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Citations for Eppendorf :
201 - 250 of 735 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 0.6 mM DTT) and pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Pulverized frozen tissue and pelleted nuclei from gentleMACS M-tubes were each split into two further aliquots ...
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bioRxiv - Neuroscience 2020Quote: ... Isolated brain nuclei were pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA ...
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bioRxiv - Systems Biology 2021Quote: ... 5 ml of working volume was used in the batch culture at 37°C on a rotary shaker (Eppendorf). The pH of the growth medium was set to 7 ± 0.05.
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bioRxiv - Genomics 2022Quote: ... Multiple 20µm tissue curls were cut per tissue and collected in pre-cooled 5 ml tubes (Eppendorf, Hamburg, Germany) ensuring no thawing while transferring and stored on dry ice until processing ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). All used cell lines were confirmed to be mycoplasma free by using MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Genetics 2021Quote: ... A study pool quality control (SPQC) sample was prepared by combining 5 µL of supernatant from each sample into a 1.5 mL tube (Eppendorf). Stable Isotope Labeled (SIL ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were put on ice for 30 min and precipitates were spun down for 5 min at 20,000 g at 4°C in a tabletop centrifuge (Eppendorf). After one wash with ice-cold acetone ...
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bioRxiv - Cell Biology 2021Quote: ... spun at 1,000 x g at −9°C for 5 minutes then aliquoted into lo-bind 2ml centrifuge tubes (Eppendorf) and snap frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... OD660 of 0.3-0.5 mid-log) were harvested by centrifugation (5,000 x g, 5 min at 4°C) (Eppendorf centrifuge 5430R). The supernatant was immediately discarded ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 4°C (3220 g for 5 min, A-4-81 rotor, Eppendorf, Enfield, CT) to remove intact cells and debris ...
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bioRxiv - Cell Biology 2022Quote: ... protease and phosphatase inhibitors and PMSF (10 minutes on ice) and centrifuged at 13,300 rpm for 5 min at 4OC (Centrifuge 5810 R; rotor A-4-81; Eppendorf). The supernatant (whole cell lysates ...
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bioRxiv - Microbiology 2022Quote: Sheep or horse erythrocytes were washed three times with sterile PBS (pH=7.4) at 300⍰×⍰g for 5⍰min in a refrigerated centrifuge (Eppendorf). These washed erythrocytes suspension was lysed with 0.1% saponin and the concentration of oxy-hemoglobin in this preparation was determined using the QuantiChrom™ Hemoglobin Assay Kit (BioAssay Systems) ...
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bioRxiv - Microbiology 2022Quote: ... supernatants containing planktonic bacteria were removed from cultures and bacteria were pelleted down by centrifugation at 13,000 rpm x 5 min in a refrigerated micro-centrifuge (Eppendorf). In another set of experiments ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae strain to 5 mL of YPD media and incubating in an orbital shaker incubator (Innova 42, Eppendorf, UK) at 30 °C and 250 rpm overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and kept at 37°C with 5% CO2 in an incubator (New Brunswick Galaxy 170 S, Eppendorf, Schönenbuch, Switzerland).
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bioRxiv - Biophysics 2024Quote: ... to a final concentration of 200 nM PCt and 0.1-8 M GdmCl in 5 ml Protein LoBind tubes (Eppendorf). To avoid time-dependent photobleaching ...
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bioRxiv - Genetics 2024Quote: ... the samples were washed 3 times with 500 μl Perm wash (centrifugation for 2 minutes at 1600 rpm (Eppendorf centrifuge 5415R)) ...
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bioRxiv - Cell Biology 2020Quote: Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 5 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Cell Biology 2020Quote: ... we centrifuged the crude lysate at 500 g for 5 min at 4°C in a microfuge (Eppendorf, model 5424R) to remove cell debris ...
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bioRxiv - Immunology 2021Quote: ... Equal volume of Mycobacteria tuberculosis H37RA-CFA was mixed with MOG/PBS in a 5 mL protein Lobind tube (Eppendorf), followed by emulsification in ice water through sonication ...
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bioRxiv - Cell Biology 2022Quote: ... Absorbance was measured at 600 nm after every 12 h intervals till 4 to 5 days using a UV-visible spectrophotometer (Eppendorf).
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bioRxiv - Biochemistry 2020Quote: ... The resin was pelleted down by centrifugation at 4°C (1258 g for 5 min, Eppendorf #A-4-81 rotor) and washed with ice-cold 50 mL Binding buffer composed of 10 mM imidazole (pH 7.4) ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μL of 25 mM aqueous NH4HCO3 were added to each tube and incubated for 5 min at room temperature on a ThermoMixer (Eppendorf) at 700 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... All solution was removed and 100 μL of acetonitrile was added to each tube and incubated for 5 min at room temperature on a ThermoMixer (Eppendorf) at 700 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mg ml−1 lactic acid in water) and added to 5 mg of pre-washed TiO2 beads and incubated for 30 min on a Thermomixer (Eppendorf) at 1200 rpm to keep the beads in suspension ...
