Labshake search
Citations for Eppendorf :
951 - 1000 of 1088 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... This isolation method included a penultimate centrifugation step in Eppendorf polypropylene conical tubes (10,000 x g for 30 min at 4°C, in Eppendorf rotor F-34-6-38) that allowed the removal/isolation of larger microvesicles ...
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bioRxiv - Biochemistry 2023Quote: ... 12,000 rpm was applied for 30 minutes at 4 °C to obtain the supernatant and then evaporated to obtain a dry metabolite pellet (Eppendorf Concentrator plus, Hamburg, Germany). For the NMR experiments ...
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bioRxiv - Developmental Biology 2021Quote: ... the heads were cleaned with alcohol and a 1μl single cell suspension (5 x 106 cells/ml) was manually injected directly into the left cerebellar hemisphere using a glass microcapillary (Eppendorf, 5195 000.079) controlled with an Eppendorf CellTram Vario manual microinjector ...
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bioRxiv - Microbiology 2020Quote: ... The overnight culture was diluted in Mueller Hinton Broth (MHB) based on the OD600 to obtain 5 × 105 CFU/mL using a BioPhotometer D30 (Eppendorf, Hauppauge, NY). An OD600 of 1 was assumed to be equal to 8 × 108 CFU/mL.
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bioRxiv - Evolutionary Biology 2022Quote: ... We separated the tissue from the buffer and reproductive fluid by centrifuging at 4ºC for 5 min at 3500 rpm (Eppendorf Centrifuge 5702RH, USA), pipetted the supernatant from each tract region into microplate wells (CellStar 96 Well Cell Culture Plate ...
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bioRxiv - Microbiology 2020Quote: ... we centrifuged a 0.5-mL sample of culture in the exponential phase at 7000 rpm for 5 min in a Benchtop centrifuge (5424 Eppendorf, Hamburg, Germany). We washed the pelleted biomass 1 – 2 times and resuspended it with 50 µL 1x PBS from which we fixed 2 µL on solidified agarose (VWR ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial mixtures were diluted 1:1000 into LB with or without 1% DMSO (v/v) and incubated standing at 37°C in 5% CO2 at atmospheric oxygen (normoxic; Eppendorf CellXpert incubator), 1% oxygen (hypoxic ...
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bioRxiv - Cancer Biology 2023Quote: For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were maintained between 10% and 80% confluence and kept at 37 °C with 5% CO2 (8% CO2 for VPC cells) in a humidified CO2 incubator (Eppendorf, Enfield, CT). The viable cell density (VCD ...
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bioRxiv - Microbiology 2024Quote: ... one with the blood mixed to RPMI and the second with the gametocyte suspension were spun down at 2000rpm for 5 minutes in a temperature-controlled centrifuge at 37°C (Eppendorf 5702 R). RPMI supernatant was removed from both tubes with 3ml disposable Pasteur pipettes ...
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bioRxiv - Microbiology 2023Quote: ... CNW Technologies) using 80% ACN/5% NH4OH and dried at 45°C using a vacuum centrifuge (cat. no. 5305, Eppendorf, Hamburg, Germany). The peptide samples were resolved in 2% ACN/0.1FA for LC-MS analysis ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf, Hamburg, DE), to constitute 1 million cells per flow sample.
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bioRxiv - Developmental Biology 2023Quote: ... the gRNA:Cas9 ribonucleoprotein complex solution was incubated at 37°C for 5 min and then backfilled into 3 microinjection needles using an Eppendorf GELoader tip (Eppendorf, Cat# 022351656). After loading embryos into the embryo holder which covered with 12.5 ppt of salinity water with 0.0001% of methylene blue ...
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bioRxiv - Microbiology 2024Quote: ... The peptides were eluted in glass vials (CNW Technologies, cat#A3511040) using 80% ACN/5% NH4OH and dried at 45 °C using a vacuum centrifuge (Eppendorf, cat#5305). The peptide samples were resolved in 2% ACN/0.1FA for LC-MS analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged for 5 min at 16,000× g and the resulting supernatants were transferred to new microcentrifuge tubes before incubation at 99 °C for 5 min in a thermomixer (Eppendorf, Hamburg, Germany).
