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Citations for Eppendorf :
651 - 700 of 704 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 5μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Synthetic Biology 2021Quote: ... 40-120 μl of culture supernatant was dried in 1.5 ml microcentrifuge tubes with a rotational vacuum concentrator (Concentrator Plus, Eppendorf) and derivatised in 20 μl methoxyamine chloride (30 mg/ml in pyridine ...
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bioRxiv - Plant Biology 2021Quote: Seeds of tobacco (Nicotiana tabacum L. cv. SR1) or Arabidopsis Colombia were placed in a 1.7 mL micro-tube (Eppendorf) filled with sodium hypochlorite (0.5% active material ...
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bioRxiv - Neuroscience 2020Quote: ... washed twice with 160 µL PBS and nearly 40,000 cells per well transferred onto the membrane and dried with a plate thermomixer (Eppendorf) at 50 °C ...
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bioRxiv - Immunology 2020Quote: ... Process development was performed in a scale-down models in a 10 L glass-vessel bioreactor (BioFlo 320, Eppendorf). Established process parameters were then scaled up to 120 L in a BioFlo Pro 150 stainless steel fermenter (Eppendorf) ...
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bioRxiv - Immunology 2020Quote: ... Established process parameters were then scaled up to 120 L in a BioFlo Pro 150 stainless steel fermenter (Eppendorf). Phosphate depletion during the growth phase induced UF6b expression into inclusion bodies ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were eluted in 40μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1μl by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5μl by 0.1% TFA.
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bioRxiv - Molecular Biology 2023Quote: ... 66 Peptides were eluted in 40 μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μl by vacuum centrifugation (Concentrator 5301, Eppendorf). They were then prepared for LC-MS/MS analysis by diluting it to 5 μl by 0.1% TFA.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were eluted with freshly prepared 60 μl of 1.25 % v/v ammonium hydroxide (NH4OH)/80 % v/v ACN and dried using a SpeedVac centrifuge (Eppendorf). Dried peptides were resuspended in 6 μL buffer A (2 % v/v ACN/0.1 % v/v TFA) ...
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bioRxiv - Biophysics 2024Quote: ... Larvae of 3 dpf were mounted in 2.5 mm wide V-shaped agarose trenches and microinjected after 160 mg/L tricaine treatment using a mechanical micromanipulator (CellTram oil vario microinjector, 5176000.025, Eppendorf) with GBM cells expressing GFP ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of formic acid 1% were placed in each Millipore Microcon 30 MRCFOR030 Ultracel PL-30 before centrifugation at 14,500 rpm (Eppendorf 5424 centrifuge) for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The media was spun down in a centrifuge at 4°C for 10 min at 4400 g (Centrifuge 5920 R, Eppendorf Nordic, Hørsholm, Denmark) and the supernatant collected and stored at -80° for 1H NMR analysis ...
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bioRxiv - Microbiology 2021Quote: ... Development of ∼60 % cytopathic effect (CPE) led to harvesting and clarification of supernatants (3184 x g, 20 min, 4 °C, in an Eppendorf 5810 R centrifuge). Aliquots were then titred by plaque assay (see below) ...
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bioRxiv - Biochemistry 2022Quote: ... For PCMjMAT the protein was further purified by heating at 80 °C for 20 min followed by 30 min centrifugation at 18213 × g (Eppendorf centrifuge 5427 R). Protein concentrations were determined by UV/vis absorption with calculated extinction coefficients (ε280 ...
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bioRxiv - Microbiology 2024Quote: ... The depolymerization was performed in an autoclave (Equitron®, India) under the operating conditions of 40 min and 121 °C followed by centrifugation (Eppendorf 5810 R) at 8000 g for 20 mins ...
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bioRxiv - Cancer Biology 2024Quote: ... Aliquots of cells in αMEM were transferred to centrifuge tubes and centrifuged for 5 minutes at 900 rpm (Eppendorf 5804 R Benchtop Centrifuge). After removing the αMEM media ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were eluted in 40 μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μl by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). Samples were then prepared for LC-MS/MS analysis by diluting them to 5 μl with 0.1% TFA ...
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bioRxiv - Cell Biology 2020Quote: Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 5 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Bioengineering 2021Quote: ... cerevisiae CEN.PK-113-7D were performed in a 1 l working volume of a stirred fermenter (DASGIP parallel bioreactor system, Eppendorf). Throughout the fermentations the pH was kept at 5.1 and the temperature was kept at 30 degrees Celsius ...
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bioRxiv - Cancer Biology 2021Quote: ... All solution was removed and 100 μL of acetonitrile was added to each tube and incubated for 5 min at room temperature on a ThermoMixer (Eppendorf) at 700 rpm ...
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bioRxiv - Bioengineering 2021Quote: ... and 100μL of pre-warmed Mammocult was added to the center of each well using an epMotion 96 liquid handler (Eppendorf). To generate larger rings (maxi-rings ...
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bioRxiv - Systems Biology 2020Quote: ... A fixed volume of 100 μL cells was used to inoculate 50 mL preculture M9 +40 mM MOPS media with 4 g/L glucose which was grown overnight in a shake flask at 200 rpm in a 37°C (Eppendorf) incubator ...
