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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Q-RT-PCR was performed using TaqMan primers/probe sets (Applied Biosystems) and analyzed as transcript copies per 50 ng RNA expressed relative to RNAPII expression as previously described (33).
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using Taqman probes and primer sets (Applied Biosystems) specific for CD36 (assay ID Mm00432398_m1) ...
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bioRxiv - Neuroscience 2024Quote: ... Primers used were: rno-miR-155-5p (RT:002571, ThermoFisher Scientific), and rno-miR-674-3p (RT:001956 ...
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bioRxiv - Physiology 2020Quote: ... The multiplex RT primer pool consisted of primers for miR-150-5p and U6 (Thermo Fisher Scientific). No-template samples where included as negative controls.
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bioRxiv - Microbiology 2020Quote: ... 18S rRNA endogenous control primer/probe set was utilized for semi-quantitative RT-PCR normalization (ThermoFisher, 4333760T). Thermal cycling conditions were adapted from Verso 1-step RT-qPCR kit (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 U Ribolock (40U/μl), 1x TaqMan RT primer per each gene of interest (miR-7a, miR-671, Let-7a, snoRNA202, U6 snRNA) (Applied Biosystems) and 1x first-strand synthesis buffer were filled up to 20 μl reaction volume with ddH2O and mixed ...
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bioRxiv - Pathology 2021Quote: ... PCR primers and probe sets were obtained from Thermofisher, USA ...
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bioRxiv - Cancer Biology 2021Quote: ... Two sets of primers were used: Megaplex RT Primers Human pool A and B (Thermo Fisher, USA). Together ...
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bioRxiv - Cancer Biology 2019Quote: 50-100ng of genomic DNA from edited cells in a total PCR reaction volume of 50ul were amplified in a 30 cycle PCR using a locus-specific primer set (primer set 1) and AmpliTaq Gold 360 Master Mix (Applied Biosystems) (Supplemental Table 1b) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1-2μl of extracted genomic DNA in a total PCR reaction volume of 25μl were amplified in a 35 cycle PCR using a locus-specific primer set (primer set 1) and AmpliTaq Gold 360 Master Mix (Applied Biosystems) (Supplemental Table 1b) ...
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bioRxiv - Cancer Biology 2021Quote: ... with miR-92a-specific primers (Applied Biosystems). Quantitative PCR was performed using FAST SYBR green mix (Roche ...
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bioRxiv - Plant Biology 2022Quote: Custom TaqMan® probes (including RT primers and qRT-PCR primers, Thermo Scientific) were designed using the Small RNA Assay Design Tool with the following input sequences ...
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bioRxiv - Cancer Biology 2019Quote: ... Knockout in tissue or cells post-cre was confirmed by Q-RT-PCR using primer/probe sets for CIC (Applied Biosystems) and by Western blotting with anti-Cic.
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bioRxiv - Immunology 2023Quote: ... Real-time RT-PCR was performed using 50 ng of RNA and the Platinum Quantitative RT-PCR ThermoScript One-Step System with FAM primers and probe sets from Applied Biosystems (CCL2 ...
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bioRxiv - Microbiology 2020Quote: ... Genomic RNA was then amplified in six equal-sized segments using overlapping primers sets (Supplementary Table S8) using SuperScript III One-Step RT-PCR kit (ThermoFisher Scientific, USA) with Platinum Taq ...
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bioRxiv - Microbiology 2024Quote: ... extracted RNA was mixed with either Uni or Opti primer sets in a Platinum SuperScript III One Step RT-PCR kits (Invitrogen, Carlsbad, CA) for amplification ...
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bioRxiv - Cancer Biology 2019Quote: ... precursor and mature miR-15a/miR-16 specific primers were purchased from Thermo Fisher. cDNA synthesis was performed by the High Capacity cDNA Synthesis Kit (Thermo Fisher ...
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bioRxiv - Bioengineering 2023Quote: ... and primers specific for mmu-miR-147-3p (alias, hsa-miR-147b; Applied Biosystems). The relative abundance of EV miRNA was determined by TaqMan PCR using TaqMan Universal PCR Master Mix II and TaqMan primers and probes from Applied Biosystems ...
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR reactions were then set up using RT-PCR kit (Applied Biosystems, ThermoFisher Scientific). Primers used are listed in Table S3.
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR reactions were then set up using RT-PCR kit (Applied Biosystems, ThermoFisher Scientific). Primers used are listed in Table S3.
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bioRxiv - Cell Biology 2020Quote: ... PCRs with different primer sets were conducted by using DreamTaq Green PCR Master Mix (Thermo Scientific) or PrimeSTAR DNA Polymerase kit (Takara) ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... RT-qPCR amplification was carried out using ready-to-use Taqman probe/primer sets (Applied Biosystems) to detect expression levels for Lyve1 (Mm00475056_m1) ...
