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Citations for Eppendorf :
601 - 650 of 904 citations for 2 Bromo 1 5 bromofuran 2 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... We put 500 mL of log phase density (1×106-1×107 cells/mL) cells into 1 mL disposable cuvettes (UVette, 952010051, Eppendorf AG, Hamburg, Germany). We measured the OD600 every hour for 7 hours in each of two cuvettes for each cell line ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × Denhardt’s solution (Eppendorf, 0032007.155). Hybridization was performed with a double DIG-labeled locked nucleic acid (LNA ...
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bioRxiv - Developmental Biology 2021Quote: ... the heads were cleaned with alcohol and a 1μl single cell suspension (5 x 106 cells/ml) was manually injected directly into the left cerebellar hemisphere using a glass microcapillary (Eppendorf, 5195 000.079) controlled with an Eppendorf CellTram Vario manual microinjector ...
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bioRxiv - Microbiology 2020Quote: ... The overnight culture was diluted in Mueller Hinton Broth (MHB) based on the OD600 to obtain 5 × 105 CFU/mL using a BioPhotometer D30 (Eppendorf, Hauppauge, NY). An OD600 of 1 was assumed to be equal to 8 × 108 CFU/mL.
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bioRxiv - Cancer Biology 2020Quote: ... sonicated for 15 min and vigorously mixed at 5°C for 30 min with 2000 rpm on a ThermoMixer (Eppendorf, Hamburg, Germany). Samples were centrifuged for 5 min at 18000 rpm ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We separated the tissue from the buffer and reproductive fluid by centrifuging at 4ºC for 5 min at 3500 rpm (Eppendorf Centrifuge 5702RH, USA), pipetted the supernatant from each tract region into microplate wells (CellStar 96 Well Cell Culture Plate ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were pelleted by centrifugation at approximately 250 × g for 5 min at 4 °C (Eppendorf 5804 R, Hamburg, Germany). After resuspension ...
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bioRxiv - Microbiology 2020Quote: ... we centrifuged a 0.5-mL sample of culture in the exponential phase at 7000 rpm for 5 min in a Benchtop centrifuge (5424 Eppendorf, Hamburg, Germany). We washed the pelleted biomass 1 – 2 times and resuspended it with 50 µL 1x PBS from which we fixed 2 µL on solidified agarose (VWR ...
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bioRxiv - Genomics 2021Quote: ... then harvested with a sterile cell scraper while at -20°C and transferred to −20 °C cold 5 mL centrifuge tubes (Eppendorf Lo-Bind). After centrifuging the cell-extracts in a 4 °C centrifuge for 5 min at 2000 x g ...
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bioRxiv - Cancer Biology 2023Quote: For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were maintained between 10% and 80% confluence and kept at 37 °C with 5% CO2 (8% CO2 for VPC cells) in a humidified CO2 incubator (Eppendorf, Enfield, CT). The viable cell density (VCD ...
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bioRxiv - Microbiology 2024Quote: ... one with the blood mixed to RPMI and the second with the gametocyte suspension were spun down at 2000rpm for 5 minutes in a temperature-controlled centrifuge at 37°C (Eppendorf 5702 R). RPMI supernatant was removed from both tubes with 3ml disposable Pasteur pipettes ...
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bioRxiv - Microbiology 2023Quote: ... CNW Technologies) using 80% ACN/5% NH4OH and dried at 45°C using a vacuum centrifuge (cat. no. 5305, Eppendorf, Hamburg, Germany). The peptide samples were resolved in 2% ACN/0.1FA for LC-MS analysis ...
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bioRxiv - Biochemistry 2022Quote: ... The resin was pelleted down by centrifugation at 4°C (1258 g for 5 min, A-4-81 rotor, Eppendorf, Enfield, CT) and washed with ice-cold 50 mL Binding buffer composed of 10mM imidazole (pH 7.4) ...
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bioRxiv - Genomics 2023Quote: ... The bone pieces in 5 ml tube of DNA Lobind were treated pre-digestion for 15 minutes at 900 rpm in a Thermomixer (Eppendorf, Hamburg, Germany) with the lysis buffer (2 ml ...
