Labshake search
Citations for Eppendorf :
651 - 700 of 1101 citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... 50 μL of the polymer solution were quickly added using a micropipette (Thermomixer comfort, Eppendorf, 1100 rpm, 21°C) to 450 μL of milliQ water containing a chosen concentration of sodium chloride ...
-
bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 4°C (3220 g for 5 min, A-4-81 rotor, Eppendorf, Enfield, CT) to remove intact cells and debris ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were then centrifuged at 13,000×g for 20 min at 4 °C using an F2402 rotor in a GS15R table-top centrifuge (Eppendorf). Pellets were air dried ...
-
bioRxiv - Microbiology 2022Quote: ... After centrifugation of the suspension (10 min, 8000 rpm, 10°C) using an Eppendorf Centrifuge 5415R (Eppendorf, Hamburg, Germany), the 3-NPH method was used for the quantitation of SCFAs (49) ...
-
bioRxiv - Neuroscience 2022Quote: ... Lysates were centrifuged at 4°C at 15,000 RPM for 15 minutes in a table top centrifuge (Eppendorf 5424R). The supernatant’s protein concentration was measured using Bradford’s assay (Bio-Rad #5000202) ...
-
bioRxiv - Immunology 2022Quote: ... Lungs were snipped into small pieces with scissors in a 1.7 mL eppendorf tube and incubated on a 37°C shaker for 30-45 min at 700 rpm on a ThermoMixer F1.5 (Eppendorf). The digestion reaction was quenched by adding FBS and EDTA to a final concentration of 5% FBS and 10 mM EDTA ...
-
bioRxiv - Molecular Biology 2023Quote: ... The precipitate was eluted from the beads by shaking at 450 RPM at 65°C in a thermomixer (Eppendorf) overnight.
-
bioRxiv - Genomics 2022Quote: ... The slide was then air dried and warmed at 37°C for 1.5 minutes in a Thermoblock (ThermMixer with Thermoblock, Eppendorf) with a heated lid (ThermoTop ...
-
bioRxiv - Microbiology 2023Quote: ... OD660 of 0.3-0.5 mid-log) were harvested by centrifugation (5,000 x g, 5 min at 4°C) (Eppendorf centrifuge 5430R). The supernatant was immediately discarded ...
-
bioRxiv - Microbiology 2023Quote: ... harvested by centrifugation (3,500 × g for 20 min at 4°C, Centrifuge 5702 R, Eppendorf North America, Framingham, MA), and suspended in an equivalent amount of 1 × PBS ...
-
bioRxiv - Microbiology 2023Quote: ... The precipitated gDNA was pelleted by centrifugation (21,000 x g, 10 min at 4°C) in a tabletop centrifuge (Model 5424, Eppendorf). The supernatant was discarded by decantation ...
-
bioRxiv - Genomics 2022Quote: ... The slide was air dried and then warmed at 37°C for 1.5 minutes in a Thermoblock (ThermMixer with Thermoblock, Eppendorf) with a heated lid (ThermoTop ...
-
bioRxiv - Systems Biology 2023Quote: The dried protein pellet was resuspended in in 30 µL of 8 M Urea dissolved in 100 mM Ammonium bicarbonate (ABC) (Fluka) pH 8.8 and incubated at 1000 rpm at 25 °C on a thermoshaker (Eppendorf). Urea concentration was lowered to 0.8 M by addition of 250 µL of 100 mM ABC ...
-
bioRxiv - Cell Biology 2023Quote: ... and kept at 37°C with 5% CO2 in an incubator (New Brunswick Galaxy 170 S, Eppendorf, Schönenbuch, Switzerland).
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were centrifuged at 10,000 rpm at 4°C for 10 min and supernatants were dried under vacuum centrifugation (Eppendorf) overnight ...
-
bioRxiv - Microbiology 2023Quote: ... The parasite solution was pelleted at 1,200 g at 4°C for 10 min using a chilled large tabletop centrifuge (Eppendorf). The resulting pellet underwent 3 washes with intracellular buffer ...