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bioRxiv - Immunology 2021Quote: ... using TaqMan PCR Master Mix and premixed primers/probe sets (Thermo Fisher Scientific) sets specific for Itgav (Mm00434486_m1) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... KDM primer/probe sets used for qRT-PCR were from Thermo Fisher (4392420): Hs00231908_m1 ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was set up using the SYBR Green PCR Master Mix (ThermoFisher) and conducted using the StepOnePlus RT-PCR machine (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... and rno-miR-674-3p (RT:001956, ThermoFisher Scientific). Data were analyzed using StepOne Software v ...
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bioRxiv - Cell Biology 2023Quote: ... followed by equilibration in 5mM MgCl2/PBS at RT and incubation in 1X SSC [20X SSC (Invitrogen AM9763) diluted in Nuclease free water (AmericanBio ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR probe and primer sets (Table S2) were designed using Primer Express version 3.0 (Life Technologies, Carlsbad, CA). qRT-PCR assay was performed using the AgPath-ID One-Step RT-PCR Buffer and Enzyme Mix (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... cDNA was synthesized by RT-PCR using oligo d(T) primer (Invitrogen) on a T100 thermal cycler (Bio-Rad) ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analyses were conducted utilizing the Centers for Disease Control 2019-nCoV N1 primers and probe (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html) and AgPath-ID One-Step RT-PCR reagents (Ambion/ThermoFisher, Waltham, Massachusetts). We included a standard curve of serial dilutions of RNA extracted from SARS-CoV-2 virus stock (107 TCID50/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... MERS-CoV and REBOV were diluted to 2×10-3 and confirmed by their respective monoplex one-step RT-PCR with specific primers using the Verso 1-step RT-PCR kit (Thermo Scientific). The reaction was set up in 10 μl final volume with 100 nM each of unlabelled forward and reverse primers ...
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bioRxiv - Molecular Biology 2021Quote: ... Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific web site ...
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bioRxiv - Neuroscience 2019Quote: ... and TaqMan primer probe sets (ThermoFisher). TaqMan assay ID numbers used ...
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bioRxiv - Microbiology 2020Quote: ... GAPDH primer/probe sets (Thermo Fisher) were used for normalization of cellular RNA levels.
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bioRxiv - Biochemistry 2021Quote: ... The sets of primers (ThermoFisher Scientific) were as follows ...
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bioRxiv - Immunology 2020Quote: ... Taqman primer/probe sets (Thermo Fisher) were as follows ...
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bioRxiv - Neuroscience 2023Quote: ... Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific website (Supplementary Table 3) ...
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bioRxiv - Cell Biology 2022Quote: ... for miR-375 was quantified by TaqMan Assays using the TaqMan MicroRNA Reverse Transcription Kit and hsa-miR-375 primer sets (Thermo Fisher Scientific, 000564). MiR-375 levels were normalized to miR-U6 expression ...
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bioRxiv - Immunology 2019Quote: ... and different primer sets using a QuantStudio 5 Real-Time PCR System (Applied Biosystems) thermocycler ...
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bioRxiv - Microbiology 2023Quote: ... with appropriate primer sets on a QuantStudio 5 Real-Time PCR System (Applied Biosystems). Primers for qRT-PCR analysis of Alrs ...
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bioRxiv - Immunology 2019Quote: ... Taqman miR primers and fluorescent probes were from Life Technologies. Quantitative PCR conditions for the Stratagene Cycler were ...
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bioRxiv - Neuroscience 2024Quote: ... we used rno-miR-191a-3p (RT:002576, ThermoFisher Scientific), which was selected as the most stable in our experiment ...
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bioRxiv - Pathology 2023Quote: ... TaqMan MGB primer/probe sets and Eukaryotic 18S rRNA VIC primer/probe set endogenous control (Thermo Fisher) with Premix Ex Taq master mix (Takara ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analyses were conducted utilizing the Centers for Disease Control 2019-nCoV N1 primers and probe (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html) and AgPath-ID One-Step RT-PCR reagents (Ambion/ThermoFisher, Waltham, Massachusetts). We included a standard curve of serial dilutions of RNA extracted from SARS-CoV-2 virus stock (107 TCID50/ml ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR assays for miR-US5-1 and miR-UL112 were purchased from ThermoFisher Scientific.
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bioRxiv - Microbiology 2021Quote: ... 5µl were subjected to quantitative PCR using primer/probe sets for human Gapdh (Applied Biosystems), and EBOV trVLP 5’-trailer region ...
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bioRxiv - Immunology 2022Quote: ... and primer sets (Supplementary Table 1) on a StepOnePlus Real-Time PCR System (Applied Biosystems).
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bioRxiv - Developmental Biology 2019Quote: All primers were Assay-on-Demand primer/probe sets from ThermoFisher Scientific (Richlands ...
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bioRxiv - Cancer Biology 2020Quote: ... Primers for miR-147b and U6 were purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and target-specific primer/probe sets (ThermoFisher) or frozen at −80° C until use ...