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Citations for Thermo Fisher :
351 - 400 of 8899 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Restriction digest products were subjected to gel electrophoresis in 1% agarose gel + 1X SYBR Safe DNA Gel Stain (Invitrogen). DNA bands of digested DNA were excised and purified using NucleoSpin Gel and PCR Clean-Up kit (Machery-Nagel ...
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bioRxiv - Plant Biology 2019Quote: The GAL4 DNA-binding domain vector pGBDKT7-GWRFC was constructed by insertion of the Gateway reading frame cassette RfcC (Invitrogen) into the Klenow-Fragment-treated NdeI-SalI sites of pGBKT7 (pGBDKT7 ...
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bioRxiv - Biophysics 2021Quote: ... The resulting LNPs were dialyzed overnight against sterile PBS pH 7.4 using 10 kDa cut-off Slide-A-Lyzer G2 dialysis cassettes (Thermo Scientific). For NMR ...
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bioRxiv - Cancer Biology 2021Quote: ... The purified proteins were dialyzed against PBS using 3.5 kDa cut-off Slide-A-Lyzer Dialysis Cassettes (Thermo Scientific #66330). The protein concentration was measured by bicinchoninic acid (BCA ...
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bioRxiv - Biochemistry 2020Quote: Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The resultant gRNA cassettes were introduced into the pMpGE010 vector using Gateway LR Clonase II Enzyme Mix (Thermo Fisher Scientific). To express Citrine under regulation of the MpBLD10 promoter ...
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bioRxiv - Molecular Biology 2019Quote: ... To construct the terminator-test plasmid a p.syn expression cassette (Anice Sabag-Daigle and Charles J. Daniels, in preparation) synthesised by lifetechnologiesTM (Thermofisher Scientific) was introduced via NotI/EcoRI into pTA231 (43) ...
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bioRxiv - Developmental Biology 2022Quote: ... exons 2-4 and all of exon 5 except for the 3’ 83 bps with a neomycin phosphotransferase cassette in a 9433 bp genomic subclone obtained from a C57BL/6J BAC (RP23-128H6; Invitrogen). The vector was introduced into Bruce4 (mixed C57Bl/6N:C57BL/6J ...
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bioRxiv - Molecular Biology 2021Quote: ... Smt3 protein was eluted with 250 mM imidazole before being dialyzed using a G2 Slide-a-Lyzer cassette (Thermo Fisher) with a 10 kDa cut-off ...
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bioRxiv - Microbiology 2019Quote: ... peak fractions from the second sucrose gradient were combined and dialysed using a 10,000 molecular weight cut-off Slide-A-Lyzer dialysis cassette (Thermo Fisher) in 1 litre EM buffer (10 mM HEPES [pH 7.6] ...
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bioRxiv - Cell Biology 2019Quote: ... heh1(735-834) was incubated with GST and GST-Chm7 in a dialysis cassette (3.5K Slide-A-Lyzer, Thermo Scientific) and the binding reaction was dialyzed overnight in a binding buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Bioengineering 2020Quote: ... SH-SWCNTs were dialyzed against water using a Slide-A-Lyzer G2 10 kDa molecular cutoff dialysis cassette (Thermo Scientific) with daily water changes for 1 week ...
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bioRxiv - Cell Biology 2021Quote: Gene deletions were generated by PCR amplification of the locus-specific KanMX cassette from template genomic DNA obtained from the relevant strains of the gene disruption collection (Invitrogen) using primers annealing upstream and downstream of the genomic loci and subsequent transformation into the W303 background ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Fractions containing purified protein were pooled and dialyzed overnight against storage buffer (0.02 M sodium phosphate, pH 7) in a 10 kD cut off dialysis cassette (ThermoFisher, 66380). Purified protein was stored at 4 °C for use within days or at −20 °C for longer-term storage.
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bioRxiv - Plant Biology 2020Quote: ... α-DDM and dialyzed in 25 mM HEPES-NaOH (pH 7.8) using Slide-A-Lyzer 20K MWCO G2 Dialysis Cassettes (Thermo Fisher). Dialysis was performed at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Previously built sgRNA cassettes were then individually cloned into the Sa_CBE plasmid through a LR Gateway reaction (ThermoFisher Scientific, USA). The resulting plasmids were checked by restriction digestion and Sanger sequencing (Fig 3B and S2 Table 1).