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bioRxiv - Developmental Biology 2023Quote: ... the gRNA:Cas9 ribonucleoprotein complex solution was incubated at 37°C for 5 min and then backfilled into 3 microinjection needles using an Eppendorf GELoader tip (Eppendorf, Cat# 022351656). After loading embryos into the embryo holder which covered with 12.5 ppt of salinity water with 0.0001% of methylene blue ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was collected after centrifugation at 4 °C for 5 min at 5000 g and dried using a SpeedVaq (Eppendorf, Montesson, France). Then ...
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bioRxiv - Microbiology 2024Quote: ... The peptides were eluted in glass vials (CNW Technologies, cat#A3511040) using 80% ACN/5% NH4OH and dried at 45 °C using a vacuum centrifuge (Eppendorf, cat#5305). The peptide samples were resolved in 2% ACN/0.1FA for LC-MS analysis ...
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bioRxiv - Immunology 2024Quote: ... stool suspensions of feces (in H2O) were centrifuged at 16000 rpm and 4°C for 5 min using an Eppendorf 5427R centrifuge (Eppendorf; Hamburg, Germany). 100 µL of supernatant were mixed with 400 µL of ice-cold methanol containing recovery standards for evaluation of the quality of cell harvest and correction for variations ...
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bioRxiv - Immunology 2024Quote: ... Before preparation for LC-MS analysis each sample was centrifuged at 16000 rpm and 4°C for 5 min using an Eppendorf 5427R centrifuge (Eppendorf; Hamburg, Germany). From each supernatant 350 µL were pipetted into two separate LC vials ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged for 5 min at 16,000× g and the resulting supernatants were transferred to new microcentrifuge tubes before incubation at 99 °C for 5 min in a thermomixer (Eppendorf, Hamburg, Germany).
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bioRxiv - Microbiology 2024Quote: ... 1 ml of culture was centrifuged at 4000 x g for 5 min in a 1.5 ml reaction tube (Eppendorf AG, Hamburg, GER), and the resulting cell pellet was washed twice with 500 μl 1x PBS containing 1 g/L L(+)-ascorbic acid (AppliChem GmbH ...
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bioRxiv - Systems Biology 2024Quote: ... was recorded and 20 ml of culture were centrifuged at 1,500 x g for 5 minutes at RT (Eppendorf Centrifuge 5810 R). After centrifugation ...
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bioRxiv - Plant Biology 2024Quote: ... homogenized by vortexing and incubated at room temperature with Protein Assay Dye Reagent for 5 minutes before absorbance reading at 595 nm (Eppendorf Biophotometer D30).
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bioRxiv - Microbiology 2020Quote: ... 20 mL of the enrichment was transferred to 5 L NMS medium in a 6-L fermenter controlled with a BioFlo® 120 Bioprocess Control Station (Eppendorf, Hamburg, Germany). Gas stream carrying a relatively low CH4 concentration (0.5% v/v in air ...
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bioRxiv - Microbiology 2024Quote: ... and 40 mL of the supernatant was added as the inoculum to 5 L medium prepared in a BioFlo® 120 bioreactor (Eppendorf, Hamburg, Germany). Air containing 0.5% or 10% (v/v ...
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bioRxiv - Pathology 2024Quote: ... Each plate was heated to 37°C for 60 min and 95°C for 5 min in a thermocycler (Eppendorf Mastercycler, Nexus SX5). Each well was then diluted ten times so as to obtain an estimated cDNA concentration of 50 ng/µL.
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bioRxiv - Cancer Biology 2024Quote: ... Aliquots of cells in αMEM were transferred to centrifuge tubes and centrifuged for 5 minutes at 900 rpm (Eppendorf 5804 R Benchtop Centrifuge). After removing the αMEM media ...
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bioRxiv - Microbiology 2022Quote: ... 1% oxygen (hypoxic; Eppendorf CellXpert incubator), or 0% oxygen (anoxic ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The sequencing failed for 1 sample and 1 sample was damaged during the travel (Eppendorf tube burst), decreasing our total sample size to 41 ...
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bioRxiv - Plant Biology 2024Quote: ... Duplicate samples of 1 mL were incubated for 1 h at 50 °C in a thermomixer (Eppendorf) with shaking at 600 rpm with 5 µL of CTec3 diluted 10-fold from the commercial product added to each sample ...
