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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Vacuolar Protein Sorting Associated Protein 26B VPS26B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... At least 400 μg of total protein were incubated overnight with the designated antibody and then with protein G-coupled magnetic beads (Life Technologies) for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... The volume corresponding to 200–300 μg of protein was incubated with the corresponding antibody and Dynabeads Protein G (Life Technologies) overnight at 4°C to form immunocomplexes ...
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bioRxiv - Cell Biology 2021Quote: ... The volume corresponding to 60–100 μg of protein was incubated with the corresponding antibody and Dynabeads Protein G (Life Technologies) overnight at 4°C to form immunocomplexes ...
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bioRxiv - Molecular Biology 2019Quote: ... bakeri worm lysates or EVs were carried out with rat polyclonal anti-exWAGO antibody (raised against full length protein) or rat IgG (control) using protein L magnetic beads (Fisher Scientific). Equivalent amounts of the input ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant (2 mg of proteins) was incubated with 2 μg of target antibodies and 20 μl of Protein A/G Plus Ultralink Resin (Thermo Scientific) for 4 hours at +4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mg of total protein was incubated with the DNA-PKcs monoclonal antibody (25–4) and Protein A/G beads (Thermo Fisher) overnight at 4° C with mixing ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies targeting proteins of interest or IgG isotype control (5ug) were incubated with 20ul of Dynabeads Protein G (Thermo Fisher Scientific) in buffer WB150 (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of antibodies against Piwi and Aubergine were each coupled to 50:50 of protein-A and protein-G Dynabeads (Life Technologies). Lysates were incubated with bead-coupled antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies were captured by 20 µ l of a 50:50 mix of Protein-A and Protein-G Dynabeads (Life Technologies). The beads were washed several times sequentially in the buffers containing low salt (150 mM NaCl) ...
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bioRxiv - Biophysics 2023Quote: ... To immunodeplete Npm2 from Xenopus egg extract purified polyclonal Npm2 antibody (rabbit a-Npm2 IgG, 39) were first cross linked to Protein A beads (Dynabeads™ Protein A, 10001D, ThermoFisher) using Dimethyl pimelidate dihydrochloride (D8388 ...
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bioRxiv - Molecular Biology 2024Quote: Antibody-bound complexes were then captured by incubation with Protein A sepharose CL-4B beads (Amersham, 17-0963-03) for rabbit antibodies or Protein G sepharose 4B beads (Invitrogen, 101241) for mouse antibodies (both 20 μL per 1 mL lysate ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-tagged proteins were detected with HRP-conjugated polyclonal anti-Protein A (Invitrogen, PA1-26853) at 1:5000.
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were resolved alongside a protein ladder (PageRuler Plus Pre-stained Protein Ladder, Thermo Fisher Scientific) on NuPAGE 4-12% BisTris gradient gels using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was quantified using Qubit™ Protein and Protein Broad Range (BR) Assay Kits (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and protein concentration was determined by protein assay (EZQ Protein Quantification Kit; Life Technologies, Carlsbad, CA). Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Vacuolar pH alterations were detected using the 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA, Invitrogen) probe ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations of the subcellular protein fraction lysates were measured using Pierce BCA Protein Assay Kit (ThermoFisher). Samples were boiled for 10 minutes and the extracts were separated by SDS-polyacrylamide gel electrophoresis using precast 4–20% Precise protein gels (ThermoScientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Rab27b protein levels were normalized to total protein levels determined by SYPRO Ruby protein gel stain (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: The protein–protein interaction for endogenous protein was studied by using the Co-Immunoprecipitation Kit (Thermo Scientific). For overexpressed HA-tag proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Synaptosomal protein expression levels were normalized to total protein using No-Stain Protein Labeling Reagent (Invitrogen, A44449). Primary antibodies (Anti-Shank3 (N367/62) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were incubated with Protein-A beads (Thermo Fisher Scientific) conjugated with anti-p53 antibody or Mouse IgG at 4 °C overnight with gentle shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... protein was quantified using the Qubit Protein Assay Kit (Invitrogen). Antibodies used were ...