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bioRxiv - Immunology 2019Quote: ... expressing shRNA targeting human CACNA1F gene or pGFP-V-RS vector with scrambled shRNA cassette (control) was transfected into Jurkat T cells by Neon electroporation system (Invitrogen) according to manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... DTT concentration in the buffer was reduced by dialysis against TEDG buffer with DTT 0.1 mM at 4 ºC overnight using a 3 KDa pore size dialysis cassette (ThermoFisher Scientific). Finally ...
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bioRxiv - Genomics 2022Quote: ... and dialyzed against 1 L of PB in a 3 ml Slide-A-Lyzer™ Dialysis Cassettes (Thermo Fisher Scientific) at RT for 3 hrs ...
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bioRxiv - Microbiology 2022Quote: ... The eluate fractions for each protein were then combined, added to a Slide-A-Lyzer Dialysis Cassette (Extra Strength, 10kDa MWCO, 0.5-3mL capacity) (Thermo Scientific), and dialyzed in PBS at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Peak fractions were then pooled and dialyzed against PBS in 10,000 molecular weight cutoff (MWCO) dialysis cassettes (Thermo Fisher Scientific). After dialysis ...
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bioRxiv - Microbiology 2022Quote: ... Peak fractions were then pooled and dialyzed against PBS in 10,000 molecular weight cutoff (MWCO) dialysis cassettes (Thermo Fisher Scientific) to remove excess HA peptides ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Evolutionary Biology 2022Quote: ... SpCas9 gRNA expression cassettes were cloned on TOPO vectors using manufacturing recommendations (Zero Blunt® TOPO®, Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: pMT-GW-BioID-HA-puro was generated by digesting pGWvectorBirA(R118G)-HA with StuI and PmeI to liberate the Gateway Cassette (Invitrogen) together with the BioID-HA fragment ...
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bioRxiv - Microbiology 2021Quote: ... and dialyzed against sterile milli-Q H2O (∼300X’s the volume of the sample) using a 3.5K MWCO Slide-A-Lyzer® Dialysis Cassette (Thermo Scientific). Samples were dialyzed for ∼18 h at room temperature with H2O changes at 2 and 4 hours ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were then pooled and dialyzed against PBS in 10,000 molecular weight cutoff (MWCO) dialysis cassettes (Thermo Fisher Scientific) to remove excess HA peptide ...
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bioRxiv - Cancer Biology 2021Quote: ... ORF was then recombined from the entry vector into a lentiviral vector containing a GPS (Global Protein Stability) cassette (gift from S. Elledge) using the GATEWAY cloning system (ThermoFisher), resulting in the vector pHAGE-P CMVt N-RIG2 p53(R273C ...
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bioRxiv - Biophysics 2019Quote: All fractions identified to contain the desired protein were combined and placed in a 30 mL dialysis cassette (Thermo Fisher) with 100 units His tagged TEV protease per 1 mg of MBP-fusion protein ...
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bioRxiv - Systems Biology 2019Quote: ... Broccoli nuclear extract was dialyzed in a Slide-A-Lyzer Cassette G2 with a 10,000 MWCO (Thermo Scientific, CA, USA) against 2 changes ...
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bioRxiv - Neuroscience 2021Quote: ... Mito-Dendra2 was a gift from David Chan.The Mito-Dendra2 sequence was inserted to a PiggyBac backbone construct along with a T2A Neomycin selection cassette using the GeneArt assembly kit (Invitrogen). We also used a pgK-Puro-CMV-GFP and pPb-CAG-RFP-Hygro construct cloned in the lab and a PiggyBac vector containing the transposase.
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bioRxiv - Molecular Biology 2019Quote: U2OS 2–6-3 cells containing 200 copies of a LacO-containing cassette (Janicki et al., 2004) were co-transfected with lipofectamine 2000 (Invitrogen) and plasmid DNA for 6 h at 37 °C in an atmosphere of 5% CO2 ...
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bioRxiv - Genetics 2019Quote: ... and thus had not lost silencing and excised the RFP-hphMX cassette (Figure 1A): YPD containing 200μg/mL G418 (Geneticin; Life Technologies) for strains carrying the kanMX cassette or YPD containing 300μg/mL Hygromycin B (MilliporeSigma ...
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miRNA activity contributes to accurate RNA splicing in C. elegans intestine and body muscle tissues.bioRxiv - Molecular Biology 2019Quote: ... The entire cassette was then PCR amplified with Gateway-compatible primers and cloned into pDONR P221 by Gateway BP Clonase reactions (Invitrogen).
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bioRxiv - Biochemistry 2021Quote: ... Nuclear localised FRET barnase was generated by the addition of a N-terminal SV40 NLS sequence to the original cytosolic barnase using a synthesized gene cassette containing the relevant localization sequences (GeneArt (Thermofisher), Waltham ...