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bioRxiv - Genetics 2021Quote: ... 72°C 45 s and a final extension at 72°C for 5 min was run on an Eppendorf Mastercycler® (Eppendorf AG, Hamburg Germany). Amplicons were observed on a 1.5% agarose gel (Bioline (Aust ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a mixture of the antibody constructs with a 10-fold molar excess of DFO*-NCS (5 mg/mL in DMSO) (ABX, Radeberg, Germany) was incubated ON at 4 °C with gentle shaking (Eppendorf ThermoMixer, Merck, Darmstadt, Germany). The DFO*-derivatized constructs were purified by SE-HPLC under the same conditions as described above.
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bioRxiv - Neuroscience 2024Quote: ... The adjacent 3-5 sections of 100 micron tissue sections were collected in a pre-chilled 2mL microcentrifuge tube (Eppendorf Protein LoBind Tube, Cat #22431102) to total a volume of approximately 40 mg for each donor ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein concentration determined by nanodrop (assuming an extinction coefficient of εMolar = 46410 M−1cm−1. The protein solutions were moved into 1 mL aliquots in 1.5 mL Eppendorf tubes ...
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bioRxiv - Microbiology 2022Quote: ... trypsin was inactivated with 1:1 complete media and the cell suspension was collected into a 5mL protein-low bind centrifuge tube (Eppendorf). To remove residual trypsin ...
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bioRxiv - Cancer Biology 2022Quote: ... Pups from these females were injected at P2 with the two plasmids mixed at a ratio of 1:1 using a Femtojet microinjector (Eppendorf) directly into the lateral ventricle ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA concentration was measured in a 1 μl sample diluted 1:200 in nuclease-free water in a spectrophotometer (BioPhotometer, Eppendorf). cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... 1 min), annealing (65°C, 1 min), and elongation (72°C, 1 min) were performed in a Mastercycler thermal cycler (Eppendorf, Germany). A final extension step was added for 10 min at 72 °C at the end of the 30th amplification cycle ...
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bioRxiv - Neuroscience 2024Quote: ... The forebrain was dissected and weighed before lyophilization at a shelf temperature of –56 °C for 24h under vacuum of 0.2 mBar (Christ LMC-1 BETA 1-16) and extraction with formamide at 57°C for 24h on a shaker at 300 rpm (Eppendorf Thermomixer). Integrated density of tracer fluorescence was determined in triplicates after 1:3 ethanol dilutions ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 times for 5 seconds each to ensure homogenization and centrifuged at 14000 x g for 10 minutes at 4° C (Eppendorf centrifuge 5424 R, FA-45-24-11 rotor). Supernatant was precleared by rotating 20 µl Protein A agarose beads (Cell Signaling #9863 ...
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bioRxiv - Developmental Biology 2021Quote: ... A Femtotip microcapillary (1 μm tip diameter, Eppendorf, Germany) was mounted onto a Femtojet pump and micromanipulator (Eppendorf) ...
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bioRxiv - Bioengineering 2023Quote: ... MPCs were washed twice for 3 min at 400 RCF at room temperature with PBS supplemented with 1% glucose and 1% pen-strep (Eppendorf 5702R, Hamburg, Germany). Biotinylation of the cell surface was performed by adding 1 mM Sulfo-NHS-LC-Biotin (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... and agitated (1,300rpm, 30 min, 1°C; Thermomixer Comfort, Eppendorf). Solubilized membrane proteins were obtained in the supernatant from this agitated suspension by centrifugation (21,000g ...
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bioRxiv - Immunology 2020Quote: ... The pH of the eluate (1 mL per Eppendorf tube) was neutralized (125 µL of Tris 1M ...
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bioRxiv - Microbiology 2020Quote: ... The remaining 1 mL of culture was centrifuged (Eppendorf 5417R) at 8000xg and washed twice with 1 mL of LB media ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 ml aliquots were concentrated in a speed-vac (Eppendorf) for 45 min at 30°C ...
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bioRxiv - Physiology 2023Quote: ... for 1 h at 4 □ on a ThermoMixer C (Eppendorf), 350 µl of water and 250 µl of chloroform were added to the mixture to induce phase separation ...
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bioRxiv - Neuroscience 2024Quote: ... regionalized neural organoids (1-3 organoids per one Eppendorf tube) were transferred into a 1.5 mL Eppendorf tube containing 200 μL of the neural medium ...
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bioRxiv - Bioengineering 2024Quote: ... centrifuged (Eppendorf 5810R, 3,220 x g, 4 °C, 1 min), and the absorbance was measured at 494 nm (CLARIOstar ...