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Citations for Eppendorf :
1251 - 1300 of 1303 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... the DNA was eluted from the beads by incubating with 150μl elution buffer (100mM NaHCO3 and 1% SDS) for 20 mins at 65°C with vortexing using Eppendorf ThermoMixer C (Eppendorf). The supernatant was collected ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). They were then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Developmental Biology 2024Quote: ... Another 1 ml of Washing buffer B was added and transferred to 1.5 ml Protein lo-bind tubes (Eppendorf, 0030108116) with beads ...
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bioRxiv - Biochemistry 2024Quote: Hydrolysis reactions were performed in the presence of 1% starch and 15.6 nM of free purified protein WT or H222Q in a final volume of 1 mL at 85 °C and 500 rpm in a thermoblock mixer (Eppendorf). Soluble starch (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA (1:30 dilution) was utilized for 30-cycle three-step PCR in Mastercycler Ep Gradient Auto thermal cycler (Eppendorf) using Q5® High-Fidelity 2× Master Mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in 1ml 1 M sodium chloride and boiled at 100 °C for 20 min in a heating block (ThermoStat plus, Eppendorf). The suspension was centrifuged at 10,000 x g for 5 min ...
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bioRxiv - Biophysics 2024Quote: ... The monomer samples were then each concentrated to 1 mM and incubated at 37 °C and 200 rpm (orbital shaker, Eppendorf) for 3 weeks ...
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bioRxiv - Cell Biology 2024Quote: ... a mix of pre-polymerized mother filaments (formed from 1 μM 10 % AlexaFluor-568-labeled G-actin incubated in F-buffer in an Eppendorf tube for 2 hours ...
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bioRxiv - Biophysics 2021Quote: Bacterial suspensions were incubated with different concentrations of AMPs in buffer for 1 hour at 37 °C (incubator Thermomixer C, Eppendorf, Germany). Reference samples (no AMPs ...
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The effect of hybridization on transposable element accumulation in an undomesticated fungal speciesbioRxiv - Genomics 2020Quote: ... 40 μl of cultures at 0.2 OD595 ml-1 were added to the plates using an Eppendorf epMotion 5075 liquid handling platform (Eppendorf, Hamburg, Germany). Replicates were positioned according to the predefined randomized scheme in side-by-side pairs ...
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bioRxiv - Plant Biology 2020Quote: ... Bacterial pellets obtained from 1 mL of cell culture centrifuged at 13,000 rpm (Eppendorf 5424 Centrifuge with Fa-45-11 Rotor) were thoroughly washed three times with 1 mL fresh LB medium to remove residual antibiotics and were re-suspended in 100 µL liquid LB medium ...
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bioRxiv - Biophysics 2021Quote: ... Concentration of the diluted solutions were confirmed by absorbance at 412 nm using an extinction coefficient ε412 of 36,000 M−1cm−1 (Eppendorf BioSpectrometer Kinetic). All dye solutions were encased in aluminum foil and stored at 4 °C for up to 7 d ...
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bioRxiv - Neuroscience 2022Quote: ... were diluted in BME (1:10) and locally injected into the outer SVZ by using a FemtoJet® 4i microinjector (Eppendorf). After 72 h ...
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bioRxiv - Microbiology 2022Quote: A lysate sample (1 ml) with 108 PFU/ml was centrifuged at 20,000 g (centrifuge 5430R, rotor FA-45-24-11HS; Eppendorf, Hamburg Germany) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the injection needle was loaded with the injection mixture calibrated using a microscale graticule to deliver 1-1.7nl per injection and each egg was injected (FemtoJet 4x, Eppendorf, Hamburg, Germany), once ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of MTBE was added and the solution was incubated for 1 hour at room temperature with continuous shaking at 600 rpm (Eppendorf, MixMate). Next ...
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bioRxiv - Synthetic Biology 2023Quote: ... then 1 mL aliquots were placed in triplicate in plastic-bottomed black 24-well plates (Eppendorf Cell Imaging Plates ref. 0030741005), and the plates were placed onto a custom made light plate apparatus (LPA)27 to control blue light illumination in each well (λ = 460 nm ...
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bioRxiv - Microbiology 2023Quote: ... by adding 50 μL of a 2 mg/mL stock solution and 25 μL of 50 mM CaCl2 and incubating for 1 h at 37 °C with agitation at 300 rpm in a Thermomixer comfort (Eppendorf, Germany). The cell wall material was pelleted and washed two times with 2 mL of ultrapure water (21000 g ...
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bioRxiv - Neuroscience 2023Quote: ... was added to lyophilized Aβ1-42 peptide to a final concentration of 1 mM and centrifuged in a speed vac (Eppendorf, Hamburg, Germany). The peptide pellet was re suspended in DMSO at a 5 mM concentration and sonicated in a 37⁰C water bath for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... was adjusted to 50 ng/μL with 50% ACN containing 0.1% TFA and dispensed in 10 μL each into normal tubes (1.5-mL safe-lock tube, CAT# 0030120086, Eppendorf, Hamburg; Germany), normal polypropylene (PP ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... A 1 ml sample of overnight suspension was centrifuged for five minutes at 8,000 rpm (Eppendorf MiniSpin F-45-12-11), the pellet resuspended in 1 ml PBS ...
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bioRxiv - Biochemistry 2023Quote: ... The cellulase-LPMO synergy was assessed by performing reactions with crystalline Avicel (1 % w/v) in 50 mM sodium phosphate buffer (pH 6.0) using a thermomixer (Eppendorf, Hamburg, Germany) incubated at 30 °C with horizontal agitation (1000 rpm) ...
