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Citations for Eppendorf :
651 - 700 of 964 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: The filled needle is positioned on a micromanipulator (Narishige MMO-4) and connected to a positive pressure pump (Eppendorf FemtoJet 4i). Control embryos were injected with Cas9 protein and Lap2b-GFP mRNA only.
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bioRxiv - Biophysics 2023Quote: ... Lysate and cell fragments were separated by centrifugation at 4 °C for 20 min at 12,000 rpm (Centrifuge 5418 R, Eppendorf, Hamburg, Germany). Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The cell culture supernatant was collected and pre-cleared from cells and larger particles by centrifugation at 400 × g at 4 °C for 10 min (Eppendorf 5810R) and 10,000 × g at 4 °C for 30 min (Eppendorf 5910R) ...
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bioRxiv - Biochemistry 2023Quote: ... in 5% glucose solution v/v prior to adding 2X solubilization buffer and incubation at 37 °C for 4 hrs under gentle orbital agitation (500 rpm on Eppendorf Thermomixer). The product was then analyzed by SEC as described earlier.
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bioRxiv - Developmental Biology 2022Quote: ... The filled needle is positioned on a micromanipulator (Narishige MMO-4) and connected to a positive pressure pump (Eppendorf FemtoJet 4i). Embryos are placed in FHM drops covered with mineral oil under Leica TL Led microscope ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 1 hour at a temperature of 4°C with shaking at 600 rpm in a Thermomixer comfort (Eppendorf, Germany). Stained cells were washed with ice cold FACS buffer twice before resuspension in FACS buffer for analysis ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was used to fractionate the cell lysate (at 4 °C for 30 min at 4,416 g, Eppendorf, Centrifuge 5804 R) and at 4 °C for 1 hour for ultracentrifugation (70,658 g ...
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bioRxiv - Cell Biology 2024Quote: ... The protoplasts were collected by centrifugation at 1700 rpm for 1 minute using a swing-bucket rotor (Eppendorf S-4-72), washed with 15 mL of ice-cold solution 3 (26.4 g of ammonium sulfate ...
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bioRxiv - Cell Biology 2024Quote: ... the resuspended cilia were centrifuged at 16000 × g for 10 minutes at 4°C in a microfuge (Eppendorf, Centrifuge 5415 D), and the supernatant was removed ...
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bioRxiv - Microbiology 2024Quote: ... An overnight culture of 1 ml was pelleted down by centrifugation ((10,000 rpm at 4°C for 10 min; Eppendorf centrifuge 5810R) and washed thrice with M9 minimal (M9M ...
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bioRxiv - Physiology 2024Quote: ... plasma samples were thawed from storage at -80°C and centrifuged (5,000 x g, 10 minutes, 4 °C; Eppendorf 5427R with rotor FA-45-48-11; Eppendorf, Enfield, CT). Supernatants were transferred to 96-well plates and stored at -80°C until analysis ...
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bioRxiv - Neuroscience 2024Quote: ... They were then resuspended to a concentration of ∼800-3000 nuclei per uL across 3-4 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512) in NSB ...
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bioRxiv - Systems Biology 2021Quote: ... 5 ml of working volume was used in the batch culture at 37°C on a rotary shaker (Eppendorf). The pH of the growth medium was set to 7 ± 0.05.
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bioRxiv - Genomics 2022Quote: ... Multiple 20µm tissue curls were cut per tissue and collected in pre-cooled 5 ml tubes (Eppendorf, Hamburg, Germany) ensuring no thawing while transferring and stored on dry ice until processing ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). All used cell lines were confirmed to be mycoplasma free by using MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Genetics 2021Quote: ... A study pool quality control (SPQC) sample was prepared by combining 5 µL of supernatant from each sample into a 1.5 mL tube (Eppendorf). Stable Isotope Labeled (SIL ...
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bioRxiv - Cell Biology 2021Quote: ... spun at 1,000 x g at −9°C for 5 minutes then aliquoted into lo-bind 2ml centrifuge tubes (Eppendorf) and snap frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: Sheep or horse erythrocytes were washed three times with sterile PBS (pH=7.4) at 300⍰×⍰g for 5⍰min in a refrigerated centrifuge (Eppendorf). These washed erythrocytes suspension was lysed with 0.1% saponin and the concentration of oxy-hemoglobin in this preparation was determined using the QuantiChrom™ Hemoglobin Assay Kit (BioAssay Systems) ...
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bioRxiv - Microbiology 2022Quote: ... supernatants containing planktonic bacteria were removed from cultures and bacteria were pelleted down by centrifugation at 13,000 rpm x 5 min in a refrigerated micro-centrifuge (Eppendorf). In another set of experiments ...
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bioRxiv - Plant Biology 2023Quote: ... Bound phosphopeptides were then eluted 3 times with 100 µL ammonium hydroxide (5% v/v) into 1.5mL Lo-Bind tubes (Eppendorf). These were then frozen and lyophilized ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae strain to 5 mL of YPD media and incubating in an orbital shaker incubator (Innova 42, Eppendorf, UK) at 30 °C and 250 rpm overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and kept at 37°C with 5% CO2 in an incubator (New Brunswick Galaxy 170 S, Eppendorf, Schönenbuch, Switzerland).
