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Citations for Eppendorf :
701 - 750 of 849 citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The cells were incubated with the dye and shaken for 20 minutes at room temperature in the dark, then washed by centrifugation (3 min, 8000 RPM, 28 °C (Eppendorf 5430R)) and resuspension in M2G three times ...
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bioRxiv - Microbiology 2021Quote: ... resuspended at a final concentration of 1x106 cells/ml in minimal media in a 1.5 ml tube and grown at 30°C with 800 rpm in an Eppendorf Thermomixer (Eppendorf, Hamburg, Germany). Cell size was analyzed after 4 days ...
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bioRxiv - Cell Biology 2021Quote: ... Bacterial cultures for assay plates were grown in LB overnight shaking at 37°C to an OD600 of 1.0 (Eppendorf BioPhotometer D30) and seeded onto NGM plates ...
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bioRxiv - Bioengineering 2020Quote: ... and 58 nM dsDNA target were mixed in reaction buffer before incubation for 15 min at 95 °C in a thermocycler (Eppendorf, Germany). Following this high-temperature incubation step ...
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bioRxiv - Bioengineering 2020Quote: ... and 0.8 µM ssDNA target were mixed in reaction buffer and then incubated for 15 min at 95 °C in a thermocycler (Eppendorf, Germany). Following high-temperature incubation ...
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bioRxiv - Microbiology 2021Quote: ... an aliquot of the culture was removed for CFU determination by plate counting and the remainder was centrifuged at 4°C and 3,100 rpm (Eppendorf Centrifuge 5415R) for 10 min to generate cell-free supernatants ...
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bioRxiv - Neuroscience 2020Quote: ... For bulk PFF production approximately 500μL of 5mg/ml monomeric protein was added to a 2-ml Eppendorf tube and placed on a thermomixer-C (Eppendorf, USA) shaker and incubated at 37°C and 1000RPM for up to 144 hours ...
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bioRxiv - Microbiology 2020Quote: ... The flasks were kept at 28 °C for five days with orbital shaking at 150 rpm (Innova 44, Eppendorf AG, Germany). Induced cultures were centrifuged at 9000 rpm for 10 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plates were sealed with Parafilm and incubated for 6 days at 25°C while shaking at 200 rpm (New Brunswick I26/I26R shaker, Eppendorf). On day 6 the plates were observed under a dissection microscope and embryonic lethality ...
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bioRxiv - Neuroscience 2020Quote: ... 200 mM solution in water) was then added to the samples and incubated for 4 hours at 21°C in a ThermoMixer (Eppendorf AG). 4 µL of 1M IAA (iodoacetamide in water ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were incubated on ice for 30 min before the demembraned cilia were centrifuged at 16,000g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415D). The supernatant was discarded ...
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bioRxiv - Genetics 2022Quote: ... Yeast strains were manually inoculated into 400 µL of liquid SC -lys medium with G418 and grown overnight in 2 mL 96 well plates at 30.0 °C with 1000 rpm mixing using a MixMate (Eppendorf, Hamburg, Germany). The following morning ...
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bioRxiv - Microbiology 2021Quote: ... Serum was separated from blood by centrifugation at 8,000 RPM for 30 minutes at 4 °C in an Eppendorf 5424R centrifuge (Eppendorf, Enfield, CT). Serum was heat inactivated by incubating at 56 °C for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: 96 well plates were coated with immunized vaccine antigen and incubated for two hours at 25 °C (4 μg/mL, in 1xPBS, 50 μL/well) under constant shaking (300 rpm) on a MixMate thermomixer (Eppendorf, USA). ACE2-hFc protein coating was used as a control for antigen immobilization ...
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bioRxiv - Biophysics 2020Quote: 96 well plates were coated with vaccine antigen and incubated overnight at 25 °C (4 μg/mL in 1x PBS, 50 μl/well) under constant shaking (300 rpm) on a MixMate thermomixer (Eppendorf, USA). Ovalbumin (4 μg/mL in 1x PBS ...
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bioRxiv - Biophysics 2022Quote: ... standard micro reaction tubes with either 1.5 mL or 15 mL volumes can be placed in a heating/cooling block or in a shaker for such tubes (e.g. Eppendorf ThermoMix C) for prolonged sample integrity ...
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bioRxiv - Bioengineering 2019Quote: ... and 180 nM dsDNA target were mixed in reaction buffer before incubation for 15min at 95 °C in a thermocycler ((Eppendorf, Germany)) ...