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bioRxiv - Physiology 2024Quote: Protein samples were dissolved in 8 M urea in 50 mM ammonium bicarbonate (pH 7.8) by vortexing for 1 hour (Eppendorf ThermoMixer F1.5). 5 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of formic acid 1% were placed in each Millipore Microcon 30 MRCFOR030 Ultracel PL-30 before centrifugation at 14,500 rpm (Eppendorf 5424 centrifuge) for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... in PBS in a 15mL tube and spun at 1000g for 10 min at room temperature with acceleration and brake set to 1 (Eppendorf 5810R). The supernatant was discarded ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of 108 PFU was centrifuged at 20,000 g (centrifuge 5430 R, rotor FA- 45-24-11HS; Eppendorf, Hamburg, Germany) for 2h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of 108 PFU was centrifuged at 20,000 g (centrifuge 5430 R, rotor FA-45-24-11HS; Eppendorf, Hamburg, Germany) for 2h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were eluted with 60 μl of 80% acetonitrile/1% ammonia and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were dissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were eluted with 60 μl of elution buffer (80% acetonitrile/1% ammonia) and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were redissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Biophysics 2022Quote: ... The cultures were then induced with IPTG by adding 10 uL of 100 mM (final concentration approximately 1 mM) per well with an electric repeater pipette (Eppendorf, E4x series), and grown for another 4 h at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/ml of hygromycin and correct genome editing was confirmed by Sanger sequencing (Macrogen Europe ...
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bioRxiv - Developmental Biology 2020Quote: ... Microinjection of young adult hermaphrodite germlines was done using an inverted microinjection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). In cases where the sgRNA target site was not disrupted by sequence integration ...
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bioRxiv - Microbiology 2022Quote: ... 4.6×106 LMBs or NMBs were added and samples incubated at 37 °C for 1 hour on a thermomixer (60 rpm; Eppendorf, Hamburg, Germany). After this step ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Systems Biology 2024Quote: ... are then added to the sample and incubated at 56°C for 1 hour at 1000 RPM (ThermoMixer C, Eppendorf, Hamburg, Germany). 37µl of 500mM chloroacetamide (50mM final concentration ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng corresponding pEcgRNA-N20 and 400 ng donor DNA were co-electrotransformed into 100 uL competent cells in 1 mm gap electroporation cuvette by applying 2.5 kV pulse in electroporator (Eppendorf™ Eporator™). After adding 900 uL of cold SOC medium ...
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bioRxiv - Biochemistry 2024Quote: ... A second gDNA purification was coupled with reverse transcription of RNA (1 μg) into cDNA with the SuperScript IV VILO ezDNase system (Thermo Fischer Scientific) using an Eppendorf Mastercycler (Eppendorf, Hamburg, Germany). cDNA samples were entered into a custom TaqMan low density array (Thermo Fischer Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Lyophilized peptide was dissolved in 100% HFIP solvent to a final concentration of 1 mM and centrifuged in a speed-vac (Eppendorf, Hamburg, Germany). Peptide pellet was then resuspended in DMSO to a final concentration of 5 mM followed by sonication at 37°C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... castellanii trophozoites were seeded at the log growth phase (1 × 104 cells per well) in a 96-well white microplate (Eppendorf, Hamburg, Germany). Incubation with medium containing varied nitroxoline concentrations ranging from 0 to 100 μM was performed ...
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bioRxiv - Biochemistry 2021Quote: ... Production of CjLPMO10AFL was achieved by fed-batch fermentation of the expression strain in a 1-liter fermenter (DASGIP® benchtop bioreactors for cell culture; Eppendorf, Hamburg, Germany), essentially as described previously (44) ...
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bioRxiv - Microbiology 2020Quote: ... printed arrays were moved to individual 1 mL centrifuge tubes containing 200 μL of M9 medium and melted at 55°C for 1 min in a thermomixer (Eppendorf ThermoMixer® C) shaking at 1000 rpm ...
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bioRxiv - Microbiology 2022Quote: ... Two-microliter aliquots of resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
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bioRxiv - Microbiology 2022Quote: ... Two μl aliquots of the resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
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bioRxiv - Bioengineering 2024Quote: ... 40 µL of clarified lysate was aliquoted into full-skirted PCR plates and incubated at the reported temperature for 1 h using a gradient on a Mastercycler® X50s (Eppendorf, Catalog # 6311000010). Room temperature controls were incubated for 1 h on the benchtop in 200-µL PCR tubes ...
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bioRxiv - Genomics 2020Quote: The ST slide with the tissue section was warmed to 37°C for 1 minute on a thermal incubator (Eppendorf Thermomixer Option C, Germany). The tissue was then covered with 4% formaldehyde (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were dissolved in 0.1% RapiGest and 500 mmol/L TEAB and then incubated at 850 rpm for 45 min at 37 °C (Eppendorf Comfort Thermomixer, Eppendorf, USA). Proteins were digested by trypsin (Trypsin Gold ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was rinsed with fresh HBSS and digested with 20 U/ml papain (#LS003126, Worthington Biochemical) (500 μl per one hippocampi Eppendorf, 1 ml for cortex) for 30 minutes on thermomixer at 37°C with gentle shaking (350 rpm) ...
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bioRxiv - Bioengineering 2023Quote: ... Fluorescence measurements were taken at day 1 (when the PCL-TMA scaffolds were removed from the Eppendorf tube after 24 hours of cell seeding) and on day 14 (each PCL-TMA scaffold was moved to a new 24 well plate to ensure only the cells adhered to the scaffold were quantified) ...