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Citations for Eppendorf :
651 - 700 of 939 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 200 µL of K562 cell lysates (2.5 mg/mL) was added to the beads and incubated at 4 °C on thermomixer (5382000023, Eppendorf) at 1,000 rpm for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... The protein samples were subsequently aliquoted and dried at 30°C under vacuum centrifugation (Eppendorf 5301 Vacufuge Concentrator System). After rehydration with distilled H2O ...
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bioRxiv - Biophysics 2024Quote: ... the protein was concentrated to 250μl using a 100kDa molecular weight cut-off filter (Amicon), centrifuged to pellet aggregates (10,000g, 10min, 4°C) (5418R, Eppendorf) and injected in a Superdex 200 10/300 gel filtration column (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... PFFs were generated by incubation at 37°C for 7 days with constant shaking at 1,000 rpm (Eppendorf Thermomixer). After ultracentrifugation for 1 h at 100,000 g ...
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bioRxiv - Biochemistry 2024Quote: ... heat- denatured FCS matrix was a supernatant obtained after spinning (15,000 g, 15 minutes, 4 °C) 100% FCS (200 uL; 1.7 mL Eppendorf tubes) heated at 85°C for 15 minutes in a Thermomixer Comfort heating block (Eppendorf ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.003% Tricaine (ethyl-3-aminobenzoate-methanesulfonate) for 60 min at 31 °C in a Thermomixer (Eppendorf, Thermomixer R) set to 100 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... we centrifuged the crude lysate at 500 g for 5 min at 4°C in a microfuge (Eppendorf, model 5424R) to remove cell debris ...
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bioRxiv - Developmental Biology 2021Quote: ... Japan) followed by a 1-hr incubation at 25 °C with intermittent vortexing using a thermomixer (Eppendorf, Wesseling-Berzdorf, Germany). Aliquots were designated as the RIPA-insoluble fraction and subsequently mixed with LDS sample buffer and boiled at 95 °C for 3 min before being stocked frozen at -30 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM label was subsequently added and samples were incubated over night at 4°C and 350 rpm in a Thermomixer (Eppendorf). Excess label was removed by using a Sephadex G25 column (GE Healthcare Bio-Science AB ...
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bioRxiv - Bioengineering 2022Quote: ... The lysates were clarified by centrifugation at 18,500g at 4 °C for 30 minutes using tabletop centrifuge (Eppendorf Centrifuge 5810R). Soluble protein was separated from cell debris and the supernatant was passed through 5 mL HisTrap columns (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... at a final concentration of 1 mM for 30 min at 30 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). The reaction was quenched with ammonium bicarbonate at a final concentration of 50 mM for 10 min at 30 °C and 650 rpm ...
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bioRxiv - Neuroscience 2021Quote: ... Single EGFP+ cells were sorted on a BD Influx equipped with a 140 µm nozzle and a cooling unit with a sample temperature of 4°C and collected into a DNA LoBind tube (Eppendorf) containing aCSF with 0.5% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were incubated on ice for 10 minutes and then at 37°C for 10 minutes at 700 rpm shaking on a Thermomixer (Eppendorf). Lysates were cleared by centrifugation at 15,000 g for 2 minutes ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
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bioRxiv - Genetics 2020Quote: ... The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042, Eppendorf, Inc.) and the resultant supernatant fractions were stored at −80°C ...
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bioRxiv - Genomics 2021Quote: ... nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... Relative amount of bacteria on seeded plates was measured by washing bacteria off plates with M9 buffer following a 24 hour temperature shift to 25°C and OD600 was measured using a BioPhotomer D30 (Eppendorf).
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bioRxiv - Biophysics 2021Quote: ... at a final ratio of 2.5 nmol DSS per 1 µg protein for 30 min at 37 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). After quenching by addition of ammonium bicarbonate to a final concentration of 50 mM and incubation for 10 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The reactions were carried out in a final volume of 50 μL by incubation by shaking in a Thermomixer C (Eppendorf) at 750 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were started by addition of 59.0 μM ubiquitin and 2 mM ATP then incubated at 37°C in a Mastercycler nexus (Eppendorf, Germany) thermocycler for 2 hrs unless otherwise stated ...
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bioRxiv - Biochemistry 2020Quote: ... The resin was pelleted down by centrifugation at 4°C (1258 g for 5 min, Eppendorf #A-4-81 rotor) and washed with ice-cold 50 mL Binding buffer composed of 10 mM imidazole (pH 7.4) ...
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bioRxiv - Neuroscience 2020Quote: ... 180 μL aliquots of solubilized GlyT1 cell membrane were dispensed in a 4°C-cooled 96-well PCR plate (Eppendorf) in triplicates ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was resuspended in SK buffer (LS buffer, 1% sarkosyl, 500mM NaCl) and solubilized at 22°C for 60 minutes using a ThermoMixer 5350 (Eppendorf) at 500rpm ...
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bioRxiv - Microbiology 2021Quote: ... The samples were immediately cooled on ice and centrifuged for 12 min at 4°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf). The cell pellets were stored at −20°C until RNA extraction.
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bioRxiv - Microbiology 2021Quote: ... The cells were transferred into anaerobic 50 mL reaction tubes inside the anaerobic chamber and harvested outside of the anaerobic chamber for 12 min at 4°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf). The supernatant was discarded inside the anaerobic chamber and the pellet was resuspended in fresh RCM medium to an OD600 of 5-10 ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transferred into the anaerobic chamber and harvested for 12 min at 25°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf) outside of the anaerobic chamber ...
