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Citations for Eppendorf :
1051 - 1098 of 1098 citations for 6 Amino 4 hydroxy 3 7 sulfo 4 4 sulfophenyl azo 1 naphthyl azo naphthalene 2 7 disulfonic acid sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Hydrolysis reactions were performed in the presence of 1% starch and 15.6 nM of free purified protein WT or H222Q in a final volume of 1 mL at 85 °C and 500 rpm in a thermoblock mixer (Eppendorf). Soluble starch (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA (1:30 dilution) was utilized for 30-cycle three-step PCR in Mastercycler Ep Gradient Auto thermal cycler (Eppendorf) using Q5® High-Fidelity 2× Master Mix (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Another 1 ml of Washing buffer B was added and transferred to 1.5 ml Protein lo-bind tubes (Eppendorf, 0030108116) with beads ...
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bioRxiv - Biophysics 2021Quote: Bacterial suspensions were incubated with different concentrations of AMPs in buffer for 1 hour at 37 °C (incubator Thermomixer C, Eppendorf, Germany). Reference samples (no AMPs ...
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The effect of hybridization on transposable element accumulation in an undomesticated fungal speciesbioRxiv - Genomics 2020Quote: ... 40 μl of cultures at 0.2 OD595 ml-1 were added to the plates using an Eppendorf epMotion 5075 liquid handling platform (Eppendorf, Hamburg, Germany). Replicates were positioned according to the predefined randomized scheme in side-by-side pairs ...
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bioRxiv - Bioengineering 2021Quote: ... CsupADH_17286 and HzeaADH7 were introduced into Agrobacterium tumefaciens GV3101 strain (MP90RK) by electroporation (1700 V mm-1, 5 ms, Eppendorf 2510). A viral silencing suppressor protein P19 was introduced into GV3101 strain as well in order to inhibit the host cells’ transgene silencing apparatus and extend transgene expression over a longer period of time with a higher degree of expression (Canto et al ...
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bioRxiv - Plant Biology 2020Quote: ... Bacterial pellets obtained from 1 mL of cell culture centrifuged at 13,000 rpm (Eppendorf 5424 Centrifuge with Fa-45-11 Rotor) were thoroughly washed three times with 1 mL fresh LB medium to remove residual antibiotics and were re-suspended in 100 µL liquid LB medium ...
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bioRxiv - Microbiology 2019Quote: ... similar reaction mixture of PCR except for 1×SYBR Green Real-time PCR Master Mix (Toyobo, Tokyo, Japan) was used and performed at Mastercycler EP realplex2 (Eppendorf, Germany). To estimate the copy numbers of aCPSF1 or RNAP captured genes ...
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bioRxiv - Biophysics 2021Quote: ... Concentration of the diluted solutions were confirmed by absorbance at 412 nm using an extinction coefficient ε412 of 36,000 M−1cm−1 (Eppendorf BioSpectrometer Kinetic). All dye solutions were encased in aluminum foil and stored at 4 °C for up to 7 d ...
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bioRxiv - Neuroscience 2022Quote: ... were diluted in BME (1:10) and locally injected into the outer SVZ by using a FemtoJet® 4i microinjector (Eppendorf). After 72 h ...
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bioRxiv - Microbiology 2022Quote: A lysate sample (1 ml) with 108 PFU/ml was centrifuged at 20,000 g (centrifuge 5430R, rotor FA-45-24-11HS; Eppendorf, Hamburg Germany) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the injection needle was loaded with the injection mixture calibrated using a microscale graticule to deliver 1-1.7nl per injection and each egg was injected (FemtoJet 4x, Eppendorf, Hamburg, Germany), once ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of MTBE was added and the solution was incubated for 1 hour at room temperature with continuous shaking at 600 rpm (Eppendorf, MixMate). Next ...
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bioRxiv - Synthetic Biology 2023Quote: ... then 1 mL aliquots were placed in triplicate in plastic-bottomed black 24-well plates (Eppendorf Cell Imaging Plates ref. 0030741005), and the plates were placed onto a custom made light plate apparatus (LPA)27 to control blue light illumination in each well (λ = 460 nm ...
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bioRxiv - Neuroscience 2023Quote: ... was added to lyophilized Aβ1-42 peptide to a final concentration of 1 mM and centrifuged in a speed vac (Eppendorf, Hamburg, Germany). The peptide pellet was re suspended in DMSO at a 5 mM concentration and sonicated in a 37⁰C water bath for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... by adding 50 μL of a 2 mg/mL stock solution and 25 μL of 50 mM CaCl2 and incubating for 1 h at 37 °C with agitation at 300 rpm in a Thermomixer comfort (Eppendorf, Germany). The cell wall material was pelleted and washed two times with 2 mL of ultrapure water (21000 g ...
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bioRxiv - Cell Biology 2023Quote: ... or at 37°C/5% CO2/1% O2 in a nitrogen-controlled hypoxic incubator (New Brunswick Galaxy 170R, Eppendorf, Hamburg, Germany). Prior to media changes ...
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bioRxiv - Biochemistry 2023Quote: ... was adjusted to 50 ng/μL with 50% ACN containing 0.1% TFA and dispensed in 10 μL each into normal tubes (1.5-mL safe-lock tube, CAT# 0030120086, Eppendorf, Hamburg; Germany), normal polypropylene (PP ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... A 1 ml sample of overnight suspension was centrifuged for five minutes at 8,000 rpm (Eppendorf MiniSpin F-45-12-11), the pellet resuspended in 1 ml PBS ...
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bioRxiv - Physiology 2024Quote: Protein samples were dissolved in 8 M urea in 50 mM ammonium bicarbonate (pH 7.8) by vortexing for 1 hour (Eppendorf ThermoMixer F1.5). 5 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2019Quote: ... and extension at 72°C for 1 min followed by final extension was conducted at 72°C for 5 min using a PCR minicycler (Eppendorf Ltd., Germany). After amplification ...
