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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for hsa mir 222 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... hsa-mir-423-5p (Thermofisher) served as endogenous control for analysis of miRNA expression ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-hsa-miR-Mir9-5p (Ambion, #17000), and Anti-miR negative control (Ambion ...
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bioRxiv - Neuroscience 2024Quote: ... Primers used were: rno-miR-155-5p (RT:002571, ThermoFisher Scientific), and rno-miR-674-3p (RT:001956 ...
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bioRxiv - Neuroscience 2021Quote: ... The probes hsa-miR-499a-5p (Alexa Fluor 546; Thermo Fisher) and CamK2 (Alexa Fluor 488 ...
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bioRxiv - Immunology 2020Quote: ... Probes for TaqManTM MicroRNA Assay (hsa-miR-424-3p, hsa-miR-424-5p, U6 snRNA control) were purchased from ThermoFisher. All the steps were performed according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), or 100 nM FlexiTube siRNA to ARF6 (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), or 100 nM FlexiTube siRNA to ARF6 (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Taqman miRNA assays for the housekeeping control gene U6 snRNA (#001973) and hsa-miR-21-5p (which also recognizes mmu-miR-21a-5p) (#000397) were purchased from Thermo Fisher. qPCR was performed using Taqman Universal Master Mix II ...
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bioRxiv - Genomics 2023Quote: ... TaqMan™ MicroRNA Assay hsa-miR-423-5p (ThermoFisher, AssayID 002340, 4427975), and TaqMan™ Fast Advanced Master Mix (ThermoFisher ...
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bioRxiv - Physiology 2020Quote: ... The multiplex RT primer pool consisted of primers for miR-150-5p and U6 (Thermo Fisher Scientific). No-template samples where included as negative controls.
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bioRxiv - Physiology 2020Quote: mirVana hsa-miR-150-5p (ID MC10070) mimic or scrambled miRNA control (Ambion) in complex with DACC lipoplex preparation (Silence Therapeutics)(Fehring et al. ...
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bioRxiv - Neuroscience 2022Quote: ... we used hsa-miR-423-5p (suggested as endogenous control by ThermoFisher, Inc) for PD ...
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bioRxiv - Molecular Biology 2024Quote: ... 20nM hsa-miR-129-5p miRIDIAN mimics (miR-129 mimics) or mimic negative control (mimics ctrl)(ThermoFisher Scientific); 20nM has-miR-129-5p miRIDIAN hairpin inhibitor (miR-129 inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... or hsa-miR-181-5p) (Bioneer) or with a Pre-miR miRNA Negative Control (Bioneer) using Lipofectamine 3000 (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: ... and primers specific for mmu-miR-147-3p (alias, hsa-miR-147b; Applied Biosystems). The relative abundance of EV miRNA was determined by TaqMan PCR using TaqMan Universal PCR Master Mix II and TaqMan primers and probes from Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... Capan-1 and Hs 766T were transfected with 100 nM Pre-miR Precursor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218 (Ambion ...
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bioRxiv - Cancer Biology 2020Quote: ... Each cell line was transfected for 72 hours with 100 nM Pre-miR Precursor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion ...
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bioRxiv - Cancer Biology 2020Quote: Capan-1 and Hs 766T were transfected for 72 hours with 100 nM Pre-miR Precursor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion ...
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bioRxiv - Biochemistry 2023Quote: ... with specific miR inhibitors (MH10203 for hsa-miR-22-3p and MH13382 for hsa-miR-1220-3p; Thermo Scientific) using Lipofectamine RNAiMax ...
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bioRxiv - Molecular Biology 2020Quote: ... and miR-183-5p (Applied Biosystems). Reverse transcription products were then amplified by quantitative PCR (95 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... as previously described (48) using the following primers: hsa-miR-21 (Thermofisher #0003970) and Z30 (Thermofisher #001092) ...
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bioRxiv - Neuroscience 2021Quote: ... transfected with the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p or Neg Control 1, Thermo Fisher) for 5-6 days ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal neurons were co-transfected with 300ng of Cav1.2-HA at DIV6 and 10nM of the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p and Neg Control 1, Thermo Fisher), and processed for immunostaining 13 days after ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal neurons were transfected at DIV 5 with 100ng of GFP alone or co-transfected with 10nM of the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p and Neg Control 1, Thermo Fisher). After 5 days of expression ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal neurons were co-transfected at DIV 5 with 10nM of the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p or Neg Control 1, Thermo Fisher) and 100ng of the Cacnb2 3’UTR luciferase reporters ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampal neurons were transfected at DIV7 with 10nM of the miR-499-5p or control mimic (Ambion™ Pre-miR miRNA Precursor: miR-499-5p and Neg Control 1, Thermo Fisher) with the Lipofectamine RNAiMAX reagent (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: mirVana™ miRNA Mimics and Inhibitors were used for hsa-miR-181a-5p overexpression/inhibition (Ambion™ - 4464066 and 4464084). HeLa cells were transfected with corresponding mirVana™ miRNA in 24 well plates at a density of 20.000 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: ... both shβ7 cells and H295R cells were transfected with control miRNA mimics or hsa-miR-139-5p mimics (Thermofisher, #MC11749) at a final concentration of 10nM ...
