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Citations for Eppendorf :
51 - 100 of 100 citations for Small Volume Spectrophotometer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The concentrations of the protein were calculated by ultraviolet (UV) absorbance at 280 nm using a UV spectrophotometer (Eppendorf), and the calculated molar extinction coefficients of HHD1 ...
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bioRxiv - Microbiology 2019Quote: ... Aliquots of 120µL (∼100 worms) were laid into 96-deep-well culture plates (1 mL well volume, Eppendorf). 15µL of each bacterial suspension were added to the corresponding wells in a matrix-like format (11 species x 11 species) ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... Antibody binding was conducted in the volume of 50 µl on thermomixer for 96-well PCR plates (Eppendorf) in 4 °C cold room for 2 hours ...
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bioRxiv - Biochemistry 2021Quote: ... and the remaining volume of the sample was transferred to 1.5 mL Eppendorf protein LoBind tubes (Eppendorf, Dublin) followed by acidification with trifluoroacetic acid (TFA ...
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bioRxiv - Biophysics 2023Quote: ... MC samples were loaded on the lower plate with a volume of 210 μl with a micropipette (Eppendorf Research® plus 1000 μl ...
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bioRxiv - Developmental Biology 2023Quote: ... The selected material was placed in a minimal volume of PBS in 1.5 ml tubes (low binding, Eppendorf), snap-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Molecular Biology 2019Quote: ... After centrifugation the extract was transferred to a spectrophotometer cuvette and absorbance was measured at 647 nm and 664 nm using BioSpectrometer kinetic (Eppendorf) spectrophotometer against buffered acetone as blank ...
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bioRxiv - Cell Biology 2022Quote: ... Absorbance was measured at 600 nm after every 12 h intervals till 4 to 5 days using a UV-visible spectrophotometer (Eppendorf).
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bioRxiv - Microbiology 2021Quote: ... cells were cultured in a test tube at 30°C and optical density at 600 nm (OD600) was recorded using a spectrophotometer (Eppendorf). To obtain doubling time ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA concentration was measured in a 1 μl sample diluted 1:200 in nuclease-free water in a spectrophotometer (BioPhotometer, Eppendorf). cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad ...
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bioRxiv - Synthetic Biology 2020Quote: ... but the overnight grown culture was instead aliquoted to a 1.0 OD as read by the Biophotometer D30 tabletop spectrophotometer (Eppendorf, Inc.). These aliquots were diluted to 1:10 ratio with either neutral or acidified M9 media and antibiotic in the 96-well plate for a final volume of 200μL ...
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bioRxiv - Microbiology 2023Quote: ... spectrophotometer at room temperature and normalised to absorption values at 750 nm which were measured with a BioSpectrometer basic (Eppendorf).
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bioRxiv - Evolutionary Biology 2023Quote: ... the suspensions were centrifuged at 14000 rpm for 5 mins and the DNA released in the supernatant was measured using absorbance at 260 nm using a UV-visible spectrophotometer (Eppendorf). The amount of mineral-adsorbed DNA was estimated using the following equation: Adsorption isotherms were plotted as equilibrium DNA concentration on the x axis and adsorbed DNA concentration on the y axis ...
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bioRxiv - Plant Biology 2023Quote: ... Protein concentration in the supernatant was determined according to the Bradford Ultra method (Bradford, 1976) on a Bio-Spectrophotometer (Eppendorf) at 595 nm ...
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bioRxiv - Microbiology 2021Quote: ... A working volume of 5ml was used in each batch culture at 37°C on a rotary shaker (Eppendorf). The pH of the growth medium was set to 7 ± 0.05 ...
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bioRxiv - Bioengineering 2022Quote: ... Subsequent washes were performed at a volume of 5 mL in 5 mL Eppendorf tubes (Cat. No. 0030122321, Eppendorf) and pelleted with a compatible microcentrifuge (MC-24™ Touch ...
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bioRxiv - Systems Biology 2021Quote: ... 5 ml of working volume was used in the batch culture at 37°C on a rotary shaker (Eppendorf). The pH of the growth medium was set to 7 ± 0.05.
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bioRxiv - Developmental Biology 2022Quote: ... A volume of 1μL of siRNA was drawn from the bottom of the tube through a micro-sampler (Eppendorf). During microinjection ...
