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Citations for Eppendorf :
451 - 500 of 688 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: Protein samples were dissolved in 8 M urea in 50 mM ammonium bicarbonate (pH 7.8) by vortexing for 1 hour (Eppendorf ThermoMixer F1.5). 5 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of 108 PFU was centrifuged at 20,000 g (centrifuge 5430 R, rotor FA- 45-24-11HS; Eppendorf, Hamburg, Germany) for 2h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of formic acid 1% were placed in each Millipore Microcon 30 MRCFOR030 Ultracel PL-30 before centrifugation at 14,500 rpm (Eppendorf 5424 centrifuge) for 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of 108 PFU was centrifuged at 20,000 g (centrifuge 5430 R, rotor FA-45-24-11HS; Eppendorf, Hamburg, Germany) for 2h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... in PBS in a 15mL tube and spun at 1000g for 10 min at room temperature with acceleration and brake set to 1 (Eppendorf 5810R). The supernatant was discarded ...
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bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were eluted with 60 μl of 80% acetonitrile/1% ammonia and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were dissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Microbiology 2021Quote: ... Bacillus cereus group isolates’ inocula were prepared from overnight cultures (see “Bacterial cultures” section) by adjusting their concentration to 1-2 × 108 CFU/ml using a spectrophotometer (Eppendorf BioPhotometer 6131). A previously established OD-CFU standard curve was used to estimate the CFU/ml based on the OD reading ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were eluted with 60 μl of elution buffer (80% acetonitrile/1% ammonia) and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were redissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Biophysics 2022Quote: ... The cultures were then induced with IPTG by adding 10 uL of 100 mM (final concentration approximately 1 mM) per well with an electric repeater pipette (Eppendorf, E4x series), and grown for another 4 h at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/ml of hygromycin and correct genome editing was confirmed by Sanger sequencing (Macrogen Europe ...
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bioRxiv - Developmental Biology 2020Quote: ... Microinjection of young adult hermaphrodite germlines was done using an inverted microinjection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). In cases where the sgRNA target site was not disrupted by sequence integration ...
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bioRxiv - Microbiology 2022Quote: ... 4.6×106 LMBs or NMBs were added and samples incubated at 37 °C for 1 hour on a thermomixer (60 rpm; Eppendorf, Hamburg, Germany). After this step ...
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bioRxiv - Developmental Biology 2021Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Young adult hermaphrodites were injected in the germline using an inverted micro-injection setup (Eppendorf FemtoJet 4x mounted on a Zeiss Axio Observer A.1 equipped with an Eppendorf Transferman 4r). Candidate edited progeny were selected on plates containing 250 ng/μl of hygromycin (Dickinson et al. ...
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bioRxiv - Biochemistry 2022Quote: ... samples were prepared in 96 well microtiter plates by adding 12.5 μL reaction supernatant to 50 μL acetonitrile with 1% v/v trifluoroacetic acid (TFA) followed by centrifugation (2,204 g, 30 min; A-2-DWP rotor, Eppendorf AG, Hamburg, Germany) and transferring of 50 μL centrifugation supernatant into 150 μL MilliQ H2O ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmid injection solution was injected into Hydra vulgaris AEP 1-cell stage embryos using an Eppendorf FemtoJet 4x and Eppendorf InjectMan NI 2 microinjector (Eppendorf; Hamburg, Germany) under a Leica M165 C stereo microscope (Leica Microscopes ...
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bioRxiv - Systems Biology 2024Quote: ... are then added to the sample and incubated at 56°C for 1 hour at 1000 RPM (ThermoMixer C, Eppendorf, Hamburg, Germany). 37µl of 500mM chloroacetamide (50mM final concentration ...
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bioRxiv - Plant Biology 2024Quote: ... SDS at a 1:3 (w/v) ratio and extracted by shaking 1000 RPM 95 °C for using a tabletop shaker (Eppendorf ThermoMixer F2.0). Samples were then centrifuged at 20,000 xg for 10 min at room temperature and the supernatant retained in new tubes ...
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bioRxiv - Genetics 2024Quote: ... placed in an injection arena (9 cm-diameter Petri dish containing a layer of 1% agarose) and injected with ∼60 ng of Pmtra-2 or EGFP dsRNA (negative control) using a FemtoJet microinjector (Eppendorf, Hamburg, Germany). Injectees were maintained in a two-ounce plastic cup with corn leaves until they became adults ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial mixtures were diluted 1:1000 into LB with or without 1% DMSO (v/v) and incubated standing at 37°C in 5% CO2 at atmospheric oxygen (normoxic; Eppendorf CellXpert incubator), 1% oxygen (hypoxic ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 1800 µl of solution from both tubes (batch 1) were transferred to a 2 ml Eppendorf and vortexed (Eppendorf Thermomixer C) for 15 minutes at 2000 rpm (4 °C) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 ng corresponding pEcgRNA-N20 and 400 ng donor DNA were co-electrotransformed into 100 uL competent cells in 1 mm gap electroporation cuvette by applying 2.5 kV pulse in electroporator (Eppendorf™ Eporator™). After adding 900 uL of cold SOC medium ...
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bioRxiv - Biochemistry 2024Quote: ... A second gDNA purification was coupled with reverse transcription of RNA (1 μg) into cDNA with the SuperScript IV VILO ezDNase system (Thermo Fischer Scientific) using an Eppendorf Mastercycler (Eppendorf, Hamburg, Germany). cDNA samples were entered into a custom TaqMan low density array (Thermo Fischer Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... castellanii trophozoites were seeded at the log growth phase (1 × 104 cells per well) in a 96-well white microplate (Eppendorf, Hamburg, Germany). Incubation with medium containing varied nitroxoline concentrations ranging from 0 to 100 μM was performed ...
