Labshake search
Citations for Eppendorf :
201 - 250 of 429 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and sonicated in chilled PBS (1 s on ...
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bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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bioRxiv - Biophysics 2022Quote: ... the DNA droplet-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). For the segregation of DNA droplets using enzymatic activity ...
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bioRxiv - Synthetic Biology 2022Quote: The concentration and quality of the plasmid solutions were determined by diluting 1 ul of plasmid solution in 4 ul of Tris EDTA buffer and loading 3 ul of the diluted solution on a μCuvette G1 (Eppendorf). Optical densities were measured at 260 nm and 280 nm using a BioSpectrophotometer and Fluorimeter (Eppendorf) ...
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bioRxiv - Biophysics 2022Quote: ... The aqueous solution was layered on top of the oil-surfactant mix in a volumetric ratio of 1:3 inside a microtube (Eppendorf). The tube was manually shaken for about 30 s until water-in-oil droplets formed ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 30 mycn mutant or WT zebrafish embryos at 3 dpf were transferred to 1.5mL Low binding microcentrifuge tubes (Eppendorf 022431021). Trypsin-EDTA solution (Beyotime ...
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bioRxiv - Microbiology 2021Quote: ... was performed with 0.5 µg of ligated DNA with oligonucleotides 6212 and 6420 in a standard PCR program with a 3 min extension time on a Mastercycler thermocycler (Eppendorf). Phusion DNA polymerase (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation reactions were carried out for the indicated proteins using rabbit reticulocyte lysate and SP cells at a final concentration of 3×106 cells/ml at 30°C for 40 min in a thermomixer (Eppendorf) set to 900 rpm ...
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bioRxiv - Biophysics 2020Quote: ... the DNA-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). Droplet formation was induced by manual shaking for about 4 s as described earlier.[26] For the oil-phase ...
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bioRxiv - Physiology 2020Quote: ... with 3 mM Tris(2-carboxyethyl)phosphine hydrochloride(TCEP-HCl) (Thermo Pierce) while shaking at 600 rpm in a thermomixer (Eppendorf). Samples were alkylated with 9 mM iodoacetamide (22 °C ...
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bioRxiv - Genomics 2024Quote: ... and washed in nuclease-free H2O before proceeding to MNase digestion with 1-2U of MNase for 3×106 cells that went on for 1-hour at 37C while shaking in a thermomixer (Eppendorf) at 550rcf ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transferred to a PCR-strip tube and heated for 3 min using a Mastercycler Nexus X2 thermal cycler (Eppendorf). After heating ...
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bioRxiv - Molecular Biology 2023Quote: ... the rehydrated gel pieces were overlaid with 30 μL of 0.01% ProteaseMAX in 50 mM ammonium bicarbonate and incubated at 37°C for 3 hours with shaking at 800 rpm (Thermomixer, Eppendorf). The digestion reaction was transferred to a fresh tube ...
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bioRxiv - Neuroscience 2023Quote: ... PFF oligomers were incubated with or without SVD-1a in PBS pH 7.4 for 3 d at 37 °C under quiescent condition in low retention Eppendorf tubes (Eppendorf, GE). Prior to injection ...
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bioRxiv - Microbiology 2023Quote: ... The beads were pelleted (600 x g, 3 min) and the supernatant transferred to a fresh low protein binding tube (Eppendorf). The beads were washed with 1 x 200 µL HPLC-grade water and the washes combined with the original supernatant ...
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bioRxiv - Microbiology 2020Quote: ... After an incubation period of 30 min under shaking (300 rpm, Thermomixer Comfort Typ 5, Eppendorf) at room temperature 65 µL chloroform and bidest ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated at 37°C in humidified 5% CO2 incubators (Eppendorf, GmBH or Thermo Scientific, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Cultures were grown in a humidified incubator with 5% CO2 and maintained at 37°C (Eppendorf). The medium was changed every four days ...
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bioRxiv - Cell Biology 2021Quote: ... and then the total elution fractions were dried for 5 h using a Speed-vac (Eppendorf). The samples were stored at –20°C before use in LC-MS/MS analyses.
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bioRxiv - Cancer Biology 2020Quote: ... 2 million cells were added to a 5 mL DNA LoBind tube (Eppendorf, cat. no. 0030108310), centrifuged at 400 x g for 4 min ...
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bioRxiv - Systems Biology 2022Quote: ... The plate was then centrifuged at 1,000 rpm for 5 min using a centrifuge (Eppendorf, 5810R) that was precooled at the desired temperature (e.g ...
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bioRxiv - Microbiology 2020Quote: ... The plates were then spun at 260g for 5 min at room temperature (Eppendorf 5810R centrifuge) and incubated at 33°C ...
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bioRxiv - Biochemistry 2021Quote: ... and 5% CO2 in a New Brunswick S41i CO2 incubator shaker (New Brunswick Eppendorf, Hamburg, Germany) for 4 days and passaged twice to 0.5×106 cells/mL in a 250 mL and 500 mL shake flask ...
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bioRxiv - Immunology 2020Quote: ... and incubated with stirring at 500 rpm for 5 min at 85°C (Eppendorf, ThermoMixer C). Lysates were either sonicated (Sonifier 250 ...
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bioRxiv - Microbiology 2022Quote: ... Bone marrow cells were pelleted by centrifuging at 1500 rpm for 5 min (Eppendorf centrifuge R5810). Red blood cells were lysed by resuspending the cell pellet in 1 mL of red blood cell lysis solution (150mM NH4Cl ...