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bioRxiv - Cancer Biology 2021Quote: ... These formed HistoGel cell mixtures in nylon bags were placed into individual tissue processing cassettes (ThermoFisher Scientific Catalog No. 1000957), and then were submerged in a plastic pail filled with 10% NBF to simulate pre-tissue-processing time-in-formalin delay before batch tissue processing steps.
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bioRxiv - Cell Biology 2020Quote: ... Hygromycin B resistance cassette was amplified by PCR (primer sequences available upon request) and inserted into KpnI-digested pLenti6.2/V5-DEST (Invitrogen, V36820), replacing the Blasticidin resistance cassette (pLenti6.2_Hygro/V5-DEST) ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were then pooled and dialyzed against PBS in 10,000 molecular weight cutoff (MWCO) dialysis cassettes (Thermo Fisher Scientific). After dialysis ...
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bioRxiv - Microbiology 2021Quote: ... a spectinomycin resistance cassette flanked by 0.8 kb sequences homologous to the sequences flanking perRA was created by gene synthesis (GeneArt, Life Technologies) and cloned into a kanamycin-resistant Escherichia coli vector unable to replicate in Leptospira ...
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bioRxiv - Developmental Biology 2020Quote: ... A pMA-T vector encoding for a DNA in which the DSG domain of Arpp19 sequence was exchanged by the cassette domain (D2-D1 mutant) was synthesized by Geneart (Thermofisher) and sub-cloned into Pet-15 6his vector.
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bioRxiv - Biophysics 2021Quote: ... all plectasin samples were dialyzed into the desired condition using Slide-A-Lyzer™ 2000 MWCO dialysis cassettes (Thermo Fisher) with a buffer exchange after 2 and 4 hours and then continued overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The gRNA-cRNA cassette was mobilized to the hybrid destination vector pDEST:Cpf1&Cas9 using Gateway LR Clonase (Thermo Fisher Scientific) to produce a pCam1300-CRISPR plasmid named pCpf1&Cas9:gRNAs-cRNAs (pMUGW5) ...
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bioRxiv - Plant Biology 2022Quote: ... followed by an LR reaction using a modified pGREEN binary vector with a Gateway cassette upstream of 3xNLS-RFP (Invitrogen). The HD-ZIPI BS:3xNLS-RFP reporter was created by first synthesizing a construct comprised of six copies of the HD-ZIPI binding site (CAATTATTG ...
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bioRxiv - Plant Biology 2022Quote: ... Expression cassettes were subcloned into the pDONR207-NeoR vector(Guyon-Debast et al., 2021) by Gateway™ BP reaction (Invitrogen) as previously described to yield psgRNA#5 ...
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bioRxiv - Immunology 2022Quote: ... was dialyzed for 48 h at 4°C into PBS using 10 kDa MWCO Slide-a-lyzer cassettes (Thermo Fisher) before i.n ...
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bioRxiv - Plant Biology 2022Quote: ... The guide RNA and scaffold-infused fragments (sgRNA-scaffold) were amplified from a custom synthesized cassette (Thermo Fisher Scientific, Inc.). Four sets of primers were used to synthesize a double-stranded sgRNA expression cassette with an overlapping strategy for different targets (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... The upper 90% of the supernatant was extracted and dialysed using Slide-A-Lyzer™ cassettes (Thermo Scientific, Cat. 66330) with a 2 kDa molecular weight cut off ...
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bioRxiv - Biochemistry 2023Quote: ... Conjugates were purified from excess of free antigenic peptides by several dialysis steps at 4 °C against PBS using Slide-A-Lyzer 20,000 MWCO dialysis cassettes (Thermo Scientific). STxB(70C)-SL8 and STxB(70C)-G15F conjugate formation and the absence of remaining free SL8 or G15F after purification were validated by UPLC-MS.
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bioRxiv - Microbiology 2023Quote: ... The kanamycin resistance cassette flanked with 0.8 kb sequences homologous to the adjacent perRA sequences was obtained by gene synthesis (GeneArt, Life Technologies), and subsequently cloned into a kanamycin-resistant E ...
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bioRxiv - Molecular Biology 2023Quote: ... It was then centrifuged at 13,300 rpm and 4 °C for 15 min and the supernatant was transferred to a “Slide-A-Lyzer” G2 Dialysis Cassette’ (3.5K) (Thermo Scientific) and dialyzed against 3 L of dialysis buffer (20 mM Tris-Cl pH 7.3 ...