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Citations for Eppendorf :
501 - 550 of 723 citations for 3 Ethyl 3 methyloxolane 2 5 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... The cell suspension was then transferred to a pre-chilled electroporation cuvette and after approximately 5 – 10 min electroporated in the eporator electroporator (Eppendorf) set to 1850 V ...
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bioRxiv - Cell Biology 2023Quote: ... After a 5-minute incubation the protein concentration was determined through measurement of the OD595 with a photometer (BioPhotometer plus, Eppendorf). Finally ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 x 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Neuroscience 2023Quote: Neurons remained in medium and were incubated in ∼1% O2 (5% CO2, 37°C) for 360 min in a triple-gas incubator (Eppendorf) and then returned to normoxic conditions (21% O2 ...
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bioRxiv - Cell Biology 2023Quote: ... is harvested by centrifugation at 300 × g for 5 min in a swing out 5804 S-4-72 rotor (Eppendorf). P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: All cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). They have also been regularly tested for mycoplasma contamination by examining the samples for extracellular DNA staining with SiR-DNA (100 nM ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 and 0.1% Tween 20 in water) and centrifuged for 5 min at 500 g in a swinging bucket rotor centrifuge (5920R, Eppendorf). Nuclei pellets were resuspended in 1X Tagmentation Buffer (10 mM Tris pH 7.4 ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting pellet was re-suspended in 5 mL of modified CIB medium and centrifuged at 16,000 gav for 10 min in 5415R refrigerated centrifuge (Eppendorf, USA). The resulting supernatant was the Sucrose Gradient Load (SGL) ...
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bioRxiv - Neuroscience 2022Quote: ... and Sample Reducing Agent (10x) and incubated for 5 min at 70°C and 1100 rpm using a ThermoMixer comfort (Eppendorf).
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 × 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Developmental Biology 2023Quote: ... were mixed in HEPES-CZB medium containing 5 μg/ml cytochalasin B (CB) and injected into the cytoplasm of fertilized eggs using a FemtoJet microinjector (Eppendorf) with constant flow settings ...
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bioRxiv - Pathology 2023Quote: ... The samples were extracted overnight at 4℃ and then centrifuged at 4,000 rpm for 5 min at 4°C using a 5810 R fixed-angle rotor centrifuge (Eppendorf, Germany). 1 ml of the upper layer of petroleum ether was dried under vacuum in a new 2-ml tube ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in 5 µL of RNase-free water and placed into a clean 1.5 mL Eppendorf DNA LoBind tube (Eppendorf, 30108051). RNA and cDNA were quantified using Qubit™ ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted peptides were fractionated into 5 fractions using strong cation exchange (SCX) StageTips and vaccum-dried while centrifuged in a Speed Vac (Eppendorf).
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bioRxiv - Genomics 2024Quote: ... was added without disturbing the pellet and nuclei were pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). The supernatant was removed without disturbing the pellet and 7-10 µl of 1x Nuclei Buffer (10x Genomics ...
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bioRxiv - Genomics 2024Quote: ... and incubated on ice for 1 minute before pelleting with a swinging-bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). The supernatant was gently removed and ∼50 µl were left behind to increase nuclei recovery ...
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bioRxiv - Cell Biology 2024Quote: ... All cells were kept at 37 °C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). All cell lines have been routinely examined for mycoplasma contamination by using SPY-555-DNA (1:100 000 ...
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bioRxiv - Microbiology 2024Quote: ... The culture was adjusted to OD600 75 by centrifuging 50 mL of culture in a conical tube at 3,220 ×g at room temperature for 5 min in a 5810 R centrifuge (Eppendorf, Germany). The supernatant was removed ...
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bioRxiv - Molecular Biology 2024Quote: ... After The upper lipophilic phases and lower hydrophilic phases were separated by high-speed centrifugation (12,700 rpm, 5 min at 4 °C, Centrifuge 5430R, Eppendorf, Germany). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were then centrifuged (20 k g for 5 minutes) and supernatants (F2) were transferred to LoBind Protein tubes (Eppendorf). Protein concentrations were determined using bicinchoninic acid (BCA ...
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bioRxiv - Cancer Biology 2024Quote: ... EVs-beads conjugates were then incubated with 15 uL EVs detection reagent (5 uL of CD9, CD63, CD81 detection reagents) for 1 hour at room temperature in Thermomixer orbital shaker (Eppendorf). Samples were washed with 500 uL MACSPlex buffer and centrifuged at 3000xg for 5 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... were mixed in HEPES-CZB medium containing 5 μg/ml cytochalasin B (CB) and injected into the cytoplasm of fertilized eggs using a FemtoJet microinjector (Eppendorf) with constant flow settings ...
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bioRxiv - Immunology 2022Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ∼ 0.8 ...
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bioRxiv - Synthetic Biology 2020Quote: ... These reactions were incubated for 2 h at 37°C with 220 rpm shaking (Eppendorf, Thermo Mixer C). AcTEV protease AL-PHA assays ...
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bioRxiv - Synthetic Biology 2020Quote: ... These reactions were incubated for 2 h at 30°C with 500 rpm shaking (Eppendorf, Thermo Mixer C). MMP14 AL-PHA assays ...
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bioRxiv - Bioengineering 2021Quote: ... After finishing every experiment RBCs were pelleted down by centrifuging at 1000 rpm for 2 min (Eppendorf, 5415C) and the supernatant absorbance was assessed and normalized using the maximum hemolysis value.
