Labshake search
Citations for Eppendorf :
651 - 688 of 688 citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... in PBS in a 15mL tube and spun at 1000g for 10 min at room temperature with acceleration and brake set to 1 (Eppendorf 5810R). The supernatant was discarded ...
-
bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
-
bioRxiv - Biochemistry 2020Quote: ... Peptides were eluted with 60 μl of 80% acetonitrile/1% ammonia and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were dissolved and sonicated in 5% acetonitrile/0.1% TFA ...
-
bioRxiv - Microbiology 2021Quote: ... Bacillus cereus group isolates’ inocula were prepared from overnight cultures (see “Bacterial cultures” section) by adjusting their concentration to 1-2 × 108 CFU/ml using a spectrophotometer (Eppendorf BioPhotometer 6131). A previously established OD-CFU standard curve was used to estimate the CFU/ml based on the OD reading ...
-
bioRxiv - Systems Biology 2022Quote: ... Peptides were eluted with 60 μl of elution buffer (80% acetonitrile/1% ammonia) and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were redissolved and sonicated in 5% acetonitrile/0.1% TFA ...
-
bioRxiv - Biophysics 2022Quote: ... The cultures were then induced with IPTG by adding 10 uL of 100 mM (final concentration approximately 1 mM) per well with an electric repeater pipette (Eppendorf, E4x series), and grown for another 4 h at 37 °C ...
-
bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/ml of hygromycin and correct genome editing was confirmed by Sanger sequencing (Macrogen Europe ...
-
bioRxiv - Developmental Biology 2020Quote: ... Microinjection of young adult hermaphrodite germlines was done using an inverted microinjection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). In cases where the sgRNA target site was not disrupted by sequence integration ...
-
bioRxiv - Microbiology 2022Quote: ... 4.6×106 LMBs or NMBs were added and samples incubated at 37 °C for 1 hour on a thermomixer (60 rpm; Eppendorf, Hamburg, Germany). After this step ...
-
bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
-
bioRxiv - Biochemistry 2022Quote: ... samples were prepared in 96 well microtiter plates by adding 12.5 μL reaction supernatant to 50 μL acetonitrile with 1% v/v trifluoroacetic acid (TFA) followed by centrifugation (2,204 g, 30 min; A-2-DWP rotor, Eppendorf AG, Hamburg, Germany) and transferring of 50 μL centrifugation supernatant into 150 μL MilliQ H2O ...
-
bioRxiv - Neuroscience 2023Quote: ... The plasmid injection solution was injected into Hydra vulgaris AEP 1-cell stage embryos using an Eppendorf FemtoJet 4x and Eppendorf InjectMan NI 2 microinjector (Eppendorf; Hamburg, Germany) under a Leica M165 C stereo microscope (Leica Microscopes ...
-
bioRxiv - Systems Biology 2024Quote: ... are then added to the sample and incubated at 56°C for 1 hour at 1000 RPM (ThermoMixer C, Eppendorf, Hamburg, Germany). 37µl of 500mM chloroacetamide (50mM final concentration ...
-
bioRxiv - Plant Biology 2024Quote: ... SDS at a 1:3 (w/v) ratio and extracted by shaking 1000 RPM 95 °C for using a tabletop shaker (Eppendorf ThermoMixer F2.0). Samples were then centrifuged at 20,000 xg for 10 min at room temperature and the supernatant retained in new tubes ...
-
bioRxiv - Genetics 2024Quote: ... placed in an injection arena (9 cm-diameter Petri dish containing a layer of 1% agarose) and injected with ∼60 ng of Pmtra-2 or EGFP dsRNA (negative control) using a FemtoJet microinjector (Eppendorf, Hamburg, Germany). Injectees were maintained in a two-ounce plastic cup with corn leaves until they became adults ...
-
bioRxiv - Microbiology 2022Quote: ... Bacterial mixtures were diluted 1:1000 into LB with or without 1% DMSO (v/v) and incubated standing at 37°C in 5% CO2 at atmospheric oxygen (normoxic; Eppendorf CellXpert incubator), 1% oxygen (hypoxic ...
-
bioRxiv - Neuroscience 2023Quote: ... A total of 1800 µl of solution from both tubes (batch 1) were transferred to a 2 ml Eppendorf and vortexed (Eppendorf Thermomixer C) for 15 minutes at 2000 rpm (4 °C) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 100 ng corresponding pEcgRNA-N20 and 400 ng donor DNA were co-electrotransformed into 100 uL competent cells in 1 mm gap electroporation cuvette by applying 2.5 kV pulse in electroporator (Eppendorf™ Eporator™). After adding 900 uL of cold SOC medium ...
-
bioRxiv - Biochemistry 2024Quote: ... A second gDNA purification was coupled with reverse transcription of RNA (1 μg) into cDNA with the SuperScript IV VILO ezDNase system (Thermo Fischer Scientific) using an Eppendorf Mastercycler (Eppendorf, Hamburg, Germany). cDNA samples were entered into a custom TaqMan low density array (Thermo Fischer Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... castellanii trophozoites were seeded at the log growth phase (1 × 104 cells per well) in a 96-well white microplate (Eppendorf, Hamburg, Germany). Incubation with medium containing varied nitroxoline concentrations ranging from 0 to 100 μM was performed ...
