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Citations for Eppendorf :
501 - 550 of 638 citations for 6 Chloro 5 fluoro 1H indole 2 carboxylic acid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... cleared by centrifugation and transferred to a clean 2 ml 96-well deep-well plate (DWP, Eppendorf, Hamburg, Germany). TiO2 beads (Titansphere® Phos-TiO Bulk 10 µm ...
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bioRxiv - Cell Biology 2023Quote: ... Oocytes were then microinjected with ~5 pl of mRNAs in M2 medium with 2.5 mM milrinone and 3mg/mL BSA at room temperature with a micromanipulator TransferMan NK 2 (Eppendorf) and picoinjector (Medical Systems Corp.) ...
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bioRxiv - Genomics 2022Quote: ... Supernatant was transferred using a wide-bore pipette to a 2 mL DNA low-bind tube (Eppendorf, Enfield CT), precipitated with 1 mL of 100% ethanol ...
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bioRxiv - Neuroscience 2023Quote: ... and tubes centrifuged at 4 °C for 2 min at 12000 x g in a refrigerated microfuge (Eppendorf 5415R). The upper phase was discarded ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was incubated at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany) to reduce viscosity ...
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bioRxiv - Biochemistry 2023Quote: ... Protein LoBind® microtubes (2 mL) and epT.I.P.S.® pipette tips (1000 µL) were purchased from Eppendorf (Hamburg, Germany).
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bioRxiv - Genomics 2024Quote: ... The resultant DNA was collected and stored in a 2 mL DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C until library preparation ...
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bioRxiv - Microbiology 2019Quote: ... and extension at 72°C for 1 min followed by final extension was conducted at 72°C for 5 min using a PCR minicycler (Eppendorf Ltd., Germany). After amplification ...
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bioRxiv - Developmental Biology 2021Quote: ... the heads were cleaned with alcohol and a 1μl single cell suspension (5 x 106 cells/ml) was manually injected directly into the left cerebellar hemisphere using a glass microcapillary (Eppendorf, 5195 000.079) controlled with an Eppendorf CellTram Vario manual microinjector ...
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bioRxiv - Microbiology 2020Quote: ... The overnight culture was diluted in Mueller Hinton Broth (MHB) based on the OD600 to obtain 5 × 105 CFU/mL using a BioPhotometer D30 (Eppendorf, Hauppauge, NY). An OD600 of 1 was assumed to be equal to 8 × 108 CFU/mL.
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bioRxiv - Cancer Biology 2020Quote: ... sonicated for 15 min and vigorously mixed at 5°C for 30 min with 2000 rpm on a ThermoMixer (Eppendorf, Hamburg, Germany). Samples were centrifuged for 5 min at 18000 rpm ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We separated the tissue from the buffer and reproductive fluid by centrifuging at 4ºC for 5 min at 3500 rpm (Eppendorf Centrifuge 5702RH, USA), pipetted the supernatant from each tract region into microplate wells (CellStar 96 Well Cell Culture Plate ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were pelleted by centrifugation at approximately 250 × g for 5 min at 4 °C (Eppendorf 5804 R, Hamburg, Germany). After resuspension ...
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bioRxiv - Microbiology 2020Quote: ... we centrifuged a 0.5-mL sample of culture in the exponential phase at 7000 rpm for 5 min in a Benchtop centrifuge (5424 Eppendorf, Hamburg, Germany). We washed the pelleted biomass 1 – 2 times and resuspended it with 50 µL 1x PBS from which we fixed 2 µL on solidified agarose (VWR ...
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bioRxiv - Developmental Biology 2019Quote: ... Plasmids used in initial identification screen for cis-regulatory elements used miniprep scale DNA purification (Zymo Research, D4020 or 5 prime/Eppendorf, FastPlasmid kit) and all subsequent experiments used midiprep scale DNA purification (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... then harvested with a sterile cell scraper while at -20°C and transferred to −20 °C cold 5 mL centrifuge tubes (Eppendorf Lo-Bind). After centrifuging the cell-extracts in a 4 °C centrifuge for 5 min at 2000 x g ...
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bioRxiv - Biochemistry 2022Quote: ... The resin was pelleted down by centrifugation at 4°C (1258 g for 5 min, A-4-81 rotor, Eppendorf, Enfield, CT) and washed with ice-cold 50 mL Binding buffer composed of 10mM imidazole (pH 7.4) ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial mixtures were diluted 1:1000 into LB with or without 1% DMSO (v/v) and incubated standing at 37°C in 5% CO2 at atmospheric oxygen (normoxic; Eppendorf CellXpert incubator), 1% oxygen (hypoxic ...
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bioRxiv - Cancer Biology 2023Quote: For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
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bioRxiv - Genomics 2023Quote: ... The bone pieces in 5 ml tube of DNA Lobind were treated pre-digestion for 15 minutes at 900 rpm in a Thermomixer (Eppendorf, Hamburg, Germany) with the lysis buffer (2 ml ...
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bioRxiv - Developmental Biology 2023Quote: ... the gRNA:Cas9 ribonucleoprotein complex solution was incubated at 37°C for 5 min and then backfilled into 3 microinjection needles using an Eppendorf GELoader tip (Eppendorf, Cat# 022351656). After loading embryos into the embryo holder which covered with 12.5 ppt of salinity water with 0.0001% of methylene blue ...
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bioRxiv - Microbiology 2023Quote: ... CNW Technologies) using 80% ACN/5% NH4OH and dried at 45°C using a vacuum centrifuge (cat. no. 5305, Eppendorf, Hamburg, Germany). The peptide samples were resolved in 2% ACN/0.1FA for LC-MS analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were maintained between 10% and 80% confluence and kept at 37 °C with 5% CO2 (8% CO2 for VPC cells) in a humidified CO2 incubator (Eppendorf, Enfield, CT). The viable cell density (VCD ...