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bioRxiv - Physiology 2021Quote: ... These were then incubated at 70°C at 1400 rpm for 2 hours on a heat shaker (Eppendorf) and spun at 2500 x g for 5 min ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were thawed shortly before mass spectrometric analysis and shaken for 2 minutes at 2000rpm (thermomixer C, Eppendorf). Peptide concentrations were measured optically at 280nm (Nanodrop 2000 ...
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bioRxiv - Immunology 2020Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ~0.8 ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was hydrolyzed in 3N HCl for 2 hours at 95 °C on a shaking Thermomixer (Eppendorf). The reaction was quenched with 100 % methanol containing 40 μM L-norvaline (as an internal control) ...
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bioRxiv - Cell Biology 2022Quote: U-2 OS cells were grown in 24-well glass bottom plates (170 µm coverglass bottom; Eppendorf #0030741021) coated with poly-l-lysine and treated with 200 µM oleate-BSA complex for 24 hr ...
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bioRxiv - Microbiology 2020Quote: ... mouth rinse-treated SARS-CoV-2 viruses were concentrated by centrifugation at 14,000 rpm in a centrifuge (Eppendorf) at 4°C for 2 h as described previously (Holmes et al ...
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bioRxiv - Cancer Biology 2023Quote: ... and then gently shaken at 300 RPM for 2 hr at 37°C using a Thermomixer C (Eppendorf). Sample pH was then adjusted to 4 with 15 μL of 20% formic acid before loading onto conditioned SPE Bond-Elute pH columns (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... two milliliters of the fermentation broth containing the suspended mucin beads were sampled to 2-ml tubes (Eppendorf). The serum bottles were opened and closed carefully each time to avoid contamination in the anaerobic workstation ...
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bioRxiv - Microbiology 2023Quote: ... 2 μM final test concentration) were mixed (reaction mix) and transferred into a white 96 well plate (Eppendorf Twin-Tec ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pH 7.6) and injected into 1 to 2-cell-stage embryos by using a microinjector FemtoJet 5247 (Eppendorf). The sequences and the concentrations of MOs (Gene Tools ...
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bioRxiv - Biochemistry 2022Quote: ... The collected powder was homogenised and stored in 2 mL protein LoBind tubes (Eppendorf UK Limited, Stevenage, UK) at −80°C until extraction and testing ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 mL subsamples of cell cultures normalized as above were dispensed into 2 mL tubes (cat: 0030120094, Eppendorf) and stained with MitoTracker Orange CMTMRos (cat ...
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bioRxiv - Neuroscience 2022Quote: ... 1-2 fecal pellets were collected from each mouse by voluntary defecation into a sterile microtube (Eppendorf, Germany) and fecal samples were immediately placed on dry ice ...
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bioRxiv - Microbiology 2024Quote: ... The collected sample was concentrated in a SpeedVac (45°C, V-AQ, 1-2 hrs) (Eppendorf Concentrator 5301). The samples were stored at 20°C until LC-MS measurement.
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bioRxiv - Biochemistry 2021Quote: ... After suspension it was subjected to centrifugation for 5 minutes at 5000 rpm in an Eppendorf 5804R centrifuge (Eppendorf, Hamburg, Germany) after which the supernatant was decanted into the waste ...
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bioRxiv - Bioengineering 2021Quote: ... CsupADH_17286 and HzeaADH7 were introduced into Agrobacterium tumefaciens GV3101 strain (MP90RK) by electroporation (1700 V mm-1, 5 ms, Eppendorf 2510). A viral silencing suppressor protein P19 was introduced into GV3101 strain as well in order to inhibit the host cells’ transgene silencing apparatus and extend transgene expression over a longer period of time with a higher degree of expression (Canto et al ...
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bioRxiv - Biochemistry 2022Quote: ... After 5 min incubation at room temperature (RT) the sample was centrifuged (8 min, RT, 180 x g, Eppendorf centrifuge 5810R) to separate proteoliposomes from the non-incorporated protein and detergent ...
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bioRxiv - Microbiology 2020Quote: ... and 100 U/mL penicillin/streptomycin (Himedia, Mumbai India) under conditions of 5% CO2 and humidified air at 37°C (Eppendorf India).
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bioRxiv - Microbiology 2021Quote: ... overnight cultures in LB were spun down via centrifugation for 5 minutes at 4000 rpm at room temperature (Eppendorf 5810 R). The supernatant was filtered twice (Medical Millex-GS Filter ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were dislodged from a T75 flask and pelleted via centrifugation at 1300 rpm for 5 minutes at 20 °C (Eppendorf 5804R). The supernatant was aspirated ...
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bioRxiv - Neuroscience 2023Quote: ... Following that, the cells were washed thrice with 1mL Dulbecco’s DPBS and centrifugation (600 ⍰ g, 5 min, room temperature)(Eppendorf, cat # 5424R). Afterwards ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were washed with cold 70% ethanol and centrifuged at 19,000 × g for 5 min at 4°C and air dried for 15 min in the Centrifugal Vacuum Concentrator 5301 (Eppendorf, USA). The quality of RNA was assessed using a NanoDrop (ND-1000 ...
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bioRxiv - Microbiology 2024Quote: ... 50 mL culture was centrifuged for 5 min at 18 °C and 3,220 g (5810 R swing-out centrifuge; Eppendorf, Hamburg, Germany) and the supernatant was removed by vacuum suction ...
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bioRxiv - Microbiology 2024Quote: ... The stationary pre-cultures were then pooled and washed by centrifugation (5 min, 3,220 g and 4 °C) using a 5810 R swing-out centrifuge (Eppendorf, Hamburg, Germany). After centrifugation ...