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Citations for Thermo Fisher :
351 - 400 of 3412 citations for Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... cells were washed 3 times and supplemented with virus production serum-free media (VP-SFM, Gibco) containing 0.6 µg/ml trypsin ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... DEPC treated water and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Applied Biosystems, 850 Lincoln Centre Drive ...
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bioRxiv - Immunology 2021Quote: Freshly isolated thymocytes or virus-transduced thymocytes were incubated with 4 μM Indo-1-AM (Invitrogen) in TCM at 37°C for 40 min ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Clarified virus working stock supernatant was overlaid onto a 25% sucrose-NTE (100nM NaCl (ThermoFisher Scientific), 10mM Tris (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Extracted RNA was amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturers’ protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Reprogramming to pluripotency was carried out using the CytoTune 2.0 sendai virus kit (Thermo-Fisher Scientific) to overexpress four transcription factors (OCT4 ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated virus stocks in reduced-serum DMEM (2% FBS) supplemented with 50mM HEPES buffer (Gibco #15630080) were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... unbound virus was removed by washing three times with PBS (Life Technologies, Carlsbad, California, United States). Next ...
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bioRxiv - Microbiology 2020Quote: ... The virus in the growth medium was recovered by Polyethylene Glycol 8000 (PEG-8000, Fisher Scientific) precipitation ...
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bioRxiv - Biophysics 2020Quote: Virus was attached to a gold-coated probe using dithiobis succinimidyl propionate (DSP) linkers (Thermo Scientific). Dithiobis succinimidyl propionate (DSP ...
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bioRxiv - Cell Biology 2020Quote: ... and sendai-virus iPSCs were cultured on matrigel-coated plates (Geltrex™ LDEV-Free, ThermoFisher Scientific) in Essential 8™ medium (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen) with oligo(dT ...
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bioRxiv - Microbiology 2020Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was reverse transcribed using Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific), in the presence of oligo (dT)15 primers ...
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bioRxiv - Genomics 2021Quote: ... we reprogrammed fibroblast samples from 139 iPSCORE individuals using non-integrative Cytotune Sendai virus (Life Technologies) and the 149 iPSCs (7 subjects had 2 or more clones each ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using Taqman Fast Virus 1-step Master Mix (Thermo Fisher Scientific, 4444434) in StepOne Plus real-time PCR thermocycler (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from 100 µl of virus stock using a KingFisher Flex (Thermo Fisher Scientific) in combination with the MagMax Viral Pathogen kit II (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... vRNA was then reverse transcribed using the TaqMan Fast Virus 1-Step qRT-PCR kit (Invitrogen) and quantified on a LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from 100 μl of virus stock using a KingFisher Flex (Thermo Fisher Scientific) in combination with the MagMax Viral Pathogen kit II (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Vero cells were infected with passage 9 virus and overlaid with 1% low melting agarose (Invitrogen) in MEM ...
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bioRxiv - Immunology 2023Quote: ... Media containing virus particles was first concentrated to 20x using 100K MWCO protein concentrator columns (Thermofisher). Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887 ...
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bioRxiv - Cell Biology 2023Quote: High titer virus expressing Flag-WRN was used to infect Hi5 insect cells (Thermo Fisher Scientific) at an MOI of approximately 10 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transduced with Sendai virus (CytoTune – iPS 2.0 Sendai Reprogramming Kit, A16517, Thermo Fisher Scientific) using KOS multiplicity of infection (MOI) ...
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bioRxiv - Microbiology 2023Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Fibroblasts were reprogrammed using the Sendai virus reprogramming method as recommended by the manufacturer (Life Technologies). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... cDNA of the virus genome was generated by reverse transcription (Verso cDNA Synthesis Kit, ThermoFisher Scientific) using MBTuni-12 (5′-ACGCGTGATCAGCAAAAGCAGG ...
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bioRxiv - Genomics 2023Quote: ... Real-time PCR was conducted using TaqMan Fast Virus 1-Step Master Mix from Applied Biosystems in 384-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were infected with virus particles and selected with hygromycin B (200 µg/ml, Invitrogen). For single clone selection ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were quantified by BCA assay (Cat # PI23224, Thermo Scientific). Samples were prepared with NuPage 10x Reducing Agent (Cat# NP0009 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were quantified with Quant-iT Ribogreen assay (Life Technologies) and 60 ug total RNA per sample was incubated with 3 uL RNase I (Epicentre #N6901K ...
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bioRxiv - Cell Biology 2020Quote: ... Lysate concentration was measured using Qubit Protein Assay Kit (Invitrogen) and Qubit 4 Fluorometer ...
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bioRxiv - Cell Biology 2020Quote: ... proteins in the lysate were digested by Proteinase K (Ambion), RNA was cleaned up using SPRI beads (GE ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein lysates were loaded with LDS Sample Buffer (Invitrogen, NP0007) and Reducing Agent (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates were then precleared with Protein G Dynabeads (ThermoFisher; 10004D) at 4°C for an hour ...
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bioRxiv - Molecular Biology 2021Quote: Lysates were precleared on Protein G-magnetic Beads (Thermo Fisher) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were precleared with Dynabeads Protein-G (Novex, Life Technologies) for 1hr ...
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bioRxiv - Cell Biology 2022Quote: Lysates were run on 4-12% polyacrylamide gels (NuPage, Invitrogen) for 50 minutes at 180V and transferred to polyvinylidene difluoride (Immobilon-FL ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were pre-cleared using protein-A Dynabeads (Invitrogen, 10002D), 1 hour at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were pre-cleared using protein A beads (Invitrogen). Pre-cleared lysates were immunoprecipitated using anti-lamin B1 antibody and these immunoprecipitated samples were incubated with protein A-sepharose beads (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... the lysate was incubated with 250ul streptavidin resin (Thermo Fisher) for 1.5hr ...
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bioRxiv - Immunology 2021Quote: ... lysates were incubated with streptavidin magnetic beads (Pierce Thermo Fisher) for 1h at room temperature on a rotator to bind biotinylated proteins ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells lysates were reverse transcribed using Superscript™ III (Invitrogen) and pre-amplified for 20 rounds using a custom-designed set of target-specific primers ...
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bioRxiv - Microbiology 2020Quote: ... RNA from cell lysates were collected using Trizol reagent (Invitrogen). RNA was then extracted from Triazol using the Direct-zol RNA Miniprep Plus kit (Zymo Research #R2072 ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were analyzed with SDS – polyacrylamide gel electrophoresis gels (Invitrogen). Proteins were transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... Whole cell lysates were added to His beads (Thermo Scientific HisPur Ni-NTA Magnetic Beads ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were isolated using RIPA buffer (ThermoFisher Scientific) in the presence of a protease inhibitor mixture (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were treated with RNase I (1:25 dilution; Ambion) for 3 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... and the cleared lysate bound to Ni-NTA resin (Invitrogen) overnight at 4 °C ...