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bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
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bioRxiv - Biochemistry 2021Quote: ... 33 mg of MFC tissue stored at −80°C was transferred to 1.5 mL LoBind Eppendorf tubes (Eppendorf, Cat# 022431081) and combined with 0.5 mL homogenization buffer consisting of 8 M urea ...
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bioRxiv - Biochemistry 2021Quote: ... at a molar ratio crosslinker to lysines of ∼2.7 for 30 min (except for the time-course experiments, see below) at 37°C shaking at 650 rpm in a Thermomixer (Eppendorf). Protein samples were quenched by addition of ammonium bicarbonate to a final concentration of 50 mM and either directly evaporated to dryness or after an additional centrifugation step for 60 min at 21 000 x g in order to separate the condensed from the dilute phase ...
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bioRxiv - Biochemistry 2020Quote: ... Cultures were grown with shaking at 200 rpm at 30 °C on an Innova 2300 platform shaker (Eppendorf, Hamburg, Germany) to maximal culture density ...
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bioRxiv - Bioengineering 2021Quote: ... a 96-well plate pre-filled with 20 μL pure water and sorted with designated cells per well was frozen on dry ice for 5 min and heated by ThermoMixer C (Eppendorf) at 95 °C for 10 min followed by spinning down at 3000 rpm for 1 min ...
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bioRxiv - Microbiology 2020Quote: Cells were concentrated from 10 ml of the enrichment cultures by centrifugation (4500×g, 20 min, 28°C) using a swing-bucket rotor (Eppendorf) and resuspended in approximately 200 µl of the supernatant ...
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bioRxiv - Plant Biology 2020Quote: ... Each insoluble fraction was digested by 0.5 μg chymotrypsin and 1 μg trypsin at 37°C for 20 h shaking at 600 rpm (Eppendorf tabletop). Digestion reactions were stopped using TFA to a final concentration of 0.5% ...
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bioRxiv - Plant Biology 2021Quote: ... samples were derivatized with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (Sigma-Aldrich, 1.0 mg per sample, pH 4.0, 60 min, 37 °C under continuous shaking in an Eppendorf thermomixer) and analysed using a UPLC-MS/MS equipped with an electrospray interface in positive mode (ACQUITY ...
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bioRxiv - Microbiology 2021Quote: ... The dialysis block was then incubated at 37°C for 6 hours with constant shaking at 400rpm (Thermomixer comfort, Eppendorf). After 6 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells were cultured in a test tube at 30°C and optical density at 600 nm (OD600) was recorded using a spectrophotometer (Eppendorf). To obtain doubling time ...
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bioRxiv - Systems Biology 2021Quote: ... Complementary sequences were annealed at equimolar concentrations by heating mixtures to 95 °C for 10 min in a thermal block (Eppendorf) followed by their cooling to room temperature (RT ...
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bioRxiv - Cancer Biology 2020Quote: ... at a concentration of 100μg/ml for 1 hr at 37°C in a heat block (Thermo mixer, Eppendorf, Germany). After incubation 10μg/ml of propidium iodide was added in dark for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... were vortexed 3 min at room temperature followed by centrifugation at 4,500 x g for 5 min at 4°C (Eppendorf #5010R). The supernatant fluid was poured into a chilled 2 ml screw-capped tube ...
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bioRxiv - Cell Biology 2021Quote: ... The resuspended cilia sample was centrifuged at 16,000g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415D). The supernatant was discarded ...
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bioRxiv - Biochemistry 2022Quote: ... or 1% sodium deoxycholate (SDC), incubated for 10 min at room temperature (U) or at 60°C (GnHCl, SDC) in a ThermoMixer (Eppendorf), and subsequently disrupted by 2 × 20” high intensity sonication cycles at 4°C in a BioRuptor (Diagenode) ...
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bioRxiv - Cell Biology 2022Quote: ... bound proteins were eluted from the streptavidin beads with 50 µl of Laemmli-DTT sample buffer containing 500 nM D-Biotin at 98°C by shaking at 1,000 rpm in a ThermoMixer (Eppendorf™). For mass spectrometry analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization between the target mRNA and reporter-capture probe pairs was performed for 18 hours at 65°C using Mastercycler Pro S Thermal Cycler (Eppendorf) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... For that 2.5x105 cells were incubated with HIV-1 antigens in PBS/2% FCS for the indicated period of times on a 37°C thermomixer (Eppendorf). Cells were then fixed with IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37°C and 5% CO2 in a humidified incubator (Galaxy 170S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
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bioRxiv - Biophysics 2022Quote: ... with and without 1,4 dithiothreitol (DTT) (10 mM) and agitated at 400 oscillations per minute (Eppendorf Thermomixer C, Eppendorf, Germany) for 48 h.
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bioRxiv - Bioengineering 2022Quote: ... tumors were minced using a razor and digested with 1mg/ml collagenase A and collagenase D and 0.4mg/ml DNase I in PBS at 37°C for 2h with rotation at 600rpm in a thermomixer compact (Eppendorf). 10mM EDTA was then added to stop the enzymatic reaction ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37 °C and 5% CO2 in a Galaxy 170S CO2 humidified incubator (Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
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bioRxiv - Biochemistry 2022Quote: ... Peptides purified via the SDB-RPS approach were dried at 45 °C in a centrifugal vacuum concentrator (Concentrator 5301, Eppendorf), resuspended in buffer A* (0.1 % [v/v] TFA ...
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bioRxiv - Cell Biology 2022Quote: ... then with 150 µl of 100% acetonitrile to the gel pieces for 15 min at 25°C while being agitated at 1400 rpm in a ThermoMixer (Eppendorf). Extracted peptides combined ...