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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... on a StepOne Plus RT-PCR System (Applied Biosystems).
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bioRxiv - Physiology 2021Quote: ... TaqMan real-time RT-PCR assay (Thermo Fisher Scientific): mRNA expression of SMA (Hs00426835_g1) ...
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bioRxiv - Physiology 2020Quote: ... using the SuperScript III RT-PCR kit (Life Technologies), employing reverse transcriptase in the presence of deoxynucleotides (dNTP ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR Step One Plus system (#8024, Applied Biosystems) was used.
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bioRxiv - Cell Biology 2021Quote: ... and QuantStudio 3 RT-PCR system (Thermo Fisher Scientific). All expression levels were normalized to that of RPS18 mRNA ...
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bioRxiv - Genetics 2022Quote: ... RT-PCR was performed using PowerUP Sybr (ThermoFisher, #A25741) based on manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative RT-PCR was performed using StepOnePlus (Applied Biosystems). Relative expression was calculated using the ΔΔCt method with the reference gene ef1α ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were run on AgPath RT-PCR (Life Technologies) kit on a QuantStudio™ 7 Flex Real-Time PCR System and the relative amounts of FAM and HEX signal used to determine the proportion of Omicron and Delta in the original samples ...
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bioRxiv - Bioengineering 2020Quote: ... with an ABI 7500 RT-PCR System (Applied Biosystems). Data were analyzed with 7500 Software using relative quantification analysis.
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bioRxiv - Genetics 2020Quote: ... RT-PCR was performed using SuperScriptIII Reverse Transcriptase (ThermoFisher) with random primers ...
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bioRxiv - Cancer Biology 2021Quote: ... and RT-PCR Grade Water (Invitrogen, Carlsbad, CA, USA). Using a C100 thermal cycler (BioRad) ...
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bioRxiv - Developmental Biology 2022Quote: ... in a StepOne Plus RT-PCR apparatus (Applied Biosystems). Gene expression was assessed by the comparative CT (ΔΔCt ...
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bioRxiv - Molecular Biology 2022Quote: ... Using Verso 1-Step RT-PCR kit (Thermo Scientific), reactions of 13 μl final volume was set up using 0.6 μM of triplex primer cocktail in equimolar ratio of labelled forward and reverse primers of each target ...
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bioRxiv - Plant Biology 2023Quote: ... on a QuantStudio 5 RT-PCR system (Applied Biosystems). Sequencing was performed at the University of California Davis Genome Center using a HiSeq 4000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... and conducted using the StepOnePlus RT-PCR machine (ThermoFisher). All data was normalized to endogenous controls GAPDH (human ...
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bioRxiv - Biochemistry 2023Quote: ... A QuantStudio 3 RT-PCR machine (Thermo Fisher Scientific) was used to measure melting temperatures.
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bioRxiv - Pathology 2023Quote: ... cDNA was amplified with RT-PCR master mix (ThermoFisher) and ran in Bio-Rad PCR Thermal Cycler ...
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bioRxiv - Cancer Biology 2023Quote: ... and Quantstudio 7 flex RT-PCR system (ThermoFisher Scientific). Expression was measured and the ΔCT was calculated ...
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bioRxiv - Neuroscience 2023Quote: ... in a QuantStudio3 RT-PCR system (Applied Biosystems™) based on manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-PCR was conducted using validated TaqMan assays (ThermoFisher, Hs00271949_m1 for TBX1 ...
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bioRxiv - Developmental Biology 2023Quote: ... on a 7300 fast RT-PCR System (Applied Biosystems) with pre-designed exon-spanning reaction mixes (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Total expression levels of miR-675-3p and miR-675-5p were determined relative to snoRNA202 by using a separate RT kit (TaqMan MicroRNA Reverse Transcription Kit, Thermo Fisher Scientific), qRT-PCR primers (Assay Id 001232 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the reverse-transcriptase reaction using RT primers and SuperScriptIII (Thermo Fisher Scientific). cDNA libraries were amplified by means of Phusion High-Fidelity DNA Polymerase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... before reverse transcription with Superscript III RT using random primers (200 ng/reaction, Invitrogen). Quantitative PCR reactions were carried out with the indicated primer pairs (Eurofins MWG Operon ...
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bioRxiv - Molecular Biology 2020Quote: ... before reverse transcription with Superscript III RT using random primers (200 ng/reaction, Invitrogen). Quantitative PCR reactions were carried out with the indicated exonic primer pairs (Eurofins MWG Operon ...
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bioRxiv - Physiology 2020Quote: ... and custom Multiplex RT Primer pool in a SimpliAmp™ Thermal Cycler (Applied Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... reverse transcription was performed using oNTI223 primer and superscript III RT kit (Life Technologies). The cDNA products were separated on a 10% polyacrylamide TBE-urea gel and only those fragments migrating between 100-500 bp were excised and recovered by gel extraction ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs were synthesized using random primers and Moloney murine leukemia virus RT (Invitrogen). The RT-PCR products were separated on a 2% w/v agarose-TBE gel ...