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bioRxiv - Bioengineering 2021Quote: ... a 96-well plate pre-filled with 20 μL pure water and sorted with designated cells per well was frozen on dry ice for 5 min and heated by ThermoMixer C (Eppendorf) at 95 °C for 10 min followed by spinning down at 3000 rpm for 1 min ...
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bioRxiv - Cell Biology 2021Quote: ... was harvested by centrifugation at 300 × g for 5 min in a swing out 5804 S-4-72 rotor (Eppendorf). Baculovirus infected insect cell (BIIC ...
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bioRxiv - Synthetic Biology 2021Quote: ... The fractions with highest concentrations (200–400 μg mL−1) were stored as 5–10 μL aliquots in Protein LoBind tubes (Eppendorf) at –80 °C.
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37°C and 5% CO2 in a humidified incubator (Galaxy 170S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
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bioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37 °C and 5% CO2 in a Galaxy 170S CO2 humidified incubator (Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Homogenates were centrifuged at 1000 rpm for 5 min at 4°C in a table centrifuge (5424 R, Eppendorf, Germany). The supernatant was transferred to a new tube and again centrifuged at 4°C for 5 min at 1400 rpm ...
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bioRxiv - Immunology 2021Quote: RBD219-WT and RBD219-N1C1 were expressed at the 5 L scale using a Celligen 310 benchtop fermentation system (Eppendorf). For the RBD-WT ...
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bioRxiv - Systems Biology 2023Quote: ... Homogenized islets were filtered with 30 µm filter (CellTrics, Sysmex) and pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Nuclei were resuspended in 400 µL of sort buffer (1% fatty acid-free BSA ...
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bioRxiv - Systems Biology 2023Quote: ... were added without disturbing the pellet and nuclei were pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Supernatant was gently removed without disturbing the pellet and leaving ∼2-3 µl behind ...
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bioRxiv - Systems Biology 2023Quote: ... and incubated on ice for 1 min before pelleting with a swinging-bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Supernatant was gently removed and ∼50 µl were left behind to increase nuclei recovery ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting injection micropipette was backfilled with 5-10 μL of 500 μg/mL fluorescein solution and was mounted on the microinjection manipulator (InjectMan 4, Eppendorf) connected to a pneumatic microinjection pump (FemtoJet 4i ...
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bioRxiv - Bioengineering 2023Quote: ... in LB (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) grown in a 10 L BioFlo 320 Fermenter (Eppendorf). At inoculation ...
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bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... Eluted DNA was then passed through the column a second time and concentrated for hybridization to 5 μl with a SpeedVac Concentrator (Eppendorf).
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bioRxiv - Cell Biology 2024Quote: ... The culture was maintained in a humidified atmosphere containing 5% CO2 at 37 L in a cell incubator (Eppendorf, Germany). The medium was changed daily ...
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bioRxiv - Neuroscience 2023Quote: ... After centrifuging the samples for 15 minutes at 5°C at 4000 rpm on bench top centrifuge 5810R (Eppendorf, USA), 600 μL of dichloromethane (bottom layer ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cell suspension was then transferred to a pre-chilled electroporation cuvette and after approximately 5 – 10 min electroporated in the eporator electroporator (Eppendorf) set to 1850 V ...
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bioRxiv - Cell Biology 2023Quote: ... After a 5-minute incubation the protein concentration was determined through measurement of the OD595 with a photometer (BioPhotometer plus, Eppendorf). Finally ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 x 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Neuroscience 2023Quote: Neurons remained in medium and were incubated in ∼1% O2 (5% CO2, 37°C) for 360 min in a triple-gas incubator (Eppendorf) and then returned to normoxic conditions (21% O2 ...
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bioRxiv - Cell Biology 2023Quote: ... is harvested by centrifugation at 300 × g for 5 min in a swing out 5804 S-4-72 rotor (Eppendorf). P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: All cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). They have also been regularly tested for mycoplasma contamination by examining the samples for extracellular DNA staining with SiR-DNA (100 nM ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 and 0.1% Tween 20 in water) and centrifuged for 5 min at 500 g in a swinging bucket rotor centrifuge (5920R, Eppendorf). Nuclei pellets were resuspended in 1X Tagmentation Buffer (10 mM Tris pH 7.4 ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting pellet was re-suspended in 5 mL of modified CIB medium and centrifuged at 16,000 gav for 10 min in 5415R refrigerated centrifuge (Eppendorf, USA). The resulting supernatant was the Sucrose Gradient Load (SGL) ...
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bioRxiv - Neuroscience 2022Quote: ... and Sample Reducing Agent (10x) and incubated for 5 min at 70°C and 1100 rpm using a ThermoMixer comfort (Eppendorf).