Labshake search
Citations for Eppendorf :
851 - 900 of 1071 citations for 4 1 1 2 3 3 3 Hexafluoropropoxy acetophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The samples were incubated on ice for 30 min before the demembraned cilia were centrifuged at 16,000g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415D). The supernatant was discarded ...
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bioRxiv - Microbiology 2021Quote: ... Serum was separated from blood by centrifugation at 8,000 RPM for 30 minutes at 4 °C in an Eppendorf 5424R centrifuge (Eppendorf, Enfield, CT). Serum was heat inactivated by incubating at 56 °C for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: 96 well plates were coated with immunized vaccine antigen and incubated for two hours at 25 °C (4 μg/mL, in 1xPBS, 50 μL/well) under constant shaking (300 rpm) on a MixMate thermomixer (Eppendorf, USA). ACE2-hFc protein coating was used as a control for antigen immobilization ...
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bioRxiv - Biophysics 2020Quote: 96 well plates were coated with vaccine antigen and incubated overnight at 25 °C (4 μg/mL in 1x PBS, 50 μl/well) under constant shaking (300 rpm) on a MixMate thermomixer (Eppendorf, USA). Ovalbumin (4 μg/mL in 1x PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... Solutions were then spun down at 13,000 RPM for 30 minutes at 4°C in a benchtop micro-centrifuge (Eppendorf Centrifuge 5424) to remove cells and other solid debris ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were centrifuged at 14k rpm for 15 minutes at 4°C and the aqueous phase was collected into clean 1.5 mL Eppendorf tubes (Eppendorf, Hauppauge, NY). 500 µL of ice-cold isopropanol was added to each tube ...
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bioRxiv - Microbiology 2020Quote: ... The cultures were grown in BHI broth for 24 h and were centrifuged at 3200 × g for 20 min at 4°C (Eppendorf, 5424R). The cell pellets were transported on ice for proteome analysis at The Mass Spectrometry Centre ...
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bioRxiv - Bioengineering 2020Quote: ... Cotton swabs containing simulated forensic samples were placed in 500 μL of 1X PBS and mixed at 4°C in a Thermomixer (Eppendorf, Germany) set at 1,000 rpm for approximately an hour ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was used to fractionate the cell lysate (at 4 °C for 30 min at 4,416 g, Eppendorf, Centrifuge 5804 R) and at 4 °C for 1 hour for ultracentrifugation (70,658 g ...
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bioRxiv - Neuroscience 2023Quote: ... The cell culture supernatant was collected and pre-cleared from cells and larger particles by centrifugation at 400 × g at 4 °C for 10 min (Eppendorf 5810R) and 10,000 × g at 4 °C for 30 min (Eppendorf 5910R) ...
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bioRxiv - Biophysics 2023Quote: ... Lysate and cell fragments were separated by centrifugation at 4 °C for 20 min at 12,000 rpm (Centrifuge 5418 R, Eppendorf, Hamburg, Germany). Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: The filled needle is positioned on a micromanipulator (Narishige MMO-4) and connected to a positive pressure pump (Eppendorf FemtoJet 4i). Control embryos were injected with Cas9 protein and Lap2b-GFP mRNA only.
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bioRxiv - Neuroscience 2022Quote: ... Blood samples were collected on ice and centrifuged within 1h at 4°C for 10min at 3000 RPM (Eppendorf, Centrifuge 5804R). Separated plasma was stored at -80°C until the protein assay was initiated.
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bioRxiv - Neuroscience 2022Quote: ... and incubated (gentle agitation, 20 minutes, 4°C) in EDTA solution (20 mM EDTA-PBS, Ca/Mg-free) in LoBind Protein tubes (Eppendorf 0030122216). The specimen was shaken ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were washed with cold 70% ethanol and centrifuged at 19,000 × g for 5 min at 4°C and air dried for 15 min in the Centrifugal Vacuum Concentrator 5301 (Eppendorf, USA). The quality of RNA was assessed using a NanoDrop (ND-1000 ...
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bioRxiv - Microbiology 2024Quote: ... Cells grown under photoautotrophic and dark heterotrophic conditions were collected using centrifugation (6,000 rpm, 10 min, 4°C; R10A2 rotor, Himac CR21F, Eppendorf Himac Technologies) and washed with a small amount of PBS ...
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bioRxiv - Microbiology 2024Quote: ... The stationary pre-cultures were then pooled and washed by centrifugation (5 min, 3,220 g and 4 °C) using a 5810 R swing-out centrifuge (Eppendorf, Hamburg, Germany). After centrifugation ...
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bioRxiv - Neuroscience 2022Quote: ... 3 times with 10” ON and 30” OFF intervals on 20% power on ice and cleared by centrifugation at 21,000 rpm for 20min at 4°C (Eppendorf 5424R Centrifuge). 50µg of supernatant was mixed with NuPAGE LDS Sample Buffer (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... in 5% glucose solution v/v prior to adding 2X solubilization buffer and incubation at 37 °C for 4 hrs under gentle orbital agitation (500 rpm on Eppendorf Thermomixer). The product was then analyzed by SEC as described earlier.
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bioRxiv - Developmental Biology 2022Quote: ... The filled needle is positioned on a micromanipulator (Narishige MMO-4) and connected to a positive pressure pump (Eppendorf FemtoJet 4i). Embryos are placed in FHM drops covered with mineral oil under Leica TL Led microscope ...
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bioRxiv - Cell Biology 2024Quote: ... the resuspended cilia were centrifuged at 16000 × g for 10 minutes at 4°C in a microfuge (Eppendorf, Centrifuge 5415 D), and the supernatant was removed ...