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bioRxiv - Systems Biology 2019Quote: ... and the bacterial cells were harvested by centrifugation (swing-out rotor A-4-44, Eppendorf; 3220 g, 8 min, 30°C). The cell pellet was resuspended in the mineral medium and centrifuged again ...
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bioRxiv - Synthetic Biology 2020Quote: ... Solutions were then spun down at 13,000 RPM for 30 minutes at 4°C in a benchtop micro-centrifuge (Eppendorf Centrifuge 5424) to remove cells and other solid debris ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were centrifuged at 14k rpm for 15 minutes at 4°C and the aqueous phase was collected into clean 1.5 mL Eppendorf tubes (Eppendorf, Hauppauge, NY). 500 µL of ice-cold isopropanol was added to each tube ...
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bioRxiv - Microbiology 2020Quote: ... The cultures were grown in BHI broth for 24 h and were centrifuged at 3200 × g for 20 min at 4°C (Eppendorf, 5424R). The cell pellets were transported on ice for proteome analysis at The Mass Spectrometry Centre ...
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bioRxiv - Microbiology 2021Quote: ... the pre‐cultures were incubated at 30°C with a shaking speed of 250 rpm (Innova 44, Eppendorf New Brunswick, DE).
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bioRxiv - Bioengineering 2020Quote: ... Cotton swabs containing simulated forensic samples were placed in 500 μL of 1X PBS and mixed at 4°C in a Thermomixer (Eppendorf, Germany) set at 1,000 rpm for approximately an hour ...
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bioRxiv - Synthetic Biology 2019Quote: ... The transformants were inoculated into 500 μL of LB media with 15 μg/mL of Gentamicin and 50 μg/mL of Ampicillin and grown overnight at 30° C in a Thermomixer Comfort Eppendorf thermomixer (Eppendorf, Germany). 250 μL of overnight culture were transferred to 10 mL (dilution 1:40 ...
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bioRxiv - Microbiology 2020Quote: ... and 100 U/mL penicillin/streptomycin (Himedia, Mumbai India) under conditions of 5% CO2 and humidified air at 37°C (Eppendorf India).
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bioRxiv - Genomics 2021Quote: ... Samples were then incubated at 56 °C with shaking at 400 rpm for 16 h in a Thermomixer (Eppendorf 5355 000.011). The lysate (500 µl ...
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bioRxiv - Cell Biology 2019Quote: ... The resin was pelleted down by centrifugation at 4°C at 1258 g for 5 min (Eppendorf #A-4-81 rotor) and washed with 25 ml ice-cold binding buffer [10 mM imidazole ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were dislodged from a T75 flask and pelleted via centrifugation at 1300 rpm for 5 minutes at 20 °C (Eppendorf 5804R). The supernatant was aspirated ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were vigorously vortex-mixed for 3 min and centrifuged at 14,000 rpm for 10 min at 4 °C (Eppendorf 5804 R). The clear supernatant was then transferred to a separate vial ...
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bioRxiv - Biochemistry 2022Quote: ... 50 µl of 50 mM ammonium bicarbonate was added to the gel pieces and incubated with 16.6 µl of propionylation reagent (1:3 v/v propionic anhydride in acetonitrile) for 15 mins at 37°C with agitation in a thermomixer at 900 rpm (Eppendorf, UK). The supernatant was then removed and the derivatisation process was repeated ...
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bioRxiv - Microbiology 2022Quote: ... Twenty μL of supernatants were used as input controls and stored at −20°C in low-bind tubes (Eppendorf, Enfield, CT). For each sample ...
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bioRxiv - Neuroscience 2022Quote: ... 3 times with 10” ON and 30” OFF intervals on 20% power on ice and cleared by centrifugation at 21,000 rpm for 20min at 4°C (Eppendorf 5424R Centrifuge). 50µg of supernatant was mixed with NuPAGE LDS Sample Buffer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Blood samples were collected on ice and centrifuged within 1h at 4°C for 10min at 3000 RPM (Eppendorf, Centrifuge 5804R). Separated plasma was stored at -80°C until the protein assay was initiated.
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bioRxiv - Neuroscience 2022Quote: ... and incubated (gentle agitation, 20 minutes, 4°C) in EDTA solution (20 mM EDTA-PBS, Ca/Mg-free) in LoBind Protein tubes (Eppendorf 0030122216). The specimen was shaken ...