-
bioRxiv - Genomics 2023Quote: ... Beads were then resuspended in 200 μL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and incubated at 65 °C in a Thermomixer (Eppendorf) with interval mixing to dissociate antibodies ...
-
bioRxiv - Cell Biology 2023Quote: ... The incubated sample was centrifuged at 20,800 g for 15 min at 4 °C (Rotor F45-30-11, Eppendorf), and the resulting supernatant was analyzed by immunoblotting using various antibodies ...
-
bioRxiv - Cell Biology 2023Quote: ... fractions numbered from 9 to 22 were processed by centrifugation (20,800 g for 30 min at 4 °C, Rotor F45-30-11, Eppendorf), and the resulting pellet was further resuspended in 160 μL of alkaline carbonate buffer (100 mM Na2CO3 ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were washed in 20 mL of chilled phosphate-buffered saline (1X PBS, pH 7.4), and centrifuged (1500 ×g, 4 °C, 15 min) using the Centrifuge 5810R (Eppendorf). The cell pellet was subsequently resuspended in 30 mL of ice-cold RSB hypo buffer (10 mM NaCl ...
-
bioRxiv - Microbiology 2024Quote: ... the homogenate was centrifuged at 2500 rpm for 10 minutes at 4°C using a benchtop centrifuge (Eppendorf, USA), and the supernatant was collected.
-
bioRxiv - Plant Biology 2024Quote: ... Cultures were grown overnight at 37 °C and 225 rpm in a I2500 Incubator Shaker incubator (Eppendorf, Hamburg Germany). After 18 hours ...
-
bioRxiv - Plant Biology 2021Quote: ... 50 μl unprocessed xylem sap were transferred to a 2 mL reaction tube (Eppendorf) and 200 μL extraction medium (methanol ...
-
bioRxiv - Microbiology 2020Quote: ... The aqueous layer was transferred into a 2 ml phase lock gel tube (Eppendorf), 750 μl chloroform (Roth ...
-
bioRxiv - Systems Biology 2021Quote: ... Next the spheroplasted cells were centrifuged at 1500 rpm for 2 min (Eppendorf centrifuge), the supernatant was removed and the cells were resuspended in 400 µL of breaking buffer which contained 10 mM Tris ...
-
bioRxiv - Plant Biology 2020Quote: ... and these pieces were kept in separate 2 mL low protein binding tubes (Eppendorf). Afterwards ...
-
bioRxiv - Cancer Biology 2020Quote: ... Peptides were concentrated for 2 h in a vacuum concentrator (Eppendorf Vacuum Concentrator plus) at 30°C and diluted to 100 µL with H2O ...
-
bioRxiv - Genomics 2020Quote: ... Acceleration and deceleration were set to 2 across all centrifugations (Eppendorf centrifuge 5804, Eppendorf). Plasma was aliquoted per 1500 µL and stored at -80°C until further processing.
-
bioRxiv - Genomics 2020Quote: ... Acceleration and deceleration were set to 2 across all centrifugations (Eppendorf centrifuge 5804, Eppendorf). Plasma was aliquoted per 1500 µL and stored at -80°C until further processing.
-
bioRxiv - Neuroscience 2020Quote: ... The samples were then centrifuged at 1600 rpm for 2 min (Eppendorf 5415R, 200xg) and the supernatant aspirated before the tissue was incubated in a collagenase solution containing 2 mg/mL collagenase type II (CLS2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 15.76 g EDTA (pH 8)) in 2 mL Safe-Lock tubes (Eppendorf, Hamburg, Germany). The QIAamp DNA Mini Kit (QIAGEN ...
-
bioRxiv - Physiology 2022Quote: Sperm were concentrated by pipetting 2 mL sperm water into a microcentrifuge tube (Eppendorf), followed by centrifugation at 1500 x g for 5 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... The aqueous layer was transferred into a 2 ml phase lock gel tube (Eppendorf) and mixed with 750 μl chloroform (Roth ...