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bioRxiv - Cell Biology 2022Quote: ... for miR-375 was quantified by TaqMan Assays using the TaqMan MicroRNA Reverse Transcription Kit and hsa-miR-375 primer sets (Thermo Fisher Scientific, 000564). MiR-375 levels were normalized to miR-U6 expression ...
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bioRxiv - Pathology 2020Quote: ... miR-155-5p or its control scrambled miR (Thermo Scientific Dharmacon) using Effectene (Qiagen ...
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bioRxiv - Genomics 2023Quote: The mimic-miR-155-5p (Thermo Fisher, mirVanaTM miRNA Mimic ...
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bioRxiv - Neuroscience 2022Quote: ... Reverse-transcriptase-specific primers for mmu-miR-335-5p (Applied Biosystems miRNA assay ID 000546) were used and real-time quantitative PCR performed using TaqMan miRNA assays (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... Calu-3 cells were seeded at 2.5×105/ml with 100 or 300 nM of hsa-miR-145-5p miRNA precursor (PM11480, Ambion, Thermo Fisher Scientific). After 72 h ...
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bioRxiv - Genetics 2020Quote: ... hsa-miR-147b-3p and hsa-miR-210-3p levels were assessed by TaqMan Advanced miRNA Assays (Thermofisher) and TaqMan Fast Advanced miRNA master mix (Thermofisher) ...
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bioRxiv - Neuroscience 2023Quote: ... hsa-miR-221 (Thermo Fisher Scientific #000524) and hsa-miR-182 (Thermo Fisher Scientific #002334) ...
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bioRxiv - Cancer Biology 2021Quote: ... Q-RT-PCR was performed using TaqMan primers/probe sets (Applied Biosystems) and analyzed as transcript copies per 50 ng RNA expressed relative to RNAPII expression as previously described (33).
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bioRxiv - Cell Biology 2021Quote: ... and miR-34a-5p (ThermoFisher, Assay ID 000426). 1ul of cDNA was used for real-time PCR using TaqMan™ Universal PCR Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... Calu-3 cells were seeded at 2.5×105/ml with 100 or 300 nM of hsa-miR-145-5p miRNA precursor (PM11480, Ambion, Thermo Fisher Scientific). After 72 h ...
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bioRxiv - Cell Biology 2022Quote: ... and mirVana® hsa-let7b-5p miRNA mimic (MC24132, ThermoFisher) (50 nM ...
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bioRxiv - Cell Biology 2020Quote: ... TaqMan Fast Universal PCR Mastermix and TaqMan miRNA Assays primers were used to detect miR-23a-3p and miR-10b-5p (QuantStudio 12K Flex, Thermo Fisher Scientific). All reactions were run in triplicate and results were expressed as 2−ΔΔCt ...
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bioRxiv - Genomics 2024Quote: ... and miR-1 (Life Technologies, assay name: hsa-miR-1, cat# 4427975) following the manufacturer’s recommendations (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... and hsa-miR-182 (Thermo Fisher Scientific #002334). Relative expression of miRNAs analyzed in triplicates were normalized to the levels of Z30 and expressed as 2−ΔΔCt.
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using Taqman probes and primer sets (Applied Biosystems) specific for CD36 (assay ID Mm00432398_m1) ...
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bioRxiv - Cell Biology 2019Quote: ... 25 mM of miR-15a-5p (Ambion, 4464066 - MC10235) and miR-1 positive control (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... Synthetic miR-324-5p and Decade Markers System (Ambion) were loaded as size markers ...
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bioRxiv - Cancer Biology 2022Quote: ... Pre-miR-129-5p was purchased from Thermo Fisher Scientific (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: Cells at 60% confluence were transfected with 40 nM of mirVana™ miRNA mimic of miR-9-5p (miR-9-5p) (4464066, Ambion, Carlsbad, CA) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: Primary cortical neurons were transfected in triplicates with 100ng of pmiR-Glo-plasmids containing wildtype or mutant 3’UTRs of Mdga1 or Shank2 together with pre-miR-218-5p duplex (0.3125pmol for Mdga1 and 1.25pmol for Shank2, Ambion Pre-miR-218-5p Precursor) or respective quantities of pre-miR negative control duplex (Ambion ...
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bioRxiv - Molecular Biology 2019Quote: ... miRNA mimics and the miR-100 isoforms miR-100-5p and miR-100-3p (all from Life Technologies) using 15 pmol PepMute reagent (SignaGen Labs ...
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bioRxiv - Neuroscience 2022Quote: ... hsa-miR-10 (Applied Biosystems miRNA assay ID 000387), hsa-miR-129 (Applied Biosystems miRNA assay ID 000590 ...