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Citations for Eppendorf :
51 - 100 of 109 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Additional primary chondrifying cell cultures were inoculated onto the surface of 30-mm round cover slips (3×30-μL droplets) (Menzel-Gläser, Menzel GmbH, Braunschweig, Germany) placed in 35-mm Petri dishes (Eppendorf). Hyaline cartilage-specific ECM production in cultures from days 0 to 15 was qualitatively analysed using metachromatic staining ...
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bioRxiv - Molecular Biology 2023Quote: ... 30- μL droplets of the cell suspension were inoculated onto the surface of 30-mm round cover slips (3 × 30-μL droplets) (Menzel-Gläser) placed in 35-mm Petri dishes (Eppendorf). As described above ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was sonicated 3 times for 1 minute in a waterbath sonicator and incubated in a ThermoMixer (Eppendorf) for 30 minutes at 37°C and 500 rpm ...
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bioRxiv - Synthetic Biology 2024Quote: ... with TaqMan® Gene Expression Assay and run using the QuantStudio™ 3 Real-Time PCR System (Eppendorf, A28137) at standard curve mode ...
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bioRxiv - Plant Biology 2024Quote: ... was added to each tube and incubated at 50 °C at 850 rpm for 3 hours using a Thermomixer F2.0 (Eppendorf). A total of 150 µL of each sample was then transferred to vials in preparation for injection in the GC-MS ...
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bioRxiv - Genomics 2024Quote: ... Deyolked embryos were pelleted at 400g for 3 minutes using a swinging-bucket centrifuge (Eppendorf 5430R with swinging buckets). Supernatant was discarded by aspiration and the pellet was washed and resuspended with 500 µL of PBS + 10% FBS before being spun down again ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.003% Tricaine (ethyl-3-aminobenzoate-methanesulfonate) for 60 min at 31 °C in a Thermomixer (Eppendorf, Thermomixer R) set to 100 rpm ...
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bioRxiv - Microbiology 2021Quote: ... 0.5-1 mL culture sample was harvested by centrifugation for 3 min at 13806 rpm (Centrifuge 5424, FA-45-24-11, Eppendorf) and resuspended in 100-500 µL 10 mM NaOH depending on the size of the cell pellet ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 15-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C until further analysis ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and sonicated in chilled PBS (1 s on ...
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bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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bioRxiv - Cell Biology 2021Quote: ... were vortexed 3 min at room temperature followed by centrifugation at 4,500 x g for 5 min at 4°C (Eppendorf #5010R). The supernatant fluid was poured into a chilled 2 ml screw-capped tube ...
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bioRxiv - Biophysics 2022Quote: ... the DNA droplet-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). For the segregation of DNA droplets using enzymatic activity ...
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bioRxiv - Synthetic Biology 2022Quote: The concentration and quality of the plasmid solutions were determined by diluting 1 ul of plasmid solution in 4 ul of Tris EDTA buffer and loading 3 ul of the diluted solution on a μCuvette G1 (Eppendorf). Optical densities were measured at 260 nm and 280 nm using a BioSpectrophotometer and Fluorimeter (Eppendorf) ...
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bioRxiv - Biophysics 2022Quote: ... The aqueous solution was layered on top of the oil-surfactant mix in a volumetric ratio of 1:3 inside a microtube (Eppendorf). The tube was manually shaken for about 30 s until water-in-oil droplets formed ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 30 mycn mutant or WT zebrafish embryos at 3 dpf were transferred to 1.5mL Low binding microcentrifuge tubes (Eppendorf 022431021). Trypsin-EDTA solution (Beyotime ...
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bioRxiv - Microbiology 2021Quote: ... was performed with 0.5 µg of ligated DNA with oligonucleotides 6212 and 6420 in a standard PCR program with a 3 min extension time on a Mastercycler thermocycler (Eppendorf). Phusion DNA polymerase (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation reactions were carried out for the indicated proteins using rabbit reticulocyte lysate and SP cells at a final concentration of 3×106 cells/ml at 30°C for 40 min in a thermomixer (Eppendorf) set to 900 rpm ...
