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Citations for Eppendorf :
51 - 99 of 99 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... The sample was subsequently centrifuged for 1 h at 11,000 x g at 4°C in an Eppendorf tabletop centrifuge 5810R (Eppendorf). The phage pellet was resuspended in 400 μl DNAseI buffer (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was incubated at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany) to reduce viscosity ...
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bioRxiv - Cell Biology 2020Quote: ... The whole lane was excised from the gel and future cut into approximate 3 x 1 x 2 mm3 slices (L x W x H) and each slice were placed into a separate LoBind tubes (Eppendorf) for destaining ...
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bioRxiv - Cell Biology 2022Quote: ... Absorbance was measured at 600 nm after every 12 h intervals till 4 to 5 days using a UV-visible spectrophotometer (Eppendorf).
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bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
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bioRxiv - Plant Biology 2020Quote: ... Each insoluble fraction was digested by 0.5 μg chymotrypsin and 1 μg trypsin at 37°C for 20 h shaking at 600 rpm (Eppendorf tabletop). Digestion reactions were stopped using TFA to a final concentration of 0.5% ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 min-4 h at 76°C in a heat block (speed of 300 rpm; ThermoMixer F1.5, Eppendorf, Germany) (Wagner et al. ...
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bioRxiv - Immunology 2024Quote: ... This slurry was incubated for 1.0 h at 21 °C while shaking at 1,200 rpm on an Eppendorf shaker (Eppendorf, Hamburg, Germany). Excess of CCP4 peptide was removed by washing the beads four times with 1.0 ml PBS ...
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bioRxiv - Systems Biology 2023Quote: ... Saponified non-polar metabolites were obtained by modifying the protocol with the addition of 60% KOH (10X) in 50% methanol followed by an incubation at 42°C for 1 h in a thermomixer (300 rpm Eppendorf), instead of the 1 h rotation at RT ...
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bioRxiv - Genomics 2022Quote: ... The beads were then incubated 1 h with the anti-Myc antibody (9E10) at 4°C (Eppendorf ThermoMixer, 1,300 rpm), washed three times in PBS/0.1 % BSA and two times in lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred in a new tube and the cytosolic fraction was dried under vacuum for 2 h at 55°C in a Speedvac (Eppendorf). Pellets were then stored at 4°C ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were transferred to SMART Digest Trypsin digest resin in PCR strips and incubated 4 h at 70°C and 1400 rpm mixing (Thermomixer C with ThermoTop, Eppendorf). Cysteines were 1 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were hydrolyzed at 100 °C for 2 h under orbital shaking at 600 rpm (ThermoMixer C, Eppendorf, Hamburg, Germany). After cooling ...
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: The DNA scaffold was self-assembled by incubating 5 DNA oligonucleotides following a temperature gradient from 95 °C to 4 °C for 6 h in a thermocycler (Mastercycler® nexus X2, Eppendorf) in Tris-EDTA buffer (TE buffer ...
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bioRxiv - Microbiology 2020Quote: ... The cultures were grown in BHI broth for 24 h and were centrifuged at 3200 × g for 20 min at 4°C (Eppendorf, 5424R). The cell pellets were transported on ice for proteome analysis at The Mass Spectrometry Centre ...
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bioRxiv - Genomics 2021Quote: ... Samples were then incubated at 56 °C with shaking at 400 rpm for 16 h in a Thermomixer (Eppendorf 5355 000.011). The lysate (500 µl ...
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bioRxiv - Microbiology 2024Quote: ... The crude EV fractions were prepared by ultracentrifugation (45,000 rpm, 1 h, 4°C; S50A rotor, Himac Ultracentrifuge CS100GXII; Eppendorf Himac Technologies) of the thus prepared supernatants.
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bioRxiv - Bioengineering 2024Quote: ... BMP-2 solution was added to FN coated polymers and after 2 h incubation solution was aspirated and collected in Protein LoBind Tubes (Eppendorf™). Enzyme-linked immunosorbent assays (ELISA ...
