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Citations for Eppendorf :
651 - 700 of 701 citations for 8 CHLORO 2 CHLOROMETHYL 6 TRIFLUOROMETHYL IMIDAZO 1 2 A PYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... were diluted in BME (1:10) and locally injected into the outer SVZ by using a FemtoJet® 4i microinjector (Eppendorf). After 72 h ...
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bioRxiv - Microbiology 2022Quote: A lysate sample (1 ml) with 108 PFU/ml was centrifuged at 20,000 g (centrifuge 5430R, rotor FA-45-24-11HS; Eppendorf, Hamburg Germany) for 2 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... and microbubbles were isolated by 4 centrifugation wash cycles at 40 relative centrifugation force for 1 min (Eppendorf 5804, Hamburg, Germany), where the infranatant was discarded and the microbubble concentrate was saved and resuspended.
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bioRxiv - Molecular Biology 2021Quote: ... the injection needle was loaded with the injection mixture calibrated using a microscale graticule to deliver 1-1.7nl per injection and each egg was injected (FemtoJet 4x, Eppendorf, Hamburg, Germany), once ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of MTBE was added and the solution was incubated for 1 hour at room temperature with continuous shaking at 600 rpm (Eppendorf, MixMate). Next ...
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bioRxiv - Microbiology 2024Quote: ... The crude EV fractions were prepared by ultracentrifugation (45,000 rpm, 1 h, 4°C; S50A rotor, Himac Ultracentrifuge CS100GXII; Eppendorf Himac Technologies) of the thus prepared supernatants.
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bioRxiv - Synthetic Biology 2023Quote: ... then 1 mL aliquots were placed in triplicate in plastic-bottomed black 24-well plates (Eppendorf Cell Imaging Plates ref. 0030741005), and the plates were placed onto a custom made light plate apparatus (LPA)27 to control blue light illumination in each well (λ = 460 nm ...
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bioRxiv - Neuroscience 2023Quote: ... was added to lyophilized Aβ1-42 peptide to a final concentration of 1 mM and centrifuged in a speed vac (Eppendorf, Hamburg, Germany). The peptide pellet was re suspended in DMSO at a 5 mM concentration and sonicated in a 37⁰C water bath for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... by adding 50 μL of a 2 mg/mL stock solution and 25 μL of 50 mM CaCl2 and incubating for 1 h at 37 °C with agitation at 300 rpm in a Thermomixer comfort (Eppendorf, Germany). The cell wall material was pelleted and washed two times with 2 mL of ultrapure water (21000 g ...
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bioRxiv - Biochemistry 2023Quote: ... The cellulase-LPMO synergy was assessed by performing reactions with crystalline Avicel (1 % w/v) in 50 mM sodium phosphate buffer (pH 6.0) using a thermomixer (Eppendorf, Hamburg, Germany) incubated at 30 °C with horizontal agitation (1000 rpm) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 1 hour at a temperature of 4°C with shaking at 600 rpm in a Thermomixer comfort (Eppendorf, Germany). Stained cells were washed with ice cold FACS buffer twice before resuspension in FACS buffer for analysis ...
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bioRxiv - Cell Biology 2023Quote: ... or at 37°C/5% CO2/1% O2 in a nitrogen-controlled hypoxic incubator (New Brunswick Galaxy 170R, Eppendorf, Hamburg, Germany). Prior to media changes ...
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bioRxiv - Biochemistry 2023Quote: ... was adjusted to 50 ng/μL with 50% ACN containing 0.1% TFA and dispensed in 10 μL each into normal tubes (1.5-mL safe-lock tube, CAT# 0030120086, Eppendorf, Hamburg; Germany), normal polypropylene (PP ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... A 1 ml sample of overnight suspension was centrifuged for five minutes at 8,000 rpm (Eppendorf MiniSpin F-45-12-11), the pellet resuspended in 1 ml PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The protoplasts were collected by centrifugation at 1700 rpm for 1 minute using a swing-bucket rotor (Eppendorf S-4-72), washed with 15 mL of ice-cold solution 3 (26.4 g of ammonium sulfate ...