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bioRxiv - Biophysics 2020Quote: ... the DNA-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). Droplet formation was induced by manual shaking for about 4 s as described earlier.[26] For the oil-phase ...
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bioRxiv - Physiology 2020Quote: ... with 3 mM Tris(2-carboxyethyl)phosphine hydrochloride(TCEP-HCl) (Thermo Pierce) while shaking at 600 rpm in a thermomixer (Eppendorf). Samples were alkylated with 9 mM iodoacetamide (22 °C ...
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bioRxiv - Genomics 2024Quote: ... and washed in nuclease-free H2O before proceeding to MNase digestion with 1-2U of MNase for 3×106 cells that went on for 1-hour at 37C while shaking in a thermomixer (Eppendorf) at 550rcf ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transferred to a PCR-strip tube and heated for 3 min using a Mastercycler Nexus X2 thermal cycler (Eppendorf). After heating ...
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bioRxiv - Molecular Biology 2023Quote: ... the rehydrated gel pieces were overlaid with 30 μL of 0.01% ProteaseMAX in 50 mM ammonium bicarbonate and incubated at 37°C for 3 hours with shaking at 800 rpm (Thermomixer, Eppendorf). The digestion reaction was transferred to a fresh tube ...
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bioRxiv - Neuroscience 2023Quote: ... PFF oligomers were incubated with or without SVD-1a in PBS pH 7.4 for 3 d at 37 °C under quiescent condition in low retention Eppendorf tubes (Eppendorf, GE). Prior to injection ...
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bioRxiv - Microbiology 2023Quote: ... The beads were pelleted (600 x g, 3 min) and the supernatant transferred to a fresh low protein binding tube (Eppendorf). The beads were washed with 1 x 200 µL HPLC-grade water and the washes combined with the original supernatant ...
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bioRxiv - Bioengineering 2020Quote: The concentration of nitrogen in the media was monitored by taking 1 ml samples from the cultures at various time points and centrifuging them for 3 min at 16900 × g (benchtop 5430R centrifuge; Eppendorf, Germany). The supernatant was retained and the nitrogen concentration was determined using the method of Scheiner71 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were incubated with the dye and shaken for 20 minutes at room temperature in the dark, then washed by centrifugation (3 min, 8000 RPM, 28 °C (Eppendorf 5430R)) and resuspension in M2G three times ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were vigorously vortex-mixed for 3 min and centrifuged at 14,000 rpm for 10 min at 4 °C (Eppendorf 5804 R). The clear supernatant was then transferred to a separate vial ...
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bioRxiv - Plant Biology 2022Quote: ... annealing at 51 °C for 30 s and extension 72 °C for 30 s for 25 cycles by final extension 72 °C for 3 min and reaction hold at 4 °C in a thermocycler (Gradient thermocycler® Eppendorf). Amplified gene products were sequenced using the Sanger sequencing method ...
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bioRxiv - Genetics 2024Quote: ... the samples were washed 3 times with 500 μl Perm wash (centrifugation for 2 minutes at 1600 rpm (Eppendorf centrifuge 5415R)) ...
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bioRxiv - Microbiology 2024Quote: Sample were retrotranscribed in cDNA using 1 µL of RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted material was aliquoted into 3 samples in low binding polypropypene tubes (nominally 1.5 mL) and dried in a vacuum concentrator (Vacufuge® plus; Eppendorf™) at room temperature without additional heating for about 3h ...
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bioRxiv - Microbiology 2023Quote: ... All microinjection procedures were carried out on 3 day post-fertilization embryos with the help of FemtoJet Microinjector and PatchMan NP2 Micromanipulator (Eppendorf, Germany). Embryos were divided into three groups-mock ...
