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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and gene-specific probes and reverse primers as follows ...
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bioRxiv - Immunology 2023Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and N gene-specific probe and reverse primer as previously described47:
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bioRxiv - Microbiology 2023Quote: ... containing 5 µl of template and TaqMan Fast Virus 1-step mastermix (Applied Biosystems). Primer sequences and concentrations and thermal cycling conditions for SARS-CoV-2 nucleocapsid 1 gene were as previously described (13) ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli strains were grown in Lucia Bertani (LB) medium (Fisher Scientific) with aeration at 37°C ...
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bioRxiv - Genetics 2020Quote: ... Escherichia coli strain DH5α was used according to the manufacturer’s recommendations (Invitrogen). Bacterial transformants were selected on LB agar ...
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bioRxiv - Microbiology 2019Quote: ... Escherichia coli strains were cultivated in LB medium (Thermo Fisher Scientific, USA) supplemented with appropriate antibiotics ...
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bioRxiv - Biochemistry 2020Quote: Human ASCT2 (hASCT2) was produced in Pichia pastoris X-33 strain (Invitrogen) in fermentor59 and purified using DDM and CHS (Anatrace ...
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bioRxiv - Biochemistry 2020Quote: ... The construct was expressed in Escherichia coli strain BL21(DE3) star (Invitrogen) as a fusion protein with a His tag ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were transformed in chemically competent bacteria strains Top10 (Thermo Fisher, C303003) and Stbl3 (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... strains genomic DNA concentrations were measured on a Qubit 3.0 fluorometer (Invitrogen) and diluted to 10ng/uL in Tris HCl 5mM for library preparation using a Nextera kit (Illumina) ...
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bioRxiv - Biochemistry 2022Quote: ... The PLP/DM20 plasmid was transformed into DH10Bac strain (Thermo Fisher Scientific) for bacmid production ...
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bioRxiv - Plant Biology 2022Quote: ... The bait and prey plasmids were transformed into yeast strain MaV203 (Invitrogen): MATα leu2-3,112 trp1-901 his3Δ200 ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged strains were taken from the 4000-GFP yeast library (Thermofisher), while knockout strains were taken from the Yeast Knockout Collection (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... falciparum 3D7 strain was cultured in RPMI 1640 (Invitrogen, Carlsbad, CA, USA) supplemented with 27.2 mg/L hypoxanthine (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid construction and amplification were performed in the bacteria strain TOP10 (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: pMalC2-rabaptin5 was transformed into the BL21S(DE3)pLysS bacterial strain (Invitrogen). Protein expression was induced with 0.3 mM IPTG for 2 hours at 37°C and recombinant MBP-rabaptin5 was purified with an amylose resin (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... all strains were grown in Luria broth (LB; Thermo Fisher Scientific, USA) for 18 h to stationary phase at 37°C with shaking (180 r.p.m.) ...
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In-silico enrichment of bacterial plasmids by chromosomal depletion using nanopore adaptive samplingbioRxiv - Genomics 2022Quote: Campylobacter strains were streaked on Columbia Blood agar (Oxoid, Thermofisher Scientific, USA) and incubated at 42 °C under microaerobic atmosphere ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli strain DH5α (E. coli DH5α, Thermo Fisher Scientific, Cat#18258012), E ...
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bioRxiv - Cell Biology 2023Quote: ... coli strain OP50 containing 500 nM MitoTracker Deep Red FM (Invitrogen M22426) until day 1 adults ...
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bioRxiv - Microbiology 2023Quote: ... coli strains grown overnight on Lennox Broth (LB) (Bacto Tryptone (#211699, Gibco) 10g/L ...
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bioRxiv - Microbiology 2023Quote: ISG20 was expressed in E.coli BL21(DE3) Rosetta/pLysS strain (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... TBEV strain Hypr was collected on a Titan Krios microscope (Thermo Fisher) operating at 300 kV aligned for parallel illumination in nanoprobe mode equipped with a Falcon3EC direct electron detector ...
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bioRxiv - Plant Biology 2023Quote: ... coli strain BL21 and purified using PierceTM glutathione magnetic agarose (ThermoFisher Scientific). The purified protein was digested with PreScission Protease (APEXBIO ...