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bioRxiv - Microbiology 2024Quote: ... An overnight culture of 1 ml was pelleted down by centrifugation ((10,000 rpm at 4°C for 10 min; Eppendorf centrifuge 5810R) and washed thrice with M9 minimal (M9M ...
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bioRxiv - Microbiology 2019Quote: ... and extension at 72°C for 1 min followed by final extension was conducted at 72°C for 5 min using a PCR minicycler (Eppendorf Ltd., Germany). After amplification ...
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bioRxiv - Immunology 2022Quote: ... 20 μL of mAb solution was added to the cells in the filter wells and the cells were incubated for 1 h at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). After the incubation ...
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bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were eluted with 60 μl of 80% acetonitrile/1% ammonia and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were dissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were eluted with 60 μl of elution buffer (80% acetonitrile/1% ammonia) and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were redissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Biophysics 2022Quote: ... The cultures were then induced with IPTG by adding 10 uL of 100 mM (final concentration approximately 1 mM) per well with an electric repeater pipette (Eppendorf, E4x series), and grown for another 4 h at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/ml of hygromycin and correct genome editing was confirmed by Sanger sequencing (Macrogen Europe ...
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bioRxiv - Developmental Biology 2020Quote: ... Microinjection of young adult hermaphrodite germlines was done using an inverted microinjection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). In cases where the sgRNA target site was not disrupted by sequence integration ...
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bioRxiv - Microbiology 2022Quote: ... 4.6×106 LMBs or NMBs were added and samples incubated at 37 °C for 1 hour on a thermomixer (60 rpm; Eppendorf, Hamburg, Germany). After this step ...
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bioRxiv - Biochemistry 2019Quote: ... 0.1% formic and mixed with the now washed Oligo R3 Resin and allowed to shake on a plate shaker (Eppendorf, Thermomixer Comfort) for 5 minutes at 800 RPM ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Systems Biology 2024Quote: ... are then added to the sample and incubated at 56°C for 1 hour at 1000 RPM (ThermoMixer C, Eppendorf, Hamburg, Germany). 37µl of 500mM chloroacetamide (50mM final concentration ...
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bioRxiv - Plant Biology 2024Quote: ... SDS at a 1:3 (w/v) ratio and extracted by shaking 1000 RPM 95 °C for using a tabletop shaker (Eppendorf ThermoMixer F2.0). Samples were then centrifuged at 20,000 xg for 10 min at room temperature and the supernatant retained in new tubes ...
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bioRxiv - Biochemistry 2024Quote: ... Constructs were expressed in 1 L LB/kanamycin medium and grown at 37 °C while shaking at 180 rpm (Eppendorf Innova S44i) until an OD600 of 0.6 was reached ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial mixtures were diluted 1:1000 into LB with or without 1% DMSO (v/v) and incubated standing at 37°C in 5% CO2 at atmospheric oxygen (normoxic; Eppendorf CellXpert incubator), 1% oxygen (hypoxic ...
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bioRxiv - Microbiology 2023Quote: Liquid samples in 1 ml were collected and centrifuged for 20 min at 21 130 × g at 4°C (Centrifuge 5424 R; Eppendorf, Hamburg, Germany). The supernatants were used for chemical analyses by applying external standards for calibration and quality control ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng corresponding pEcgRNA-N20 and 400 ng donor DNA were co-electrotransformed into 100 uL competent cells in 1 mm gap electroporation cuvette by applying 2.5 kV pulse in electroporator (Eppendorf™ Eporator™). After adding 900 uL of cold SOC medium ...
