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Citations for Eppendorf :
901 - 950 of 995 citations for 1 tert Butoxy 4 methyl 2 nitrobenzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... the DNA droplet-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). For the segregation of DNA droplets using enzymatic activity ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was spun at 800 g for 10 min (spin 1) to remove cell debris (Eppendorf Centrifuges 5430 with Eppendorf FA-45-48-11 30-spot 45-degree fixed angel rotor) ...
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bioRxiv - Biophysics 2022Quote: ... The aqueous solution was layered on top of the oil-surfactant mix in a volumetric ratio of 1:3 inside a microtube (Eppendorf). The tube was manually shaken for about 30 s until water-in-oil droplets formed ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were cleaned up with 2x 100 µl 99% ddH2O 1% TFA into 2x 100 µl 99% Isopropanol 1% TFA at 1200 xg at RT each using a table-top centrifuge (Eppendorf). Afterwards ...
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bioRxiv - Cell Biology 2022Quote: ... The fully dried gel slices were incubated with 1 µg trypsin per tube in 50 mM ammonium bicarbonate buffer at 37°C overnight on a ThermoMixer (Eppendorf). Digested peptides were extracted twice with 150 µl of 30% acetonitrile ...
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bioRxiv - Biochemistry 2020Quote: ... frozen leaves were extracted in 50 mM P buffer (pH 7.5) at a ratio of 1:8 (w/v) and centrifuged (Eppendorf 5417R) twice at 18,000g for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were digested with trypsin (1:50 trypsin:protein ratio) overnight at 37°C with light agitation (450 rpm on a Eppendorf thermomixer). Digestion was stopped by the addition of trifluoracetic acid (TFA ...
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bioRxiv - Neuroscience 2022Quote: At time of collection asteroids were transferred to a 1.5 mL Protein LoBind Eppendorf (Eppendorf cat#022-43-108-1) and allowed to settle ...
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bioRxiv - Molecular Biology 2021Quote: ... albeit the injected droplets were collected in fractions of ∼1000 cells (∼27 µl of loaded droplets) in 1 ml LoBind tubes (Eppendorf) pre-filled with 200 µl of mineral oil ...
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bioRxiv - Developmental Biology 2020Quote: ... Pairs of PPFs were isolated manually with forceps from the lateral body wall and from the underlying septum transversum and liver (Video 1) and then each pair placed in a single well in a 96-well plate (Eppendorf) containing 100 μL of media ...
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bioRxiv - Biochemistry 2020Quote: ... chloroform (4:2:1, v: v: v, containing 20 mM ammonium formate. The solutions were then placed into the wells of an Eppendorf twin-tec 96-well PCR plate ...
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bioRxiv - Cell Biology 2021Quote: ... 20 g/L tetracycline hydrochloride) was grown in batch mode in a 1 L fermenter (DASGIP 1L Bioreactor, Vaudaux Eppendorf) at pH 7.0 (+/-0.2 ...
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bioRxiv - Microbiology 2020Quote: ... This mixture was centrifuged at maximum speed for 1 min and the supernatant was transferred to a tube containing 300 μL chloroform and Heavy Phase Lock Gel (Eppendorf), inverted for 2 min and centrifuged at maximum speed for 5 min ...
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bioRxiv - Biochemistry 2021Quote: ... CD34-) (Supplementary Fig. 1) were sorted at single-cell resolution into a 384-well Eppendorf LoBind PCR plate (Eppendorf AG) containing 1 μl of lysis buffer (50 mM Triethylammonium bicarbonate (TEAB ...
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bioRxiv - Developmental Biology 2021Quote: ... were diluted in water (1:10) and injected into the periplasm of the embryos during cellularization using a FemtoJet express microinjector (Eppendorf). The embryos used in this experiment expressed Utr-Venus and Sqh-mCherry which allowed us to monitor cell shape and actomyosin dynamics ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM β-glycerol phosphate and 2.5 mM Na2H2P2O7).) and cells scraped and transferred into Protein Lo-bind tubes (Eppendorf). Samples were sonicated for 10 cycles (30s on & 40s off ...