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bioRxiv - Biochemistry 2024Quote: ... #33045-U) and incubated for 2 h at 60 °C at 400 rpm on a thermomixer (Eppendorf ThermoMixer C, Germany, #EP5382000023). Then ...
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bioRxiv - Microbiology 2023Quote: ... by adding 50 μL of a 2 mg/mL stock solution and 25 μL of 50 mM CaCl2 and incubating for 1 h at 37 °C with agitation at 300 rpm in a Thermomixer comfort (Eppendorf, Germany). The cell wall material was pelleted and washed two times with 2 mL of ultrapure water (21000 g ...
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bioRxiv - Biophysics 2023Quote: ... were coated with Expi293 cell produced RS2 at 4 µg/mL concentration in 1x PBS (60 µl/well) and incubated for 2 h at 25 °C under gentle shaking condition (300 rpm) on a thermomixer (Eppendorf, USA) and then plate was transferred to 4 °C cold room for overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... −3.6 D/V: −1.6 with 4 µl of transfection mix using a pulled glass capillary and a FemtoJet microinjector (Eppendorf). In utero electroporation was performed at day E15.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The HeLa cells were then washed thrice and cultured in DMEM media with FBS and appropriate antibiotics for additional 24-h in a CO2 incubator (Eppendorf Galaxy 170R) before harvesting for downstream processing.
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bioRxiv - Neuroscience 2021Quote: ... The cells stably expressing H-Luc-NLS were plated on a black flat glass bottom 96-well plates (Eppendorf; tissue culture treated). They were incubated with 1 μM MaPCahigh AM dyes and 0.04% Pluronic-F127 for at least 2 h in 100 μL at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 20 μL of mAb solution was added to the cells in the filter wells and the cells were incubated for 1 h at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). After the incubation ...
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bioRxiv - Immunology 2021Quote: ... The 80 µL aliquot was lyophilised for 4 h at 30 °C with the aid of a vacuum concentrator (Eppendorf Concentrator 5301) attached to a refrigerated condensation trap (Savant) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The fluorocarbon/ethanol mix was shaken at room temperature for 16 h with 1000 rpm using an Eppendorf ThermoMixer C (Eppendorf AG, Germany) to perform the liquid-liquid extraction ...
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bioRxiv - Biochemistry 2020Quote: ... Phaff 1956 (Komagataella phaffii Kurtzman)27 was cultivated in a New Brunswick BioFlo 310 fed-batch fermenter for 38 h (Eppendorf, Hamburg, Germany) with full control over the input variables in terms of glucose as carbon source (Cambridge Isotope laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... were also added to each tube and incubated at 37°C for 3 h in a thermomixer (Eppendorf Thermomixer C, Hamburg, Germany). After 3 h incubation ...
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bioRxiv - Neuroscience 2022Quote: ... Medium containing viral particles was harvested approximately 72 h later through low-speed centrifugation at 1.500 g in an Eppendorf centrifuge (Eppendorf 5810R, Hamburg, Germany) for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... and the device was left running for a minimum of 3 h while droplets were being collected in a 1.5 mL microcentrifuge tube (Eppendorf®, Hauppauge, NY).
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bioRxiv - Plant Biology 2024Quote: ... The samples were then incubated for 3 h at 37 °C with 600 rpm shaking on a thermomixer with a ThermoTop (Eppendorf, Hamburg, Germany) to reduce condensation into the caps of collection tubes ...
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bioRxiv - Neuroscience 2024Quote: ... The pellets were resuspended in 100 µl of extraction buffer and incubated at 37 °C for 3 h with shaking at 500 rpm (Eppendorf ThermoMixer C). The samples were then centrifuged at 20,000 g for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... bound proteins were eluted from the streptavidin beads with 50 µl of Laemmli-DTT sample buffer containing 500 nM D-Biotin at 98°C by shaking at 1,000 rpm in a ThermoMixer (Eppendorf™). For mass spectrometry analysis ...