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bioRxiv - Microbiology 2020Quote: ... Newly emerged EVV-1 free EvaBx adults were CO2 anaesthetized and injected with 0.5 μL of the virus-containing extract between two abdominal segments using a stereomicroscope with a Cell Tram Oil microinjector (Eppendorf). As negative control in order to exclude any procedural contamination ...
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bioRxiv - Immunology 2021Quote: RBD219-WT and RBD219-N1C1 were expressed at the 5 L scale using a Celligen 310 benchtop fermentation system (Eppendorf). For the RBD-WT ...
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bioRxiv - Microbiology 2022Quote: ... 500 ul of fixative containing cells were added on 12 mm glass coverslip kept on a 35mm petri plate (glass coverslips were precoated with Ploy-L-Lysine) and centrifuged in a swing bucket centrifuge (Eppendorf) for 10 min at 5000 g ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL with 0.1% TFA.
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM D-L methionine) for 1 h at 4° C with shaking at 1400 rpm in a benchtop thermomixer 22331(Eppendorf). The beads were washed twice with binding buffer without soybean trypsin inhibitor and suspended in 50 % v/v binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... after which 25 μL of the eluate was taken out and transferred to a clean new 1.5 mL DNA Lobind tube (Eppendorf, DE) (5 μL were left behind) ...
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bioRxiv - Cell Biology 2024Quote: ... The culture was maintained in a humidified atmosphere containing 5% CO2 at 37 L in a cell incubator (Eppendorf, Germany). The medium was changed daily ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 6 μL by 0.1% TFA.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). Peptide samples were then prepared for LC-MS/MS analysis by dilution to 5 μL using 0.1% TFA.
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL with 0.1% TFA.
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... Bound phosphopeptides were eluted 2 times with 30 μl elution buffer (40 % ACN, 15 % NH4OH (25 %, HPLC grade)) each and collected by centrifugation into clean PCR tubes (Eppendorf). Samples were concentrated in a SpeedVac for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... in LB (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) grown in a 10 L BioFlo 320 Fermenter (Eppendorf). At inoculation ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). They were then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA.
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bioRxiv - Cell Biology 2023Quote: ... and eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). They were then prepared for LC-MS3 analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of the reaction mixture were added to 30 μL BCA reagent solution in a 384-well PCR plate (Eppendorf twin.tec PCR MTP 384 ...
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bioRxiv - Microbiology 2021Quote: ... with 100 μl aliquot of the ultra-low gelling temperature agarose gel solution (6% in Mueller Hinton Broth II, cation-adjusted) to 85 °C in a Thermomixer (Eppendorf Thermomixer R Shaker, Eppendorf). Once the temperature was reached ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were washed with cold staining buffer (1× PBS, 0.2% BSA and 5 mM glucose) and spun down at 150 × g with low brake (Eppendorf Centrifuge 5810 R, Hamburg, Germany). The cell number was counted using a hemocytometer ...
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bioRxiv - Microbiology 2020Quote: Up-scale cultivation of the PmamDC45-trans-at-pks strain was done in a 10 L BioFlow® 120 Fermenter (Eppendorf) (34 ...
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bioRxiv - Genomics 2022Quote: ... 70μl of the released material from each well were collected and stored in a new 96-well PCR plate (Eppendorf, Germany). Library preparation ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were dissolved in 0.1% RapiGest and 500 mmol/L TEAB and then incubated at 850 rpm for 45 min at 37 °C (Eppendorf Comfort Thermomixer, Eppendorf, USA). Proteins were digested by trypsin (Trypsin Gold ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... at 0.2 g/L in E3 medium and injected with molecules diluted in PBS using a microinjector (FemtoJet, Eppendorf, Montesson, France) under a stereomicroscope ...
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bioRxiv - Cell Biology 2024Quote: ... and 800 µM or 3.2 mM L-cystine at 37 °C and 500 rpm using an Eppendorf ThermoMixer® (Eppendorf, Germany). Incubation times varied according to the reagent ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL was transferred to a PCR-clean polypropylene V bottom 96-well plate and covered with a silicone seal (Eppendorf, Mississauga, ON). Reactions were incubated at 29°C for 16 hours and final reactions stored at -20°C ...
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bioRxiv - Biochemistry 2020Quote: ... a 250 mL preculture was used to inoculate 3.75 L of 70% D2O minimal media in a BioFlo 310 bioreactor (Eppendorf, Hauppauge, NY, USA). Cells were grown at 37 °C with dry ...
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bioRxiv - Molecular Biology 2022Quote: ... 2.5 µL of 100 mM Dithiothreitol (DTT) was added to each sample and incubated at 60LJ C for 15 mins (Eppendorf, Thermomixer C) and alkylated using 2.5 µL of 200 mM iodoacetamide (IAM ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was incubated for 30 min on a rotating wheel at 4°C and centrifuged at 16 000 g for 20 min at 4°C (Eppendorf centrifuge 5417-R, Rotor F45-30-11). The supernatant was transferred to a fresh 1.5 ml Eppendorf tube and the pellet was discarded ...
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bioRxiv - Biochemistry 2021Quote: ... Unreacted dye was removed by two passages over an equilibrated Bio-spin® 6 column filled with Bio-gel P-6 media in labeling buffer and centrifuged in a tabletop centrifuge (Eppendorf 5810 R, rotor A-4-62) at 1500 rpm for 3 min ...