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bioRxiv - Neuroscience 2024Quote: ... 300 μL of flow-through from the initial amicon concentration step above (< 3 kDa MWCO) was derivatised (alkylated) with NEM 20 mM and incubated in a thermomixer (Eppendorf, UK) at 25oC for 45 min at 850 rpm in the dark ...
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bioRxiv - Microbiology 2024Quote: Samples were retrotranscribed in cDNA using 1 µL of extracted RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
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bioRxiv - Physiology 2024Quote: ... 3 mL of 60% ACN / 0.1% FA (v/v) was loaded and collected into low-binding polypropylene tubes (Eppendorf, Hamburg, Germany). Samples were dried out using a SpeedVac (Eppendorf ...
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bioRxiv - Neuroscience 2024Quote: ... They were then resuspended to a concentration of ∼800-3000 nuclei per uL across 3-4 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512) in NSB ...
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bioRxiv - Genomics 2024Quote: ... Worms were centrifuged at 500x g for 3 minutes using a swinging bucket rotor in a tabletop centrifuge (Eppendorf Centrifuge 5702R). To decrease bacterial populations ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were maintained in a humidified atmosphere of 5% CO2 and 37 °C and were passaged every 2-3 days into 10 cm polystyrene coated plates (Eppendorf; EP0030700112-300EA) upon reaching high density ...
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bioRxiv - Systems Biology 2021Quote: ... Total volume of 20 mL from each culture was transferred to a 50 mL Falcon® filled with ice and was immediately centrifuged at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). Next the supernatant was discarded and the cell pellet was snap frozen into liquid nitrogen and stored at -80 °C ...
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bioRxiv - Systems Biology 2021Quote: For the extraction of total proteome 10 mL of each culture were transferred into ice-cold 15 mL Falcon® tubes which were centrifuged immediately at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). The supernatant from the centrifugation was discarded and the cell pellets were washed once with 1 mL of cold PBS buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and samples were vortexed at 1200 rpm for 2-3 hours at room temperature (MixMate ®, Eppendorf South Pacific, Sydney, NSW Australia). Following this ...
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bioRxiv - Systems Biology 2022Quote: ... each SCN was cut into 3 pieces with a scalpel on a glass slide and transferred to a protein LoBind tube (Eppendorf, Hamburg, Germany) prefilled with 250 µL lysis buffer (100 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... were also added to each tube and incubated at 37°C for 3 h in a thermomixer (Eppendorf Thermomixer C, Hamburg, Germany). After 3 h incubation ...
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bioRxiv - Plant Biology 2024Quote: ... SDS at a 1:3 (w/v) ratio and extracted by shaking 1000 RPM 95 °C for using a tabletop shaker (Eppendorf ThermoMixer F2.0). Samples were then centrifuged at 20,000 xg for 10 min at room temperature and the supernatant retained in new tubes ...
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bioRxiv - Biophysics 2023Quote: ... and the device was left running for a minimum of 3 h while droplets were being collected in a 1.5 mL microcentrifuge tube (Eppendorf®, Hauppauge, NY).
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bioRxiv - Plant Biology 2023Quote: ... The primers were annealed at 95°C for 4 min followed by 70°C for 3 min in a thermocycler (Eppendorf, Hamburg, Germany). The reaction was allowed to cool slowly to room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... the gRNA:Cas9 ribonucleoprotein complex solution was incubated at 37°C for 5 min and then backfilled into 3 microinjection needles using an Eppendorf GELoader tip (Eppendorf, Cat# 022351656). After loading embryos into the embryo holder which covered with 12.5 ppt of salinity water with 0.0001% of methylene blue ...
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bioRxiv - Biophysics 2023Quote: ... Cell growth was quantified at intervals by measuring their optical density at regular intervals (as mentioned in Figure 3) using a Biophotometer® D30 (Eppendorf, Germany). The morphology of cells at different growth stages was determined by a variety of microscopic techniques (as described in the below sections).