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Citations for Eppendorf :
501 - 550 of 1303 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the plate was returned to the magnet and for 2 minutes and 48 μl of purified DNA was transferred to a fresh 96-well PCR plate (Eppendorf 0030 128.648).
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bioRxiv - Neuroscience 2021Quote: ... a whole eye from wild type and mutant adult zebrafish or 100 zebrafish mutant larvae were transferred to 2 ml Eppendorf microcentrifuge tubes (Eppendorf, Hamburg, Germany). 200 μl of 2 M hydroxylamine (pH 8 ...
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bioRxiv - Immunology 2022Quote: ... Purified IgG was digested into F(ab’)2 with 200 μg of IdeS per 10 mg of IgG for 6 hr on a thermal mixer (Eppendorf ThermoMixer C) at 37°C ...
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bioRxiv - Genomics 2024Quote: ... and Cas9 protein (100 ng/μl) were injected into individual eggs using a FemtoJet and InjectMan NI 2 microinjection system (Eppendorf, Hamburg, Germany). The microinjection process was completed within 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... four times (1600 μL) ice-cold 80% acetone was added to 400 μL of urine in 2 ml protein Lo-Bind tubes (Eppendorf, Hamburg, Germany). After incubating at −20 °C for 1hr ...
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bioRxiv - Genetics 2024Quote: ... and Cas9 protein (200 ng/μl) were injected into individual eggs using a FemtoJet and InjectMan NI 2 microinjection system (Eppendorf, Hamburg, Germany). The whole process was completed in two hours ...
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bioRxiv - Biochemistry 2020Quote: ... The rest of the solution was incubated at 4 °C for 1 h on a thermomixer (Eppendorf) set to 1000 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed via centrifugation at 13,000g for 1 hour at +4°C (Eppendorf 5810r centrifuge). A 1 ml HisTrap FF Crude chromatography column (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... Single colonies were grown overnight in 1 mL of SC or SC-ura medium supplemented with 2% glucose in individual wells of the 24-well microtiter plates under constant blue light in an orbital shaker (Eppendorf, New Brunswick, USA) at 30°C and at 200 rpm agitation ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were resuspended and 50 μl of each pre-culture was used to inoculate 1.5 mL of fresh YPD media (corresponding to an optical density of 0.5 at 600 nm) in 96-deep-well plates (LoBind®, 2 mL, cat no. 0030504305, Eppendorf AG, Hamburg, Germany). Plates were covered with an air permeable membrane and incubated while shaking at 300 rpm and 30 °C for 6 hours ...
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bioRxiv - Cell Biology 2021Quote: ... of the same volume as the RIPA buffer at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany). The digested sample was labelled as the RIPA-insoluble fraction (Ins) ...
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bioRxiv - Microbiology 2023Quote: ... algal cells with the attached bacteria in the treatment with the species consortium were also collected by a pipette from the formaldehyde-fixed suspended sample and transferred into 2 ml safe-lock tubes (Eppendorf, Wesseling-Berzdorf, Germany). To avoid an excessive fixation ...
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bioRxiv - Systems Biology 2024Quote: ... placed in a 2mL Protein LoBind® Eppendorf tube and centrifuged at 1000g for 2 minutes at RT (Microcentrifuge 5415, Eppendorf, Hamburg, Germany).
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bioRxiv - Microbiology 2024Quote: ... by five 20 second pulses at speed 4350 rpm with chilling on ice for 2 min between the cycles and clarified by centrifugation at 21,300 rcf for 20 min (Eppendorf 5425R, rotor FA-24x2). Clarified lysates were normalized to 1500 ng/µl and loaded onto 10 – 40% sucrose gradients in gradient buffer and run for 3 hours at 30,000 rpm at 4°C in an SW-41Ti rotor ...
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Identification of ABHD6 as a regulator of lysophosphatidylserines in the mammalian liver and kidneysbioRxiv - Biochemistry 2024Quote: ... 50 μL of 1mg/mL soluble or membrane proteomic fraction from mammalian cells or tissues were incubated with 2 μM FP-rhodamine for 45 minutes at 37°C with constant shaking (Eppendorf, Thermo Mixer C). The reactions were quenched by adding 20 μL of 4x SDS-PAGE loading buffer followed by boiling for 5 minutes ...