-
bioRxiv - Neuroscience 2023Quote: ... All lipid extractions were performed in 2 mL polypropylene LoBind safe-lock tubes (Eppendorf).
-
bioRxiv - Neuroscience 2023Quote: ... each half cortex was frozen in low-bind 2-ml Eppendorf tubes (Eppendorf, 0030108132) prior to addition of 500 μl of 0.32 M Sucrose Solution (0.32 M sucrose (SLS ...
-
bioRxiv - Immunology 2023Quote: ... Remaining bacteria were pelleted for 2 min at 16100× g (Eppendorf Centrifuge 5415 D) and supernatants containing the oligopeptide phage libraries were collected and stored at 4 °C.
-
bioRxiv - Microbiology 2024Quote: ... We transferred the resulting suspension to a 2 ml Safe-Lock tube (Eppendorf 0030123620) and mechanically disrupted the samples using a TissueLyser LT (Qiagen 85600 ...
-
bioRxiv - Biochemistry 2024Quote: ... The sample was collected in a 2 mL round bottom tube (Eppendorf, Hamburg, Germany) centrifuged for 5 mins ...
-
bioRxiv - Bioengineering 2024Quote: ... placed in individual nuclease-free 2 mL centrifuge tubes (DNA LoBind® Tubes, Eppendorf) and homogenized using a rotor-stator homogenizer (TissueMiser ...
-
bioRxiv - Cell Biology 2020Quote: ... we centrifuged the crude lysate at 500 g for 5 min at 4°C in a microfuge (Eppendorf, model 5424R) to remove cell debris ...
-
bioRxiv - Developmental Biology 2021Quote: ... Japan) followed by a 1-hr incubation at 25 °C with intermittent vortexing using a thermomixer (Eppendorf, Wesseling-Berzdorf, Germany). Aliquots were designated as the RIPA-insoluble fraction and subsequently mixed with LDS sample buffer and boiled at 95 °C for 3 min before being stocked frozen at -30 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 mM label was subsequently added and samples were incubated over night at 4°C and 350 rpm in a Thermomixer (Eppendorf). Excess label was removed by using a Sephadex G25 column (GE Healthcare Bio-Science AB ...
-
bioRxiv - Bioengineering 2022Quote: ... The lysates were clarified by centrifugation at 18,500g at 4 °C for 30 minutes using tabletop centrifuge (Eppendorf Centrifuge 5810R). Soluble protein was separated from cell debris and the supernatant was passed through 5 mL HisTrap columns (Cytiva ...
-
bioRxiv - Biochemistry 2022Quote: ... at a final concentration of 1 mM for 30 min at 30 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). The reaction was quenched with ammonium bicarbonate at a final concentration of 50 mM for 10 min at 30 °C and 650 rpm ...
-
bioRxiv - Neuroscience 2021Quote: ... Single EGFP+ cells were sorted on a BD Influx equipped with a 140 µm nozzle and a cooling unit with a sample temperature of 4°C and collected into a DNA LoBind tube (Eppendorf) containing aCSF with 0.5% BSA ...
-
bioRxiv - Molecular Biology 2021Quote: ... The samples were incubated on ice for 10 minutes and then at 37°C for 10 minutes at 700 rpm shaking on a Thermomixer (Eppendorf). Lysates were cleared by centrifugation at 15,000 g for 2 minutes ...
-
bioRxiv - Molecular Biology 2019Quote: ... Total RNA was eluted in 30 μL nuclease-free water and stored at −80°C in low-bind tubes (Eppendorf) until further analysis.
-
bioRxiv - Genomics 2021Quote: ... Nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
-
bioRxiv - Genetics 2020Quote: ... The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042, Eppendorf, Inc.) and the resultant supernatant fractions were stored at −80°C ...
-
bioRxiv - Genomics 2021Quote: ... nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...