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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Zika Virus NS1 Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Infectious virus was reconstituted by transfecting ARPE-19 cells with BAC DNA and Lipofectamine 3000 (ThermoFisher L3000001); high titer stocks generated after three passages in ARPE-19 cells were used for this project ...
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bioRxiv - Microbiology 2023Quote: ... infected cells were stained with primary antibody (Vaccinia Virus Polyclonal FITC Antibody ThermoFisher PA1-73191, 1:1000) and Hoechst 33342 (1:10,000) ...
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bioRxiv - Biochemistry 2023Quote: ... virus was harvested by collecting supernatant and filtering it through 0.45µm PES sterile filter (Thermo Fisher, 2954545). Virus was concentrated by ultracentrifugation with a 20% sucrose cushion ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed twice with PBS and then incubated with virus suspended in infectious medium (DMEM; Invitrogen) supplemented with 35% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... This virus mixture was used to infect 1.8–2.4 liters of High Five cells (Thermo Fisher Scientific) at a cell density of 3 × 106 per ml for 60 h at 27°C ...
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bioRxiv - Cell Biology 2023Quote: ... from a cDNA library of HCT116 which was prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen), as described previously (Ninagawa et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells used for the production of virus were cultured in DMEM supplemented with 2mM glutamine (Gibco), 10% Tetracycline-free FBS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... cells were infected with virus diluted in 10 mL of Opti-MEM (ThermoFisher Scientific, Waltham, MA, USA) and incubated for 1 h at 37°C to allow for virus adsorption ...
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bioRxiv - Microbiology 2024Quote: ... and the virus was incubated with 25 μg of Alexa Fluor 488 NHS Ester (succinimidyl ester; Invitrogen) for 1 hour at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... half of the virus supernatant after the concentration step was digested with amplification grade DNAse I (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from 1010 virus particles using the PureLink TM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... All iPSCs were generated by Sendai virus reprogramming and clonal expansion using the CytoTune – iPSC 2.0 kit (ThermoFisher) to deliver the reprogramming factors ...
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bioRxiv - Cell Biology 2019Quote: Cholesterol concentration in purified virus was determined using Amplex™ Red Cholesterol Assay Kit (Molecular Probes, Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: Cholesterol concentration in purified virus was determined using Amplex™ Red Cholesterol Assay Kit (Molecular Probes, Thermo Fisher) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2019Quote: ... Ka539 cells transfected with a virus expressing luciferase reporter were cultured in DMEM/high glucose and IMDM (Gibco) supplemented with 10% FBS (Natocor) ...
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bioRxiv - Microbiology 2020Quote: ... The cell layers were fixed on-chip and subjected to immunofluorescence microscopic analysis for influenza virus NP (Invitrogen) and neutrophils (CD45 ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated for 2 hours with the virus and then washed twice with Neurobasal-A medium (Gibco) containing 2% B-27 (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified via qRT-PCR using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) on a QuantStudio 3 (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA extractions of 80 μL of virus stocks lyzed with 240 μL of TRIzol LS Reagent (Invitrogen) were performed in triplicate with the Direct-zol RNA MiniPrep kit (Zymo Research) ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... All the procedures follow the manufacturer’s instructions of the Applied Biosystems TaqMan Fast Virus one-step mastermix (ThermoFisher). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of RNA was retrotranscribed to complementary DNA using moloney murine leukemia virus reverse transcriptase (Thermo Fisher) and random hexamer primers (Roche) ...
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bioRxiv - Pathology 2021Quote: ... Total RNA from virus supernatants was extracted using the PureLink™ Viral RNA/DNA Mini Kit (Thermo Fisher) according to the manufacturer’s protocol and then converted to cDNA by SuperScript® III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... we exchanged the medium in the filtered virus collections three times with Opti-MEM I (1X) + GlutaMAX (Gibco) using an Amicon Ultra-15 column (100 kDa cutoff) ...
