Labshake search
Citations for Eppendorf :
551 - 600 of 701 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... incubated with each antigen at certain concentration in PBS/2% FCS and transfer to a 37°C thermomixer (Eppendorf). Cells were collected after each period of incubation and fixed in IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
-
bioRxiv - Evolutionary Biology 2020Quote: GV oocytes were microinjected with ~5 pl of cRNAs in M2 medium (with 2.5 mM milrinone and 3mg/mL BSA) at room temperature (RT) with a micromanipulator TransferMan NK 2 (Eppendorf) and picoinjector (Medical Systems Corp.) ...
-
bioRxiv - Cell Biology 2020Quote: ... The homogenate was further incubated on ice for 45 min before centrifugation at 21,000 g and 4°C for 2 x 10 min in an Eppendorf 5424 R benchtop centrifuge (Eppendorf). Protein content of the lysate was determined by BCA assay (Thermo Fisher) ...
-
bioRxiv - Immunology 2020Quote: ... 293T-hACE2-FFLuc-GFP-RBD cells were transfected with 0.25 μg of SARS-CoV-2-RBD plasmid and 0.25 μg of pHAGE-FFLuc-GFP each well in a 24-well plate (Eppendorf) for 48 hours at 37°C under 5% (v/v ...
-
bioRxiv - Molecular Biology 2022Quote: ... we further purified the extracts by treatment with 1% sodium dodecyl sulfate in NaP-EDTA buffer and centrifugation at 13,000 rpm for 10 min (rotor FA-24×2, Eppendorf). This purification process was repeated two times ...
-
bioRxiv - Genomics 2024Quote: ... The resultant DNA was collected and stored in a 2 mL DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C until library preparation ...
-
Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... cleared by centrifugation and transferred to a clean 2 ml 96-well deep-well plate (DWP, Eppendorf, Hamburg, Germany). TiO2 beads (Titansphere® Phos-TiO Bulk 10 µm ...
-
bioRxiv - Cell Biology 2023Quote: ... Oocytes were then microinjected with ~5 pl of mRNAs in M2 medium with 2.5 mM milrinone and 3mg/mL BSA at room temperature with a micromanipulator TransferMan NK 2 (Eppendorf) and picoinjector (Medical Systems Corp.) ...
-
bioRxiv - Genomics 2022Quote: ... Supernatant was transferred using a wide-bore pipette to a 2 mL DNA low-bind tube (Eppendorf, Enfield CT), precipitated with 1 mL of 100% ethanol ...
-
bioRxiv - Neuroscience 2023Quote: ... and tubes centrifuged at 4 °C for 2 min at 12000 x g in a refrigerated microfuge (Eppendorf 5415R). The upper phase was discarded ...
-
bioRxiv - Cell Biology 2023Quote: ... The sample was incubated at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany) to reduce viscosity ...
-
bioRxiv - Molecular Biology 2024Quote: ... peptides were eluted in 40 μl of 80% acetonitrile in 0.1% TFA and concentrated down to 2 μl by vacuum centrifugation (Concentrator 5301, Eppendorf). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μl by 0.1% TFA ...
-
bioRxiv - Immunology 2024Quote: ... a single mouse kidney was weighed and placed in a 2 mL safelock microcentrifuge tube (#05-402-12, Eppendorf) containing 1 mL kidney extraction buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... and flash frozen in liquid nitrogen as a pellet in a 2 mL Eppendorf Safe-Lock tube (Eppendorf #0030123620) with a 7 mm steel ball (Retsch #05.368.0035) ...
-
bioRxiv - Genomics 2024Quote: ... The supernatant was removed and cells were resuspended in 2×100µl F-buffer in a 1.5mL low binding tube (Eppendorf, 022431021). Cells were counted and Freezing Buffer (F ...
-
bioRxiv - Biophysics 2021Quote: ... Experiments on the seconds to minutes time scale were performed at room temperature in 96-well Protein LoBind plates (Eppendorf, Hamburg, Germany).
