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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Tick Borne Encephalitis Virus Envelope Protein Mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Immunology 2024Quote: ... Infection was initiated by adding 100 µl of virus inoculum containing 100.000 PFU (MOI ∼0.5-1) in virus absorption medium (Gibco OptiPro Serum free medium + 1:100 Glutamax + 10 mM HEPES ...
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bioRxiv - Immunology 2020Quote: IL-2/Fc fusion proteins were expressed using the Expi293 expression system according to manufacturer instructions (Thermo Scientific). Proteins were as human IgG1 Fc fused at the N-or C-terminus to human IL-2 through a (G4S)4 linker ...
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bioRxiv - Bioengineering 2021Quote: The purified Fc fusion baits were captured on 96-well protein-G coated plates (Thermo Fisher Scientific 15131). The biotinylated baits were immobilized on 96-well streptavidin-coated plates (Thermo Fisher Scientific 15500) ...
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bioRxiv - Immunology 2023Quote: ... The idiotype antibodies and Fc- fusion protein were conjugated in house with Dylight650 antibody labelling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
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bioRxiv - Biochemistry 2022Quote: Bait and tag proteins (10-15 μg in 100 μL PBS) were immobilised in 96-well MaxiSorb plate (NUNC) o.n ...
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bioRxiv - Immunology 2022Quote: ... Protein expression was confirmed by western blot using a 6x His-Tag HRP conjugated Monoclonal Antibody (Thermo Fisher Scientific). Once verified ...
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bioRxiv - Cell Biology 2020Quote: ... nucleotide fragments encoding the fusion Flag tag protein and full-length of Myocardin and encoding the fusion Myc tag protein and full-length of STAT3 were cloned to mammalian expression vector pCDNA3.1 (Invitrogen) (Flag-pCDNA3.1-Myocardin ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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bioRxiv - Plant Biology 2022Quote: DNA templates of twin-strep-tag (TST) modified D1 and uS2c proteins were obtained from Invitrogen (GeneArt Gene Synthesis). Therefore ...
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bioRxiv - Immunology 2023Quote: ... Proteins containing a His-tag were purified by affinity chromatography using HisPur Ni-NTA resin (Thermo scientific, Cat#88222). Proteins were then eluted with 250 mM imidazole in 50 mM Tris-HCl and 300 mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... All supernatants were harvested via centrifugation and the proteins purified using CaptureSelect C-tag affinity matrix (Thermo Fisher Scientific) on an ÄKTA Pure FPLC system (Cytiva) ...
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bioRxiv - Cancer Biology 2021Quote: ... envelope plasmid pIP/VSV-G (5μg) (ViraPower™ Lentiviral Packaging Mix, Thermo Fisher Scientific) and 5μg lentiviral expression constructs shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5µg psPAX2 packaging plasmid and 1µg pMD2.G envelope plasmid using Lipofectamine 2000 (Invitrogen). Lentiviral supernatant was collected ...
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bioRxiv - Microbiology 2022Quote: ... Cell envelope samples were vitrified using a Vitrobot plunge-freezing machine (Mark IV, ThermoFisher) at room temperature (blot force 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... the cell envelope was stained with wheat germ agglutinin-AlexaFluor488 (WGA-AF488, Life Technologies), which was added to the loading well to a final concentration of 10 μg/mL prior to loading cells into the imaging chamber ...
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bioRxiv - Molecular Biology 2020Quote: ... pMD2-VSVG (envelope vector) and pCDH plasmids co-transfection using Lipofectamine 2000 (#11668019, Invitrogen). Medium containing virus was harvested 48 h after transfection and filtered by 0.45 μm millipore filter ...
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bioRxiv - Genomics 2022Quote: ... psPAX2 packaging plasmids and pMD2.G envelope plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: CH848 SOSIP gp140 envelope production was performed with Freestyle293 cells (ThermoFisher Cat No. R79007). On the day of transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... envelope plasmid pIP/VSV-G (5 µg) (ViraPowerTM Lentiviral Packaging Mix, Thermo Fisher Scientific), 5 µg of lentiviral expression construct shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Cancer Biology 2023Quote: ... and the envelope vector pVSV-G using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 600 ng VSV-G envelope plasmid and 6 μL of Lipofectamine 2000 (Thermo Scientific). Supernatants were collected at 48 and 72 h post-transfection and clarified using 0.45 μm filters ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Pelleted virus was resolved in PBS (Gibco) and stored at −80°C until further usage ...