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bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were eluted with 60 μl of 80% acetonitrile/1% ammonia and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were dissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were eluted with 60 μl of elution buffer (80% acetonitrile/1% ammonia) and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were redissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Biophysics 2022Quote: ... The cultures were then induced with IPTG by adding 10 uL of 100 mM (final concentration approximately 1 mM) per well with an electric repeater pipette (Eppendorf, E4x series), and grown for another 4 h at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/ml of hygromycin and correct genome editing was confirmed by Sanger sequencing (Macrogen Europe ...
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bioRxiv - Developmental Biology 2020Quote: ... Microinjection of young adult hermaphrodite germlines was done using an inverted microinjection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). In cases where the sgRNA target site was not disrupted by sequence integration ...
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bioRxiv - Microbiology 2022Quote: ... 4.6×106 LMBs or NMBs were added and samples incubated at 37 °C for 1 hour on a thermomixer (60 rpm; Eppendorf, Hamburg, Germany). After this step ...
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bioRxiv - Biochemistry 2019Quote: ... 0.1% formic and mixed with the now washed Oligo R3 Resin and allowed to shake on a plate shaker (Eppendorf, Thermomixer Comfort) for 5 minutes at 800 RPM ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Systems Biology 2024Quote: ... are then added to the sample and incubated at 56°C for 1 hour at 1000 RPM (ThermoMixer C, Eppendorf, Hamburg, Germany). 37µl of 500mM chloroacetamide (50mM final concentration ...
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bioRxiv - Biochemistry 2024Quote: ... Constructs were expressed in 1 L LB/kanamycin medium and grown at 37 °C while shaking at 180 rpm (Eppendorf Innova S44i) until an OD600 of 0.6 was reached ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial mixtures were diluted 1:1000 into LB with or without 1% DMSO (v/v) and incubated standing at 37°C in 5% CO2 at atmospheric oxygen (normoxic; Eppendorf CellXpert incubator), 1% oxygen (hypoxic ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng corresponding pEcgRNA-N20 and 400 ng donor DNA were co-electrotransformed into 100 uL competent cells in 1 mm gap electroporation cuvette by applying 2.5 kV pulse in electroporator (Eppendorf™ Eporator™). After adding 900 uL of cold SOC medium ...
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bioRxiv - Biochemistry 2024Quote: ... A second gDNA purification was coupled with reverse transcription of RNA (1 μg) into cDNA with the SuperScript IV VILO ezDNase system (Thermo Fischer Scientific) using an Eppendorf Mastercycler (Eppendorf, Hamburg, Germany). cDNA samples were entered into a custom TaqMan low density array (Thermo Fischer Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Production of CjLPMO10AFL was achieved by fed-batch fermentation of the expression strain in a 1-liter fermenter (DASGIP® benchtop bioreactors for cell culture; Eppendorf, Hamburg, Germany), essentially as described previously (44) ...
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bioRxiv - Microbiology 2020Quote: ... printed arrays were moved to individual 1 mL centrifuge tubes containing 200 μL of M9 medium and melted at 55°C for 1 min in a thermomixer (Eppendorf ThermoMixer® C) shaking at 1000 rpm ...
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bioRxiv - Microbiology 2022Quote: ... Two-microliter aliquots of resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
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bioRxiv - Microbiology 2022Quote: ... Two μl aliquots of the resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
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bioRxiv - Bioengineering 2024Quote: ... 40 µL of clarified lysate was aliquoted into full-skirted PCR plates and incubated at the reported temperature for 1 h using a gradient on a Mastercycler® X50s (Eppendorf, Catalog # 6311000010). Room temperature controls were incubated for 1 h on the benchtop in 200-µL PCR tubes ...
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bioRxiv - Genomics 2020Quote: The ST slide with the tissue section was warmed to 37°C for 1 minute on a thermal incubator (Eppendorf Thermomixer Option C, Germany). The tissue was then covered with 4% formaldehyde (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was rinsed with fresh HBSS and digested with 20 U/ml papain (#LS003126, Worthington Biochemical) (500 μl per one hippocampi Eppendorf, 1 ml for cortex) for 30 minutes on thermomixer at 37°C with gentle shaking (350 rpm) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were dissolved in 0.1% RapiGest and 500 mmol/L TEAB and then incubated at 850 rpm for 45 min at 37 °C (Eppendorf Comfort Thermomixer, Eppendorf, USA). Proteins were digested by trypsin (Trypsin Gold ...
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bioRxiv - Bioengineering 2023Quote: ... Fluorescence measurements were taken at day 1 (when the PCL-TMA scaffolds were removed from the Eppendorf tube after 24 hours of cell seeding) and on day 14 (each PCL-TMA scaffold was moved to a new 24 well plate to ensure only the cells adhered to the scaffold were quantified) ...
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bioRxiv - Cell Biology 2024Quote: ... SCs were proliferated in SFGM on Cytodex- 1 microcarriers (Cytiva) in a 50c BioBLU single use vessel (Eppendorf; 37 °C, pH 7.2, 25 rpm) with a working volume of 40 L ...
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bioRxiv - Biochemistry 2024Quote: ... the samples received 50 µl 1 M NaOH (500 mM final concentration; dispensed with an insulin syringe by punching the lid of the Eppendorf, without opening the tube).
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bioRxiv - Bioengineering 2023Quote: ... Fluorescence measurements were taken at day 1 (when the PCL-TMA/PCL-TMA900 scaffolds were removed from the Eppendorf tube after 24 hours of cell seeding) and on day 14 (each scaffold was moved to a new 24 well plate to ensure only adherent cells were quantified) ...