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bioRxiv - Immunology 2020Quote: ... and RNAse free water for a final volume of 13 µl in 0.2 ml PCR Tube Strips (732-0098, Eppendorf). The reaction tubes were transferred into a C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were transferred to a 15 mL conical vial and 100 μL of the culture was used to determine OD600nm on a Biophotometer plus spectrophotometer (Eppendorf, Germany). Cultures with an OD600nm > 1.0 were diluted with sterile water and re-read ...
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bioRxiv - Immunology 2021Quote: ... Equal volume of Mycobacteria tuberculosis H37RA-CFA was mixed with MOG/PBS in a 5 mL protein Lobind tube (Eppendorf), followed by emulsification in ice water through sonication ...
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bioRxiv - Biochemistry 2021Quote: ... The reactions were carried out in a final volume of 50 μL by incubation by shaking in a Thermomixer C (Eppendorf) at 750 rpm ...
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bioRxiv - Bioengineering 2021Quote: ... cerevisiae CEN.PK-113-7D were performed in a 1 l working volume of a stirred fermenter (DASGIP parallel bioreactor system, Eppendorf). Throughout the fermentations the pH was kept at 5.1 and the temperature was kept at 30 degrees Celsius ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and then normalized using a 12-channel electronic pipette with a volume range of 10 to 100µL (Eppendorf, Hamburg, Germany) to obtain the concentration required for genotyping.
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bioRxiv - Microbiology 2021Quote: ... and a volume corresponding to an OD600nm of one (109 cells) was pelleted at 14,000 x g for one minute (Eppendorf MiniSpin). 20 µl of supernatant was mixed with 50 µl BioLux Gaussia Luciferase Assay substrate (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... and a concentration of 5 µg / ml of the plasmid with GFP (Green Fluorescent Protein) were transferred to an electroporation cuvette (2 mm wide and 400 µl in volume (Eppendorf)) ...
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bioRxiv - Biochemistry 2019Quote: ... Varying volumes of viral supernatant were mixed with 5 × 106 to 1 × 107 log phase Jurkat cells in a final volume of 10 ml within a T-25 flask (Eppendorf) and subsequently cultured for 72 hours ...
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bioRxiv - Plant Biology 2019Quote: ... A volume of each protein extract corresponding to 16 mg protein was transferred into a new 5 ml LoBind tube (Eppendorf) containing Dynabeads MyOne Streptavidin C1 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: Single cell-stage fertilized wildtype embryos of AB line were injected with a mix of 150 pg Cas9 mRNA and 100 pg stat1a or stat1b sgRNA and (in 1 nL volume) using a Femtojet 4i pressure microinjector (Eppendorf) and a M3301R Manual Micromanipulator (WPI) ...
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bioRxiv - Synthetic Biology 2022Quote: ... as glucose acceptor and 0.1 mM UDP-Glucose as glucose donor in a final reaction volume of 1 mL for 20 min at 30 °C and 800 rpm using a Thermoshaker (Eppendorf). Crude extracts used to test individual assemblies were diluted to an exact protein concentration of 50 μg/mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... in 200 µl volumes grown at 37 °C with 200 rpm shaking in an Innova 42R Incubator (Eppendorf, United Kingdom) for 22 h growth periods (‘days’) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reaction was performed in a final volume of 60 μL for 60 min at 30°C under stirring (750 RPM, Thermomixer, Eppendorf). γS-ATP was removed by dialyzing the reaction mix against kinase buffer ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with both TAP amplifications in a final volume of 1 ml into 96 deep well plate (Eppendorf), and after 7 days of expression mAb-containing supernatants were harvested by centrifugation.
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bioRxiv - Biochemistry 2020Quote: ... The absorption maximum was analysed at 595 nm against a blank value (demineralized water) using an UV/Vis spectrophotometer (BioPhotometer plus, Eppendorf, Hamburg, Germany). The dissolving procedure and spectrophotometric measurements were each performed in triplicate and the protein solubility was calculated according to the formula described by Zielińska et al ...
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bioRxiv - Microbiology 2021Quote: ... Bacillus cereus group isolates’ inocula were prepared from overnight cultures (see “Bacterial cultures” section) by adjusting their concentration to 1-2 × 108 CFU/ml using a spectrophotometer (Eppendorf BioPhotometer 6131). A previously established OD-CFU standard curve was used to estimate the CFU/ml based on the OD reading ...
