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Citations for Thermo Fisher :
1 - 50 of 58 citations for trans Cotinine amide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Starting resin used was Fmoc-Amide Resin (Applied Biosystems). Cleavage from resin and simultaneous amino acid side chain deprotection was accomplished using ...
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bioRxiv - Immunology 2022Quote: ... Starting resin used was Fmoc-Amide Resin (Applied Biosystems). Cleavage from resin and simultaneous amino acid side chain deprotection was accomplished using ...
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bioRxiv - Microbiology 2024Quote: ... Starting resin used was Fmoc-Amide Resin (Applied Biosystems). Cleavage from resin and simultaneous amino acid side chain deprotection was accomplished using ...
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bioRxiv - Microbiology 2024Quote: ... and Accucore™ 150-Amide-HILIC (4.6 × 100 mm, 2.6 μm, Thermo Scientific) were used in the separation of metabolites in positive and negative modes ...
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bioRxiv - Molecular Biology 2021Quote: Human NEMO capped peptide (residues 226-235, acetyl-KLAQLQVAYH-amide, synthesized by Thermo Scientific) was dissolved to 2 mM in peptide buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... HEPES pH 7 was added (25 mM final) and semi-soluble IANBD Amide (Invitrogen) from a stock (41 mM ...
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bioRxiv - Biochemistry 2020Quote: ... XBridge Amide column (Waters) was used for SAM detection and ODS Hypersil C18 column (Thermo Scientific) for 5′-dA ...
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bioRxiv - Neuroscience 2022Quote: LC was performed using a Xbridge BEH amide HILIC column (Waters) with a Vanquish UHPLC system (Thermo Fisher). Solvent A was 95:5 water ...
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bioRxiv - Biochemistry 2023Quote: The oligonucleotides were separated using an Xbridge BEH Amide column (2.1 x 150mm, 2.5um, Waters) with an Ultimate 3000 UHPLC (Thermo Scientific) coupled to an LTQ-XL mass spectrometer (Thermo Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... HILIC separation was employed with a Waters ACQUITY UPLC BEH amide column (2.1 ×150 mm, 1.7 μm particle size) on a Vanquish (ThermoFisher Scientific) chromatograph ...
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DeFrND: detergent-free reconstitution into native nanodiscs with designer membrane scaffold peptidesbioRxiv - Biophysics 2024Quote: ... N,N’-Dimethyl-N-(Iodoacetyl)-N’-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Ethylenediamine (IANBD amide) and Oregon GreenTM 488 (OG) maleimide were obtained from ThermoFisher. All other chemicals were acquired from Sigma.
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bioRxiv - Plant Biology 2021Quote: ... Plant samples were separated using an Accucore-150-Amide-HILIC column (150 × 2.1 mm; particle size 2.6μM; Thermo Scientific 16726-152130) with a guard column containing the same column matrix (Thermo Scientific 852-00 ...
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bioRxiv - Biochemistry 2021Quote: ... with a 10-fold excess of N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylenediamine (IANBD-amide, Molecular Probes). After 90 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... The analytical gradients were carried out using an Accucore 150-Amide-HILIC column (2.6 μm, 2.1 mm × 100 mm, Thermo Fisher Scientific) with solvent A (5 mM ammonium acetate in 5% acetonitrile ...
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bioRxiv - Cancer Biology 2023Quote: ... with a UPLC BEH Amide column (2.1 mm × 100 mm, 1.7 μm) coupled to Q Exactive HFX mass spectrometer (Orbitrap MS, Thermo Fisher Scientific). The mobile phase consisted of 25 mmol L−1 ammonium acetate and 25 ammonia hydroxide in water (pH = 9.75 ...
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bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
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bioRxiv - Bioengineering 2020Quote: ... Serial dilutions of trans-4-hydroxy-Lproline (Fluka, ThermoFisher) in papain solution were used to obtain a standard range ...
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bioRxiv - Cell Biology 2023Quote: ... then trans-cardially perfused with DPBS (Gibco, 14190–094) to remove blood ...
