Labshake search
Citations for Omega Bio-Tek :
201 - 250 of 755 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... RNA was extracted with E.Z.N.A.® Soil RNA Kit (Omega bio-tek, USA). The cDNA library of each sample was constructed and sequenced on the HiSeq 3000 platform (Illumina ...
-
bioRxiv - Genomics 2023Quote: ... DNA was subsequently purified by a 2X SPRI (Omega Bio-Tek, M1378-01). Sequencing libraries were then prepared from 5 ng of DNA by performing end-repair with the End-it DNA End-Repair Kit (Lucigen ...
-
bioRxiv - Plant Biology 2023Quote: ... Total tRNA was extracted using a microRNA kit (Omega Bio-Tek, Norcross, USA). Five micrograms of tRNA were hydrolyzed in 10 μL enzymic buffer (1 U Benzonase ...
-
bioRxiv - Genomics 2023Quote: ... DNA was amplified by PCR with Phusion HF 2X MM and Nextera universal barcodes described previously and was purified by two-sided SPRI purification (Omega Bio-Tek, M1378-01). Samples were sequenced using a NovaSeq SP kit (Illumina ...
-
bioRxiv - Microbiology 2023Quote: Total genomic DNA was extracted from all the collected samples using the E.Z.N.A.® Soil DNA kit (Omega Bio-tek, Norcross, GA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... the samples were diluted 500 times and filtered using the DNA Mini columns with a nucleic acid-affinity membrane (Omega Bio-tek.) to decrease or remove the nucleic acid in samples ...
-
bioRxiv - Synthetic Biology 2023Quote: ... gDNA was extracted from each clonal population (Omega Bio-Tek) and 50 ng of gDNA was used as the template in a 25 µL reaction along with 0.25 µL of Q5 polymerase (NEB) ...
-
bioRxiv - Genomics 2023Quote: ... we have improved the results by purifying the extracted DNA using Omega Mag-Bind TotalPure NGS Beads (Omega Bio-Tek, Norcross, GA), which helps to preserve the life span of the flow cell.
-
bioRxiv - Cancer Biology 2023Quote: ... using E.Z.N.A.® Plasmid Mini Kit I (Omega Bio-tek, Inc.). To produce lentiviral media ...
-
bioRxiv - Cell Biology 2023Quote: DNA was extracted from cell pellets using an EZNA Tissue DNA Kit (Omega Bio-Tek). Extracted DNA samples were subjected to quantitative polymerase chain reaction (qPCR ...
-
bioRxiv - Genetics 2023Quote: ... and plasmids were isolated from the recovered cell populations using a commercial kit (OMEGA Bio-Tek E.Z.N.A. Yeast Miniprep kit) as per manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... The PCR products were purified (Omega E.Z.N.A Cycle Pure kit, Omega Bio-tek Inc., Norcross GA), digested with BsrGI and AflII ...
-
bioRxiv - Genetics 2023Quote: ... The PCR products were cleaned (OMEGA Bio-Tek E.Z.N.A. Cycle Pure Kit), quantified (Synergy H1 Hybrid Multi-Mode Microplate Reader) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and recombinant bacmids were purified using the E.Z.N.A.® Plasmid Mini Kit I (Omega Bio-tek, cat. no. D6942-01). Baculoviruses were produced by transfecting Sf9 cells with recombinant bacmids using TransIT®-Insect Transfection Reagent (Mirus ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... was produced in DH10B cells and the plasmid purified from single-bacterial colonies using the E.Z.N.A.® Plasmid Mini Kit I (Omega Bio-tek, cat. no. D6942-01). NLFK cells were transfected using Lipofectamine 2000 (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... DNA was isolated using the E.Z.N.A.® plasmid DNA minikit (Omega bio-tek, D6942-02), and 1 µg of isolated DNA was loaded and run on a 0.45% agarose gel in TBE (26 V at 4 °C for 18-20 h) ...
-
bioRxiv - Microbiology 2023Quote: Plasmid DNA was routinely extracted using the EZNA Plasmid DNA Mini Kit (Omega Bio-tek) and eluted in 10 mM Tris-HCl pH 8.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by purification and size-selection by magnetic beads (Mag-Bind Total Pure NGS, Omega Bio-Tek) using a 0.56x bead ratio ...
-
bioRxiv - Biochemistry 2023Quote: ... dsDNA was purified and size selected using magnetic beads (Mag-Bind Total Pure NGS, Omega Bio-Tek) using a 0.65x bead ratio ...
-
bioRxiv - Biochemistry 2023Quote: ... RT reactions were purified using magnetic beads (Mag-Bind Total Pure NGS, Omega Bio-Tek). 2A3-probed RMRP and RNase P samples were reverse transcribed using the SSII protocol.
-
bioRxiv - Genomics 2023Quote: ... Bacterial DNA Kit (Omega Bio-Tek, Norcross, GA, USA) followed DNA sequencing on an Illumina Hiseq 2500 PE150 platform (Illumina ...
-
bioRxiv - Biochemistry 2023Quote: ... PCR1 products were purified (Mag-Bind Total Pure NGS, Omega Bio-Tek) using a 0.7x bead ratio ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... We dissected the megagametophytes from the germinating seeds and extracted DNA with E.Z.N.A Plant DNA DS Kit (Omega BIO-TEK). We quantified the DNA concentration with NanoDrop ND-1000 (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2023Quote: 45-50 inflorescence meristems for each of three biological replicates of Col-0 and clv3-9 were collected and total RNA isolated using the EZNA Plant RNA kit (Omega Bio-tek). RNA was treated with RNase-free DNase (Omega Bio-tek ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... DNA of 80 specimens was extracted from gill tissue snips with the E.Z.N.A Mollusc DNA Kit (Omega Bio-tek, Norcross, GA) at the University of Rhode Island and sent for plexWell384 library preparation and NovaSeq6000 2x150 bp sequencing at Psomagen ...