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bioRxiv - Genomics 2023Quote: ... Alexa 488 conjugated Escherichia coli (K-12 strain) BioParticles (cat: E13231, ThermoFisher) was added at 10ug/well ...
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bioRxiv - Synthetic Biology 2023Quote: Yeast expression vectors (Table S3) were transformed into yeast strain INVSc1 (Invitrogen) and plated out onto uracil-free selection medium (1 g/L Yeast Synthetic Drop-out Medium Supplements without uracil ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-tagged strains were taken from the 4000-GFP yeast library (Thermofisher), RFP-tagged strains were taken from the localization database collection (Huh et al ...
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bioRxiv - Microbiology 2023Quote: Fusobacterium nucleatum strains were cultured in Columbia broth (CB, Fisher Scientific, MA) at 37 °C under anaerobic condition (0 % O2 ...
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bioRxiv - Biochemistry 2023Quote: ... Competent bacteria (Escherichia coli, strain TOP10, Thermo Fisher Scientific, cat. no. C404010) were transformed with the resulting recombination products ...
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bioRxiv - Cancer Biology 2021Quote: ... of Iowa) was prepared by diluting virus stocks to 400 p.f.u./µL into OptiMEM (Gibco) followed by addition of CaCl2 to a final concentration of 10 µM ...
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bioRxiv - Microbiology 2020Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075).
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed using the TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems). Assays were conducted on a QuantStudio5 Q-PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... The assay was performed using the TaqMan® Fast Virus 1-Step Kit (Life Technologies) and kit manual ...
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bioRxiv - Molecular Biology 2020Quote: ... a 20μL monoplex reaction contained 5μL of 4x TaqMan Fast Virus Master Mix (Thermo Fisher), 0.6µL of each primer (0.3μM final concentration each) ...
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bioRxiv - Immunology 2019Quote: ... two-fold virus stock dilutions were prepared in U-shaped 96-well plates (Thermo Scientific). The same volume of a 1% human O+ red blood cell suspension (Innovative Research ...
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bioRxiv - Microbiology 2019Quote: ... Other antibodies were obtained from commercial vendors as follows: Anti-coxsackie virus B3 antibodies (ThermoFisher), Anti-adenovirus A5 antibodies (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... RAW264.7 cells were then transduced with virus and selected using hygromycin at 10mg/ml (Invitrogen) to select for cells containing the shRNA plasmid.
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bioRxiv - Microbiology 2021Quote: ... and the number of virus particles was determined using a Nanodrop 2000 (Thermo Fisher Scientific). The adenovirus stocks were aliquoted and stored at -80°C.
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Neuroscience 2021Quote: ... The final buffer for the virus was: dPBS + 5% Glycerol + 0.001 Pluronic F-68 (Invitrogen). The AAV titre (1.71 × 1013 GC/ml ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqManTM Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... The qPCR for Sendai virus detection was performed using TaqMan Gene Expression Assays (Life Technologies). Quantitative RT-PCR was performed using SYBR® Green PCR Master Mix (Applied Biosystems™ ...
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bioRxiv - Cell Biology 2022Quote: Cell lines were generated using Sendai virus CytoTune-iPS 2.0 Sendai reprogramming kit (Thermo Fisher) for transfection of Yamanaka’s factors ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL of purified virus was analyzed with the Quant-it PicoGreen dsDNA Assay (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... concentrated virus was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies), which was then analyzed using the Pierce Silver Stain Kit (Pierce ...
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bioRxiv - Immunology 2022Quote: ... For these assays we used TaqMan Fast Virus 1- Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from amplified virus stock using Trizol reagent (Invitrogen; Cat no. #15596018). Reverse transcription was carried out using in-house produced Moloney murine leukemia virus (MMLV ...
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bioRxiv - Neuroscience 2021Quote: ... using TaqMan Fast Virus 1-Step Master Mix (PN4453800, Applied biosystems, Thermo Fisher Scientific, USA). PrimerTime primers and FAM-dye coupled detection probes were used for detecting Clstn3 mRNA level ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV, Invitrogen). PCR was performed in an AriaMx thermal cycler (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UKEi1-A was reprogrammed with a Sendai Virus strategy (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). These studies were approved by the Ethical Committee of the University Medical Center Hamburg-Eppendorf (Az ...