Labshake search
Citations for Thermo Fisher :
1 - 50 of 124 citations for pEGFP C2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... or miniSINEUP-GFP swapping mutant plasmids at a molar ratio of 1:4.3 for long SINEUPs GFP [0.19 pmol pEGFP-C2 + 0.81 pmol of long SINEUP] and 1:4.6 for miniSINEUP-GFP [0.19 pmol pEGFP-C2 + 0.87 pmol of miniSINEUP-GFP] using 10 µL of Lipofectamine2000 (Thermo Fisher Scientific, 11668-019). After 24 h of transfection ...
-
bioRxiv - Biophysics 2020Quote: ... Alexa 555 C2 maleimide or Alexa 647 C2 maleimide (ThermoFisher, #A10254, #A20346, #A20347) as previously described37 ...
-
bioRxiv - Cancer Biology 2020Quote: ... pEGFP-cavin3 or pEGFP-CAV1 DNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Alexa 555 C2-malemide (Invitrogen, A20346) for TDP-43 ΔLCD ...
-
bioRxiv - Biochemistry 2021Quote: ... or AlexaFluor 647 C2-Maleimide (Invitrogen) (AF647) ...
-
bioRxiv - Biochemistry 2023Quote: ... Alexa Fluor 680 C2 maleimide (ThermoFisher), IRDye 680LT maleimide (Li-Cor) ...
-
bioRxiv - Neuroscience 2021Quote: ... were stochastically labeled with Alexa 555 C2 and Alexa 647 C2 maleimide (Thermo Fisher Scientific, Waltham, MA) fluorescent dyes at 10x molar excess of the protein concentration in TBS overnight on a rotating platform at 4℃ ...
-
bioRxiv - Biochemistry 2020Quote: ProIGF2 was labeled with a stochiometric concentration of AlexaFluor 555 C2 maleimide or AlexaFluor 647 C2 maleimide (Invitrogen) for 4hr at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... and Alexa 647 C2-malemide (Invitrogen, A20347) for Hsp70 and Hsc70 ...
-
bioRxiv - Neuroscience 2021Quote: ... Alexa 647-C2 maleimide purchased from Invitrogen. Immobilon PVDF membrane from Merck ...
-
bioRxiv - Molecular Biology 2023Quote: ... Alexa Fluor 647 C2 maleimide (ThermoFisher Scientific) was added to 10× molar excess and the labeling reaction was incubated overnight at 4°C in the dark ...
-
bioRxiv - Cell Biology 2021Quote: ... RAD54/RAD51AP1 double KO cells were transfected with peGFP-C1 or peGFP-RAD51AP1 and Lipofectamine2000 (Invitrogen). Twenty-four hours after transfection ...
-
bioRxiv - Biophysics 2020Quote: ... and conjugated to AF555 C2-maleimide (ThermoFisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... the fluorescent dye (AF-555 C2 maleimide, ThermoFisher) dissolved in DMSO was added in a molar ratio of 5:1 of (dye:vimentin) ...
-
bioRxiv - Biophysics 2024Quote: ... and 2.4 μM Alexa 647-C2-maleimide (Invitrogen) in 3 mL of ECB buffer ...
-
bioRxiv - Biophysics 2024Quote: ... or maleimide Alexa Fluor 647 C2 maleimide (ThermoFisher) was added dropwise whilst vortexing to a 200 μL solution of minibinder at 1 mg/ml in [20 mM Tris pH 7.4 ...
-
bioRxiv - Cancer Biology 2024Quote: TRAMP-C2 was cultured in DMEM (Gibco, 11965092), Bovine Insulin (0.005mg/ml ...
-
A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... pEGFP-N1-M1 and pEGFP-N1-M3 plasmids were transfected into cancer cell lines using LipofectamineTM 3000 (Invitrogen). Six hours after transfection ...
-
bioRxiv - Biophysics 2020Quote: ... Alexa Fluor™ 647 C2 Maleimide (A20347, Invitrogen™) was used to label the only cysteine of IDRFBP17 ...
-
bioRxiv - Biophysics 2024Quote: ... 10 μL of Alexa Fluor 647 C2 maleimide (ThermoFisher) was added dropwise to 1 mL of purified binder ( 10 μM ...
-
bioRxiv - Genetics 2024Quote: iPSC-CMs were stained on day 14 post-differentiation using RV-C2 Troponin T, cardiac type78 (DSHB, RV-C2) and a nuclear Hoechst dye (Thermo Scientific, Cat.62249) (Figure 7C) ...
-
bioRxiv - Biophysics 2020Quote: ... and Alexa Fluor 647 C2 Maleimide (ThermoFisher Scientific, Invitrogen, A20347). After labelling with Alexa Fluor 647 ...
-
bioRxiv - Biophysics 2020Quote: ... and Alexa Fluor 647 C2 Maleimide (ThermoFisher Scientific, Invitrogen, A20347). After labelling with Alexa Fluor 647 ...
-
bioRxiv - Biophysics 2022Quote: ... Alexa Fluor 647 C2 maleimide was purchased from Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: ... 500 μM Alexa Fluor 647 C2 Maleimide (ThermoFisher, for polySUMO), Atto 647N Maleimide (Sigma ...
