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Citations for Eppendorf :
151 - 200 of 210 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 30 mycn mutant or WT zebrafish embryos at 3 dpf were transferred to 1.5mL Low binding microcentrifuge tubes (Eppendorf 022431021). Trypsin-EDTA solution (Beyotime ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in 52 μL of 0.1X TE for 30 min while briefly vortexing and centrifuging (Eppendorf 5424R) every 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation reactions were carried out for the indicated proteins using rabbit reticulocyte lysate and SP cells at a final concentration of 3×106 cells/ml at 30°C for 40 min in a thermomixer (Eppendorf) set to 900 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... The reaction mix was incubated for 30 min at 25°C with 700 rpm shaking in a ThemoMixer C (Eppendorf) and subsequently quenched for 20 min by adding Tris-HCl (final concentration 50 mM) ...
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bioRxiv - Molecular Biology 2022Quote: ... The kinase reaction was carried out at 30 °C for thirty minutes with shaking (300 rpm) in a Thermomixer (Eppendorf). The reaction was stopped by addition of 5 μL of 4X SDS-PAGE loading buffer [250 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... The kinase reaction was carried out at 30 °C for two hours with shaking (300 rpm) in a Thermomixer (Eppendorf). The reaction was stopped by addition of 5 μL of 4X SDS-PAGE loading buffer [250 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The assay plates were covered with a self-adhesive aluminium foil and incubated at 30°C for the indicated time point(s) in an Eppendorf ThermoMixer C (Eppendorf) equipped with a ThermoTop 288 and a SmartBlock™ PCR 384 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reaction was performed in a final volume of 60 μL for 60 min at 30°C under stirring (750 RPM, Thermomixer, Eppendorf). γS-ATP was removed by dialyzing the reaction mix against kinase buffer ...
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bioRxiv - Microbiology 2023Quote: ... The sample was vortexed for 30 seconds before being centrifuged for 15 mins at 4°C at 1739 x g (3000 rpm, Eppendorf 5810R benchtop centrifuge ...
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bioRxiv - Genetics 2023Quote: ... This mix was incubated for 30 minutes at 37°C to form ribonucleoprotein complexes and injected into hermaphrodite adult gonads using a Transjector 5246 (Eppendorf). F1 progeny were singled out from plates displaying a high proportion of the dumpy (dpy-10 ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 min-4 h at 76°C in a heat block (speed of 300 rpm; ThermoMixer F1.5, Eppendorf, Germany) (Wagner et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated at 37°C at 600 rpm with interval mixing (30 seconds mix, 2 minutes pause) on a Thermomixer C (2231000667, Eppendorf). After the last digestion ...
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bioRxiv - Developmental Biology 2024Quote: ... organoids were treated with 200 µL of probe hybridization buffer for 30 min at 37 ºC inside a Thermomixer covered by the ThermoTop (Eppendorf) to avoid evaporation of the buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Conditioned media and cells were then collected as follows: concentrated PI cocktail dissolved in 30 µL PBS was first filled in the collection tube (low-binding; Eppendorf) and conditioned media was carefully aspirated from the cell layer ...
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bioRxiv - Cancer Biology 2023Quote: 20-30 mg sections of snap frozen zebrafish liver tissue were transferred to pre-chilled Safe-Lock tubes (Eppendorf, 022363352) containing a cold 5/16 in ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred to a new tube and solvents were removed by evaporation at 30°C for 90 min at reduced pressure (Concentrator Plus, Eppendorf). The samples were then fully dried in a freeze dryer and stored at −80°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... as glucose acceptor and 0.1 mM UDP-Glucose as glucose donor in a final reaction volume of 1 mL for 20 min at 30 °C and 800 rpm using a Thermoshaker (Eppendorf). Crude extracts used to test individual assemblies were diluted to an exact protein concentration of 50 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: Ear tags were obtained from mice at the age of 3 weeks and DNA was isolated by boiling the eartags for 30 min in 200 μl 50 mM NaOH at 95°C (ThermoMixer C, Eppendorf). Afterwards ...
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bioRxiv - Cell Biology 2023Quote: ... Transposase reactions were carried out by incubating samples at 37°C for 30 min under mild agitation (300 rpm on a Thermo-mixer C, Eppendorf). Once the incubation was completed,sample tubes were placed on ice and the transposed DNA fragments from each sample were purified using a Qiagen MinElute PCR Purification Kit following manufacture’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... was added and incubated for 30 min at room temperature in the dark while shaking at 1500 rpm (Thermomixer C, Eppendorf). The IAM was quenched with 11 mM DTT ...
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bioRxiv - Microbiology 2024Quote: ... which were centrifuged at 3220 x g for 30 min at room temperature (Eppendorf A-4-62 Swing Bucket Rotor). The interface layer containing microbial cells was recovered ...
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bioRxiv - Cell Biology 2024Quote: ... the cell lysate was collected and agitated for 30 min at 800 rpm at 4 °C in a Thermomixer (Eppendorf). Afterwards ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of the reaction mixture were added to 30 μL BCA reagent solution in a 384-well PCR plate (Eppendorf twin.tec PCR MTP 384 ...
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bioRxiv - Microbiology 2021Quote: ... resuspended at a final concentration of 1x106 cells/ml in minimal media in a 1.5 ml tube and grown at 30°C with 800 rpm in an Eppendorf Thermomixer (Eppendorf, Hamburg, Germany). Cell size was analyzed after 4 days ...
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bioRxiv - Microbiology 2021Quote: ... Serum was separated from blood by centrifugation at 8,000 RPM for 30 minutes at 4 °C in an Eppendorf 5424R centrifuge (Eppendorf, Enfield, CT). Serum was heat inactivated by incubating at 56 °C for 30 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated at 30 °C for 20 hours (lid temperature 37 °C) in an Mastercycler pro384 vapo.protect thermocycler (Eppendorf, Hamberg, Germany). Scaled-up (15 μL ...
