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Citations for Thermo Fisher :
1 - 50 of 67 citations for pNFkB DD tdTomato since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... DD medium consists of DMEM/F12 (Gibco) base ...
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bioRxiv - Cell Biology 2022Quote: ... Data were recorded with a Falcon III DD camera (Thermo Fisher Scientific) in linear mode at 29’000 × magnification ...
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bioRxiv - Cell Biology 2020Quote: ... data were recorded with a Falcon III DD camera (ThermoFisher Scientific) in linear mode at 29’000X magnification ...
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bioRxiv - Immunology 2024Quote: ... Human primary fetal tissue was maintained in differentiation medium (DD) consisting of DMEM/F12 (Gibco #11320033) base ...
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bioRxiv - Neuroscience 2023Quote: ... chicken anti-tdTomato (1:500, ThermoFisher, TA150089). The slices were then washed 4 times for 20 min each time in PBS 0.5% Triton X-100 and then incubated with the appropriate secondary antibodies for 2h at RT in a solution containing 0.5% Triton X-100 and 2%NGS in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse MHC-I FITC conjugated H2-Dd/H-2Kd clone 34-1-2S (Invitrogen #11-5998-82), mouse MHC-I PE conjugated H-2Kb clone AF6-88.5.5.3 (Invitrogen #12-5958-82).
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bioRxiv - Cancer Biology 2024Quote: ... 10,000 TdTomato+ and TdTomato-cells of the fibroblast enriched fractions were sorted directly into Tri reagent® (TR118, Fisher Scientific), and RNA was extracted according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... operating in data dependent MS/MS mode (dd-MS2) and the data were then processed using LipidSearch software v4.1.16 (ThermoFisher) in product search mode.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... rat anti-mCherry (used against tdTomato; ThermoFisher, #M11217), 1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: MDA-MB-231 were transfected with a lentiviral vector encoding the red fluorescent protein palmitoylated-tdTomato (tdTomato) using lipofectamine transfection reagent (Life Technologies). Supernatant recovered after 48h from 293T transfected cells was filtered by a 0.45□μm pore membrane and added to MDA-MB-231 plated cells supplemented with 4□μg/mL polybrene (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... sub-pallium and Dd region were dissected based on the DsRed signals and collected in Trizol (Thermo Fisher Scientific). 30 fish were used for one replicate and two replicates were made ...
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bioRxiv - Cell Biology 2023Quote: ... gene expression of tdTomato [TaqMan probe FAM - Mr07319439_mr (Thermofisher)] and GAPDH (Rufino-ramos et al ...
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bioRxiv - Cell Biology 2024Quote: ... TdTomato-positive cells were collected in TRIZOL (Thermo Fisher). RNA from tdTomato positive SRSF2 knockout and P95H mutant cells and reporter positive control cells was extracted using the Direct-zol RNA microprep kit (Zymo Research) ...
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bioRxiv - Neuroscience 2021Quote: ... For GAL4CIII::tdTomato we utilized Gateway cloning to combine the R83B04 (CIII) enhancer with CD4-tdTomato by LR reaction (Invitrogen ref: 12538-120). Transgenic flies carrying a 2nd chromosomal insertion of CIII:tdTomato (docking site ...
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bioRxiv - Cell Biology 2021Quote: ... operating in data dependent MS/MS mode (dd-MS2) and the data were then processed using LipidSearch software v4.2.21 (Thermo Fisher Scientific) in product search mode ...
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bioRxiv - Neuroscience 2024Quote: TdTomato-positive E14.5 MGE cells from Sst-Cre/tdTomato or Sst-Cre/tdTomato/Dnmt1 loxP2 were FAC sorted into 100 µL TRIzol™ reagent (Thermo Fisher Scientific, U.S.A.). Samples were mixed by inversion and stored at -80°C until processing ...
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bioRxiv - Biochemistry 2021Quote: ... was used in order to subclone two HNF-1A constructs (DD-DBD: residues 1 - 279, DBD: residues 83 - 279) into the Gateway donor vector pDONR221 (Invitrogen™). An N-terminal Tobacco-Etch Virus (TEV ...
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bioRxiv - Bioengineering 2024Quote: ... and the YAP/TAZ-TRE-tdTomato plasmid using Lipofectamine 2000 (Invitrogen). Cells were cultured for 2 days post-transfection ...
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bioRxiv - Cell Biology 2020Quote: Cells from one-day-old culture - at the mid-exponential growth phase - at an initial cell density of 1×105 cells / ml were seeded in 12-well plates (Nunc/DD Biolab, #55428) with 1 ml marine broth and incubated at 23 ºC ...
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bioRxiv - Biophysics 2022Quote: ... MEF cells containing Piezo1-tdTomato were passaged using TrypLE Express (ThermoFisher, 12604013) to dissociate the cells and were spun at 260g for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... and rat anti-mCherry (1:1000, ThermoFisher; used for mCherry and tdTomato). The secondary antibodies are Donkey anti-chicken 488 (1:1000 ...
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bioRxiv - Pathology 2023Quote: ... Residual human IgG in the DDS and in the ocular tissues was determined using goat anti-human IgG antibody (A-21089, Thermo Scientific, Waltham, MA, USA). The total number and area of cells expressing CD45 in corneal sections were determined using the “Analyze Particles” tool in ImageJ software ...
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bioRxiv - Cell Biology 2020Quote: WT cardiac fibroblasts were nucleofected with pCDNA3.1 tdTomato (Neon Transfection kit, Invitrogen, MPIK10025), diluted 1 in 2 with control cells (cell nucleofected with no DNA) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were washed with heated wash buffer (HCR Buffers set, Molecular Instruments) and three washes with 5xSCCT (20x sodium chloride sodium citrate; 7732-18-5, Invitrogen; + 0.1% Tween in dd H2O). Samples were pre-incubated with amplification buffer (HCR Buffers set ...