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bioRxiv - Cell Biology 2022Quote: ... TaqMan RT primers against human occludin (Hs05465837_g1) and GAPDH (Hs02786624_g1) were purchased from Invitrogen.
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bioRxiv - Microbiology 2021Quote: ... These primers were used for cDNA synthesis (Thermo Scientific RevertAid RT Reverse Transcription Kit) according to manufacturer’s instruction with an annealing step at 16°C instead of 25°C and were used in multiplex to target respective fungal sRNAs and barley miRNAs Hvu-mir159 and Hvu-mir168 as references ...
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bioRxiv - Microbiology 2022Quote: ... RNA was converted into cDNA using random hexamer primers and Revert aid RT (Invitrogen). Selected candidates were then analyzed by RT-qPCR using specific primers ...
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bioRxiv - Microbiology 2019Quote: ... total RNA was reverse transcribed using virus-specific RT primers or oligo-dT primers and the SuperScript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA). Real-time quantitative PCR was performed on the Applied Biosystems 7500 system with QuantiTect SYBR Green PCR Master Mix (Qiagen ...
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bioRxiv - Bioengineering 2022Quote: ... or a negative control miR (Invitrogen mirVana miRNA Mimic ...
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bioRxiv - Molecular Biology 2020Quote: ... and miR-183-5p (Applied Biosystems). Reverse transcription products were then amplified by quantitative PCR (95 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Reaction set-up included 2.5ul of 10X PCR Buffer (Thermo Scientific, USA), 1.5 mM MgCl2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Assay identifiers are listed for TaqMan primer/probe sets purchased from Thermo Fisher (or IDT where indicated).
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bioRxiv - Plant Biology 2022Quote: A genomic fragment from 4.3 kb upstream of the transcriptional start site to the last codon of MpHYP was amplified using the primer set MpHEC1_pro_F and MpHEC1_CDS_nsR and cloned into the pENTR/D-TOPO vector (Thermo Fisher), followed by LR reaction with pMpGWB301 (Ishizaki et al. ...
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bioRxiv - Plant Biology 2022Quote: The coding sequence of MpHYP without the stop codon was amplified from Tak-1 cDNA using the primer set MpHEC1_CDS_F and MpHEC1_CDS_nsR and cloned into pENTR/D-TOPO (Thermo Fisher), followed by an LR reaction with pMpGWB313 (Ishizaki et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Exogenous mRNA levels of transgene expression human APOE (Hs00171168_m1) commercial TaqMan® primer/probe set (Applied Biosystems). Endogenous mouse Beta-Actin (Mm02619580_g1 ...
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bioRxiv - Cell Biology 2020Quote: SSCs were separated from MEFs by gentle pipetting and total RNA was extracted from SSCs using TRIzol (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... The slides were washed with 2X SSC buffer containing 10% formamide in 2X SSC (ThermoFisher Scientific, Waltham, MA, USA), followed by hybridization buffer containing 10% dextran sulphate ...
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bioRxiv - Microbiology 2022Quote: ... TaqMan based RT-qPCRs were implemented using the AG-Path-One Step RT-PCR Kit (Ambion). Cycling was performed on a Bio-Rad CFX96 real-time PCR detection system (BioRad ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT–PCR (qRT–PCR) was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems) on the ABI7900HTsequence detector (Applied Biosystems) ...
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bioRxiv - Physiology 2022Quote: ... Reverse transcription PCR (RT-PCR) was performed with the Viia 7 Real-Time PCR System (Life Technologies) using SYBR Green reagent (Life Technologies) ...
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bioRxiv - Physiology 2022Quote: ... Reverse transcription PCR (RT-PCR) was performed with the Viia 7 Real-Time PCR System (Life Technologies) using SYBR Green reagent (Life Technologies) ...
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bioRxiv - Physiology 2023Quote: ... Reverse transcription PCR (RT-PCR) was performed with the Viia 7 Real-Time PCR System (Life Technologies) using a SYBR Green reagent (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... OpenArray chips in total contained primers for 2429 targets were read on 12_K Flex RT-PCR machine (Thermo Fisher Scientific, Life Technologies Corporation, Grand Island, New York). Data analysis was performed through the online available Expression suit v 1.3 software (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The RT-PCR assays were conducted by using SuperScript III One-Step RT-PCR System with Platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA). Amplifications were performed in an Eppendorf Master Cycler Model 5345 (Eppendorf ...
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bioRxiv - Immunology 2021Quote: ... Beads were re-emulsified in an overlap extension RT-PCR mixture using the SuperScript III RT-PCR Kit (Thermo Fisher Scientific, Waltham, MA) with the incorporation of custom designed TCR cloning primers (Supplementary Table 2)53 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was isolated from wild type N2 animals using trizol and phenol-chloroform extraction followed by RT-PCR using Superscript III One Step RT-PCR system with Platinum Taq DNA polymerase kit (ThermoFisher Scientific cat #: 12574026) to prepare the cDNA ...