the tip of the pipette was broken into a single nuclease-free 0.2 mL PCR-tube (Eppendorf AG, Hamburg, Germany), pre-filled with 9 µL Single Cell Lysis Solution and 1 µL Single Cell DNAse ...
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bioRxiv - Microbiology 2021Quote: ... was performed with 0.5 µg of ligated DNA with oligonucleotides 6212 and 6420 in a standard PCR program with a 3 min extension time on a Mastercycler thermocycler (Eppendorf). Phusion DNA polymerase (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Biochemistry 2021Quote: ... CD34-) (Supplementary Fig. 1) were sorted at single-cell resolution into a 384-well Eppendorf LoBind PCR plate (Eppendorf AG) containing 1 μl of lysis buffer (50 mM Triethylammonium bicarbonate (TEAB ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed with the realplex2 Mastercycler epgradient S automated real-time PCR system (Eppendorf®, New York City, NY) with standard reaction conditions (Table S8) ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time PCR was carried out using Maxima SYBR Green qPCR Master Mix (2X) (Molecular Biology) and Eppendorf Realplex System (Eppendorf) following the manufacturers’ protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transferred to a PCR-strip tube and heated for 3 min using a Mastercycler Nexus X2 thermal cycler (Eppendorf). After heating ...
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bioRxiv - Cell Biology 2023Quote: ... followed by ϕ29 polymerase heat inactivation at 65°C for 20 min and cooling at 4°C using PCR thermocycler (Eppendorf). Amplified C-circle DNA was diluted with 2X SSC buffer and loaded onto a dot-blot apparatus bedded with Hybond N+ membrane (RPN2020B ...