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bioRxiv - Immunology 2021Quote: ... Total protein was measured by Pierce™ Protein Assay (ThermoFisher). Cytokine levels were standardized to total protein content.
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bioRxiv - Microbiology 2019Quote: ... Protein concentration was determined using BCA protein Assay (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Protein concentration was determined using a BCA protein assay (ThermoFisher). Samples were then reduced in Laemmli buffer and incubated for 5 min at 95°C ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein quantified using BCA Protein Assay Kit (Thermo Fisher) with bovine serum albumin as a protein standard ...
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bioRxiv - Immunology 2022Quote: ... Protein concentration was determined using EZQ protein quantification kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentrations were determined by BCA protein assay (Thermo Fisher) using BSA as a standard ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein concentration was measured via Pierce BCA protein assay (ThermoFisher). Approximately 15 μg supernatant protein was diluted 4:1 in reducing 4X Laemmeli buffer for 1 hour at room temperature and separated at 100V for 1 hour on 10% SDS-PAGE gels ...
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bioRxiv - Neuroscience 2020Quote: ... Protein sample concentrations were determined using Bradford protein assay (ThermoFisher). Protein samples were prepared with 4x LDS (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was assessed by BCA protein assay (Thermo Scientific). To ensure that equal amount of protein undergoes RBD pulldown ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was assessed by BCA protein assay (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Proteins were coimmunoprecipitated using Protein A Dynabeads (Thermo Fisher Scientific) and Western blots were performed as described above (1:1000 used for both anti-Ryk and anti-Pcdh19 primary antibodies for Western blots).
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bioRxiv - Genetics 2021Quote: ... Protein concentration was measured using BCA Protein Assays (Thermo Fisher). Samples were then digested overnight with rLys-C (Promega) ...
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bioRxiv - Physiology 2021Quote: After determining myocardial protein concentrations (Pierce BCA Protein Assay; ThermoFisher) protein aggregate accrual in the heart was measured using a commercially available kit (Proteostat ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was quantified with Qubit Protein assay (Life Technologies) and Pierce BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were estimated using the BCA protein estimation kit (ThermoFisher) and subsequently separated by 10% SDS-PAGE and transferred onto nitrocellulose membrane (Pall Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were measured by BCA protein assay kit (ThermoFisher), equal amounts of proteins were analyzed for western blotting ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined by BCA protein assay (ThermoFisher Scientific) and 20 µg total protein was loaded ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was quantitated with BCA Protein Assay (Thermofisher Scientific) and stored in aliquots at −80 °C until used.
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bioRxiv - Cancer Biology 2020Quote: ... coupled with protein A and protein G beads (Life Technologies) at 4 degrees Celsius overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Equivalent protein concentrations were immunoprecipitated with Protein G DynabeadsTM (Invitrogen) coupled to a mouse anti-biotin primary antibody ...
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bioRxiv - Genetics 2019Quote: ... Protein concentrations were determined by BCA protein assay (Thermo Scientific). Fifty μg of protein was taken from each sample for digestion ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were determined by BCA protein assay (Life Technologies). Equal protein amounts were denatured in SDS buffer (1% SDS ...
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bioRxiv - Microbiology 2020Quote: ... Protein content was measured using Qubit protein assay (Invitrogen Q33212) and lysates were normalized by protein content ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The proteins were immunoprecipitated overnight with Dynabeads Protein G (Invitrogen) interacting anti-AGIA antibody at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Protein concentration was measured using a Qubit protein assay (Invitrogen).
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bioRxiv - Immunology 2020Quote: ... Total proteins were quantified by BCA protein assay (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentrations were determined by BCA protein assay (Thermo Scientific). For in-gel fluorescence detection of nitro-fatty acid protein targets ...
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bioRxiv - Physiology 2021Quote: ... Protein concentration was determined by BCA Protein Assay (Thermo Fisher). Cell lysate ...