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Citations for Eppendorf :
651 - 700 of 961 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... annealing at 51 °C for 30 s and extension 72 °C for 30 s for 25 cycles by final extension 72 °C for 3 min and reaction hold at 4 °C in a thermocycler (Gradient thermocycler® Eppendorf). Amplified gene products were sequenced using the Sanger sequencing method ...
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bioRxiv - Biochemistry 2023Quote: ... in 5% glucose solution v/v prior to adding 2X solubilization buffer and incubation at 37 °C for 4 hrs under gentle orbital agitation (500 rpm on Eppendorf Thermomixer). The product was then analyzed by SEC as described earlier.
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bioRxiv - Developmental Biology 2022Quote: ... The filled needle is positioned on a micromanipulator (Narishige MMO-4) and connected to a positive pressure pump (Eppendorf FemtoJet 4i). Embryos are placed in FHM drops covered with mineral oil under Leica TL Led microscope ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 1 hour at a temperature of 4°C with shaking at 600 rpm in a Thermomixer comfort (Eppendorf, Germany). Stained cells were washed with ice cold FACS buffer twice before resuspension in FACS buffer for analysis ...
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bioRxiv - Cell Biology 2024Quote: ... the resuspended cilia were centrifuged at 16000 × g for 10 minutes at 4°C in a microfuge (Eppendorf, Centrifuge 5415 D), and the supernatant was removed ...
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bioRxiv - Cell Biology 2024Quote: ... The protoplasts were collected by centrifugation at 1700 rpm for 1 minute using a swing-bucket rotor (Eppendorf S-4-72), washed with 15 mL of ice-cold solution 3 (26.4 g of ammonium sulfate ...
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bioRxiv - Neuroscience 2024Quote: ... They were then resuspended to a concentration of ∼800-3000 nuclei per uL across 3-4 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512) in NSB ...
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bioRxiv - Microbiology 2024Quote: ... An overnight culture of 1 ml was pelleted down by centrifugation ((10,000 rpm at 4°C for 10 min; Eppendorf centrifuge 5810R) and washed thrice with M9 minimal (M9M ...
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bioRxiv - Physiology 2024Quote: ... plasma samples were thawed from storage at -80°C and centrifuged (5,000 x g, 10 minutes, 4 °C; Eppendorf 5427R with rotor FA-45-48-11; Eppendorf, Enfield, CT). Supernatants were transferred to 96-well plates and stored at -80°C until analysis ...
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bioRxiv - Systems Biology 2021Quote: ... 5 ml of working volume was used in the batch culture at 37°C on a rotary shaker (Eppendorf). The pH of the growth medium was set to 7 ± 0.05.
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bioRxiv - Genomics 2022Quote: ... Multiple 20µm tissue curls were cut per tissue and collected in pre-cooled 5 ml tubes (Eppendorf, Hamburg, Germany) ensuring no thawing while transferring and stored on dry ice until processing ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). All used cell lines were confirmed to be mycoplasma free by using MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Genetics 2021Quote: ... A study pool quality control (SPQC) sample was prepared by combining 5 µL of supernatant from each sample into a 1.5 mL tube (Eppendorf). Stable Isotope Labeled (SIL ...
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bioRxiv - Cell Biology 2021Quote: ... spun at 1,000 x g at −9°C for 5 minutes then aliquoted into lo-bind 2ml centrifuge tubes (Eppendorf) and snap frozen in liquid nitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Bound phosphopeptides were then eluted 3 times with 100 µL ammonium hydroxide (5% v/v) into 1.5mL Lo-Bind tubes (Eppendorf). These were then frozen and lyophilized ...
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bioRxiv - Microbiology 2022Quote: Sheep or horse erythrocytes were washed three times with sterile PBS (pH=7.4) at 300⍰×⍰g for 5⍰min in a refrigerated centrifuge (Eppendorf). These washed erythrocytes suspension was lysed with 0.1% saponin and the concentration of oxy-hemoglobin in this preparation was determined using the QuantiChrom™ Hemoglobin Assay Kit (BioAssay Systems) ...
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bioRxiv - Microbiology 2022Quote: ... supernatants containing planktonic bacteria were removed from cultures and bacteria were pelleted down by centrifugation at 13,000 rpm x 5 min in a refrigerated micro-centrifuge (Eppendorf). In another set of experiments ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae strain to 5 mL of YPD media and incubating in an orbital shaker incubator (Innova 42, Eppendorf, UK) at 30 °C and 250 rpm overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and kept at 37°C with 5% CO2 in an incubator (New Brunswick Galaxy 170 S, Eppendorf, Schönenbuch, Switzerland).
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bioRxiv - Biophysics 2024Quote: ... to a final concentration of 200 nM PCt and 0.1-8 M GdmCl in 5 ml Protein LoBind tubes (Eppendorf). To avoid time-dependent photobleaching ...
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bioRxiv - Genomics 2021Quote: ... snap-frozen in five aliquots (Safe-Lock cup DNA LoBind 2 ml PCR clean tubes, Eppendorf, #0030108078) and stored at -80 °C ...
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bioRxiv - Genomics 2021Quote: ... the tube was incubated at room temperature for 2 minutes on a thermomixer (Eppendorf Thermomixer C, #5382000023) set to 1250 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... and lysed by pipetting for total RNA isolation or collected in 2 ml safe-lock tubes (Eppendorf) in 1x PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Snap-Cap Microcentrifuge Biopur™ Safe-Lock™ or Safe-Lock Tubes 2 mL were from Eppendorf™ ...