-
bioRxiv - Biophysics 2023Quote: ... Alexa Fluor 647 C2 maleimide was purchased from Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... or pEGFP-ARα1b using Lipofectamine 3000 (Thermo Scientific). Before assay ...
-
bioRxiv - Cancer Biology 2022Quote: ... The next day cells were transfected with pEGFP-C3 KRAS4B 12V or pEGFP-C3 NRAS 12D using Lipofectamine 3000 (Thermo Fisher) following manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: ... A549 cells were transiently transfected with FUS-pEGFP C1 or FUS ΔNLS-pEGFP C1 using Lipofectamine LTX with PLUS Reagent (Thermo Fisher Scientific). A549 cells stably expressing eGFP-FUS (1 to 526) ...
-
bioRxiv - Cell Biology 2021Quote: ... 500 μM Alexa Fluor 647 C2 Maleimide (ThermoFisher, for N-WASP), Alexa Fluor 488 C5 Maleimide (ThermoFisher ...
-
bioRxiv - Biochemistry 2024Quote: ... and AlexaFluor®647-C2-Maleimide (F-MAL2, ThermoFisher, UK, #A20347) fluorophores ...
-
bioRxiv - Neuroscience 2021Quote: ... and subsequently labelled with AlexaFluor555 C2 maleimide (Thermo Fisher Scientific, A-20346). 5-7.5 μg of HCT-555 alone ...
-
bioRxiv - Neuroscience 2022Quote: DNAJB6 was labeled with Alexa Fluor™ 555 C2 Maleimide (Thermo Scientific) following the manufacturer’s guidelines.
-
bioRxiv - Biophysics 2024Quote: ... PolyPRM was labeled using Alexa Fluor 647 (AF647) C2 Maleimide (Molecular Probes) using the manufacturer protocol.
-
bioRxiv - Biochemistry 2024Quote: ... and Alexa Fluor™ 555 C2-Maleimide (A20346) were obtained from Invitrogen. Antibodies used for immunoblotting ...
-
bioRxiv - Biophysics 2022Quote: ... pEGFP-N3-Rph3A-K3 by using LipofectAmine 3000 (Invitrogen, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... pEGFP-E1E2 vector was transfected using TurboFect (Thermo Fisher Scientific) reagent following manufacturer’s instructions ...
-
The node of Ranvier influences the in vivo axonal transport of mitochondria and signalling endosomesbioRxiv - Neuroscience 2024Quote: ... cleaved and subsequently labelled with AlexaFluor555 C2 maleimide (Thermo Fisher Scientific, A-20346). Mice were anaesthetised using isoflurane ...
-
bioRxiv - Biophysics 2024Quote: ... The NMDA receptors were labeled using 600 nM Alexa 555-C2-maleimide (Invitrogen) and 2.4 μM Alexa 647-C2-maleimide (Invitrogen ...
-
bioRxiv - Biochemistry 2024Quote: ... and 250 μM Alexa Fluor™ 647 C2 Maleimide (acceptor, Thermo Fisher Scientific) shaking at room temperature for at least 2 hours ...
-
bioRxiv - Microbiology 2020Quote: ... or pEGFP-N-OtDUBGEF-E572A-GFP with Lipofectamine 3000 (Invitrogen L3000001), and media was replaced 5 hours post-transfection.
-
bioRxiv - Biochemistry 2020Quote: ... Next 3-fold excess of Alexa Fluor 647 C2 Maleimide (Thermo Fisher Scientific #A20347) was mixed with the solution and the mixture was incubated at room temperature for 1 hour ...
-
bioRxiv - Biophysics 2021Quote: ... of Alexa Fluor™555- and Alexa Fluor™647-C2 maleimide (ThermoFisher Scientific). Excess dyes are removed again by gravity gel filtration using a PD Min-Trap G-25 column ...
-
bioRxiv - Cancer Biology 2020Quote: ... RG/C2s were cultured in Dulbecco’s Modified Eagles Medium (DMEM) (Life Technologies, Paisley, UK) and supplemented with 20% foetal bovine serum (FBS)(Life Technologies ...
-
bioRxiv - Molecular Biology 2022Quote: ... 100 µM Alexa Fluor 647 C2 Maleimide (10-fold molar excess, Thermo Fisher Scientific) was added and rotated at 4°C overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... Alexa maleimide 647 C2 and Alexa FluorR 488 Halo ligand were purchased from Thermofisher Scientific (Waltham ...
-
bioRxiv - Cell Biology 2024Quote: The various thiolations of tWGA were conjugated with AlexaFluor 647 C2 maleimide (Invitrogen A20347) in a 10-fold molar excess for 1.5 hours at room temp ...
-
bioRxiv - Cell Biology 2023Quote: ... and subcloned into the pEGFP-N2 vector with GATEWAY system (Invitrogen, USA). GST constructs were transformed into Escherichia coli JM109 by heat shock and expressed recombinant proteins bound to glutathione (GSH)-sepharose beads were prepared according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... with plasmids expressing wildtype (WT) or mutant TRPM2 channel and pEGFP-N1 (Invitrogen) that expresses enhanced green fluorescent protein (GFP ...
-
bioRxiv - Immunology 2022Quote: ... SW480 cells were transfected with pEGFP-LC3 using the Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s instructions ...