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bioRxiv - Neuroscience 2023Quote: ... were mixed and heated for 5 min at 65°C in a Thermal Cycler (Eppendorf, Germany). Next ...
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bioRxiv - Evolutionary Biology 2022Quote: ... its volume was determined using an Eppendorf Xplorer 5–1000 μL electronic pipette (Eppendorf, Hamburg, Germany) and kept homogenous by agitation ...
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bioRxiv - Neuroscience 2023Quote: ... were mixed and heated for 5 min at 65°C in a Thermal Cycler (Eppendorf, Germany). After this step 4 μl of 5x first-strand buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were centrifuged at 1000 rpm for 5 minutes in a MiniSpin Plus microcentrifuge (Eppendorf, Germany). Washed cells were resuspended in 150 μL of FACS buffer and treated with anti-mouse MHC class I antibody conjugated to PE (Clone:28-14-8 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µL of each reaction was dried using a centrifuge concentrator (Vacufuge plus, Eppendorf, Hamburg, Germany) for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... pH 5) incubated at 37°C for 20h at 300 rpm in a ThermoMixer C (Eppendorf).
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bioRxiv - Systems Biology 2023Quote: ... The samples were then distributed evenly over 2 x 5 mL reaction tubes (Eppendorf, Hamburg, Germany) and spun at 14,200 g for 15 min ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... The 5 ml overnight culture was centrifuged at 8,000 rpm (Eppendorf MiniSpin F-45-12-11) for three minutes ...
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Malaria parasite HOPS/CORVET complexes are critical for endocytosis and invasion organelles functionbioRxiv - Cell Biology 2024Quote: ... 200 mM hypoxanthine and 2-5% fresh human RBCs (B+; provided by Universität Klinikum Eppendorf, Hamburg). Cultures were maintained at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The tubes were then sonicated for 5 min and incubated on a thermomixer (Eppendorf, Hamburg, Germany) at 600 rpm for 60 min at 22°C ...
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bioRxiv - Biophysics 2024Quote: ... The requisite number of cells (2− 5 × 105) was pelleted by centrifugation (#5810, Eppendorf, Hamburg, Germany) at 200g for 3 minutes and the supernatant was discarded ...
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bioRxiv - Cell Biology 2024Quote: ... then mixed at 950 rpm for 5 min at 10 °C in an Eppendorf Thermomixer (Eppendorf South Pacific Pty Ltd. ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µM AQT0615) and 10 µL were added in each well with a multichannel pipette (Eppendorf). The 0 % control wells were filled with 10 µL of pure reaction buffer ...
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bioRxiv - Bioengineering 2020Quote: The concentration of nitrogen in the media was monitored by taking 1 ml samples from the cultures at various time points and centrifuging them for 3 min at 16900 × g (benchtop 5430R centrifuge; Eppendorf, Germany). The supernatant was retained and the nitrogen concentration was determined using the method of Scheiner71 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were incubated with the dye and shaken for 20 minutes at room temperature in the dark, then washed by centrifugation (3 min, 8000 RPM, 28 °C (Eppendorf 5430R)) and resuspension in M2G three times ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were vigorously vortex-mixed for 3 min and centrifuged at 14,000 rpm for 10 min at 4 °C (Eppendorf 5804 R). The clear supernatant was then transferred to a separate vial ...
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bioRxiv - Plant Biology 2022Quote: ... annealing at 51 °C for 30 s and extension 72 °C for 30 s for 25 cycles by final extension 72 °C for 3 min and reaction hold at 4 °C in a thermocycler (Gradient thermocycler® Eppendorf). Amplified gene products were sequenced using the Sanger sequencing method ...
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bioRxiv - Genetics 2024Quote: ... the samples were washed 3 times with 500 μl Perm wash (centrifugation for 2 minutes at 1600 rpm (Eppendorf centrifuge 5415R)) ...
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bioRxiv - Microbiology 2024Quote: Sample were retrotranscribed in cDNA using 1 µL of RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted material was aliquoted into 3 samples in low binding polypropypene tubes (nominally 1.5 mL) and dried in a vacuum concentrator (Vacufuge® plus; Eppendorf™) at room temperature without additional heating for about 3h ...
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bioRxiv - Microbiology 2023Quote: ... All microinjection procedures were carried out on 3 day post-fertilization embryos with the help of FemtoJet Microinjector and PatchMan NP2 Micromanipulator (Eppendorf, Germany). Embryos were divided into three groups-mock ...
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bioRxiv - Neuroscience 2024Quote: ... 300 μL of flow-through from the initial amicon concentration step above (< 3 kDa MWCO) was derivatised (alkylated) with NEM 20 mM and incubated in a thermomixer (Eppendorf, UK) at 25oC for 45 min at 850 rpm in the dark ...
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bioRxiv - Microbiology 2024Quote: Samples were retrotranscribed in cDNA using 1 µL of extracted RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
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bioRxiv - Physiology 2024Quote: ... 3 mL of 60% ACN / 0.1% FA (v/v) was loaded and collected into low-binding polypropylene tubes (Eppendorf, Hamburg, Germany). Samples were dried out using a SpeedVac (Eppendorf ...
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bioRxiv - Neuroscience 2024Quote: ... They were then resuspended to a concentration of ∼800-3000 nuclei per uL across 3-4 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512) in NSB ...