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bioRxiv - Microbiology 2024Quote: ... 1 ml of culture was centrifuged at 4000 x g for 5 min in a 1.5 ml reaction tube (Eppendorf AG, Hamburg, GER), and the resulting cell pellet was washed twice with 500 μl 1x PBS containing 1 g/L L(+)-ascorbic acid (AppliChem GmbH ...
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bioRxiv - Plant Biology 2024Quote: ... homogenized by vortexing and incubated at room temperature with Protein Assay Dye Reagent for 5 minutes before absorbance reading at 595 nm (Eppendorf Biophotometer D30).
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bioRxiv - Neuroscience 2020Quote: ... was added to a reagent-to-antibody molar ratio 10:1 in Protein LoBind tubes (Eppendorf, Hamburg, Germany), and the solutions were incubated for 1 h at room temperature under constant shaking at 500 rpm ...
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bioRxiv - Microbiology 2020Quote: ... and the other half was treated at 52°C for 10 minutes in a thermocycler (Eppendorf Mastercycler ProS) before being seeded in YPD ...
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bioRxiv - Microbiology 2020Quote: ... the Falcon tubes were centrifuged at 3500 rpm for 10 min in a swinging bucket centrifuge (Eppendorf, Germany) to separate the solid residue from the digested biomass ...
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bioRxiv - Immunology 2021Quote: ... 10 pl plasmid solution was injected with a pressure of 600 hPa into IVF-produced zygotes (FemtoJet, Eppendorf). The injected zygotes were cultured in PZM-3 medium at 39 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and the other half was treated at 55°C for 10 minutes in a thermocycler (Eppendorf Mastercycler ProS) before being transferred to YPD ...
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bioRxiv - Cell Biology 2023Quote: ... Resuspended cells were then plated in 10 cm dishes and incubated inside a cell culture incubator (Eppendorf, Germany) at 37°C with 5% CO2 for ninety minutes ...
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bioRxiv - Microbiology 2023Quote: ... Following a low speed-centrifugation for 10 min at 1,000 rpm in a R4702 centrifuge (Eppendorf, Hauppauge, NY) to remove rabbit Sf1Ep cells ...
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bioRxiv - Microbiology 2023Quote: ... The supernatants were then collected and centrifuged for 10 min at 12,000×g in a refrigerated centrifuge (Eppendorf) and filtered by using a 0.2 μm syringe filter ...
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bioRxiv - Immunology 2023Quote: ... the sample was briefly vortexed and incubated at 56°C for 10 minutes with a Thermomixer C (Eppendorf) at 1000 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... Protein fractions of interest were pooled and concentrated using an amicon of 10 kDa MWCO (Eppendorf 5804R, ∼3000g), before being loaded onto a size exclusion Superdex 75 HiLoad 16/600pg column (GE-Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein fractions of interest were pooled and concentrated using an amicon of 10 kDa MWCO (Eppendorf 5804R, ∼3000g) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Lipids were resuspended in 10 mM methanolic ammonium acetate and transferred to 96-well plates (Eppendorf Twintec 96). Mass spectrometry was performed on a Sciex QTRAP 6500+ mass spectrometer ...
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bioRxiv - Systems Biology 2023Quote: ... The protein extract from respective samples was spun down at 13,000 g for 10 mins and supernatant was collected in low adhesion 1.5 mL tubes (Eppendorf). We reduced the sample with 15 mM DTT (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then sonicated (Branson Digital Sonifier B450) at 25% power for 10 seconds and centrifuged (Eppendorf 5430R) at 12,000xg for 10 min at 4C ...
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bioRxiv - Cell Biology 2024Quote: ... was loaded with 10 μM of filtered folic acid solution and then connected to a FemtoJet microinjector (Eppendorf). The microinjector was operated in continuous injection mode with a compensation pressure of 15 hPa ...
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bioRxiv - Neuroscience 2021Quote: ... were incubated with ten-fold molar excess S-XL6 for 4 hours at 37 °C in protein low bind Eppendorf tubes using Eppendorf Thermomixer at 350 rpm (Eppendorf North America, Enfield, CT, USA). After incubation ...
