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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... using a ratio of 1 µg trypsin to 50 µg sample protein as quantified using a Pierce BCA Protein Assay Kit (ThermoFisher). Samples were digested overnight at room temperature using a rotor for mixing and then placed in a speed vacuum to reduce sample volume ...
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bioRxiv - Biochemistry 2022Quote: ... reaction mixtures containing approximately 5 μM protein in DSF buffer (50 mM Na2HPO4, 150 mM NaCl, pH 7.4) and 1x Protein Thermal Shift Dye (Thermo Fisher) were heated from 20 °C to 99 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide yield from each sample was determined to be approximately 20 µg based on BCA Protein Assay (ThermoFisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... The peptide concentrations were determined by BCA assay using the Pierce Rapid Gold BCA Protein Assay Kit (ThermoFisher Scientific), and the protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Microbiology 2023Quote: ... 70 ng of peptides according to the results of the Invitrogen Qubit protein assay (Thermo Fisher Scientific, Darmstadt, Germany) supplemented with 40 fmol of Hi3 Phos B standard for protein absolute quantification (Waters ...
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bioRxiv - Immunology 2023Quote: A cell-based microplate assay was used to measure intracellular free zinc ions (Zn2+) using the cell-permeable fluorescent probe FluoZin-3 AM (Invitrogen, #F24195). BMDMs were plated into 96-well clear-bottom black polystyrene microplates (Corning ...
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bioRxiv - Plant Biology 2020Quote: ... Tryptic peptide samples were quantified by a colorimetric peptide assay (Pierce, Thermo Fisher) and aliquots of 200 ng were injected by LC-MS/MS ...
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bioRxiv - Developmental Biology 2020Quote: ... peptide concentrations were quantitated by the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher).
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bioRxiv - Bioengineering 2020Quote: ... Peptide concentration was determined with the Pierce Peptide Quantitation Kit (Thermo Fisher Scientific) and all samples were normalized to 0.1 g/L in 100 μL total volume ...
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bioRxiv - Biochemistry 2021Quote: ... peptides were quantified with a quantitative colorimetric peptide assay (ThermoFisher Scientific / Pierce, #23275). Fifty µg of protein was digested with 1 µg trypsin (Thermo ...
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bioRxiv - Systems Biology 2020Quote: ... The final peptide concentration was measured using Peptide Assay (Thermo Fisher Scientific, USA) on a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Then the peptides were desalted using peptide desalting spin columns (Thermo Fisher, 89852) before LC-MS/MS analysis on the Orbitrap Fusion mass spectrometer (Thermo Fisher) ...
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bioRxiv - Systems Biology 2020Quote: ... We measured resulting peptide concentrations with the Pierce Quantitative Fluorometric Peptide Assay (ThermoFisher) kit for standard samples ...
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bioRxiv - Biochemistry 2020Quote: ... Peptide concentrations were determined using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific). For the eight protein mixture ...
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bioRxiv - Systems Biology 2020Quote: ... Peptide concentration was measured with Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Fisher). In the Easy nanoLC-Q Exactive HFX setup ...
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bioRxiv - Physiology 2020Quote: ... Peptide concentrations were measured using the Quantitative Colorimetric Peptide assay kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, 23275).
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bioRxiv - Immunology 2022Quote: ... The resulting peptides were quantified by fluorometric peptide assay kit (Thermo Fisher Scientific) before mass spectrometry analysis.
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bioRxiv - Cancer Biology 2023Quote: ... Peptide samples were purified using Pierce peptide desalting spin columns (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Peptides were desalted using the Pierce™ Peptide Desalting Spin Columns (Thermo Scientific). TMTpro 16plex Label Reagent Set was used for labeling according to the manufacturer (Pierce,) ...
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bioRxiv - Systems Biology 2024Quote: ... peptide concentration was determined utilizing a colorimetric peptide assay kit from Thermo Fisher Scientific.
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bioRxiv - Neuroscience 2023Quote: ... Peptides concentration was measured by Quantitative Colorimetric Peptide Assay Kit (ThermoFisher, Cat# 23275). Combined peptides were dried in a vacuum concentrator and ready for liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Neuroscience 2023Quote: ... Peptide concentration was measured by Quantitative Colorimetric Peptide Assay Kit (ThermoFisher, Cat# 23275). In the case of AIS developmental TMT experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... Digested peptides were quantified with the Quantitative Fluorometric Peptide Assay kit (Thermo Scientific), normalized to 0.1 μg/μL and stored at -20°C prior to analysis on an EASY-nLC 1200 Liquid Chromatograph (Thermo Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... The plate was in-cubated for 1 h at room temperature with Pierce Protein-Free Blocking Buffer (Thermo Fisher Scientific) and again washed with PBS three times ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated with an anti-TasA antibody (rabbit) at a 1:20,000 dilution in Pierce Protein-Free (TBS) blocking buffer (Thermo Fisher). A secondary anti-rabbit IgG antibody conjugated to horseradish peroxidase (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... 100-200 mg of the remaining protein sample was added to a 50 mL 50% slurry of Neutravidin-conjugated agarose beads (Thermo Fisher Scientific) that was pre-washed 3X in lysis buffer ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... Blocking was performed for 20 min with Superblock® T20 (PBS) blocking buffer (Thermo Scientific) with 1% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was blocked with a blocking agent (SEA BLOCK Blocking, Thermo Scientific, Cat # UH2788881) for one hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... followed by blocking for endogenous avidin and biotin (avidin/biotin blocking kit, Invitrogen, MA, USA) per manufacturers instructions ...