-
bioRxiv - Neuroscience 2024Quote: ... Lyophilized peptide was dissolved in 100% HFIP solvent to a final concentration of 1 mM and centrifuged in a speed-vac (Eppendorf, Hamburg, Germany). Peptide pellet was then resuspended in DMSO to a final concentration of 5 mM followed by sonication at 37°C for 10 minutes ...
-
bioRxiv - Zoology 2024Quote: ... and sgRNA (150 ng/μL) was co-injected into embryos (<1 hr-old) using the InjectMan NI 2 microinjection system (Eppendorf, Hamburg, Germany). Injected eggs were incubated at 26 ± 1 °C and 70% ± 5% relative humidity for hatching.
-
bioRxiv - Biochemistry 2021Quote: ... Production of CjLPMO10AFL was achieved by fed-batch fermentation of the expression strain in a 1-liter fermenter (DASGIP® benchtop bioreactors for cell culture; Eppendorf, Hamburg, Germany), essentially as described previously (44) ...
-
bioRxiv - Microbiology 2020Quote: ... printed arrays were moved to individual 1 mL centrifuge tubes containing 200 μL of M9 medium and melted at 55°C for 1 min in a thermomixer (Eppendorf ThermoMixer® C) shaking at 1000 rpm ...
-
bioRxiv - Microbiology 2022Quote: ... Two-microliter aliquots of resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
-
bioRxiv - Microbiology 2022Quote: ... Two μl aliquots of the resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
-
bioRxiv - Bioengineering 2024Quote: ... 40 µL of clarified lysate was aliquoted into full-skirted PCR plates and incubated at the reported temperature for 1 h using a gradient on a Mastercycler® X50s (Eppendorf, Catalog # 6311000010). Room temperature controls were incubated for 1 h on the benchtop in 200-µL PCR tubes ...
-
bioRxiv - Genomics 2020Quote: The ST slide with the tissue section was warmed to 37°C for 1 minute on a thermal incubator (Eppendorf Thermomixer Option C, Germany). The tissue was then covered with 4% formaldehyde (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: ... The tissue was rinsed with fresh HBSS and digested with 20 U/ml papain (#LS003126, Worthington Biochemical) (500 μl per one hippocampi Eppendorf, 1 ml for cortex) for 30 minutes on thermomixer at 37°C with gentle shaking (350 rpm) ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein pellets were dissolved in 0.1% RapiGest and 500 mmol/L TEAB and then incubated at 850 rpm for 45 min at 37 °C (Eppendorf Comfort Thermomixer, Eppendorf, USA). Proteins were digested by trypsin (Trypsin Gold ...
-
bioRxiv - Bioengineering 2023Quote: ... Fluorescence measurements were taken at day 1 (when the PCL-TMA scaffolds were removed from the Eppendorf tube after 24 hours of cell seeding) and on day 14 (each PCL-TMA scaffold was moved to a new 24 well plate to ensure only the cells adhered to the scaffold were quantified) ...
-
bioRxiv - Cell Biology 2024Quote: ... SCs were proliferated in SFGM on Cytodex- 1 microcarriers (Cytiva) in a 50c BioBLU single use vessel (Eppendorf; 37 °C, pH 7.2, 25 rpm) with a working volume of 40 L ...
-
bioRxiv - Biochemistry 2024Quote: ... the samples received 50 µl 1 M NaOH (500 mM final concentration; dispensed with an insulin syringe by punching the lid of the Eppendorf, without opening the tube).
-
bioRxiv - Molecular Biology 2024Quote: ... 1:1v/v mixture of 33% ammonium hydroxide and 40% methylamine in water for 3 h at 37 °C (Eppendorf ThermoMixer C, 1000 rpm). The suspension was filtered ...
-
bioRxiv - Developmental Biology 2024Quote: ... The cells were washed with cold staining buffer (1× PBS, 0.2% BSA and 5 mM glucose) and spun down at 150 × g with low brake (Eppendorf Centrifuge 5810 R, Hamburg, Germany). The cell number was counted using a hemocytometer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Flowrate was adjusted at 2 ml/min to collect peptides in 1 ml fractions in low-protein binding Eppendorf tubes (Eppendorf LoBind tubes, cat. no. EPPE0030108.116). The bound complexes were separated from β2 microglobulin and heavy chain using increasing concentration of buffer B ...
-
bioRxiv - Bioengineering 2023Quote: ... Fluorescence measurements were taken at day 1 (when the PCL-TMA/PCL-TMA900 scaffolds were removed from the Eppendorf tube after 24 hours of cell seeding) and on day 14 (each scaffold was moved to a new 24 well plate to ensure only adherent cells were quantified) ...