Labshake search
Citations for Thermo Fisher :
551 - 600 of 4133 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... RNA from virus-infected cells was isolated using PureLink RNA Mini Kit (Thermo Fisher Scientific), following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... genomic DNA was extracted from 1010 virus using the PureLinkTM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... The virus was dialyzed with PBS supplemented with 0.05% of Pluronic F68 (Thermo Fisher Scientific) using 100-kDa filters (Millipore) ...
-
Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus (MMLV) reverse transcriptase (#28025013, Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
-
bioRxiv - Plant Biology 2019Quote: ... 10 IU of Moloney murine leukemia virus RNase H+ reverse transcriptase solution (Thermo Fisher Scientific), and appropriate buffer containing deoxynucleoside triphosphates (dNTPs ...
-
bioRxiv - Cell Biology 2020Quote: ... Isolated total RNA was reverse transcribed with Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen) and oligo-dT primers ...
-
bioRxiv - Microbiology 2021Quote: ... MRC-University of Glasgow Centre for Virus Research) were maintained in Leibovitz’s L15 media (Gibco) containing 10% FBS (Gibco) ...
-
bioRxiv - Microbiology 2020Quote: Compound dilutions for virus treatment were performed in Minimum Essential Media (MEM) purchased by Gibco.
-
bioRxiv - Microbiology 2019Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075). HSV-1 expressing the FLAG-tagged N-terminal extension of UL54 virus were reconstituted and titrated on Vero 2-2 cells50 ...
-
bioRxiv - Microbiology 2019Quote: Concentrated virus aliquots were mixed with DiI (final concentration of 1 μM, Thermo Fisher, USA), vortexed and incubated for 1.5 h at room temperature (RT) ...
-
bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher) and 5 µl of isolated RNA as a template to detect a 132 bp sequence in the ORF1b/NSP14 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... expanded PBMCs were transduced with non-integrating Sendai reprogramming virus (Cytotune 2.0, Thermo Fisher, A16517) according to manufacturer’s instructions added to the Expansion Media ...
-
bioRxiv - Genomics 2020Quote: ... endometrium tissues fibroblast cells were transfected with a Sendai virus reprogramming kit (Life Technologies, A16517). The transfected cells were then plated onto Matrigel-coated culture dishes according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: Abelson virus-transformed pre-B cell lines were maintained in DMEM (Thermo Fisher #11960-077) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Neuroscience 2021Quote: ... The viral DNA samples were prepared for quantification by treating the virus with DNaseI (Invitrogen) and Proteinase K ...
-
bioRxiv - Microbiology 2020Quote: The nasal swab was initially stored in virus transport medium [Hank’s Balanced Salt Solution (Gibco) containing 2% FCS (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2022Quote: ... qPCRs were set up using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher) and run on an ABI 7500 Fast Real-time PCR System (Applied Biosystems/Thermo Fisher) ...
-
bioRxiv - Microbiology 2022Quote: ... the virus inoculum was previously activated with trypsin (200 μg/ml, Gibco Cat. 27250-18) for 1 h at 37 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Virus was washed and eluted in Hank’s balanced salt solution (Gibco™, Dublin, Ireland) containing 0.014% Tween-20 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: Purified virus stocks were diluted in lithium dodecyl sulfate (LDS) sample buffer (Thermo Fisher Scientific) and separated by SDS-PAGE (NuPAGE Novex 10% Bis-Tris gel ...
-
bioRxiv - Neuroscience 2022Quote: ... and reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). Real-time polymerase chain reaction (PCR ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and the precipitated virus pellet was resuspended with 1.1 mL of Opti-MEM (Gibco #31985062) by pipetting and vortexing until fully dissolved ...
-
bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed with TaqMan Fast Virus 1-Step Master Mix (Invitrogen, cat. 4444434) in QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher) ...
-
bioRxiv - Immunology 2023Quote: ... vRNA was reverse transcribed using the TaqMan Fast Virus 1-Step qRT-PCR kit (Invitrogen) and quantified on a LightCycler 480 instrument (Roche ...
-
bioRxiv - Microbiology 2023Quote: ... Virus samples at variable dilutions (1:10 to 1:40,000) were mixed with FluoSphere (Invitrogen) beads (170nm ...