-
bioRxiv - Microbiology 2023Quote: ... dissected body parts or tissues were homogenized in either 200 µl DMEM medium for end-point titration on BHK-21 cells or in 1 mL RNA-Solv reagent (Omega Bio-Tek) for RNA isolation ...
-
bioRxiv - Microbiology 2023Quote: Aag2 cells were homogenized in RNA-Solv reagent (Omega Bio-Tek) and RNA extraction was performed according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The ligations were then purified with a MicroElute kit (OMEGA Bio-tek Inc.) and eluted with 20 μL of water.
-
bioRxiv - Genomics 2023Quote: ... Mag-Bind Blood DNA Kit (Omega Bio-Tek, Inc., Norcross, GA) or ArchivePure DNA Blood Kit (5 Prime ...
-
bioRxiv - Plant Biology 2023Quote: ... and DNase treated with EZNA RNase-Free DNase I (Omega Bio-tek). RNA concentration and purity were verified using a NanoDrop Spectrophotometer ND-1000 (Thermo Scientific) ...
-
bioRxiv - Plant Biology 2023Quote: ... Plant RNA Kit (Omega Bio-tek) and DNase treated with EZNA RNase-Free DNase I (Omega Bio-tek) ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA extraction from tissues or intestinal epithelial fraction was perform using EZNA® Total RNA kit (Omega Bio-tek) with bead column for more efficiency cell lyses ...
-
bioRxiv - Microbiology 2023Quote: ... RNA was extracted using RNA isolation kit (Omega Bio-tek). SARS-CoV-2 RNA levels were measured by one-step quantitative reverse transcriptase PCR (RT-qPCR ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the E.Z.N.A.® Cycle Pure kit (Omega Bio-Tek, Inc, GA, USA), product concentrations were measured using the NanoDrop™ 2000 Spectrophotometer (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... aureus RN4220 using the E.Z.N.A.® Plasmid DNA Mini Kit I (Omega Bio-Tek, Inc, GA, USA), and purified plasmids pSepiCcrAB ...
-
bioRxiv - Microbiology 2023Quote: ... SP Plant DNA Kit (Omega Bio-Tek Inc., Norcross, Georgia) and eluted in 100 µL sterile TE buffer ...
-
bioRxiv - Genomics 2023Quote: ... Total RNA Kit (Omega Bio-Tek). RNA concentration was measured using a Nanodrop 2000 (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2023Quote: ... DNA was isolated from leaves and seeds using the E-Z 384 Plant DNA HT kit (Omega Bio-tek, GA, USA).
-
bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted from 500 μl of tissue slurry using the E.Z.N.A.® DNA/RNA Isolation Kit (Omega Bio-Tek) and then stored at −80°C until further processing.
-
bioRxiv - Microbiology 2023Quote: ... We purified amplicons using the Mag-Bind E-Z Pure Kit (Omega Bio-tek, cat # M1380) and quantified with Invitrogen Quant-iT PicoGreen dsDNA Reagent ...
-
bioRxiv - Plant Biology 2023Quote: ... RNA was treated with RNase-free DNase (Omega Bio-tek) and 1.5 ug RNA was used for library preparation at the High-throughput Sequencing Facility at UNC Chapel Hill using the Stranded mRNA-Seq kit (Kapa Biosystems ...
-
bioRxiv - Cell Biology 2023Quote: ... DNA was purified (Gel Extraction Kit; Omega Bio-Tek, Norcross, GA) and validated via Sanger sequencing.
-
bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from 100 mg of grounded leaf tissue using the E.Z.N.A Plant Mini Kit (Omega Bio-tek, Norcross, USA) and contaminating DNA was removed using turbo DNAfree DNAse I (Ambion ...
-
bioRxiv - Plant Biology 2023Quote: ... Disk samples around infection site were taken and DNA extraction was performed using an E.Z.N.A.® Stool DNA Kit (Omega Bio-Tek, Norcross, USA). For the quantification of DNAs standard curves were prepared using primers designed as follows ...
-
bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted from T0/T1 plants’ leaves using E.Z.N.A.® Plant DNA Kit (Omega Bio-Tek, Norcross, USA). The screening for hCas9 presence was performed using primers reported in Data S1 by PCR using KAPA HIFI Taq (Kapa Biosystems ...
-
bioRxiv - Genomics 2023Quote: ... DNA was isolated according to the tissue protocol of the Mag-Bind® Blood & Tissue DNA 96 Kit (#M6399-01, Omega Bio-tek) and quantified using the Quantifluor® dsDNA System (#E2670 ...
-
bioRxiv - Biochemistry 2023Quote: Barcoded NGS libraries for each sorted population were generated using a two-step PCR protocol using AmpliTaq Gold (Invitrogen. The resulting PCR products were purified using Mag-Bind® TotalPure NGS beads (Omega Bio-tek) and amplified in a second PCR introducing the standard Illumina adapters ...
-
bioRxiv - Microbiology 2023Quote: ... frugiperda was extracted using the OMEGA Tissue DNA Kit (Omega Bio-Tek, Norcross, GA, USA), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... genomic DNA was sheared to 400 bp using the Covaris LE220 instrument and size selected with SPRI using TotalPure NGS beads (Omega Bio-tek). Library prep included fragment end treatment ...
-
bioRxiv - Microbiology 2023Quote: The rust layer biofilm and seawater samples were subjected to DNA isolation using the Bacterial DNA Isolation Kit (Mobio, Carlsbad, CA, USA) and Water DNA Isolation Kit (Omega Bio-tek, Doraville, GA, USA), respectively ...