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bioRxiv - Immunology 2023Quote: ... After blocking the slides for an hour in SuperBlockTM blocking buffer (Thermo Fisher cat#37515) the slides were incubated in antibody cocktail solution (Antibody Panel described in Supplementary file 1 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were again washed twice with PBS followed by blocking with blocking buffer (DMEM (Gibco) +10% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... Separation of peptides was achieved by use of an Acclaim Pepmap RSLC analytical column (C18, 100 Å, 75 μm. × 50 cm (Thermo Scientific)) ...
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bioRxiv - Cell Biology 2021Quote: One ug of each sample with 50 femtomole of heavy labeled Peptide Retention Time Calibrant (PRTC) mixture (Thermo Fisher Scientific, cat # 88321) was loaded onto a 30 cm fused silica picofrit (New Objective ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were separated by a PepMapTM RSLC C18 EasySpray column (2 µm, 100Å, 75 µm x 50 cm) (Thermo Fisher Scientific, UK), operating at 50°C ...
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bioRxiv - Microbiology 2020Quote: ... before peptide separation on a nanoscale PepMap 100 C18 nanoLC column (3 μm, 100 Å, 75 μm i.d. × 50 cm, Thermo Fisher Scientific) at a flow rate of 0.2 μL per min using a 120 min gradient comprising 100 min from 4% to 25% of solvent B and 20 min from 25% to 40% of solvent B (solvent A consisted in 0.1% formic acid in water ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides were separated on an EASY Spray C18 column (50 cm x 75 cm, 2 µm particle size) (ThermoFisher Scientific, Toronto) using an EASY nLC-1200 ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were separated on an EASY-Spray PepMap RSLC C18 column (2 µm particle size, 75 µm × 50 cm; Thermo Scientific, ES801) heated to 55 °C using a flow rate of 400 nL/min ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were separated on an Acclaim PepMap C18 column (id 75 μm, length 50 cm, particle size 2 μm, Thermo Fisher Scientific) using a 10%-45% gradient of solvent B (80% acetonitrile ...
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bioRxiv - Cancer Biology 2023Quote: ... Eluted phosphopeptide samples from both enrichments were pooled and fractionated into 14 fractions by increasing ACN concentration from 7 % to 50 % using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). Peptide separations of the whole proteome sample were performed using basic reversed-phase chromatography (bRP-LC ...
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bioRxiv - Plant Biology 2023Quote: ... each sample containing 25 μg of peptide in 50 mM TEAB buffer was combined with its respective 10-plex TMT reagent (Thermo Fisher Scientific) and incubated for 1 h at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Peptides were separated on an analytical column (Easy-Spray™ PepMap™ RSLC C18, 2 μm, 75 μm × 50 cm, Thermo Scientific) at a voltage of about 2.4 kV and at a flow rate of 0.3 μL/min with mobile phases of 0.1% FA in water and in 95% ACN using a linear gradient ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: 1.5 µg trypsinized peptides were dissolved in 50 mM TEAB pH 8.5 and labelled using the TMT10plex Isobaric Labeling Reagent Set (Thermo Fisher Scientific, 90111). Each sample was labeled with TMT10plex reagents at a 20:1 label:peptide w/w ratio ...
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bioRxiv - Neuroscience 2023Quote: ... pellets were reconstituted in 50[µl of 0.1[N NaOH and kept for protein quantification using a BCA protein assay kit (Thermo Scientific Pierce BCA Protein Assay Kit, PI23227). Dialysate concentrations are expressed as ng/ml and tissular concentrations as ng/mg of total proteins.
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bioRxiv - Bioengineering 2020Quote: ... Slides were blocked in serum-based blocking buffer and incubated with primary antibody against MMP12 (MA5-32011, Invitrogen, 1:50, 20 μg/mL). Slides were washed in PBS (3×5 minutes) ...
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bioRxiv - Neuroscience 2020Quote: ... Biochemical analyses were performed on water-homogenised tissues (50 mg/mL) and protein content determined (BCA protein assay, Thermo Fisher #23227) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: For analysis of the soluble protein fraction 50 µg protein were reduced with 2.5 mM TCEP (tris-(2-carboxyethyl) phosphine hydrochloride) (Invitrogen, Thermo Fisher Scientific) at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... Eluted proteins and 50 μg of protein from whole cell lysate were resolved in 4-15% NuPAGE gradient gel (ThermoFisher, NP0335) using MOPS running buffer (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatants were subsequently clarified with 50 µL of magnetic beads coupled to protein G (Dynabeads Protein G-LS10003D, Thermo Scientific) at 4°C for 1 h with agitation ...