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bioRxiv - Biophysics 2022Quote: ... adapters and the cholesterol strands were determined by measuring the absorbance at 260 nm using a spectrophotometer (Eppendorf Model 6131, Hamburg, Germany) and the Beer-Lambert law using the extinction coefficients provided by IDT.
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bioRxiv - Genetics 2022Quote: ... End-point fungal growth was assessed by measuring the medium turbidity at a wavelength of 600 nm (OD600) on BioPhotometer Spectrophotometer UV/VIS (Eppendorf, Hamburg, Germany).
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bioRxiv - Microbiology 2021Quote: ... We resuspended the isolated genomic DNA from each method in a final 100 µl volume in 1.5 ml LoBind microfuge tube (Eppendorf, Hauppauge, NY). We sought to maximize read lengths by implementing best practices for handling HMW DNA throughout all the methods ...
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bioRxiv - Microbiology 2021Quote: ... Reaction mixes were separated into triplicate reactions (each with a volume of 16.7 μl) in a 384 well plate using an epMotion 5075 liquid handling robot (Eppendorf, Hamburg, Germany). The resulting 384 well plate was then transferred to a thermocycler (Eppendorf ...
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bioRxiv - Biophysics 2022Quote: ... standard micro reaction tubes with either 1.5 mL or 15 mL volumes can be placed in a heating/cooling block or in a shaker for such tubes (e.g. Eppendorf ThermoMix C) for prolonged sample integrity ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl of the sample was mixed with an equal volume of buffered phenol and 20 μl of 20% SDS in a 2 mL centrifuge tube (Eppendorf, Germany). After adding 0.5g of 2 mm zirconia beads (BioSpec Inc. ...
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bioRxiv - Biochemistry 2019Quote: ... Growth kinetics with cysteine treatment was monitored by withdrawing aliquots at different time points and measuring the turbidity (at 600 nm) using a spectrophotometer (Eppendorf Biophotometer plus, USA).
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bioRxiv - Genomics 2020Quote: ... 3X in Ribohyb buffer and resuspended in a final volume of 10 ul Ribohyb buffer in a non-stick tube (Eppendorf Protein LoBind). The 10 µl hybridization reactions were added to 10 µl of resuspended beads for a binding reaction volume of 20 µl ...
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bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
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A Polybasic Domain in aPKC Mediates Par6-Dependent Control of Membrane Targeting and Kinase ActivitybioRxiv - Cell Biology 2020Quote: ... Approximately 22.5×107 suspension-adapted Expi293F cells were diluted in Expi293 Expression Medium to the final volume of 76.5 ml and cultured in a 37°C shaker (Eppendorf New Brunswick S41i). 90µg of plasmid DNA was mixed with 240µl Expifectamine reagent into a total final volume of 9ml OPTI-MEM medium and was incubated for 25 min ...
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bioRxiv - Bioengineering 2019Quote: ... Reactions (35 µL final volume) were incubated in Reactions (25 µL, Axygen qPCR tube) were incubated in the Mastercycler® real-plex instrument (Eppendorf, Germany). Initially ...
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bioRxiv - Physiology 2023Quote: ... Microtubes were briefly vortexed and spun to collect volume at bottom of tube prior to incubation for 60 minutes at noted temperatures in a thermomixer (Eppendorf ThermoMixer C). Microtubes were immediately transferred and centrifuged at 20,000xg for 60 minutes at +25°C ...
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bioRxiv - Microbiology 2023Quote: ... We performed fragmentation of 4 µg DNA in a 46-µL elution volume in Covaris G-tubes by centrifuging at 4200–5000 rpm (Eppendorf 5424 centrifuge) for 90 s to achieve fragment sizes of ∼20 kb ...
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bioRxiv - Cell Biology 2021Quote: ... of the same volume as the RIPA buffer at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany). The digested sample was labelled as the RIPA-insoluble fraction (Ins) ...
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bioRxiv - Biochemistry 2021Quote: ... The molecular weight cut-off columns were rinsed by addition of equal volumes of Milli-Q water and the proteins were dried with a speed-vac (Eppendorf, Vacufuge Concentrator, Stevenage, UK).