-
bioRxiv - Genomics 2020Quote: ... 3X in Ribohyb buffer and resuspended in a final volume of 10 ul Ribohyb buffer in a non-stick tube (Eppendorf Protein LoBind). The 10 µl hybridization reactions were added to 10 µl of resuspended beads for a binding reaction volume of 20 µl ...
-
bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
-
bioRxiv - Neuroscience 2020Quote: Aggregate enrichment was performed by centrifugation of a total of 150 μg protein at 20’800 g in a tabletop centrifuge (Eppendorf 5417r, Hamburg, Germany) for 1 hour at 4°C ...
-
bioRxiv - Biophysics 2021Quote: ... The protein samples of N-NTD and N-NTD-SR at 20 µM were prepared in low-binding microtubes (Eppendorf® LoBind) containing 20 mM Tris-HCl buffer (pH 7.5 ...
-
bioRxiv - Systems Biology 2022Quote: ... each SCN was cut into 3 pieces with a scalpel on a glass slide and transferred to a protein LoBind tube (Eppendorf, Hamburg, Germany) prefilled with 250 µL lysis buffer (100 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2022Quote: ... The crude extract was centrifuged for 15 min at 13,000 rpm at 4 °C to remove any tissue debris and the clear supernatant was transferred to a fresh Protein Low-Bind tube (Eppendorf, Hamburg, Germany). The total protein content of the samples was estimated using Pierce BCA protein assay kit from Thermo Scientific (Rockford ...
-
bioRxiv - Genomics 2023Quote: ... approximately 1.0-2.0 x 105 GFP+ neurons per pup were collected by FACS into a 1.5 mL Protein LoBind Tube (Eppendorf, cat. no. #Z666505).
-
bioRxiv - Biochemistry 2023Quote: ... Samples were thawed and Urea was added to a final concentration of 8 M urea and the protein lysate was transferred to 2mL snap-cap centrifuge tubes (Eppendorf, Hamburg, Germany) with 0.1 mm zirconia beads and bead beat in a Bullet Blender (Next Advance ...
-
bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
-
bioRxiv - Neuroscience 2024Quote: ... The top phase was carefully removed and then the bottom phase was carefully transferred to a new tube without removing protein from the intermediate layer and dried without heat in a vacuum centrifuge (Vacufuge Plus, Eppendorf, Enfield, CT) and stored at -80°C until MS analysis.
-
bioRxiv - Plant Biology 2024Quote: ... homogenized by vortexing and incubated at room temperature with Protein Assay Dye Reagent for 5 minutes before absorbance reading at 595 nm (Eppendorf Biophotometer D30).
-
bioRxiv - Biochemistry 2024Quote: ... Digestion was stopped using 1% trifluoroacetic acid (TFA) and the resulting peptides were collected using a magnetic rack and transferred into protein Lo-Bind Eppendorf tubes (Eppendorf, Hamburg, Germany). The peptides were stored at - 80°C and were dried using a vacuum concentrator (CentriVap® Concentrator ...
-
bioRxiv - Cell Biology 2020Quote: ... The whole lane was excised from the gel and future cut into approximate 3 x 1 x 2 mm3 slices (L x W x H) and each slice were placed into a separate LoBind tubes (Eppendorf) for destaining ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA was eluted with 2 x 100 μl of fresh elution buffer (100 mM dithiothreitol in RNase-free H20) directly into 2 ml lobind tubes (Eppendorf) containing 700μl Buffer RLT (RNeasy MinElute Cleanup Kit ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Soaked biofilms were gently removed from the agar surface using a sterile Drigalski spatula and a pipette tip and together with the unabsorbed stabilization mix transferred into a 2 mL tube (Eppendorf). An additional 1 mL of stabilization buffer was added to the tubes and the content was homogenized with a sterile pestle ...