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bioRxiv - Physiology 2022Quote: ... plasma was spun at 4°C (4 min, 4000g force, Eppendorf 5804R, Mississauga, ON), decanted and stored at −80°C for subsequent ELISAs ...
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bioRxiv - Biophysics 2021Quote: ... The bicelle mixture (~1 mL) was centrifuged (13,400 rpm, Eppendorf F45-12-11 rotor, 4°C, 30 sec) to remove insoluble debris and the supernatant concentrated to ~200 μL using a 0.5 mL 10 kDa MWCO centrifugal filter (Amicon ...
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bioRxiv - Cell Biology 2022Quote: ... The mixture was shaken for 1 hour at 4°C and 1400 rpm in a Thermomixer (Eppendorf, Germany). The samples were spun down at 14000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... with shaking at 1,200 RPM and 4° C for 1 h using a temperature-controlled Thermomixer 22331 (Eppendorf). The beads were pelleted at 500 x g for 1 min and the supernatant removed to become the unbound fraction ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA (1-5 μg/μl) was microinjected into the fourth ventricle of the embryos (FemtoJet; Eppendorf). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... The 15 mL polypropylene tubes were replaced with 1% BSA-blocked 5 mL Protein LoBind tubes (Eppendorf). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... and denatured at 85°C for 7 minutes using a ThermoMixer® C-PCR 384 (Eppendorf). After denaturation ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 % CO2 (Eppendorf). Jurkat CD4-KO cells were derived from wild-type Jurkat T cells (clone E6 ...
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bioRxiv - Biophysics 2020Quote: 5) Centrifuge (Eppendorf, 5810R)
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bioRxiv - Developmental Biology 2021Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using the FemtoJet 4i (Eppendorf, Hamburg, Germany; Cat. No. 5252000013) micro-injector ...
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bioRxiv - Cell Biology 2020Quote: ... This solution was centrifuged at 3000g for 2 min and the supernatant was collected and concentrated in a vacuum concentrator in vacuum-alcoholic (V-AL) mode (Eppendorf plus, Eppendorf, Hamburg, Germany). The concentrated crystals were resuspended in 100μl of 1 X PBS and Nuclease Free Water (NFW) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were loaded into a microinjection needle and 50 nL of the ZMEL cell suspension was transplanted into the hindbrain ventricle of anesthetized 2 dpf zebrafish larvae by using an oil-controlled microinjection rig (Eppendorf CellTram 4r Oil, #5196000030) with a Narishige arm ...
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bioRxiv - Genomics 2024Quote: ... 2 µL sheared DNA was used to perform overnight pulse-field gel electrophoresis while the remaining sheared DNA was stored in a 2 mL DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Each flask was aeriated with 2% v/v CO2 enriched air which was controlled by a DASGIP® MX module (Eppendorf AG, Hamburg, Germany). The microalgae starting with an OD750 of 0.1 were cultivated in modified Johnson medium at a pH of 7.5 [49] with 1 M NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... Transwells® were then incubated at 37 °C and 25 RPM for 2 hrs in a New Brunswich™ Innova® 40 shaker (Eppendorf, Enfield, CT). Samples were collected every hour from the basolateral side ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 times for 5 seconds each to ensure homogenization and centrifuged at 14000 x g for 10 minutes at 4° C (Eppendorf centrifuge 5424 R, FA-45-24-11 rotor). Supernatant was precleared by rotating 20 µl Protein A agarose beads (Cell Signaling #9863 ...
