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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Ltbr-Biotin (ThermoFisher) as well as CD31- BUV737 ...
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bioRxiv - Cell Biology 2023Quote: ... Biotin (31822; Invitrogen). The VECTASTAIN Elite ABC system (Vector Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... Knockdown of the protein was monitored by western blotting against the V5×3 epitope using α-V5 mouse primary antibody (Invitrogen / Thermo Fisher Scientific – 1:1000). Oligonucleotides used for PCR amplification are given in Table S4.
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bioRxiv - Cancer Biology 2022Quote: ... 1 mg protein for each condition was incubated with antibody-coated Dynabeads Protein G (Invitrogen, 1003D). Antibody dilutions are provided in table S3 ...
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bioRxiv - Cell Biology 2020Quote: ... 130 ng of total RNA was labeled with biotin using the FlashTag Biotin HSR RNA Labeling Kit (Applied Biosystems) according to manufacturer’s procedure ...
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bioRxiv - Neuroscience 2022Quote: ... interference from endogenous biotin and/or streptavidin-binding activity was moreover reduced by Avidin/Biotin Blocking kit (Thermo Fisher). Tissue sections were incubated overnight in a humidified chamber with following primary antibodies ...
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bioRxiv - Physiology 2019Quote: ... 7 mg of protein were incubated with 343 μM Maleimide-PEG2-biotin (Thermo Fisher Scientific, #21901BID) at room temperature for 30 minutes with agitation by precipitating the alkylated protein with 1 mL of 100% cold acetone by incubating at −20°C for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were dialyzed into bicarbonate buffer and biotinylated using EZ-LinkTM Sulfo-NHS-LC-Biotin (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... Antibody-bound chromatin was incubated with Protein G Dynabeads (Invitrogen, 10004D) for 4 hours at 4’C and eluted in Tris buffer (10mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... FLAG (M2) antibody was coupled to protein G dynabeads (Thermo Scientific). The RNA-protein complexes were immunoprecipitated over night at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... antibody serum was pre-incubated with Protein G coupled dynabeads (Invitrogen) in 1:1 ratio for 2h at 4°C on a rotating wheel ...
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bioRxiv - Neuroscience 2019Quote: The anti-HRI antibody was conjugated with Dynabeads (protein G, Invitrogen) o.n ...
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bioRxiv - Plant Biology 2022Quote: ... The chromatin-antibody complex was recovered with protein A Dynabeads (Invitrogen) and washed ...
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bioRxiv - Genetics 2020Quote: ... 10 µg of antibody was bound to Dynabeads Protein A (Invitrogen) following manufacturer guidelines at 4 °C for 4 h ...
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bioRxiv - Immunology 2023Quote: ... Anti-flag antibody was pre-incubated with protein G Dynabeads (Invitrogen), and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... antibody was conjugated to Dynabeads Protein G (Thermo Fisher Scientific 10003D) at room temperature for 2 hr ...
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bioRxiv - Molecular Biology 2021Quote: ... alkyn-biotin conjugate (PEG4 carboxamide-Propargyl Biotin, Invitrogen, Cat n° B10185), 14 μl of 5 mM stock solution DMSO ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
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bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Immunology 2022Quote: Following fluorescent dye- or biotin-conjugated antibodies (BioLegend, San Diego, CA, USA; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... biotin-labeled anti-rabbit secondary antibody (1:200, Invitrogen, REF 65-6140, LOT WA314056), and an avidin-linked peroxidase complex (AB-HRP kit #PK-4000 ...
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bioRxiv - Biophysics 2022Quote: ... Protein was liquated to 3 replicate wells of a 384-well–plate (Thermo Scientific Nunc), and plates were incubated at 37 °C for 60 h with shaking ...
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bioRxiv - Biophysics 2022Quote: ... Protein was liquated to 3 replicate wells of a 384-well–plate (Thermo Scientific Nunc), and plates were incubated at 37 °C for 60 h with shaking ...
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bioRxiv - Bioengineering 2019Quote: ... Protein separation was done through electrophoresis using a NuPAGE 3-8% tris-acetate gel (ThermoFisher) with wells loaded with 10 µg of protein ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 μg of protein was separated on NativePAGE 3-12% Bis/Tris gel (ThermoFisher, BN1003BOX) and semi-dry blotted to a nitrocellulose membrane and detected as described above.