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bioRxiv - Biophysics 2020Quote: ... the DNA-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). Droplet formation was induced by manual shaking for about 4 s as described earlier.[26] For the oil-phase ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL with 0.1% TFA.
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bioRxiv - Microbiology 2022Quote: Single cell-stage fertilized wildtype embryos of AB line were injected with a mix of 150 pg Cas9 mRNA and 100 pg stat1a or stat1b sgRNA and (in 1 nL volume) using a Femtojet 4i pressure microinjector (Eppendorf) and a M3301R Manual Micromanipulator (WPI) ...
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bioRxiv - Synthetic Biology 2022Quote: ... as glucose acceptor and 0.1 mM UDP-Glucose as glucose donor in a final reaction volume of 1 mL for 20 min at 30 °C and 800 rpm using a Thermoshaker (Eppendorf). Crude extracts used to test individual assemblies were diluted to an exact protein concentration of 50 μg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... and streptomycin (10 µg ml−1) at 37° C for 40 min with shaking at 800 rpm in a thermomixer (Eppendorf). Digested medullae were resuspended and the enzymatic reaction quenched by addition of 10 volumes cold CMF-HBSS ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with both TAP amplifications in a final volume of 1 ml into 96 deep well plate (Eppendorf), and after 7 days of expression mAb-containing supernatants were harvested by centrifugation.
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bioRxiv - Molecular Biology 2024Quote: ... was injected into the cytoplasm of 1-cell embryo in a droplet of M2 medium using a FemtoJet microinjector (Eppendorf) with constant flow settings ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 6 μL by 0.1% TFA.
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bioRxiv - Genomics 2024Quote: ... and washed in nuclease-free H2O before proceeding to MNase digestion with 1-2U of MNase for 3×106 cells that went on for 1-hour at 37C while shaking in a thermomixer (Eppendorf) at 550rcf ...
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bioRxiv - Molecular Biology 2024Quote: ... or 40 µM equimolar anti-Dux/anti-Obox4 ASO mixture with 100 ng.μL-1 codon-optimized Obox4 mRNA was microinjected into the male pronuclei of zygotes using a microinjector (catalog no. 5252000013, Eppendorf). For developmental monitoring ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). Peptide samples were then prepared for LC-MS/MS analysis by dilution to 5 μL using 0.1% TFA.
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bioRxiv - Systems Biology 2023Quote: ... Saponified non-polar metabolites were obtained by modifying the protocol with the addition of 60% KOH (10X) in 50% methanol followed by an incubation at 42°C for 1 h in a thermomixer (300 rpm Eppendorf), instead of the 1 h rotation at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL with 0.1% TFA.
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bioRxiv - Molecular Biology 2023Quote: ... Freshly laid zebrafish eggs were collected from breeding tanks and 1 nL solution mix was injected using a FemtoJet 4i (Eppendorf) into the yolk before the first cellular division.
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bioRxiv - Genetics 2023Quote: ... Samples were mixed at 850 rpm for 1 hour at room temperature on a 96 well MixMate plate mixer (Eppendorf). The beads were concentrated using a magnet and the supernatants removed ...
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bioRxiv - Developmental Biology 2023Quote: ... the DNA was eluted from the beads by incubating with 150μl elution buffer (100mM NaHCO3 and 1% SDS) for 20 mins at 65°C with vortexing using Eppendorf ThermoMixer C (Eppendorf). The supernatant was collected ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). They were then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA.
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bioRxiv - Developmental Biology 2023Quote: ... combined with 3 μg/μL NeuroD:Cre-IRES-GFP construct and 1 μg/μL NeuroD:IRES-GFP vector using a micro injector (Eppendorf Femto Jet). Plasmids were further electroporated into the neuronal progenitors adjacent to the ventricle by discharging five electric pulses (35 V ...