-
bioRxiv - Developmental Biology 2021Quote: ... the tissue samples were weighed (between 1.4 - 11.0 mg) and transferred into a 2 mL screw cap Eppendorf plastic tubes (Eppendorf, Stevenage, UK) along with a single 5 mm stainless steel ball bearing ...
-
bioRxiv - Neuroscience 2022Quote: ... The homogenate was filtered through a 50 m.um filter (Sysmex; 04-004-2327) into a 2 mL microcentrifuge tube (Eppendorf; 022431048). An additional 0.5 mL of homogenization buffer was used to wash the Dounce homogenizer and filter ...
-
bioRxiv - Biophysics 2020Quote: ... The needle was then glued to a 6×4×2 mm Neodyn magnet (QM-06×04×02-N, Magnets4you) and attached to a motorized micromanipulator (PatchMan, Eppendorf). The magnetic needle was lowered until it touched the bottom of a dummy sample dish and raised again so that it was placed above the bottom of the sample with the very tip in focus around 100 μm above the focus of the glass surface.
-
bioRxiv - Microbiology 2021Quote: ... The surrounding SCFM was directly transferred to individual sterile 2 ml DNA lo-bind tubes from the 24-well plate (Eppendorf).
-
bioRxiv - Biochemistry 2020Quote: ... Reactions were started by addition of 59.0 μM ubiquitin and 2 mM ATP then incubated at 37°C in a Mastercycler nexus (Eppendorf, Germany) thermocycler for 2 hrs unless otherwise stated ...
-
bioRxiv - Biochemistry 2020Quote: ... we gently shook the flower and collected pollen into a weighed 2 mL microcentrifuge tube (Eppendorf®, Safe-Lock™). For species for which pollen was more difficult to harvest ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were kept on ice and centrifuged at 14 krpm for 2 min on a mini spin plus centrifuge (Eppendorf) before measurement ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 μM purified TDP-43-MBP-His6 variants (WT, 5D, 12D, 12A) were set up in low binding tubes (Eppendorf) in 35 μl aggregation buffer (50 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C ...
-
bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 15-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C until further analysis ...
-
bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and sonicated in chilled PBS (1 s on ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was transferred into a new 2 mL Eppendorf tube and evaporated to dryness using a Concentrator Plus (Eppendorf) (V-AL ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were spun at 10,000 g for 15 min (spin 2 in the same Eppendorf FA-45-48-11 rotor) and the resulting supernatant is the tissue lysate (TL ...
-
bioRxiv - Immunology 2022Quote: ... For that 2.5x105 cells were incubated with HIV-1 antigens in PBS/2% FCS for the indicated period of times on a 37°C thermomixer (Eppendorf). Cells were then fixed with IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
-
bioRxiv - Microbiology 2022Quote: ... Isolated midguts or the mosquito carcass (remainder of the body without midgut) were placed separately into 2 ml safe-lock Eppendorf tubes (Eppendorf) containing 250 µl of mosquito diluent (1 × PBS supplemented with 20% FBS ...
-
bioRxiv - Biochemistry 2022Quote: ... Annealing was carried out by heating the mixture to 95 °C for 2 minutes followed by gradual cooling to 25 °C over 45 minutes in a thermocycler (Eppendorf).
-
bioRxiv - Developmental Biology 2020Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using FemtoJet or FemtoJet 4i (Eppendorf; 5252000013) micro-injectors ...
-
bioRxiv - Neuroscience 2020Quote: ... or vehicle for thirteen days from postnatal day 2 (P2) to postnatal day 14 (P14) using a micropipette (0.5-10 µL; Cat. No. 3123000020; Eppendorf, Germany). Mouse pups for each postnatal treatment experiment were selected from at least six different dams to minimize any litter-related bias ...
-
bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in 1mL of ice-cold PBS and transferred to 2 mL LoBind Eppendorf tubes (Eppendorf #022431102). Cells were centrifuged for 2 minutes at 800rpm before being flash-frozen on dry ice ...