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bioRxiv - Microbiology 2020Quote: ... 10 g NaCl) in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf) were used to express the I53-50A or I53-50B.4PT1 proteins grown ...
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bioRxiv - Physiology 2023Quote: ... for EVs isolation on cell culture flask T-75 (1.5 − 2 × 106/ per flask; Eppendorf, Hamburg, Germany) and for immunostaining on Cell Imaging Cover Glasses (0.1 × 106 / per chamber ...
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bioRxiv - Synthetic Biology 2024Quote: ... COVID_ssRNA & SARS-CoV-2 mimic) triggers were freshly prepared by serial dilution in DNA LoBind tubes (Eppendorf) using nuclease-free water ...
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bioRxiv - Genetics 2024Quote: ... Microinjection was performed using an Eppendorf TransferMan NK 2 and a FemtoJet 4i system (Eppendorf, Hamburg, Germany). The eggs used for microinjection were generated by mating female and male wild-type Dazao moths ...
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bioRxiv - Genomics 2024Quote: ... The resultant DNA was saved in a 2 mL DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C until library preparation ...
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bioRxiv - Genomics 2024Quote: ... The sheared DNA was transferred to a 2 ml DNA LoBind® Tube (Eppendorf Cat. No. 022431048) and temporary stored at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... The sheared DNA was collected with a 2 ml DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C until library preparation ...
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bioRxiv - Immunology 2022Quote: ... followed by pooling of the eluates and complete drying in 2 mL protein LoBind tubes (#0030108450, Eppendorf). For further purification ...
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bioRxiv - Immunology 2022Quote: ... 384 wells (the entire plate, including negative controls) were pooled in a single 2 ml tube (Eppendorf). Purification of cDNA was performed by adding 400 μl of cDNA in a 1:1 ratio with SeraMag SpeedBeads in 19% w/v PEG 8,000 in a 1.5 ml LoBind tube ...
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bioRxiv - Cell Biology 2022Quote: For esiRNA transfection 35,000 U2OS cells were seeded in 2 ml medium in 6-well plates (Eppendorf) the evening before transfection ...
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bioRxiv - Immunology 2024Quote: ... 10 g NaCl) in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf), were transformed with a I53-50B.4PT1-encoding plasmid ...
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bioRxiv - Immunology 2024Quote: ... The mixture was incubated at 37°C for 2 h while shaken with a Thermomixer comfort (Eppendorf) at a setting of 300 rpm ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 30 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Immunology 2022Quote: ... 20 μL of mAb solution was added to the cells in the filter wells and the cells were incubated for 1 h at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). After the incubation ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 45 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was spun down at 13600 rpm for 10 minutes at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysate was spun down at 13600 rpm for 10 min at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Immunology 2021Quote: ... The 80 µL aliquot was lyophilised for 4 h at 30 °C with the aid of a vacuum concentrator (Eppendorf Concentrator 5301) attached to a refrigerated condensation trap (Savant) ...
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bioRxiv - Systems Biology 2021Quote: ... Total volume of 20 mL from each culture was transferred to a 50 mL Falcon® filled with ice and was immediately centrifuged at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). Next the supernatant was discarded and the cell pellet was snap frozen into liquid nitrogen and stored at -80 °C ...
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bioRxiv - Systems Biology 2021Quote: For the extraction of total proteome 10 mL of each culture were transferred into ice-cold 15 mL Falcon® tubes which were centrifuged immediately at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). The supernatant from the centrifugation was discarded and the cell pellets were washed once with 1 mL of cold PBS buffer ...
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bioRxiv - Biophysics 2020Quote: ... 200 ml of M9 minimal media were added to 4 mL of pre-inoculum and the cell growth was monitored using Biophotometer D30 (Eppendorf, Hamburg, Germany) until the growth rate was 0.8 (OD at 600 nm) ...
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bioRxiv - Microbiology 2022Quote: ... coli S17-1 that contained the shuttle- or suicide-vector construct at 3700 rpm for 10 min at room temperature (Centrifuge 5920 R, rotor S-4×1000, Eppendorf, Hamburg, Germany). We mixed the E ...
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bioRxiv - Cell Biology 2021Quote: Live-cell microinjection experiments were performed using a FemtoJet® 4i microinjector in conjunction with an InjectMan® 4 micromanipulation device (Eppendorf). All microinjections were performed using pre-pulled Femtotips® injection capillaries (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... freshly laid embryos were aligned against nitrocellulose paper and injected using a quartz capillary needle, three-axis micromanipulator (Narishige, MMO-4) and microinjector (Eppendorf FemtoJet 4X).
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bioRxiv - Zoology 2023Quote: ... The homogenates were centrifuged for 10 minutes (860 g at 4 °C) to remove the solid particulates (5810R, Eppendorf centrifuge, Hamburg, Germany) and the supernatant was removed and stored at -80 °C for later analysis [19] ...
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bioRxiv - Microbiology 2023Quote: Liquid samples in 1 ml were collected and centrifuged for 20 min at 21 130 × g at 4°C (Centrifuge 5424 R; Eppendorf, Hamburg, Germany). The supernatants were used for chemical analyses by applying external standards for calibration and quality control ...