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bioRxiv - Immunology 2022Quote: ... bacterial stocks were grown at 30°C for 2 days to an OD600 of 0.4 approximately 6.3×106CFU/mL (Eppendorf, BioPhotometer; Hamburg, Germany). Bacteria were washed with sterile PBS and aspirated through a 27G needle against the side of the tube multiple times to break up bacterial clumps ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was used to fractionate the cell lysate (at 4 °C for 30 min at 4,416 g, Eppendorf, Centrifuge 5804 R) and at 4 °C for 1 hour for ultracentrifugation (70,658 g ...
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bioRxiv - Neuroscience 2023Quote: ... The cell culture supernatant was collected and pre-cleared from cells and larger particles by centrifugation at 400 × g at 4 °C for 10 min (Eppendorf 5810R) and 10,000 × g at 4 °C for 30 min (Eppendorf 5910R) ...
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bioRxiv - Biophysics 2023Quote: ... Lysate and cell fragments were separated by centrifugation at 4 °C for 20 min at 12,000 rpm (Centrifuge 5418 R, Eppendorf, Hamburg, Germany). Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... by adding 50 μL of a 2 mg/mL stock solution and 25 μL of 50 mM CaCl2 and incubating for 1 h at 37 °C with agitation at 300 rpm in a Thermomixer comfort (Eppendorf, Germany). The cell wall material was pelleted and washed two times with 2 mL of ultrapure water (21000 g ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were dissolved in 0.1% RapiGest and 500 mmol/L TEAB and then incubated at 850 rpm for 45 min at 37 °C (Eppendorf Comfort Thermomixer, Eppendorf, USA). Proteins were digested by trypsin (Trypsin Gold ...
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bioRxiv - Biochemistry 2022Quote: ... and incubated with Methoxamine hydrochloride (MeOX-HCl, 2%, 40 μl) at 60 °C for 2 hours at 400 rpm in a thermomixer (Eppendorf, USA). After adding N-methyl-N-(trimethylsilyl ...
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bioRxiv - Biochemistry 2023Quote: ... in 5% glucose solution v/v prior to adding 2X solubilization buffer and incubation at 37 °C for 4 hrs under gentle orbital agitation (500 rpm on Eppendorf Thermomixer). The product was then analyzed by SEC as described earlier.
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bioRxiv - Cell Biology 2023Quote: ... The sample was then concentrated on a 1.2 µm size meshed mixed cellulose ester membrane (Merk) and pelleted using centrifugation for 5 minutes at 1000G and 20°C with a swinging bucket centrifuge (Eppendorf 5427R).
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 1 hour at a temperature of 4°C with shaking at 600 rpm in a Thermomixer comfort (Eppendorf, Germany). Stained cells were washed with ice cold FACS buffer twice before resuspension in FACS buffer for analysis ...
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bioRxiv - Cell Biology 2023Quote: ... or at 37°C/5% CO2/1% O2 in a nitrogen-controlled hypoxic incubator (New Brunswick Galaxy 170R, Eppendorf, Hamburg, Germany). Prior to media changes ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted samples were aliquoted in 2 mL tubes and vaporized under vacuum at 40°C using SpeedVac (Eppendorf, Hamburg, Germany). The samples were resuspended and further diluted in sterile water before applying to MBA ...
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bioRxiv - Genetics 2023Quote: ... Plates were sealed with Parafilm and incubated for 6 days at 20°C while shaking at 200 rpm (New Brunswick I26/I26R shaker, Eppendorf). On day six the plates were observed under a dissection microscope and the survival and reproduction phenotypes were assessed in each condition ...
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bioRxiv - Plant Biology 2023Quote: ... was added to frozen cell pellets and incubated for 10 min at 65°C and 1000 rpm in a Thermomixer (Eppendorf, Germany). The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C ...
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bioRxiv - Biophysics 2023Quote: ... were coated with Expi293 cell produced RS2 at 4 µg/mL concentration in 1x PBS (60 µl/well) and incubated for 2 h at 25 °C under gentle shaking condition (300 rpm) on a thermomixer (Eppendorf, USA) and then plate was transferred to 4 °C cold room for overnight ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were kept at 37 °C and 5% CO2 in a humidified incubator (Galaxy 170 S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80% ...