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bioRxiv - Synthetic Biology 2020Quote: ... Solutions were then spun down at 13,000 RPM for 30 minutes at 4°C in a benchtop micro-centrifuge (Eppendorf Centrifuge 5424) to remove cells and other solid debris ...
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bioRxiv - Zoology 2021Quote: ... Thereafter a first centrifugation step was performed to discard excessive non-degradable matter at 10,000 x g for 30 sec (standard rotor F45–24–11, Eppendorf centrifuge 5415R). The supernatant was collected and added to 900 µl of fresh PBS and vortexed ...
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bioRxiv - Microbiology 2021Quote: ... the pre‐cultures were incubated at 30°C with a shaking speed of 250 rpm (Innova 44, Eppendorf New Brunswick, DE).
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bioRxiv - Bioengineering 2023Quote: ... The resulting culture microplate was incubated at 30 °C for 72 hours at 350 rpm in a Thermomixer C (Eppendorf, Germany) and covered with an adhesive gas permeable membrane (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... bacterial stocks were grown at 30°C for 2 days to an OD600 of 0.4 approximately 6.3×106CFU/mL (Eppendorf, BioPhotometer; Hamburg, Germany). Bacteria were washed with sterile PBS and aspirated through a 27G needle against the side of the tube multiple times to break up bacterial clumps ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was used to fractionate the cell lysate (at 4 °C for 30 min at 4,416 g, Eppendorf, Centrifuge 5804 R) and at 4 °C for 1 hour for ultracentrifugation (70,658 g ...
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bioRxiv - Genomics 2023Quote: ... Individual DNA were normalized to a common concentration of 30 ng/µl and combined to a single tube using the liquid handling robot epmotion 5407 (Eppendorf, Germany). Sequencing library preparation and high-throughput sequencing were performed by The Centre for Applied Genomics of the Hospital for Sick Children ...
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bioRxiv - Biochemistry 2024Quote: ... and the sample was incubated at 60 °C and 1,000 rpm for 30 minutes on an Eppendorf ThermoMixer Temperature Control Device (Eppendorf, Hamburg, Germany). Iodoacetamide (IAA ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then exposed to phages at indicated MOIs or incubated untreated for 30 min in a 2 mL Eppendorf tube under shaking conditions (<650 rpm in an Eppendorf ThermoMixer). For TIRF microscopy ...
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bioRxiv - Biochemistry 2024Quote: ... The plate was then centrifuged at 1000 rpm for 30 s (5804/ 5804 R – Benchtop Centrifuge, Eppendorf North America, CT, USA), mixed on a plate shaker (Siemens DPC MicroMix 5 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested by centrifugation at 5478 g (7000 rpm with Eppendorf F-35-6-30 Rotor for 5430/5430R rotor) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 30 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Immunology 2021Quote: ... The 80 µL aliquot was lyophilised for 4 h at 30 °C with the aid of a vacuum concentrator (Eppendorf Concentrator 5301) attached to a refrigerated condensation trap (Savant) ...
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bioRxiv - Biophysics 2020Quote: ... The cell suspension was subsequently centrifuged for 30 min at 4°C and 3250 × g (Eppendorf Swing-bucket rotor A-4-62). The supernatant was discarded ...
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bioRxiv - Cell Biology 2021Quote: ... cells were recovered in 1 mL YPD (1% yeast extract, 2% Bacto peptone, 2% D-glucose) at 30°C in a ThermoMixer C (Eppendorf, Hamburg, Germany) for 3 hours (200 RPM ...
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bioRxiv - Genetics 2022Quote: ... we inoculated yeast strains into 400 µL of liquid SC -lys medium with G418 for overnight growth in 2 mL 96 well plates at 30 °C with 1000 rpm mixing on a MixMate (Eppendorf, Hamburg, Germany). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... sonicated for 15 min and vigorously mixed at 5°C for 30 min with 2000 rpm on a ThermoMixer (Eppendorf, Hamburg, Germany). Samples were centrifuged for 5 min at 18000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... and the upper phase (water-soluble metabolites) was evaporated for 30 minutes at 35°C under nitrogen and dried in SpeedVac (Eppendorf, Hamburg, Germany) at 15 °C overnight ...
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bioRxiv - Immunology 2023Quote: ... The beads were resuspended with the lysed cells for 30 minutes at 25 °C with orbital mixing at 1500 rpm using an Eppendorf Thermomixer (Eppendorf, Hamburg, Germany). Samples were centrifuged and placed on a magnet ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
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bioRxiv - Biochemistry 2022Quote: ... samples were prepared in 96 well microtiter plates by adding 12.5 μL reaction supernatant to 50 μL acetonitrile with 1% v/v trifluoroacetic acid (TFA) followed by centrifugation (2,204 g, 30 min; A-2-DWP rotor, Eppendorf AG, Hamburg, Germany) and transferring of 50 μL centrifugation supernatant into 150 μL MilliQ H2O ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction was started either by the addition of enzyme or substrate followed by incubation at 30 ºC using a thermomixer with horizontal agitation (500 rpm; Eppendorf, Hamburg, Germany) for 60 minutes (unless stated otherwise) ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was centrifuged for 30 min at 15000 rpm at 4°C in the centrifuge Eppendorf 5702RH (Eppendorf SE, Hamburg, Germany). The pellet was resuspended in the 1/5 of the in initial volumes in PBS buffer (ThermoFischer Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 μL RNase A (10mg/mL) was added to each sample and incubated for 30 minutes at 37°C on an Eppendorf ThermoMixer C (Eppendorf AG, Hamburg, Germany). Subsequently ...