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bioRxiv - Microbiology 2022Quote: ... quantitative polymerase chain reaction (qPCR) was performed using TaqMan Fast virus 1 Step PCR Master Mix (Applied Biosystems); a standard curve was drawn with 2019_nCOV_N Positive control (IDT) ...
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bioRxiv - Immunology 2022Quote: ... Reverse-transcription of isolated RNA was performed using a Moloney murine leukemia virus-reverse transcriptase kit (Life Technologies) and oligo (dT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative real-time PCR was performed using a TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific), 2019-nCoV RUO Kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The virus was propagated to passage three on Vero E6 cells in Opti-MEM I (1X) + GlutaMAX (Gibco), supplemented with penicillin (10,000 IU/mL ...
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bioRxiv - Microbiology 2019Quote: FVG-R infections were performed by inoculating A549 cells with virus diluted in Opti-MEM I (Invitrogen, 31985070) supplemented with 0.2% FBS ...
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bioRxiv - Genetics 2021Quote: ... Samples KAB_3 and KAB_7 were reprogrammed using Sendai virus (CytoTune™-iPS 2.0 Sendai Reprogramming Kit, ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: Concentrated virus particles were treated with RNase A before extraction of RNA using Trizol LS (Ambion, Life Technologies). The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN ...
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bioRxiv - Evolutionary Biology 2020Quote: Concentrated virus particles were treated with RNase A before extraction of RNA using Trizol LS (Ambion, Life Technologies). The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
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bioRxiv - Microbiology 2021Quote: ... Transmissible gastroenteritis virus [TGEV] and Swine deltacoronavirus [SDCoV] (VetMAX™ PEDV/TGEV/SDCoV, Thermo Fisher Scientific, MA, USA) and Senecavirus A [SVA] (EXOone Seneca Virus Valley ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription of isolated RNA was performed using a Moloney murine leukemia virus-reverse transcriptase kit (Life Technologies) and oligo(dT ...
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bioRxiv - Synthetic Biology 2022Quote: ... undiluted SINV-containing supernatants were combined with the TaqMan™ Fast Virus 1-Step Master Mix (ThermoFisher, #4444434) and the appropriate primer-probe sets in technical triplicates ...
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bioRxiv - Microbiology 2022Quote: ... Premixes were prepared for each amplification reaction using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... C and S genes of the Hepatitis B virus (accession number: KY00323035) was ordered from GeneArt (ThermoFisher Scientific) and is available from Addgene (Addgene # 179155).
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bioRxiv - Neuroscience 2023Quote: ... C-Myc) introduced by Sendai virus according to the manufacturer’s instructions (Cytotune iPS 2.0 Sendai reprogramming kit, ThermoFisher) or by using an episomal method ...
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bioRxiv - Cell Biology 2023Quote: ... After a brief culture to expand the erythroblast population a Sendai virus based approach (CytoTune 2.0 Kit, Invitrogen) was used to express the Yamanaka factors without any genomic integration (27) ...
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bioRxiv - Microbiology 2023Quote: ... l ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for l h ...
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bioRxiv - Microbiology 2023Quote: ... The cell and virus lysates were Western blotted and probed with a goat polyclonal anti-gp120 antibody (Invitrogen), the 4E10 anti-gp41 antibody (NIH HIV Reagent Program ...
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bioRxiv - Biochemistry 2023Quote: Bacmid preparation and virus production were performed according to the Bac-to-Bac baculovirus system manual (Gibco, Invitrogen). Spodoptera frugiperda Sf9 cells at a density of 3 × 106 cells/ml were co-infected with baculoviruses encoding receptor and Gi trimer at the ratio of 1:1 ...
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bioRxiv - Biochemistry 2023Quote: Bacmid preparation and virus production were performed according to the Bac-to-Bac baculovirus system manual (Gibco, Invitrogen). Spodoptera frugiperda Sf9 cells at a density of 3 × 106 cells/ml were co-infected with baculoviruses encoding receptor and Gi trimer at the ratio of 1:1 ...