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bioRxiv - Neuroscience 2024Quote: ... Lyophilized peptide was dissolved in 100% HFIP solvent to a final concentration of 1 mM and centrifuged in a speed-vac (Eppendorf, Hamburg, Germany). Peptide pellet was then resuspended in DMSO to a final concentration of 5 mM followed by sonication at 37°C for 10 minutes ...
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bioRxiv - Zoology 2024Quote: ... and sgRNA (150 ng/μL) was co-injected into embryos (<1 hr-old) using the InjectMan NI 2 microinjection system (Eppendorf, Hamburg, Germany). Injected eggs were incubated at 26 ± 1 °C and 70% ± 5% relative humidity for hatching.
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bioRxiv - Neuroscience 2020Quote: ... Solutions were exchanged by a low speed centrifuge step (4’ at 800 rpm in table top Eppendorf centrifuge) and aspiration ...
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bioRxiv - Synthetic Biology 2020Quote: ... These reactions were incubated for 4 h at 37°C with 500 rpm shaking (Eppendorf, Thermo Mixer C). HEK293 conditioned media AL-PHA assays ...
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bioRxiv - Synthetic Biology 2020Quote: ... These reactions were incubated for 4 h at 37°C with 500 rpm shaking (Eppendorf, Thermo Mixer C). EV-associated ADAM10 AL-PHA assays ...
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bioRxiv - Physiology 2020Quote: Plasma was obtained by centrifugation (4°C, 1820 x g for 30 min; Eppendorf 5810 R, Hamburg, Germany) and stored in aliquots at −20°C until insulin and metabolites (glucose ...
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bioRxiv - Biophysics 2021Quote: Injection of ferritin MNPs into cells was carried out using the InjectMan 4 and FemtoJet 4i systems (Eppendorf). To this end ...
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bioRxiv - Immunology 2021Quote: ... The obtained lysates were centrifuged for 20 min at 21,000 g and 4°C (Centrifuge 5424 R, Eppendorf) to precipitate insoluble fractions ...
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bioRxiv - Genetics 2021Quote: ... Filtered nuclei were pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf) and resuspended in 1 mL Wash buffer (10mM Tris-HCL (pH 7.5) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were pelleted by centrifugation (3220 x g 5 min, Eppendorf 5810R fitted with rotor A-4-81), pellets were recombined with 5 mL pre-chilled MP medium (no carbon) ...
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bioRxiv - Molecular Biology 2021Quote: ... 55S mitoribosomes then underwent a clearance spin at 14000 rpm and 4°C (tabletop centrifuge 5427 R, Eppendorf). We realized that our mitoribosomes contained endogeneous tRNAs in the A and P sites of the ribosome ...
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bioRxiv - Synthetic Biology 2024Quote: ... Aeration of the chemostat was done through a DASGIP MX4/4 stand-alone gas-mixing module (Eppendorf, Germany) with a composition of 10% CO2 and 5% oxygen and 85% air at a flow rate of 40 sL/hr ...
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bioRxiv - Molecular Biology 2023Quote: ... Yeast were centrifuged at 3,000 rpm for 3 min in an A-4-62 swing bucket rotor (Eppendorf) and resuspended in 20 ml 1 M sorbitol and incubated at 4 °C overnight (<18 h) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were centrifuged at 20,000× g for 30 min at 4 °C (5804/5804 R Centrifuge, Eppendorf, USA), and supernatants were discarded ...
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bioRxiv - Genomics 2023Quote: ... The DNA solution was pelleted by centrifugation at 6,000xg for 5 minutes at 4°C (Eppendorf Centrifuge 5425R). After removing the supernatant ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were centrifuged at 4000 rpm × 5 min at 4°C in a pre-cooled microcentrifuge (Eppendorf 5415R) and the supernatant was collected ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were then extracted for 3 hr at 4°C in a ThermoMixer (Eppendorf US, Enfield, CT, USA), followed by evaporation of the methanol component with a speedvac concentrator (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were centrifuged down at 11,200 g for 30 min at 4°C (Eppendorf Centrifuge 5415 R). The supernatant was collected and transferred to a fresh microfuge tube ...
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bioRxiv - Physiology 2024Quote: ... LPDP was clotted with 1U/ml thrombin and centrifuged (Eppendorf, S-4-104, 3K rpm, 4oC, 15 min). Radiolabeled [3H]-triolein (NET431991MC Revvity ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were collected by centrifugation at 4,000 ×g and 4 °C for 15 min (5804R centrifuge, Eppendorf), washed with 50 mM Tris-HCl buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Production of CjLPMO10AFL was achieved by fed-batch fermentation of the expression strain in a 1-liter fermenter (DASGIP® benchtop bioreactors for cell culture; Eppendorf, Hamburg, Germany), essentially as described previously (44) ...
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bioRxiv - Microbiology 2020Quote: ... printed arrays were moved to individual 1 mL centrifuge tubes containing 200 μL of M9 medium and melted at 55°C for 1 min in a thermomixer (Eppendorf ThermoMixer® C) shaking at 1000 rpm ...
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bioRxiv - Microbiology 2022Quote: ... Two-microliter aliquots of resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
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bioRxiv - Microbiology 2022Quote: ... Two μl aliquots of the resuspended lipids were diluted 1:10 in 10 mM ammonium acetate in methanol in 96-well plates (Eppendorf twin tec 96) prior to measurement ...
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bioRxiv - Bioengineering 2024Quote: ... 40 µL of clarified lysate was aliquoted into full-skirted PCR plates and incubated at the reported temperature for 1 h using a gradient on a Mastercycler® X50s (Eppendorf, Catalog # 6311000010). Room temperature controls were incubated for 1 h on the benchtop in 200-µL PCR tubes ...