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bioRxiv - Neuroscience 2023Quote: ... PFF oligomers were incubated with or without SVD-1a in PBS pH 7.4 for 3 d at 37 °C under quiescent condition in low retention Eppendorf tubes (Eppendorf, GE). Prior to injection ...
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bioRxiv - Biophysics 2020Quote: ... Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Cell Biology 2021Quote: ... of the same volume as the RIPA buffer at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany). The digested sample was labelled as the RIPA-insoluble fraction (Ins) ...
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bioRxiv - Molecular Biology 2023Quote: ... Zygotes were collected from mated females 21–22[h post-hCG injection and microinjected into the cytoplasm using Eppendorf 5242 microinjector (Eppendorf-Netheler-Hinz GmbH) and Eppendorf Femtotips II capillaries with the following CRISPR cocktail ...
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bioRxiv - Bioengineering 2024Quote: ... 40 µL of clarified lysate was aliquoted into full-skirted PCR plates and incubated at the reported temperature for 1 h using a gradient on a Mastercycler® X50s (Eppendorf, Catalog # 6311000010). Room temperature controls were incubated for 1 h on the benchtop in 200-µL PCR tubes ...
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bioRxiv - Cell Biology 2024Quote: ... the resuspended cilia were centrifuged at 16000 × g for 10 minutes at 4°C in a microfuge (Eppendorf, Centrifuge 5415 D), and the supernatant was removed ...
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bioRxiv - Microbiology 2020Quote: ... The presumptive cultures were grown in TSBYE broth for 24 h at 30°C and centrifuged at 4°C (Eppendorf, 5810R, Auckland, New Zealand) at 3,220 × g for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:1v/v mixture of 33% ammonium hydroxide and 40% methylamine in water for 3 h at 37 °C (Eppendorf ThermoMixer C, 1000 rpm). The suspension was filtered ...
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bioRxiv - Plant Biology 2024Quote: ... 800 µL of the total supernatant was dried in a speed vacuum device for 14 h at room temperature (Eppendorf Concentrator Plus, Hamburg, Germany).
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bioRxiv - Cell Biology 2021Quote: ... cells were recovered in 1 mL YPD (1% yeast extract, 2% Bacto peptone, 2% D-glucose) at 30°C in a ThermoMixer C (Eppendorf, Hamburg, Germany) for 3 hours (200 RPM ...
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bioRxiv - Cell Biology 2024Quote: ... The sample was placed on ice to incubate for 30 minutes before the demembraned flagella supernatant was removed after centrifugation at 16,000 × g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415 D). The pellet containing the axoneme was resuspended in 245 μl of Cilia Final Buffer (without trehalose) ...
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bioRxiv - Cell Biology 2024Quote: The cilia suspension was thawed on ice and then centrifuged at 16000 g and 4°C for 10 minutes in a microfuge in a refrigerated room (Eppendorf, Centrifuge 5415 D). The pellet was resuspended in 250 μl of ice-cold Cilia Final Buffer (50 mM HEPES at pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... with three ssDNA overhang strands on a bottom partially complementary to the sequence on the magnetic beads (mag2, Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Bioengineering 2024Quote: ... Cultures were further incubated overnight (900 RPM, 20 °C, 70 % humidity, 20 h) and harvested by centrifugation (Eppendorf 5810R; 3,220 x g, 4 °C, 15 min). The obtained cell pellet was resuspended with 150 µL buffer (50 mM Bicine ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with 500μl of D-PBS and centrifuged at 400xg for 10 minutes at room temperature (Eppendorf Centrifuge 5810R, rotor A-4-62). The supernatant was removed and the cell pellet resuspended in 50μl of D-PBS before being stained with CD20-PE (clone A1SB12 ...