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bioRxiv - Cell Biology 2023Quote: ... and eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). They were then prepared for LC-MS3 analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Neuroscience 2023Quote: Neurons remained in medium and were incubated in ∼1% O2 (5% CO2, 37°C) for 360 min in a triple-gas incubator (Eppendorf) and then returned to normoxic conditions (21% O2 ...
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bioRxiv - Biochemistry 2024Quote: Hydrolysis reactions were performed in the presence of 1% starch and 15.6 nM of free purified protein WT or H222Q in a final volume of 1 mL at 85 °C and 500 rpm in a thermoblock mixer (Eppendorf). Soluble starch (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA (1:30 dilution) was utilized for 30-cycle three-step PCR in Mastercycler Ep Gradient Auto thermal cycler (Eppendorf) using Q5® High-Fidelity 2× Master Mix (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Another 1 ml of Washing buffer B was added and transferred to 1.5 ml Protein lo-bind tubes (Eppendorf, 0030108116) with beads ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in 1ml 1 M sodium chloride and boiled at 100 °C for 20 min in a heating block (ThermoStat plus, Eppendorf). The suspension was centrifuged at 10,000 x g for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... EVs-beads conjugates were then incubated with 15 uL EVs detection reagent (5 uL of CD9, CD63, CD81 detection reagents) for 1 hour at room temperature in Thermomixer orbital shaker (Eppendorf). Samples were washed with 500 uL MACSPlex buffer and centrifuged at 3000xg for 5 minutes at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... The monomer samples were then each concentrated to 1 mM and incubated at 37 °C and 200 rpm (orbital shaker, Eppendorf) for 3 weeks ...
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bioRxiv - Cell Biology 2024Quote: ... a mix of pre-polymerized mother filaments (formed from 1 μM 10 % AlexaFluor-568-labeled G-actin incubated in F-buffer in an Eppendorf tube for 2 hours ...
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bioRxiv - Biophysics 2021Quote: Bacterial suspensions were incubated with different concentrations of AMPs in buffer for 1 hour at 37 °C (incubator Thermomixer C, Eppendorf, Germany). Reference samples (no AMPs ...
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The effect of hybridization on transposable element accumulation in an undomesticated fungal speciesbioRxiv - Genomics 2020Quote: ... 40 μl of cultures at 0.2 OD595 ml-1 were added to the plates using an Eppendorf epMotion 5075 liquid handling platform (Eppendorf, Hamburg, Germany). Replicates were positioned according to the predefined randomized scheme in side-by-side pairs ...
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bioRxiv - Bioengineering 2021Quote: ... CsupADH_17286 and HzeaADH7 were introduced into Agrobacterium tumefaciens GV3101 strain (MP90RK) by electroporation (1700 V mm-1, 5 ms, Eppendorf 2510). A viral silencing suppressor protein P19 was introduced into GV3101 strain as well in order to inhibit the host cells’ transgene silencing apparatus and extend transgene expression over a longer period of time with a higher degree of expression (Canto et al ...
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bioRxiv - Plant Biology 2020Quote: ... Bacterial pellets obtained from 1 mL of cell culture centrifuged at 13,000 rpm (Eppendorf 5424 Centrifuge with Fa-45-11 Rotor) were thoroughly washed three times with 1 mL fresh LB medium to remove residual antibiotics and were re-suspended in 100 µL liquid LB medium ...
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bioRxiv - Biophysics 2021Quote: ... Concentration of the diluted solutions were confirmed by absorbance at 412 nm using an extinction coefficient ε412 of 36,000 M−1cm−1 (Eppendorf BioSpectrometer Kinetic). All dye solutions were encased in aluminum foil and stored at 4 °C for up to 7 d ...
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bioRxiv - Neuroscience 2022Quote: ... were diluted in BME (1:10) and locally injected into the outer SVZ by using a FemtoJet® 4i microinjector (Eppendorf). After 72 h ...