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bioRxiv - Immunology 2024Quote: Polar extracts were analysed on a Accucore-150-Amide-HILIC column (100 x 2.1 mm, 2.6 μm, Thermo Fisher Scientific, MA, USA). For positive ion mode analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... was annealed from two components: trans-activating crRNA (A35507, Invitrogen) and crRNA targeting specific gene (A35509 ...
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bioRxiv - Neuroscience 2023Quote: ... and 10 μM all-trans retinoic acid (RA; ThermoFisher Scientific) for 48 hours ...
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bioRxiv - Neuroscience 2023Quote: One μL of reconstituted standards was injected into a Thermo Fisher Scientific™ Accucore™ 150 Amide HILIC (150 × 3 mm, 2.6 mm particle size; Thermo Fisher Scientific). Chromatographic separation was achieved using the following conditions ...
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bioRxiv - Cell Biology 2022Quote: ... at a flow rate of 200 μL/min on an Accucore™-150-Amide-HILIC column 2.6 μm (100 × 2.1 mm) (Thermo Fisher Scientific, Cat 16726-102130) at 40 °C at a gradient from 45% to 90% A in 15 minutes followed by a 10-minute isocratic step ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Transfections were performed with Trans-IT 293T transfection reagent (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and 10 μM of freshly diluted all-trans retinoic acid (Fisher Scientific). The medium was refreshed at least once during the differentiation period ...
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bioRxiv - Cell Biology 2023Quote: Cultured cells were treated with all-trans retinoic acid (RA, 1 µM, Fisher Scientific), high affinity pan-RAR antagonist AGN-193109 (AGN ...
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bioRxiv - Genetics 2024Quote: ... the medium was additionally supplemented with 1 µM all-trans retinoic acid (Thermo Fisher). To differentiate towards EpiLC fate ...
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bioRxiv - Cancer Biology 2022Quote: ... EZ Trans transfection reagent (LIFE iLAB BIO, China) was mixed DNA mix and OPTI-MEM (Gibco) and incubated for 20 ∼ 40 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... and trans-cardially perfused with phosphate-buffered saline (PBS) followed by 10% neutral buffered formalin (Fisher Scientific). Brains were extracted ...
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bioRxiv - Cell Biology 2022Quote: All the above constructs were transfected into Phoenix GP cells using trans-lentiviral packaging system (ThermoFisher Scientific). The virus was collected ...
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bioRxiv - Cell Biology 2024Quote: ... The retina:RPE explant was transferred to a trans well insert with 0.4-micron pore polycarbonate membrane (ThermoFisher) sitting on the surface of 1.5 mL of neurobasal-A plus medium (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... albicans mono culture was added to the inside of the 0.4 µm trans well inserts (Thermo Scientific, 140660) whereas 1.5 mL of S ...
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bioRxiv - Immunology 2023Quote: ... Samples requiring intracellular staining were subsequently incubated in FoxP3 Tran-scription Factor Fixation/Permeabilization Concentrate and Diluent (Life Technologies) for 30 minutes at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Whole synovium from a single knee was placed into a seated 12-well trans-well insert (Fisher Scientific, 353494) with serum free 1:1 DMEM/F12 with 1% penicillin-streptomycin in both chambers and equilibrated for 24 hours before transfer into co-culture ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the BioRad Trans-Blot Transfer using the 5-minute protocol and blocked in StartingBlock (Thermo Fisher, 37543) TBS blocking buffer for 30 min at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... Promoter entry clones were linked to entry clones containing their respective HA-PpCSLD coding sequences and inserted into pSi3(TH)GW (Tran and Roberts 2016b) using LR Clonase II Plus (Invitrogen). These vectors target the expression cassettes to the intergenic 108 locus ...