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bioRxiv - Microbiology 2024Quote: ... one with the blood mixed to RPMI and the second with the gametocyte suspension were spun down at 2000rpm for 5 minutes in a temperature-controlled centrifuge at 37°C (Eppendorf 5702 R). RPMI supernatant was removed from both tubes with 3ml disposable Pasteur pipettes ...
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bioRxiv - Cell Biology 2020Quote: ... the beads were separated from the protein extracts on a magnetic rack and transferred to a new 2 ml LoBind tube (Eppendorf). The beads were washed with 1 ml of each following solution ...
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bioRxiv - Cell Biology 2020Quote: ... The whole lane was excised from the gel and future cut into approximate 3 x 1 x 2 mm3 slices (L x W x H) and each slice were placed into a separate LoBind tubes (Eppendorf) for destaining ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was eluted with 2 x 100 μl of fresh elution buffer (100 mM dithiothreitol in RNase-free H20) directly into 2 ml lobind tubes (Eppendorf) containing 700μl Buffer RLT (RNeasy MinElute Cleanup Kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Soaked biofilms were gently removed from the agar surface using a sterile Drigalski spatula and a pipette tip and together with the unabsorbed stabilization mix transferred into a 2 mL tube (Eppendorf). An additional 1 mL of stabilization buffer was added to the tubes and the content was homogenized with a sterile pestle ...
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bioRxiv - Developmental Biology 2021Quote: ... the tissue samples were weighed (between 1.4 - 11.0 mg) and transferred into a 2 mL screw cap Eppendorf plastic tubes (Eppendorf, Stevenage, UK) along with a single 5 mm stainless steel ball bearing ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenate was filtered through a 50 m.um filter (Sysmex; 04-004-2327) into a 2 mL microcentrifuge tube (Eppendorf; 022431048). An additional 0.5 mL of homogenization buffer was used to wash the Dounce homogenizer and filter ...
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bioRxiv - Molecular Biology 2019Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 2 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA.
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bioRxiv - Evolutionary Biology 2019Quote: ... pH 8.5 with 1 µg endoproteinase Lys-C (Wako) for 2 hours with shaking at 600 rpm at 37 °C in a thermomixer (Eppendorf). One µg of trypsin (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... The surrounding SCFM was directly transferred to individual sterile 2 ml DNA lo-bind tubes from the 24-well plate (Eppendorf).
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bioRxiv - Biochemistry 2020Quote: ... Reactions were started by addition of 59.0 μM ubiquitin and 2 mM ATP then incubated at 37°C in a Mastercycler nexus (Eppendorf, Germany) thermocycler for 2 hrs unless otherwise stated ...
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bioRxiv - Biochemistry 2020Quote: ... we gently shook the flower and collected pollen into a weighed 2 mL microcentrifuge tube (Eppendorf®, Safe-Lock™). For species for which pollen was more difficult to harvest ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were kept on ice and centrifuged at 14 krpm for 2 min on a mini spin plus centrifuge (Eppendorf) before measurement ...
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bioRxiv - Neuroscience 2019Quote: ... supplemented with 3.5 µl 2-β mercaptoethanol was added directly to the beads and incubated with mixing on a ThermoMixer (Eppendorf) for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μM purified TDP-43-MBP-His6 variants (WT, 5D, 12D, 12A) were set up in low binding tubes (Eppendorf) in 35 μl aggregation buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 15-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C until further analysis ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and sonicated in chilled PBS (1 s on ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was transferred into a new 2 mL Eppendorf tube and evaporated to dryness using a Concentrator Plus (Eppendorf) (V-AL ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were spun at 10,000 g for 15 min (spin 2 in the same Eppendorf FA-45-48-11 rotor) and the resulting supernatant is the tissue lysate (TL ...
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bioRxiv - Immunology 2022Quote: ... For that 2.5x105 cells were incubated with HIV-1 antigens in PBS/2% FCS for the indicated period of times on a 37°C thermomixer (Eppendorf). Cells were then fixed with IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
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bioRxiv - Microbiology 2022Quote: ... Isolated midguts or the mosquito carcass (remainder of the body without midgut) were placed separately into 2 ml safe-lock Eppendorf tubes (Eppendorf) containing 250 µl of mosquito diluent (1 × PBS supplemented with 20% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Annealing was carried out by heating the mixture to 95 °C for 2 minutes followed by gradual cooling to 25 °C over 45 minutes in a thermocycler (Eppendorf).
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bioRxiv - Developmental Biology 2020Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using FemtoJet or FemtoJet 4i (Eppendorf; 5252000013) micro-injectors ...
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bioRxiv - Neuroscience 2020Quote: ... or vehicle for thirteen days from postnatal day 2 (P2) to postnatal day 14 (P14) using a micropipette (0.5-10 µL; Cat. No. 3123000020; Eppendorf, Germany). Mouse pups for each postnatal treatment experiment were selected from at least six different dams to minimize any litter-related bias ...
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bioRxiv - Biochemistry 2021Quote: ... and a concentration of 5 µg / ml of the plasmid with GFP (Green Fluorescent Protein) were transferred to an electroporation cuvette (2 mm wide and 400 µl in volume (Eppendorf)) ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in 1mL of ice-cold PBS and transferred to 2 mL LoBind Eppendorf tubes (Eppendorf #022431102). Cells were centrifuged for 2 minutes at 800rpm before being flash-frozen on dry ice ...