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bioRxiv - Biochemistry 2022Quote: ... The rest of the solution was incubated at 4°C for 1 hour on a thermomixer (Eppendorf, Hamburg, Germany) set to 1000 rpm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The sample was subsequently centrifuged for 1 h at 11,000 x g at 4°C in an Eppendorf tabletop centrifuge 5810R (Eppendorf). The phage pellet was resuspended in 400 μl DNAseI buffer (10 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... we centrifuged 1 mL overnight-cell culture in a 1.5 mL Eppendorf tube at 7’500 rcf for 5 min (Eppendorf, tabletop centrifuge MiniSpin plus with rotor F-45-12-11 ...
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bioRxiv - Neuroscience 2021Quote: ... starting from 100 ng of total RNA (RIN ≥7) on an epMotion® 5075 TMX workstation (Eppendorf). Library QC included size distribution check (BioAnalyser ...
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bioRxiv - Biochemistry 2020Quote: ... polar phase were transferred into a new 2 mL reaction tube and dried in a vacuum concentrator (Eppendorf, Concentrator plus, mode: V-AQ, 30 °C) for approximately 4.5 h ...
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bioRxiv - Neuroscience 2024Quote: ... 100 µm sections of the BLA were collected in pre-chilled 2 mL DNAase and RNAase-free microcentrifuge tubes (Eppendorf Protein LoBind Tube, Cat #22431102) for a total of 70-100 mg of tissue and stored at -80°C until nuclear isolation.
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bioRxiv - Neuroscience 2020Quote: ... and rotor (Eppendorf #S-4-72). Upon end of centrifugation ...
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bioRxiv - Plant Biology 2021Quote: ... 4 C (Eppendorf Centrifuge 5418 R). Proteins in the clear supernatant (protein extract ...
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bioRxiv - Plant Biology 2021Quote: ... 4 C (Eppendorf Centrifuge 5418 R). 15 µg of recombinant FAMOSS-streptavidin/streptavidin was added to supernatant and incubated 1h on ice with rotation ...
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bioRxiv - Synthetic Biology 2024Quote: ... in a MasterCycler RealPlex 4 (Eppendorf). Each 20 μL qPCR reaction contained 90 ng of cDNA ...
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bioRxiv - Biophysics 2023Quote: ... with a micromanipulator InjectMan 4 (Eppendorf). The injection needles Femtotip II (Eppendorf ...
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bioRxiv - Bioengineering 2024Quote: ... 4 °C (Eppendorf, Centrifuge 5810 R). Next ...
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bioRxiv - Genomics 2022Quote: ... The beads were then incubated 1 h with the anti-Myc antibody (9E10) at 4°C (Eppendorf ThermoMixer, 1,300 rpm), washed three times in PBS/0.1 % BSA and two times in lysis buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: 15mL of a Salten solution (1011 PFU/mL) was distributed into 1.5mL polypropylene tubes and centrifuged for 1 hour at 16,000g at 4°C (Eppendorf Centrifuge 5415R). Pellets were resuspended in 100µL of 100 mM of ammonium acetate (NH4-CH3CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... 5 % CO2 using microloaders (Eppendorf). Slices were placed into the incubation chambers one at a time (minimum volume 1.5 ml to cover raised grid) ...
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bioRxiv - Genomics 2023Quote: ... and centrifuged at 3,200 g and 4°C using a swinging-bucket rotor (Eppendorf A-4-81) for 5 mins ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of the lipid-containing lower organic phase was spotted on a MALDI target plate (Prespotted AnchorChip 96 Set for Proteomics II; Bruker Daltonics/Eppendorf; washed peptide-free with 2-propanol) pre-spotted with 1 µl of 9-aminoacridine (10 mg/ml dissolved in acetone:water 9:1 (v/v) ...
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bioRxiv - Bioengineering 2020Quote: ... and microbubbles were isolated by 4 centrifugation wash cycles at 40 relative centrifugation force for 1 min (Eppendorf 5804, Hamburg, Germany), where the infranatant was discarded and the microbubble concentrate was saved and resuspended.
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bioRxiv - Microbiology 2024Quote: ... The crude EV fractions were prepared by ultracentrifugation (45,000 rpm, 1 h, 4°C; S50A rotor, Himac Ultracentrifuge CS100GXII; Eppendorf Himac Technologies) of the thus prepared supernatants.