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Citations for Eppendorf :
501 - 550 of 773 citations for 3' Methyl 1 1' biphenyl 4 amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... were diluted in BME (1:10) and locally injected into the outer SVZ by using a FemtoJet® 4i microinjector (Eppendorf). After 72 h ...
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bioRxiv - Microbiology 2022Quote: A lysate sample (1 ml) with 108 PFU/ml was centrifuged at 20,000 g (centrifuge 5430R, rotor FA-45-24-11HS; Eppendorf, Hamburg Germany) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the injection needle was loaded with the injection mixture calibrated using a microscale graticule to deliver 1-1.7nl per injection and each egg was injected (FemtoJet 4x, Eppendorf, Hamburg, Germany), once ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of MTBE was added and the solution was incubated for 1 hour at room temperature with continuous shaking at 600 rpm (Eppendorf, MixMate). Next ...
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bioRxiv - Synthetic Biology 2023Quote: ... then 1 mL aliquots were placed in triplicate in plastic-bottomed black 24-well plates (Eppendorf Cell Imaging Plates ref. 0030741005), and the plates were placed onto a custom made light plate apparatus (LPA)27 to control blue light illumination in each well (λ = 460 nm ...
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bioRxiv - Neuroscience 2023Quote: ... was added to lyophilized Aβ1-42 peptide to a final concentration of 1 mM and centrifuged in a speed vac (Eppendorf, Hamburg, Germany). The peptide pellet was re suspended in DMSO at a 5 mM concentration and sonicated in a 37⁰C water bath for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... by adding 50 μL of a 2 mg/mL stock solution and 25 μL of 50 mM CaCl2 and incubating for 1 h at 37 °C with agitation at 300 rpm in a Thermomixer comfort (Eppendorf, Germany). The cell wall material was pelleted and washed two times with 2 mL of ultrapure water (21000 g ...
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bioRxiv - Biochemistry 2023Quote: ... The cellulase-LPMO synergy was assessed by performing reactions with crystalline Avicel (1 % w/v) in 50 mM sodium phosphate buffer (pH 6.0) using a thermomixer (Eppendorf, Hamburg, Germany) incubated at 30 °C with horizontal agitation (1000 rpm) ...
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bioRxiv - Cell Biology 2023Quote: ... or at 37°C/5% CO2/1% O2 in a nitrogen-controlled hypoxic incubator (New Brunswick Galaxy 170R, Eppendorf, Hamburg, Germany). Prior to media changes ...
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bioRxiv - Biochemistry 2023Quote: ... was adjusted to 50 ng/μL with 50% ACN containing 0.1% TFA and dispensed in 10 μL each into normal tubes (1.5-mL safe-lock tube, CAT# 0030120086, Eppendorf, Hamburg; Germany), normal polypropylene (PP ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... A 1 ml sample of overnight suspension was centrifuged for five minutes at 8,000 rpm (Eppendorf MiniSpin F-45-12-11), the pellet resuspended in 1 ml PBS ...
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bioRxiv - Physiology 2024Quote: Protein samples were dissolved in 8 M urea in 50 mM ammonium bicarbonate (pH 7.8) by vortexing for 1 hour (Eppendorf ThermoMixer F1.5). 5 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of 108 PFU was centrifuged at 20,000 g (centrifuge 5430 R, rotor FA- 45-24-11HS; Eppendorf, Hamburg, Germany) for 2h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of formic acid 1% were placed in each Millipore Microcon 30 MRCFOR030 Ultracel PL-30 before centrifugation at 14,500 rpm (Eppendorf 5424 centrifuge) for 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of 108 PFU was centrifuged at 20,000 g (centrifuge 5430 R, rotor FA-45-24-11HS; Eppendorf, Hamburg, Germany) for 2h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... in PBS in a 15mL tube and spun at 1000g for 10 min at room temperature with acceleration and brake set to 1 (Eppendorf 5810R). The supernatant was discarded ...
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bioRxiv - Microbiology 2021Quote: ... 0.5-1 mL culture sample was harvested by centrifugation for 3 min at 13806 rpm (Centrifuge 5424, FA-45-24-11, Eppendorf) and resuspended in 100-500 µL 10 mM NaOH depending on the size of the cell pellet ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 15-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C until further analysis ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and sonicated in chilled PBS (1 s on ...
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bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 30 mycn mutant or WT zebrafish embryos at 3 dpf were transferred to 1.5mL Low binding microcentrifuge tubes (Eppendorf 022431021). Trypsin-EDTA solution (Beyotime ...
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bioRxiv - Microbiology 2021Quote: ... was performed with 0.5 µg of ligated DNA with oligonucleotides 6212 and 6420 in a standard PCR program with a 3 min extension time on a Mastercycler thermocycler (Eppendorf). Phusion DNA polymerase (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation reactions were carried out for the indicated proteins using rabbit reticulocyte lysate and SP cells at a final concentration of 3×106 cells/ml at 30°C for 40 min in a thermomixer (Eppendorf) set to 900 rpm ...
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bioRxiv - Physiology 2020Quote: ... with 3 mM Tris(2-carboxyethyl)phosphine hydrochloride(TCEP-HCl) (Thermo Pierce) while shaking at 600 rpm in a thermomixer (Eppendorf). Samples were alkylated with 9 mM iodoacetamide (22 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transferred to a PCR-strip tube and heated for 3 min using a Mastercycler Nexus X2 thermal cycler (Eppendorf). After heating ...