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bioRxiv - Immunology 2019Quote: ... murine leukemia virus reverse transcriptase (RT; Invitrogen), and random hexamers (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Fibroblasts were reprogrammed using Sendai virus (Invitrogen) in feeder-free conditions on Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... and EmGFP reporter Sendai Virus (A16519, Invitrogen) were part of the Cytotune -iPS 2.0 Sendai Reprogramming kit and EmGFP Sendai Fluorescence Reporter kit ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080] ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Microbiology 2019Quote: ... The viral N-protein was labeled using a primary anti-Tula virus antibody(12) and a secondary anti-rabbit FITC conjugate (Invitrogen, USA). The viral Gc protein was stained using a mouse monoclonal antibody (#H1808-60B ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Cancer Biology 2020Quote: ... the fusion proteins were diluted in mouse serum (Invitrogen) to 200 nM and incubated at 37°C for up to 48 h ...
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bioRxiv - Neuroscience 2020Quote: ... or control mouse IgG and protein G Dynabeads (Invitrogen). Immunocomplexes were eluted by denaturation in NuPAGE LDS sample buffer (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:50,000 ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody (AlexaFluor488 donkey anti mouse IgM 1/400, Life Technologies in Buffer A, 0.1% Saponin 2% FCS) was performed ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were pre-incubated with mouse-Fc block and viability marker prepared in calcium/magnesium-free PBS (Gibco), before staining with fluorescently-conjugated antibodies prepared in staining buffer (2mM EDTA ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β3 (armenian hamster monoclonal, 2C9.G3, Thermo Fisher Scientific, 13-0611-81; FC 1:200), anti-mouse integrin α5 (rat monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β1 (Armenian hamster monoclonal, Hmb1-1, Thermo Fisher Scientific, 11-0291-82; FC 1:300), anti-mouse integrin β3 (armenian hamster monoclonal ...
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bioRxiv - Bioengineering 2023Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:10,000 ...
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bioRxiv - Immunology 2021Quote: ... Cell pellets were then incubated with a mouse Anti-V5 tag monoclonal antibody (1.2 mg mL−1; Thermofisher Scientific) in PBS 0.1% BSA for 45 min at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Anti-Myc-tag mouse antibody Magnetic beads were added and immunoprecipitated according to the manufacturer’s protocol (MBL and ThermoFisher). Western blotting was performed according to standard techniques.
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bioRxiv - Microbiology 2024Quote: ... Membranes were then incubated for 1 h at room temperature with the following primary antibodies: mouse anti-His-Tag (dilution 1:1,000, clone HIS.H8, Invitrogen MA121315), mouse anti-Flag (dilution 1:1000 ...
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bioRxiv - Microbiology 2022Quote: Total RNA prepared from the homogenates of the ticks and the blood samples collected from hedgehogs’ heart were extracted using TRIzol reagent (Thermo Fisher Scientific, USA) or the RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... 100 µL of L15C300 was added in a microtube for each tick before preparation on Fisherbrand™ Superfrost™ Plus microscope slides (Fisher Scientific) using a Epredia™ Cytospin™ 4 Cytocentrifuge (Thermo Scientific).
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bioRxiv - Immunology 2023Quote: ... Biopsy samples using 3 mm biopsy punches (Miltex, USA) were collected at the tick bite sites (1 site/animal) and stored in RNAlater Stabilization Solution (ThermoFisher, Waltham, MA, USA). During the partial feeding phase of ticks (3 days after infestation of mice) ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were detected by Coomassie Blue staining or Western blotting using anti-His6-tag antibodies (Thermo Fisher Scientific MA1-21315).
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bioRxiv - Immunology 2021Quote: ... The efficiency of the expression and purification of recombinant proteins were evaluated by 12% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:3,000) (Thermo Scientific) and HRP-conjugated goat anti-mouse IgG (1:10,000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein with an N-terminal His10 tag was expressed in Sf9 cells following the Bac-to-Bac baculovirus system (Invitrogen). Cells were collected and solubilized to extract protein in the buffer containing 25 mM MES pH 6.0 ...
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bioRxiv - Microbiology 2019Quote: ... 1 nM of CAT protein (Chloramphenicol acetyltransferase, containing a 6×His tag at C-terminus) expressed in the same baculovirus-expression system (ThermoFisher) was used to coat plate wells ...