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bioRxiv - Physiology 2024Quote: ... plasma samples were thawed from storage at -80°C and centrifuged (5,000 x g, 10 minutes, 4 °C; Eppendorf 5427R with rotor FA-45-48-11; Eppendorf, Enfield, CT). Supernatants were transferred to 96-well plates and stored at -80°C until analysis ...
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bioRxiv - Genomics 2021Quote: ... snap-frozen in five aliquots (Safe-Lock cup DNA LoBind 2 ml PCR clean tubes, Eppendorf, #0030108078) and stored at -80 °C ...
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bioRxiv - Genomics 2021Quote: ... the tube was incubated at room temperature for 2 minutes on a thermomixer (Eppendorf Thermomixer C, #5382000023) set to 1250 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... and lysed by pipetting for total RNA isolation or collected in 2 ml safe-lock tubes (Eppendorf) in 1x PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Snap-Cap Microcentrifuge Biopur™ Safe-Lock™ or Safe-Lock Tubes 2 mL were from Eppendorf™ ...
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bioRxiv - Microbiology 2020Quote: ... 10 g NaCl) in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf) were used to express the I53-50A or I53-50B.4PT1 proteins grown ...
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bioRxiv - Physiology 2023Quote: ... for EVs isolation on cell culture flask T-75 (1.5 − 2 × 106/ per flask; Eppendorf, Hamburg, Germany) and for immunostaining on Cell Imaging Cover Glasses (0.1 × 106 / per chamber ...
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bioRxiv - Synthetic Biology 2024Quote: ... COVID_ssRNA & SARS-CoV-2 mimic) triggers were freshly prepared by serial dilution in DNA LoBind tubes (Eppendorf) using nuclease-free water ...
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bioRxiv - Genetics 2024Quote: ... Microinjection was performed using an Eppendorf TransferMan NK 2 and a FemtoJet 4i system (Eppendorf, Hamburg, Germany). The eggs used for microinjection were generated by mating female and male wild-type Dazao moths ...
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bioRxiv - Genomics 2024Quote: ... The resultant DNA was saved in a 2 mL DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C until library preparation ...
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bioRxiv - Genomics 2024Quote: ... The sheared DNA was transferred to a 2 ml DNA LoBind® Tube (Eppendorf Cat. No. 022431048) and temporary stored at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... The sheared DNA was collected with a 2 ml DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C until library preparation ...
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bioRxiv - Immunology 2022Quote: ... followed by pooling of the eluates and complete drying in 2 mL protein LoBind tubes (#0030108450, Eppendorf). For further purification ...
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bioRxiv - Immunology 2022Quote: ... 384 wells (the entire plate, including negative controls) were pooled in a single 2 ml tube (Eppendorf). Purification of cDNA was performed by adding 400 μl of cDNA in a 1:1 ratio with SeraMag SpeedBeads in 19% w/v PEG 8,000 in a 1.5 ml LoBind tube ...
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bioRxiv - Cell Biology 2022Quote: For esiRNA transfection 35,000 U2OS cells were seeded in 2 ml medium in 6-well plates (Eppendorf) the evening before transfection ...
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bioRxiv - Immunology 2024Quote: ... 10 g NaCl) in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf), were transformed with a I53-50B.4PT1-encoding plasmid ...
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bioRxiv - Immunology 2024Quote: ... The mixture was incubated at 37°C for 2 h while shaken with a Thermomixer comfort (Eppendorf) at a setting of 300 rpm ...
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bioRxiv - Bioengineering 2023Quote: ... Fluorescence measurements were taken at day 1 (when the PCL-TMA/PCL-TMA900 scaffolds were removed from the Eppendorf tube after 24 hours of cell seeding) and on day 14 (each scaffold was moved to a new 24 well plate to ensure only adherent cells were quantified) ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 30 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 45 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was spun down at 13600 rpm for 10 minutes at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysate was spun down at 13600 rpm for 10 min at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Immunology 2021Quote: ... The 80 µL aliquot was lyophilised for 4 h at 30 °C with the aid of a vacuum concentrator (Eppendorf Concentrator 5301) attached to a refrigerated condensation trap (Savant) ...
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bioRxiv - Biophysics 2020Quote: ... 200 ml of M9 minimal media were added to 4 mL of pre-inoculum and the cell growth was monitored using Biophotometer D30 (Eppendorf, Hamburg, Germany) until the growth rate was 0.8 (OD at 600 nm) ...
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bioRxiv - Microbiology 2022Quote: ... coli S17-1 that contained the shuttle- or suicide-vector construct at 3700 rpm for 10 min at room temperature (Centrifuge 5920 R, rotor S-4×1000, Eppendorf, Hamburg, Germany). We mixed the E ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were pelleted by centrifugation at approximately 250 × g for 5 min at 4 °C (Eppendorf 5804 R, Hamburg, Germany). After resuspension ...
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bioRxiv - Cell Biology 2021Quote: Live-cell microinjection experiments were performed using a FemtoJet® 4i microinjector in conjunction with an InjectMan® 4 micromanipulation device (Eppendorf). All microinjections were performed using pre-pulled Femtotips® injection capillaries (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... freshly laid embryos were aligned against nitrocellulose paper and injected using a quartz capillary needle, three-axis micromanipulator (Narishige, MMO-4) and microinjector (Eppendorf FemtoJet 4X).
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bioRxiv - Zoology 2023Quote: ... The homogenates were centrifuged for 10 minutes (860 g at 4 °C) to remove the solid particulates (5810R, Eppendorf centrifuge, Hamburg, Germany) and the supernatant was removed and stored at -80 °C for later analysis [19] ...