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bioRxiv - Plant Biology 2021Quote: pMET3:H2B-tdTomato and pMET3:H2B-GUS were generated using the Gateway Cloning System (Invitrogen). All PCR fragments were amplified by PCR using the Phusion High-Fidelity DNA Polymerase (Thermo) ...
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bioRxiv - Biophysics 2022Quote: ... Piezo1-tdTomato MEFs and mLSECs were washed with phenol red-free DMEM/F12 (Invitrogen, 25116001) thrice and incubated in imaging solution ...
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bioRxiv - Molecular Biology 2020Quote: ... the MERVL::tdTomato reporter was transfected into E14 cells using Lipofectamine 2000 (11668030, Thermo Scientific), and single colonies were isolated ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from isolated tdTomato+ KPT tumor cells using TRIzol (Thermo Fisher Scientific) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with Kif1A-tdTomato and EGFP-FRB-EEA1 using Lipofectamine 2000 (ThermoFisher, Cat #11668027). The following day ...
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bioRxiv - Molecular Biology 2022Quote: ... 200 ng PB-TetR-tdTomato and 200 ng pBroad3_hyPBase_IRES_tagRFPt40 with Lipofectamine3000 (Thermo Fisher Scientific, L3000008) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... An anti-tdTomato antibody was used with a Tyramide Signal Amplification Kit (B40931, Thermo Fisher Scientific) to visualize cancer cells ...
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bioRxiv - Neuroscience 2021Quote: ... and a trafficking signal prior to tdTomato or citrine fusion and inserted in the pcDNA3.1+ vector (Invitrogen) for expression ...
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bioRxiv - Biophysics 2022Quote: ... and tail from the embryo at embryonic days 10.5 (WT, Het, Piezo1-KO) or 12.5 (Piezo1-tdTomato) in Dulbecco’s PBS (Gibco) supplemented with 33 mM D-(+)-glucose (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: tdTomato positivity was assessed by flow cytometry using IGI facilities on the Attune NxT (Thermo Fisher, AFC2). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... TdTomato-positive cells were either collected in 100 µL of cold TRIzol™ (Thermo Fisher Scientific, U.S.A.) for subsequent RNA sequencing or in 100 µL of cold HBSS (w/o phenol red ...
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bioRxiv - Cell Biology 2022Quote: ... from Hopx-CreERT2::R26-LSL-tdTomato or Lgr5-eGFP-IRES-CreERT2 mice into TRIzol LS (Thermo Fisher Scientific). After phase separation using the standard TRIzol LS protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Whole-tissue microscopy images of tdTomato fluorescence were acquired using an EVOS FL Auto 2 Imaging System (Invitrogen) before hematoxylin/eosin (H&E ...
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bioRxiv - Neuroscience 2023Quote: ... RV11.G-H2B:GFP and RV11.G-tdTomato were recovered in B7GG cells by Lipofectamine 2000 (Thermo Fisher Scientific) transfection with pcDNA-SADB19N ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells stably expressing NFAT5Δ547-tdTomato (NFAT5Δ) and GFP were established by transfecting with NFAT5Δ in pcDNA6 vector (Invitrogen) and EGFP in pcDNA4/TO vector (Invitrogen) ...
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bioRxiv - Genomics 2022Quote: ... mouse pancreatic islets isolated from RipCre-tdTomato mice were trypsinized for 7 minutes with 1 mM of trypsine (Gibco). Dissociated pancreatic cells were directly run into an Influx sorter (Becton Dickinson® ...
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bioRxiv - Cancer Biology 2022Quote: ... PDGFRα+ GFP- tdTomato+ were selected and sorted directly into a lysis solution from Arcturus PicoPure RNA Isolation Kit (Thermofisher). For sample validation ...
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bioRxiv - Neuroscience 2024Quote: ... and GFP-positive and TdTomato-negative cells (approximately 100,000-200,000 cells/sample) were collected directly in 1mL of TRIzol-LS (Invitrogen, 10296010) and frozen at -80°C.
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bioRxiv - Neuroscience 2020Quote: ... tdTomato sequence was added to the genome) using the cDNA labels combined with the 96 ERCC spike-in controls (Ambion). Median assignment rate was 61% (±10% S.D.) ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Neuroscience 2020Quote: The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... were co-transfected with plasmids encoding either EB3-GFP and DsRed or EB3- tdTomato and eGFP using Lipofectamine 2000 (Invitrogen). Live cell imaging was performed 24-48 h after transfection in complete HBSS media (HBSS ...
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bioRxiv - Immunology 2021Quote: ... Cells were transfected with 3 μg pGuide-it-tdTomato with inserted TLR5-specific guides and 12 μl Lipofectamine 2000 transfection reagent (Invitrogen) per well ...
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bioRxiv - Plant Biology 2022Quote: ... Entry clone with BFN1 promoter was recombined together with pEN-L1-NLS-tdTOMATO-L2 (Gateway) into destination vector pK7m24GW2 (Gateway) plasmid following the manufacturer instructions (Gateway, Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... ZMEL cells were nucleofected with the ubb:plin2-tdtomato plasmid using the Neon Transfection System (Thermo Fisher, Waltham, USA, Catalog #MPK10096), selected for two weeks in blasticidine supplemented media at 4 μg/μL (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and primers (Table 1) targeting the tdTomato-WPRE sequence (AAV) or the R26 locus (nuclei) on a QuantStudio5 (Applied Biosystems) instrument ...