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bioRxiv - Biochemistry 2024Quote: ... A second gDNA purification was coupled with reverse transcription of RNA (1 μg) into cDNA with the SuperScript IV VILO ezDNase system (Thermo Fischer Scientific) using an Eppendorf Mastercycler (Eppendorf, Hamburg, Germany). cDNA samples were entered into a custom TaqMan low density array (Thermo Fischer Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Production of CjLPMO10AFL was achieved by fed-batch fermentation of the expression strain in a 1-liter fermenter (DASGIP® benchtop bioreactors for cell culture; Eppendorf, Hamburg, Germany), essentially as described previously (44) ...
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bioRxiv - Microbiology 2020Quote: ... printed arrays were moved to individual 1 mL centrifuge tubes containing 200 μL of M9 medium and melted at 55°C for 1 min in a thermomixer (Eppendorf ThermoMixer® C) shaking at 1000 rpm ...
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bioRxiv - Microbiology 2022Quote: ... Two-microliter aliquots of resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
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bioRxiv - Microbiology 2022Quote: ... Two μl aliquots of the resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
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bioRxiv - Cancer Biology 2023Quote: ... at 4°C for 1 hour and centrifuged at 4°C at 14200 rpm for 50 min using a refrigerated table-top centrifuge (Eppendorf Centrifuge, Hamburg, Germany). The cleared lysates were loaded on wells of 96-well single-use filter micro-plates with 3 µm glass fibers and 25 µm polyethylene membranes (Agilent ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then digested for 4 h at 37°C (1000 x rpm for 1 h, then 650 x rpm, Eppendorf ThermoMixer®C). Samples were then diluted 1:1 with milliQ water ...
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bioRxiv - Bioengineering 2024Quote: ... 40 µL of clarified lysate was aliquoted into full-skirted PCR plates and incubated at the reported temperature for 1 h using a gradient on a Mastercycler® X50s (Eppendorf, Catalog # 6311000010). Room temperature controls were incubated for 1 h on the benchtop in 200-µL PCR tubes ...
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bioRxiv - Genomics 2020Quote: The ST slide with the tissue section was warmed to 37°C for 1 minute on a thermal incubator (Eppendorf Thermomixer Option C, Germany). The tissue was then covered with 4% formaldehyde (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was rinsed with fresh HBSS and digested with 20 U/ml papain (#LS003126, Worthington Biochemical) (500 μl per one hippocampi Eppendorf, 1 ml for cortex) for 30 minutes on thermomixer at 37°C with gentle shaking (350 rpm) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were dissolved in 0.1% RapiGest and 500 mmol/L TEAB and then incubated at 850 rpm for 45 min at 37 °C (Eppendorf Comfort Thermomixer, Eppendorf, USA). Proteins were digested by trypsin (Trypsin Gold ...
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bioRxiv - Bioengineering 2023Quote: ... Fluorescence measurements were taken at day 1 (when the PCL-TMA scaffolds were removed from the Eppendorf tube after 24 hours of cell seeding) and on day 14 (each PCL-TMA scaffold was moved to a new 24 well plate to ensure only the cells adhered to the scaffold were quantified) ...
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bioRxiv - Cell Biology 2024Quote: ... SCs were proliferated in SFGM on Cytodex- 1 microcarriers (Cytiva) in a 50c BioBLU single use vessel (Eppendorf; 37 °C, pH 7.2, 25 rpm) with a working volume of 40 L ...
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bioRxiv - Biochemistry 2024Quote: ... the samples received 50 µl 1 M NaOH (500 mM final concentration; dispensed with an insulin syringe by punching the lid of the Eppendorf, without opening the tube).
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bioRxiv - Molecular Biology 2024Quote: ... 1:1v/v mixture of 33% ammonium hydroxide and 40% methylamine in water for 3 h at 37 °C (Eppendorf ThermoMixer C, 1000 rpm). The suspension was filtered ...
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bioRxiv - Biophysics 2021Quote: ... Lipid extracts were resuspended in 60 μl 10mM ammonium acetate in methanol and diluted 1:4 in 96-well plates (Eppendorf twin.tec® 96; Sigma-Aldrich) prior to measurement of PC species ...