-
bioRxiv - Molecular Biology 2023Quote: ... Virus pellet and cell RNA was isolated using TRIzol according to the manufacturer’s protocol (Ambion) and DNase treated ...
-
bioRxiv - Microbiology 2023Quote: ... Virus-depleted and concentrated cell SN (in PBS) was added to anti-PA (Thermo Fisher) coated beads and incubated overnight ...
-
bioRxiv - Biochemistry 2024Quote: ... Virus generation was performed using the Bac-to-Bac™ expression system (Thermo Fisher Scientific) in Sf9 insect cells ...
-
bioRxiv - Microbiology 2024Quote: ... The virus genome was further amplified by PCR (Phusion High-Fidelity DNA Polymerase, ThermoFisher Scientific) with 10 μL cDNA in each 50 μL reaction and MBTuni-12/13 (5′-ACGCGTGATCAGTAGAAACAAGG ...
-
bioRxiv - Biochemistry 2021Quote: ... Lysates were mixed with NuPAGE LDS Sample Buffer (Invitrogen) plus dithiothreitol (DTT ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysates were diluted in 4X LDS sample buffer (Invitrogen), boiled at 95°C for 5 min ...
-
bioRxiv - Cell Biology 2020Quote: ... crude lysates were reverse transcribed (Thermo Fisher, cat #4368814) before performing qPCR using the GoTAQ qPCR Mastermix (Promega ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell lysates were sonicated (550 Sonic Dismembrator, Fisher Scientific) for 2 min (with one 10 sec pulse at amplitude 5 per 15 sec ...
-
bioRxiv - Molecular Biology 2021Quote: ... Lysates were treated with 2μL of RNAse 1 (Ambion) and incubated at 37°C for 45 minutes ...
-
bioRxiv - Microbiology 2019Quote: ... Lysates were run on Novex Tris-Glycine gels (ThermoFisher), transferred to 0.2 μm PVDF (BioRad ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein lysates were harvested using a magnetic separator (Invitrogen) and were incubated overnight at 4°C under gentle rotation with 10ug of Unc5B antibody (R&D ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein lysates were prepared in 1x LDS (ThermoFisher, NP0007) buffer with 100 mM DTT and heating at 70°C for 10 min ...
-
bioRxiv - Cancer Biology 2020Quote: Lysates were quantified by BCA assay (Thermo Fisher Scientific) and equal protein amounts were run onto SDS-PAGE gels ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein lysates were quantified by BCA kit (Thermo Fisher) and 20-30μg of protein were loaded per lane ...
-
bioRxiv - Cell Biology 2021Quote: RNA from cell lysates was extracted using Trizol (Invitrogen, USA ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysate was precleared with Pierce ProteinG Agarose (Thermo Fisher), and immunoprecipitated with Roche anti-GFP Mouse antibody bound on Pierce protein G agarose ...
-
bioRxiv - Cell Biology 2020Quote: ... cell lysates were obtained in RIPA buffer (ThermoFisher Scientific) supplemented with Complete protease inhibitor cocktail (Roche ...
-
bioRxiv - Genomics 2022Quote: ... and lysates incubated with either glutathione agarose (Thermofisher Scientific) or Ni-NTA resin (Thermofisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... lysates were treated with RNase I (Thermo-Fisher Scientific) at final concentration of 0.25U/μL for 10 min at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lysates were loaded onto 10% Tris-Glycin (Thermo Fisher) for gel electrophoresis ...
-
bioRxiv - Cell Biology 2022Quote: ... The lysate was mixed with 4x loading buffer (Invitrogen) and BME to 10% ...
-
bioRxiv - Biochemistry 2019Quote: ... Lysate was added to 4x native sample buffer (Invitrogen), run on a NativePAGE gel (Invitrogen) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein lysates were denatured in LDS sample buffer (Invitrogen) and sample reducing agent containing DTT (Invitrogen ...
-
bioRxiv - Neuroscience 2019Quote: Lysates were quantified (BCA Protein kit #23225 Thermo Scientific), following supplier protocol.
-
bioRxiv - Cancer Biology 2021Quote: ... lysates were incubated with Co-IP Dynabeads(tm) (Invitrogen) conjugated to anti-pERK5 antibody (Cell Signaling ...