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bioRxiv - Biophysics 2021Quote: ... Lipid extracts were resuspended in 60 μl 10mM ammonium acetate in methanol and diluted 1:4 in 96-well plates (Eppendorf twin.tec® 96; Sigma-Aldrich) prior to measurement of PC species ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of the enrichment was transferred to 5 L NMS medium in a 6-L fermenter controlled with a BioFlo® 120 Bioprocess Control Station (Eppendorf, Hamburg, Germany). Gas stream carrying a relatively low CH4 concentration (0.5% v/v in air ...
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bioRxiv - Biophysics 2020Quote: ... Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Microbiology 2024Quote: ... and 40 mL of the supernatant was added as the inoculum to 5 L medium prepared in a BioFlo® 120 bioreactor (Eppendorf, Hamburg, Germany). Air containing 0.5% or 10% (v/v ...
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bioRxiv - Pathology 2024Quote: ... Each plate was heated to 37°C for 60 min and 95°C for 5 min in a thermocycler (Eppendorf Mastercycler, Nexus SX5). Each well was then diluted ten times so as to obtain an estimated cDNA concentration of 50 ng/µL.
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bioRxiv - Cancer Biology 2024Quote: ... Aliquots of cells in αMEM were transferred to centrifuge tubes and centrifuged for 5 minutes at 900 rpm (Eppendorf 5804 R Benchtop Centrifuge). After removing the αMEM media ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were centrifuged at 16.000 x g for 10 min and 100 μL supernatant were transferred to a 1.5 mL low binding micro reaction tube (Eppendorf). For SH-group alkalization ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then analyzed by centrifugation at 13,000 rpm for 10 min (Eppendorf table top centrifuge, Eppendorf, Hamburg, Germany) at room temperature ...
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bioRxiv - Genomics 2020Quote: ... The lysates were then incubated at 37°C for 10 minutes at 1150 rpm shaking on a Thermomixer (Eppendorf). Lysates were cleared by centrifugation at 15,000g for 2 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... The second spin protocol applied in plasma quality measurements was 12,000×g RCF for 10 minutes with a commercial high-speed microcentrifuge (5417 R, Eppendorf), also based on cfDNA studies [15].
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bioRxiv - Biophysics 2020Quote: ... or 1 mM LY294,002 and 10 μg/ml Alexa594 were prepared in DB and loaded into Femtotips II (Eppendorf). The tip mounted on the micromanipulator (TransferMan 4r or TransferMan NK2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... samples were centrifugated at 15’000 g for 10 minutes and supernatants were dried at room temperature with a vacuum centrifuge (Eppendorf). Pellets were then resuspended in 1 mL solution containing 3% ACN and 0.1% of TFA and subsequently purified on Oasis HLB SPE columns (Waters ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting suspension was centrifuged 20,000×g for 10 minutes at room temperature in a table-top micro-centrifuge (Eppendorf), and 100 μl of the supernatant was subjected to immune precipitation using the anti-HA antiserum.
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bioRxiv - Molecular Biology 2020Quote: The resulting pellets were washed with ice-cold acetone (to neutralize the TCA and solvate lipids) and centrifuged at 20,000×g for 10 minutes in a table-top micro-centrifuge (Eppendorf). The obtained pellets were air-dried to rid them of residual acetone and solubilized with 120 μl Tris-SDS (10 mM Tris-HCl pH 7.5 and 2% SDS ...
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bioRxiv - Microbiology 2021Quote: ... cells equivalent to 1 OD600 were pelleted by centrifugation at 2,808 x g for 10 min (Eppendorf 5810 R) and the pellets were flash frozen ...
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bioRxiv - Immunology 2020Quote: ... Process development was performed in a scale-down models in a 10 L glass-vessel bioreactor (BioFlo 320, Eppendorf). Established process parameters were then scaled up to 120 L in a BioFlo Pro 150 stainless steel fermenter (Eppendorf) ...
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bioRxiv - Zoology 2020Quote: ... The tissue samples were separately placed in 400 µl of 10% Chelex solution in a 1.5 mL tube (Eppendorf) and crushed using a pestle (Eppendorf) ...