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bioRxiv - Cell Biology 2023Quote: ... and 3 mg of protein lysate was incubated with Dynabeads MyOne Streptavidin C1 (ThermoFisher Scientific) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the purified RNAs were labeled with biotin using a Pierce RNA 3’ End Biotinylation kit (20160, Thermo Scientific) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified PCR products were 3’-end-labelled with biotin following the manufacturer’s instruction (Thermo Fisher Scientific, USA). The DNA-protein binding reactions were performed according to the manufacturer’s instructions (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 3 µg of proteins measured with the Micro BCA Protein Assay Kit (Thermo Scientific) following the instructions of the supplier ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were resolved in 3-12% and NativePAGETM (Invitrogen) gels ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed for 3-4 days and DiD (Thermo Fisher Scientific-Molecular Probes L7781 ...
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bioRxiv - Molecular Biology 2024Quote: ... Knockdown of the protein was monitored by western blotting against the V5×3 epitope using α-V5 mouse primary antibody (Invitrogen / Thermo Fisher Scientific – 1:1000). Oligonucleotides used for PCR amplification are given in Table S4.
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bioRxiv - Cancer Biology 2021Quote: ... Biotin labelling of the nucleosome assembly was conducted using the EZ-Link™ Psoralen-PEG3-Biotin kit (Thermo Scientific™) as described in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotin was attached to cross-linked RNA duplexes by incubating with 150 µM Click-IT Biotin sDIBO Alkyne (Life technologies) under constant agitation at 37 °C for 1.5 hours ...
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bioRxiv - Immunology 2022Quote: ... Purified DEL was conjugated to biotin at a 1:2 molar ratio using Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) according to the manufacturer’s directions and incubated for 3 hours on ice ...
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bioRxiv - Genomics 2020Quote: ... Chromatin-antibody complexes were captured for at least 3 hr using a mix of Protein A and G Dynabeads (Invitrogen). The captured immunoprecipitated complex were washed 10 min each with sonication buffer (50 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... The same recombinant Cdk5/p25 protein used for the MST assays was labeled with biotin using EZ-LinkNHS-PEG4-Biotinylation Kit (Thermo Scientific, Waltham, MA) and excess reagent was removed with a spin desalting column as per the manufacturer’s instruction ...
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bioRxiv - Biophysics 2021Quote: ... was biotinylated using biotin-NHS (Thermo Scientific, catalog number: 21343). Flow chambers for microscopy were assembled by sandwiching a piece of double-sided tape between a slide and a plasma cleaned coverslip (Fisherbrand ...
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bioRxiv - Biophysics 2022Quote: ... Biotinylation level was quantified using a biotin quantification kit (Thermofisher). The oligo-DNA labeling density was estimated by measuring the band intensities from SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... EZ-Link™ SulfoNHS-SS-biotin was from Thermo Fisher Scientific (D21331) ...
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bioRxiv - Cancer Biology 2020Quote: ... and free biotin was removed by ZebaSpin Desalting Columns (ThermoFisher) following manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... triethylammonium salt (biotin-x-DHPE) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... was biotinylated with Sulfo-NHS-LC-LC Biotin (ThermoFisher, A35358). Excess biotin was removed from each with 3-kDa-cutoff centrifugal filter units (Amicon) ...
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bioRxiv - Bioengineering 2023Quote: Uricase was biotinylated using EZ-link NHS-biotin (Thermo Scientific). Unreacted NHS-biotin was removed using Zeba spin desalting columns ...
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bioRxiv - Bioengineering 2024Quote: Rasburicase was biotinylated using EZ-link NHS-biotin (Thermo Scientific). Unreacted NHS-biotin was removed using Zeba spin desalting columns ...
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bioRxiv - Bioengineering 2020Quote: ... After incubation with excess streptavidin and biotin to block endogenous biotin (Endogenous Biotin Blocking Kit; Thermo Fisher Scientific, Waltham, MA), 5 µM of biotinylated HCDR3-construct was incubated on tissue overnight at 4°C ...
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bioRxiv - Genomics 2023Quote: ... These cells were cultured for 24 hours with either anti-CD3 biotin (Miltenyi)/anti-CD28 biotin (Miltenyi) or anti-CD3 biotin (Miltenyi)/aCD6 biotin (LSBio) on streptavidin coated 96 well plates (ThermoFisher) to yield 50% CD69 expression (antibody amount required determined by prior experimentation) ...
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bioRxiv - Genomics 2023Quote: ... PBMCs were cultured for 24 hours with either anti-CD3 biotin (Miltenyi)/anti-CD28 biotin (Miltenyi) or anti-CD3 biotin (Miltenyi)/aCD6 biotin (LSBio) on streptavidin coated 96 well plates (ThermoFisher) to yield 50% CD69 expression on CD4+ T cells (antibody amount required determined by prior experimentation) ...