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bioRxiv - Biophysics 2024Quote: ... to a final concentration of 200 nM PCt and 0.1-8 M GdmCl in 5 ml Protein LoBind tubes (Eppendorf). To avoid time-dependent photobleaching ...
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bioRxiv - Genomics 2021Quote: ... snap-frozen in five aliquots (Safe-Lock cup DNA LoBind 2 ml PCR clean tubes, Eppendorf, #0030108078) and stored at -80 °C ...
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bioRxiv - Genomics 2021Quote: ... the tube was incubated at room temperature for 2 minutes on a thermomixer (Eppendorf Thermomixer C, #5382000023) set to 1250 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... and lysed by pipetting for total RNA isolation or collected in 2 ml safe-lock tubes (Eppendorf) in 1x PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Snap-Cap Microcentrifuge Biopur™ Safe-Lock™ or Safe-Lock Tubes 2 mL were from Eppendorf™ ...
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bioRxiv - Microbiology 2020Quote: ... 10 g NaCl) in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf) were used to express the I53-50A or I53-50B.4PT1 proteins grown ...
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bioRxiv - Immunology 2022Quote: ... followed by pooling of the eluates and complete drying in 2 mL protein LoBind tubes (#0030108450, Eppendorf). For further purification ...
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bioRxiv - Immunology 2022Quote: ... 384 wells (the entire plate, including negative controls) were pooled in a single 2 ml tube (Eppendorf). Purification of cDNA was performed by adding 400 μl of cDNA in a 1:1 ratio with SeraMag SpeedBeads in 19% w/v PEG 8,000 in a 1.5 ml LoBind tube ...
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bioRxiv - Synthetic Biology 2024Quote: ... COVID_ssRNA & SARS-CoV-2 mimic) triggers were freshly prepared by serial dilution in DNA LoBind tubes (Eppendorf) using nuclease-free water ...
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bioRxiv - Genetics 2024Quote: ... Microinjection was performed using an Eppendorf TransferMan NK 2 and a FemtoJet 4i system (Eppendorf, Hamburg, Germany). The eggs used for microinjection were generated by mating female and male wild-type Dazao moths ...
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bioRxiv - Genomics 2024Quote: ... The sheared DNA was transferred to a 2 ml DNA LoBind® Tube (Eppendorf Cat. No. 022431048) and temporary stored at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... The resultant DNA was saved in a 2 mL DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C until library preparation ...
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bioRxiv - Genomics 2024Quote: ... The sheared DNA was collected with a 2 ml DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C until library preparation ...
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bioRxiv - Physiology 2023Quote: ... for EVs isolation on cell culture flask T-75 (1.5 − 2 × 106/ per flask; Eppendorf, Hamburg, Germany) and for immunostaining on Cell Imaging Cover Glasses (0.1 × 106 / per chamber ...
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bioRxiv - Cell Biology 2022Quote: For esiRNA transfection 35,000 U2OS cells were seeded in 2 ml medium in 6-well plates (Eppendorf) the evening before transfection ...
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bioRxiv - Immunology 2024Quote: ... 10 g NaCl) in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf), were transformed with a I53-50B.4PT1-encoding plasmid ...
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bioRxiv - Immunology 2024Quote: ... The mixture was incubated at 37°C for 2 h while shaken with a Thermomixer comfort (Eppendorf) at a setting of 300 rpm ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 30 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Immunology 2022Quote: ... 20 μL of mAb solution was added to the cells in the filter wells and the cells were incubated for 1 h at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). After the incubation ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 45 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was spun down at 13600 rpm for 10 minutes at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysate was spun down at 13600 rpm for 10 min at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Immunology 2021Quote: ... The 80 µL aliquot was lyophilised for 4 h at 30 °C with the aid of a vacuum concentrator (Eppendorf Concentrator 5301) attached to a refrigerated condensation trap (Savant) ...
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bioRxiv - Systems Biology 2021Quote: ... Total volume of 20 mL from each culture was transferred to a 50 mL Falcon® filled with ice and was immediately centrifuged at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). Next the supernatant was discarded and the cell pellet was snap frozen into liquid nitrogen and stored at -80 °C ...
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bioRxiv - Systems Biology 2021Quote: For the extraction of total proteome 10 mL of each culture were transferred into ice-cold 15 mL Falcon® tubes which were centrifuged immediately at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). The supernatant from the centrifugation was discarded and the cell pellets were washed once with 1 mL of cold PBS buffer ...
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bioRxiv - Biophysics 2020Quote: ... 200 ml of M9 minimal media were added to 4 mL of pre-inoculum and the cell growth was monitored using Biophotometer D30 (Eppendorf, Hamburg, Germany) until the growth rate was 0.8 (OD at 600 nm) ...
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bioRxiv - Microbiology 2022Quote: ... coli S17-1 that contained the shuttle- or suicide-vector construct at 3700 rpm for 10 min at room temperature (Centrifuge 5920 R, rotor S-4×1000, Eppendorf, Hamburg, Germany). We mixed the E ...
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bioRxiv - Cell Biology 2021Quote: Live-cell microinjection experiments were performed using a FemtoJet® 4i microinjector in conjunction with an InjectMan® 4 micromanipulation device (Eppendorf). All microinjections were performed using pre-pulled Femtotips® injection capillaries (Eppendorf) ...