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bioRxiv - Molecular Biology 2023Quote: ... the rehydrated gel pieces were overlaid with 30 μL of 0.01% ProteaseMAX in 50 mM ammonium bicarbonate and incubated at 37°C for 3 hours with shaking at 800 rpm (Thermomixer, Eppendorf). The digestion reaction was transferred to a fresh tube ...
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bioRxiv - Neuroscience 2023Quote: ... PFF oligomers were incubated with or without SVD-1a in PBS pH 7.4 for 3 d at 37 °C under quiescent condition in low retention Eppendorf tubes (Eppendorf, GE). Prior to injection ...
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bioRxiv - Microbiology 2023Quote: ... The beads were pelleted (600 x g, 3 min) and the supernatant transferred to a fresh low protein binding tube (Eppendorf). The beads were washed with 1 x 200 µL HPLC-grade water and the washes combined with the original supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were eluted with 60 μl of 80% acetonitrile/1% ammonia and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were dissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Microbiology 2021Quote: ... Bacillus cereus group isolates’ inocula were prepared from overnight cultures (see “Bacterial cultures” section) by adjusting their concentration to 1-2 × 108 CFU/ml using a spectrophotometer (Eppendorf BioPhotometer 6131). A previously established OD-CFU standard curve was used to estimate the CFU/ml based on the OD reading ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were eluted with 60 μl of elution buffer (80% acetonitrile/1% ammonia) and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were redissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Biophysics 2022Quote: ... The cultures were then induced with IPTG by adding 10 uL of 100 mM (final concentration approximately 1 mM) per well with an electric repeater pipette (Eppendorf, E4x series), and grown for another 4 h at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/ml of hygromycin and correct genome editing was confirmed by Sanger sequencing (Macrogen Europe ...
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bioRxiv - Developmental Biology 2020Quote: ... Microinjection of young adult hermaphrodite germlines was done using an inverted microinjection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). In cases where the sgRNA target site was not disrupted by sequence integration ...
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bioRxiv - Microbiology 2022Quote: ... 4.6×106 LMBs or NMBs were added and samples incubated at 37 °C for 1 hour on a thermomixer (60 rpm; Eppendorf, Hamburg, Germany). After this step ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Biochemistry 2022Quote: ... samples were prepared in 96 well microtiter plates by adding 12.5 μL reaction supernatant to 50 μL acetonitrile with 1% v/v trifluoroacetic acid (TFA) followed by centrifugation (2,204 g, 30 min; A-2-DWP rotor, Eppendorf AG, Hamburg, Germany) and transferring of 50 μL centrifugation supernatant into 150 μL MilliQ H2O ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmid injection solution was injected into Hydra vulgaris AEP 1-cell stage embryos using an Eppendorf FemtoJet 4x and Eppendorf InjectMan NI 2 microinjector (Eppendorf; Hamburg, Germany) under a Leica M165 C stereo microscope (Leica Microscopes ...
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bioRxiv - Systems Biology 2024Quote: ... are then added to the sample and incubated at 56°C for 1 hour at 1000 RPM (ThermoMixer C, Eppendorf, Hamburg, Germany). 37µl of 500mM chloroacetamide (50mM final concentration ...
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bioRxiv - Genetics 2024Quote: ... placed in an injection arena (9 cm-diameter Petri dish containing a layer of 1% agarose) and injected with ∼60 ng of Pmtra-2 or EGFP dsRNA (negative control) using a FemtoJet microinjector (Eppendorf, Hamburg, Germany). Injectees were maintained in a two-ounce plastic cup with corn leaves until they became adults ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial mixtures were diluted 1:1000 into LB with or without 1% DMSO (v/v) and incubated standing at 37°C in 5% CO2 at atmospheric oxygen (normoxic; Eppendorf CellXpert incubator), 1% oxygen (hypoxic ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 1800 µl of solution from both tubes (batch 1) were transferred to a 2 ml Eppendorf and vortexed (Eppendorf Thermomixer C) for 15 minutes at 2000 rpm (4 °C) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng corresponding pEcgRNA-N20 and 400 ng donor DNA were co-electrotransformed into 100 uL competent cells in 1 mm gap electroporation cuvette by applying 2.5 kV pulse in electroporator (Eppendorf™ Eporator™). After adding 900 uL of cold SOC medium ...
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bioRxiv - Biochemistry 2024Quote: ... A second gDNA purification was coupled with reverse transcription of RNA (1 μg) into cDNA with the SuperScript IV VILO ezDNase system (Thermo Fischer Scientific) using an Eppendorf Mastercycler (Eppendorf, Hamburg, Germany). cDNA samples were entered into a custom TaqMan low density array (Thermo Fischer Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... castellanii trophozoites were seeded at the log growth phase (1 × 104 cells per well) in a 96-well white microplate (Eppendorf, Hamburg, Germany). Incubation with medium containing varied nitroxoline concentrations ranging from 0 to 100 μM was performed ...
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bioRxiv - Neuroscience 2024Quote: ... Lyophilized peptide was dissolved in 100% HFIP solvent to a final concentration of 1 mM and centrifuged in a speed-vac (Eppendorf, Hamburg, Germany). Peptide pellet was then resuspended in DMSO to a final concentration of 5 mM followed by sonication at 37°C for 10 minutes ...
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bioRxiv - Zoology 2024Quote: ... and sgRNA (150 ng/μL) was co-injected into embryos (<1 hr-old) using the InjectMan NI 2 microinjection system (Eppendorf, Hamburg, Germany). Injected eggs were incubated at 26 ± 1 °C and 70% ± 5% relative humidity for hatching.