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bioRxiv - Cancer Biology 2021Quote: ... each LeGo plasmid was transfected into HEK293T cells together with the Trans-Lentiviral shRNA Packaging System (#TLP5912; Dharmacon) helped by Lipofectamine 2000 (#11668030; Invitrogen). Forty-eight hours post-transfection viral particles in the supernatant were collected ...
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bioRxiv - Neuroscience 2022Quote: Mice were injected with Pentobarbital (250 mg/kg) and tran-scardially perfused with pre-chilled phosphate buffered saline (PBS, Gibco) followed by pre-chilled 4% paraformaldehyde (PFA ...
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bioRxiv - Cell Biology 2024Quote: Stable arpin-depleted HUVEC were generated by lentiviral transduction using the trans-lentiviral packaging kit (Thermo Fisher Scientific, Waltham, MA) and the pLKO.1 plasmid (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... were transfected with SMARTPool siRNA (25-50 nM, Horizon) via Trans-IT X2 (Mirius Bio, MIR6004) in Opti-MEM (Gibco). SMARTPool siRNA catalog numbers are listed in Table 2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Recombinant expression of the pFastBac-BRIL-MOR construct bound to either cis or trans AM- 3 was performed in Spodoptera frugiperda (Sf9) insect cells using the Bac-to-Bac expression system (Gibco). Briefly ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was prepared using M-MLV Reverse Transcriptase and qRT-PCR were conducted using the SYBR Premix Ex Taq kit (Trans) on a Step One System (Applied Biosystems). The quantification method (2−ΔCt ...
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bioRxiv - Cancer Biology 2021Quote: A lentiviral pLKO.1-puro constructs targeting KSR1 and non-targeting control were transfected into HEK-293T cells using trans-lentiviral packaging system (ThermoFisher Scientific). The virus was collected ...
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bioRxiv - Immunology 2021Quote: ... RRV cDNA was generated from 10 μl of serum derived RNA or 1 μg of tissue derived RNA using a sequence-tagged (indicated with lower case letters) RRV-specific RT primer (5′-ggcagtatcgtgaattcgatgcAACACTCCCGTCGACAACAGA-3′) with SuperScript IV reverse tran-scriptase (Life Technologies). RRV genomes were quantified by RT-qPCR using a tag sequence-specific reverse primer (5′-GGCAGTATCGTGAATTCGATGC-3′ ...
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bioRxiv - Cancer Biology 2024Quote: ... and MSCV KSR2 FIF570 IRES YFP resistant to binding hairpin sh5 (FIF570r) were transfected into HEK-293T cells using trans-lentiviral packing system (ThermoFisher Scientific). The virus was collected 48 hours post transfection and used to infect KP1 sh5 cells with 8 µg/mL Polybrene for 72 hours ...
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bioRxiv - Cell Biology 2020Quote: Mice were terminally anesthetised with an intraperitoneal injection of pentobarbital sodium (Euthatal, Merial) then trans-cardially perfused with DPBS (Gibco, 14190-094) to remove blood ...
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bioRxiv - Cancer Biology 2020Quote: Cells were transfected by adding 1 ml serum-free culture media with Trans-IT X2 transfection reagent (MirusBio) and targeting (On-Target Smartpool siRNA) or scrambled (siCTRL) siRNA (Dharmacon, Thermo Fisher Scientific) twice on two consecutive days ...
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bioRxiv - Microbiology 2023Quote: ... and pC-L (1 μg) that supply the trans-acting factors LCMV NP and L 70 using 10 μL of Lipofectamine 2000 (Thermo Fisher Scientific) and incubated at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was produced in HEK-293T cells using either the Trans-Lentiviral ORF Packaging Kit (Dharmacon/Horizon Discovery) or Lipofectamine 3000 (Thermo Fisher Scientific). Viral supernatants were collected 72 hours (hrs ...
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bioRxiv - Neuroscience 2024Quote: ... mitotic SH-SY5Y cells were differentiated into non-mitotic neuronal cells by the addition of 20 µM all-trans retinoic acid on day 1 (Molecular Probes/Invitrogen, #207340010). From day 1 onwards ...